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resident and infiltrating immune cells

Báo cáo khoa học: Differential regulation of fatty acid amide hydrolase promoter in human immune cells and neuronal cells by leptin and progesterone pdf

Báo cáo khoa học: Differential regulation of fatty acid amide hydrolase promoter in human immune cells and neuronal cells by leptin and progesterone pdf

Báo cáo khoa học

... hydrolase promoter inhuman immune cells and neuronal cells by leptin and progesteroneMauro Maccarrone1,2, Valeria Gasperi3, Filomena Fezza1, Alessandro Finazzi-Agro`3 and Antonello Rossi31Department ... seems noteworthy that leptin [42,43] and progesterone [44,45] play roles in survival, death and proliferation of immune and neuronal cells; processes inwhich AEA and the endocannabinoid system are ... of AMT, NAT and PLD in thesame cells (Figs 1A,B,D and data not shown). It should berecalled that also CHP100 cells are d evoid o f c annabinoidreceptors [18].Both U937 and CHP100 cells have...
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Báo cáo y học:

Báo cáo y học: " Effect of Weight Reduction on Cardiovascular Risk Factors and CD34-positive Cells in Circulatio"

Y học thưởng thức

... of cells after the diet to the percentage of cells before the diet) and the percentage of body fat lost by each subject during the study. A correlation also exists between CD34+ cells and ... weight adults, and the colony-forming capac-ity of these cells is blunted [17, 18]. Alterations in en-dothelial cells and EPC function associated with obe-sity precede atherosclerosis and thrombosis ... blood–derived cells from which endothelial cells in culture were developed were described as cells expressing CD34 antigen. It has been hypothe-sized that endothelial progenitor cells and hemato-poietic...
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Tài liệu Báo cáo khoa học: Development of a new method for isolation and long-term culture of organ-specific blood vascular and lymphatic endothelial cells of the mouse pdf

Tài liệu Báo cáo khoa học: Development of a new method for isolation and long-term culture of organ-specific blood vascular and lymphatic endothelial cells of the mouse pdf

Báo cáo khoa học

... dishes and cultured at 33 °C (day 0 in Fig. 1).For the initial 2 weeks, primary cells, including bothtsA58T Ag-negative cells (primarily fibroblasts) and tsA58T Ag-positive cells, proliferated, and ... isolation and maintenance of murine endothelial cells from variousdevelopmental stages and locations is important fordissecting molecular and cellular mechanisms of endo-thelial development and ... in these cells. Mes-enteric BECs and LECs (Fig. 5A,B) were also obtainedby the same strategy as illustrated in Figs 1 and 4, and were treated with VEGF-A or VEGF-C (Fig. 5C).MAPK and Akt phosphorylation...
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Tài liệu Báo cáo khoa học: A peptide containing a novel FPGN CD40-binding sequence enhances adenoviral infection of murine and human dendritic cells doc

Tài liệu Báo cáo khoa học: A peptide containing a novel FPGN CD40-binding sequence enhances adenoviral infection of murine and human dendritic cells doc

Báo cáo khoa học

... detectablehomology to CD40 ligand (data not shown) and thatenhances adenoviral infection of CD40-positive cells of bothhuman and murine origin, including DC and B cells resistant to infection ... as human and murine B cells. DC,LCL,L10A,Blasts:theselabelsrefer, respectively, to day 8 human DC, EBV-immortalized human B cells, murine B lymphoma L10A cells and murine LPS blasts. Cells (105) ... thatbinds to CD40 and enhances adenoviral infection of mouse and human DC and B lineage cells. Materials and methodsCell linesEpstein–Barr virus- (EBV-) transformed human B cells wereprovided...
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Tài liệu Báo cáo khoa học: Constitutive oligomerization of human D2 dopamine receptors expressed in Spodoptera frugiperda 9 (Sf9 ) and in HEK293 cells Analysis using co-immunoprecipitation and time-resolved fluorescence resonance energy transfer pdf

Tài liệu Báo cáo khoa học: Constitutive oligomerization of human D2 dopamine receptors expressed in Spodoptera frugiperda 9 (Sf9 ) and in HEK293 cells Analysis using co-immunoprecipitation and time-resolved fluorescence resonance energy transfer pdf

Báo cáo khoa học

... dopamine,R-(–)propylnorapomorphine, and raclopride did not affectoligomerization of D2L and D2Sin Sf9 and HEK293 cells. Human D2dopamine receptors can therefore form consti-tutive oligomers in Sf9 cells and in HEK293 cells ... the dopamineD2L and D2Sreceptors can form constitutive homo- and hetero-oligomers in two expression systems (Sf9 and HEK293 cells) and these are not regulated by receptorligands. Our study ... canalso form hetero-oligomers in Sf9 cells. Time-resolvedFRET with europium and XL665-labelled antibodies wasappliedtowholeSf9 cellsandtomembranesfromSf9 cells expressing epitope-tagged D2receptors....
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Tài liệu Báo cáo khoa học: Infrared spectroscopy as a tool for discrimination between sensitive and multiresistant K562 cells doc

Tài liệu Báo cáo khoa học: Infrared spectroscopy as a tool for discrimination between sensitive and multiresistant K562 cells doc

Báo cáo khoa học

... to the asymmetric stretching of CH3 ,and the band at 2 873 cm)1to its symmetric mode. T he bands at2926 and 2853 cm)1can be a ssigned to an asymmetric and symmetric stretching mode of CH2, ... between 1300 and 900 cm)1arise mainlyfrom phosphate associated with nucleic acids, i.e. DNA and RNA. The a bsorption bands a t 1245 a nd 1087 cm)1are c haracteristic o f asymmetric and symmetric ... plot of PCA-reduced s pectra of K5 62 cells. Resistant K562 cells (39 spectra, black stars), sensitive K562 cells (39 sp ectra, circles) o f the training (full) and test s et (empty). Thepercentage...
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Control of Innate and Adaptive Immune Responses during Infectious Diseases ppt

Control of Innate and Adaptive Immune Responses during Infectious Diseases ppt

Sức khỏe giới tính

... 1626–9.12J. AlibertiNK cells, JG CD3+ T cells, noncytolytic NK cells, lymphoid tissue inducer cells, and skin-homing IL-13+ T cells. The receptor for IL-22 is distinct and consists of IL-22R, ... transmission. On the other hand, hosts need a robust and extended immune response in order to expand memory cells. This critical balance is where the co-evolution between host and pathogens lies. This ... activates NK cells to produce IFN-J and drives the proliferation of type 1 CD4+ and CD8+ T cells, which produce even more IFN-J. IFN-J-producing cells is a central component to induce and maintain...
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Báo cáo Y học: Group IID heparin-binding secretory phospholipase A2 is expressed in human colon carcinoma cells and human mast cells and up-regulated in mouse inflammatory tissues doc

Báo cáo Y học: Group IID heparin-binding secretory phospholipase A2 is expressed in human colon carcinoma cells and human mast cells and up-regulated in mouse inflammatory tissues doc

Báo cáo khoa học

... was detected in KM12,KM20L2 and WiDr cells. COX-1 was highly expressed inHT29 and WiDr cells and weakly expressed in KM20L2 cells. COX-2 was detected only in WiDr cells. The twoterminal PGE2-biosynthetic ... mast cells, human mast cells developed in the presence of SCF and IL-6 from cordblood cells [38] expressed only sPLA2-IID, but not the othersPLA2s including -IB, -IIA, -IIE, -IIF, -V and ... by sPLA2-IIA and -V(net 4–5%) (Fig. 3A).When cells expressing sPLA2-IID were cocultured withthose coexpressing COX-2 and mPGES and then stimulated(transcellular prostaglandin biosynthesis...
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Báo cáo khoa học: Transport of the phosphonodipeptide alafosfalin by the H+/peptide cotransporters PEPT1 and PEPT2 in intestinal and renal epithelial cells doc

Báo cáo khoa học: Transport of the phosphonodipeptide alafosfalin by the H+/peptide cotransporters PEPT1 and PEPT2 in intestinal and renal epithelial cells doc

Báo cáo khoa học

... H+/peptidecotransporters PEPT1 and PEPT2 in intestinal and renal epithelial cells Jana Neumann1, Mandy Bruch1, Sabine Gebauer2 and Matthias Brandsch11Membrane Transport Group, Biozentrum and 2Institute ... inhibition constants and the competitive nature ofinhibition were confirmed in Dixon-type experiments. Caco-2 cellsand SKPT cells were also cultured on permeable filters:apical uptake and transepithelial ... plus tracer binding, of 19.2% and 5.1% weredetermined with an excess amount of unlabeled Gly-Sar(31.6 mMGly-Sar for Caco-2 cells and 20 mMGly-Sar forSKPT cells) and subtracted from total uptake...
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Báo cáo khoa học: Tec family kinases: Itk signaling and the development of NKT ab and cd T cells potx

Báo cáo khoa học: Tec family kinases: Itk signaling and the development of NKT ab and cd T cells potx

Báo cáo khoa học

... conventionalCD4+ and CD8+T cells, and normal or increaseddevelopment of CD4+ and CD8+T cells, which havean activated memory-cell-like phenotype ([8–12],reviewed in [13] and [14]). These cells have ... devel-opment and function of iNKT cells and cd T cells seems to be quite different from conventional abT cells. This minireview focuses on this aspect of Itk,its role in the development and function ... iNKT and NKT-like cd T cells. Itk and i NKT cell developmentNKT cells are a subset of innate T cells characterized bytheir expression of the NK1.1 marker along with the abTCR. Although these cells...
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Báo cáo khoa học: Identification of proNeuropeptide FFA peptides processed in neuronal and non-neuronal cells and in nervous tissue potx

Báo cáo khoa học: Identification of proNeuropeptide FFA peptides processed in neuronal and non-neuronal cells and in nervous tissue potx

Báo cáo khoa học

... cord.NPFF-related peptides from cells and tissues were extracted and purified by RP-HPLC, assessed by radioimmunoassay and identified by using on-line capillary HPLC/nanosprayion trap tandem mass spectrometry ... at94 °Cand30sat68°C. PCR products were cloned inpEGFPn3 (Clontech) and sequenced on both strands.Tissue extractionMouse spinal cord tissue. Animals were handled inaccordance with standard ... should help to understand their rolein neurotransmision. Thus, we investigated the processing ofproNPFFAin SH-SY5Y human neuroblastoma cells and COS-7 (nonneuronal) cells transiently transfected...
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Báo cáo Y học: A new siglec family member, siglec-10, is expressed in cells of the immune system and has signaling properties similar to CD33 docx

Báo cáo Y học: A new siglec family member, siglec-10, is expressed in cells of the immune system and has signaling properties similar to CD33 docx

Báo cáo khoa học

... between 48 and 60 h post-transfection. Blood cells and cell lines were labeled with calcein-AM (5 mL per108 cells) for 30 min at 37 8C. Red blood cells, mixed whiteblood cells, Ramos and Daudi ... other blood cells including CD281 cells and CD31 cells (data not shown).The antibody was also used to probe Western blots ofextracts from several cell lines and purified peripheral blood cells. The ... withadherent transfected COS7 cells, a binding assay withadherent endothelial cells was also performed. HUVEC cells (ATCC) were plated and grown to confluence in96-well plates. COS7 cells were transfected...
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Báo cáo y học:

Báo cáo y học: " Helicobacter pylori induces mitochondrial DNA mutation and reactive oxygen species level in AGS cells"

Y học thưởng thức

... Cytb gene and Hp in-duced mutations in D-Loop region were closely re-lated to the bacterial virulence and the endogenous ROS level. Materials and metho ds Cells and Hp Strains AGS cells were ... DHR-123 and 50 µmo l/ L H2O2 for 24 h. Detection of ROS using Flow cytometry Cells were washed with PBS once. After trypsin digestion, AGS cells were re-suspended in PBS and 1×104 viable cells ... for both the heavy and light strands of the mtDNA, and contains essential transcription and rep -lication elements. The D-loop region is a hot spot for mtDNA alterations and the nucleotide...
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