rab1 40 in cos 7 cells and generation of flag rab panels
Báo cáo khoa học: Curcumin suppresses the dynamic instability of microtubules, activates the mitotic checkpoint and induces apoptosis in MCF-7 cells ppt
... vinblastine in inhibiting MCF -7 cell proliferation Curcumin affected the localization of the kinesin protein Eg5 Because curcumin produced monopolar spindles in MCF -7 cells, we examined the effect of ... with vinblastine for inhibition of MCF -7 cell proliferation Curcumin, paclitaxel and vinblastine inhibited MCF -7 cell proliferation with median inhibitory doses of 15 ± lm, 40 ± nm and 17 ± 10 ... instability of individual microtubules in live MCF -7 cells Life-history traces of individual microtubules in MCF -7 cells in the absence (A) and presence of (B) lM curcumin and (C) 12 lM curcumin, respectively...
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báo cáo hóa học:" Generation in vivo of peptide-specific cytotoxic T cells and presence of regulatory T cells during vaccination with hTERT (class I and II) peptide-pulsed DCs" pot
... of 23 (page number not for citation purposes) Journal of Translational Medicine 2009, 7: 18 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 cells in priming CD8+ T cell responses against ... Enk AH: Depletion of CD4+CD25+ human regulatory T cells in vivo: kinetics of Treg depletion and alterations in immune functions in vivo and in vitro Int J Cancer 20 07, 120: 272 3- 273 3 Lord R, Nair ... I interferon as a powerful adjuvant for monocyte-derived dendritic cell development and activity in vitro and in Hu-PBL-SCID mice Journal of Experimental Medicine 2000, 191: 177 7- 178 8 Ferrantini...
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Báo cáo sinh học: "Differential intracellular distribution of DNA complexed with polyethylenimine (PEI) and PEI-polyarginine PTD influences exogenous gene expression within live COS-7 cells" ppt
... labelled) and 10 μl of Lipofectamine 2000 (Invitrogen) was diluted in separate sterile microcentrifuge tubes, each containing 250 μl of incomplete DMEM (serum negative; penicillin/streptomycin negative; ... percentage of GFP positive cells treated with aphidicolin) It would be of interest to inhibit microtubule transport in post-mitotic cells to further define the role of microtubules in PEI trafficking ... to extend the current understanding of Arg internalization within live cells In addition, it is important to determine the fate of Arg peptides once internalized and, just as crucial, their potential...
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Báo cáo khóa học: Surface nucleolin participates in both the binding and endocytosis of lactoferrin in target cells potx
... Lf and HB-19 for binding to nucleolin, suggest that the C-terminal tail of nucleolin should be implicated in the mechanism of binding of Lf to nucleolin In general, the crosslinking of a ligand ... binding of hLf to CHO K1 (d), CHO 677 (j) and CHO 618 (r) cells (B) Scatchard analysis of the data showing two classes of hLf-binding sites on CHO K1 and CHO 677 cells in contrast to a single class ... the binding of HB-19 Similarly, the iron-binding hTf had no effect On the other hand, bLf inhibited the binding of HB-19 to cells In accordance with the involvement of both lobes of hLf in the...
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Báo cáo khoa học: Complex gangliosides are apically sorted in polarized MDCK cells and internalized by clathrin-independent endocytosis doc
... 153–158 Martina JA, Daniotti JL & Maccioni HJ (1998) In uence of N-glycosylation and N-glycan trimming on the activity and intracellular traffic of GD3 synthase J Biol Chem 273 , 372 5– 373 1 Paladino S, ... sphingolipids show differential partitioning into sphingolipid ⁄ cholesterol-rich domains in lipid bilayers Biophys J 79 , 1 478 –1489 6056 47 Mayor S & Pagano RE (20 07) Pathways of clathrin-independent ... However, in MDCK cells, we observed that the binding of CTx mainly occurs in the apical domains, whereas, in polarized CaCo-2 cells, the endocytosis of CTx is essentially the same at both apical and...
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Báo cáo khoa học: A novel pathway for sequential transformation of 7-dehydrocholesterol and expression of the P450scc system in mammalian skin pptx
... that 7- DHP is produced in the skin The level of 7- DHP production and its hypothetical conversion into other metabolites (including 17- , 20-, 21- and 11-hydroxy-7DHP) are the subject of investigations ... Center of Excellence in Connective Tissue (to A.S and J.Z.) and Center of Excellence in Genomics and Bioinformatics (to A.S.), UTHSC, and NIH grants 1R01-AR0 470 79-01A2 (to A.S.) and RR0 178 54 We ... 1B,C,E,F) In Fig 1C, in Ó FEBS 2004 4184 A Slominski et al (Eur J Biochem 271 ) Table Kinetic parameters for side-chain cleavage of 7- DHC and cholesterol by bovine and human cytochromes P450scc Kinetic...
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Báo cáo khoa học: Prohibitin is expressed in pancreatic b-cells and protects against oxidative and proapoptotic effects of ethanol pdf
... (nuclei) staining; (B) Mitofluor Red 589 (mitochondria) staining; (C) anti-PHB ⁄ anti-rabbit-FITC staining; (D) merge Arrows indicate staining of PHB D proteins have been shown to be involved in endocytosis ... diabetes and rat islet beta-cell damage induced by streptozotocin in vivo and in vitro Eur J Endocrinol 151, 277 –285 57 Ma Z, Zhang S, Turk J & Ramanadham S (2002) Stimulation of insulin secretion and ... mitochondrial function [44] and a reduced life span [45,46] Our findings in b -cells, which are in agreement with findings in yeasts, C elegans, and granulosa and intestinal cells, are in contradiction with...
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Báo cáo khoa học: Poneratoxin, a neurotoxin from ant venom Structure and expression in insect cells and construction of a bio-insecticide pot
... (1999) Interactions controlling the membrane binding of basic protein domains: phenylalanine 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 and the attachment of the myristoylated alanine-rich ... poneratoxin does not contain cysteine and it is conceivable that it has a distinct mode of action Classical arthropod toxins, such as those of scorpions, form a family of small proteins of 30 70 amino ... contained 75 nucleotides with an ATG codon in front of the gene and three stop codons at the end of it, and with flanking regions containing the restriction sites for BamHI and EcoRI The gene was inserted...
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Báo cáo khoa học: Differential regulation of fatty acid amide hydrolase promoter in human immune cells and neuronal cells by leptin and progesterone pdf
... leptin (Fig 1A) and progesterone (Fig 1B) increased FAAH protein in human U9 37 cells in parallel to the increase of enzymic activity RT-PCR amplification of cDNA of U9 37 cells showed a single band ... nonessential amino acids [18] Both CHP100 and U9 37 cells were maintained at 37 °C in humidified 5% CO2 atmosphere Incubation of U9 37 or CHP100 cells with leptin, alone or in the presence of different ... phospho-STAT3 increased only in U9 37 cells treated with 10 nM leptin (Fig 3A) In addition, nuclear levels of Ikaros isoforms Ik1, Ik2 and Ik3 were increased by treatment of U9 37 cells, but not of CHP100 cells, ...
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Báo cáo khoa học: "DEALING WITH INCOMPLETENESS OF LINGUISTIC KNOWLEDGE IN LANGUAGE TRANSLATION TRANSFER AND GENERATION STAGE OF MU MACHINE TRANSLATION PROJECT" potx
... Using Semantic Markers Word selection in the target language is a big problem in machine translation There are varieties of choices of translation for a word in the source language Main principles ... the generation process, the utilization of strong lexical information The system is in the course of development in collaboration with a number of computer scientists from computer industries and ... explained in 4.1 and 4.2 are involved at this generation stage The conversion is performed top-down from the root node of the dependency tree to the leaf Therefore when a governing verb demands...
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báo cáo hóa học: " Anandamide inhibits Theiler’s virus induced VCAM-1 in brain endothelial cells and reduces leukocyte transmigration in a model of blood brain barrier by activation of CB1 receptors" pdf
... enzymes (FAAH, MAGL) and proteins involved in their transport, and intracellular trafficking [9] Increasing evidence suggests the involvement of the ECS in both the inflammatory and the neurodegenerative ... side of injection, immunostained for VCAM-1 Arrows indicate VCAM-1 immunostaining Scale bar is 50 μm (B, D) Quantification of intensity of VCAM-1 staining as described in Material and methods in ... the role of AEA in regulating 1) VCAM-1 expression in brain endothelial cells infected Page of 13 with TMEV and the possible receptors involved by using antagonists of the classical cannabinoid...
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báo cáo hóa học:" Curcumin induces chemo/radio-sensitization in ovarian cancer cells and curcumin nanoparticles inhibit ovarian cancer cell growth" pot
... curcumin followed by cisplatin increased the percent of Annexin V positive cells and increased the amount of cleaved caspase and PARP, as compared to cisplatin or curcumin alone, indicating that ... curcumin pre-treatment increases the effectiveness of cisplatin treatment in cisplatin resistant cells by increasing the sensitivity of cells to apoptotic pathways and modulating nuclear β-catenin ... curcumin pre-treatment strategy and compared individual treatments (curcumin or cisplatin) to a combination of treatments (curcumin and cisplatin) (Figure 1A) When used individually, curcumin and...
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Báo cáo y học: "Targeting Toll-like receptor signaling in plasmacytoid dendritic cells and autoreactive B cells as a therapy for lupus" pps
... Interferon alpha/beta and interleukin 12 responses to viral infections: pathways 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 regulating dendritic cell cytokine expression in vivo J Exp ... TLR9-expressing cells) includes myeloid and pDCs, macrophages and MZ-B cells in mice, and pDCs, MZ-B cells and memory B cells in humans, which can respond directly and without priming to both single-stranded ... dendritic cells (mDCs) Activated T cells direct isotype switching and affinity maturation in autoreactive B cells dependent on CD40/CD40L interaction and IFN-γ secretion Independently of T cells, ...
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Báo cáo y học: "Expression of the inflammatory chemokines CCL5, CCL3 and CXCL10 in juvenile idiopathic arthritis, and demonstration of CCL5 production by an atypical subset of CD8+ T cells" pps
... protein (Figure 6) In addition, staining was demonstrated at the synovial lining and on many infiltrating inflammatory cells Protein levels of inflammatory chemokines in synovial fluid Protein ... Kidlington, Oxford, UK) and anti-CCR7 (R&D Systems) For intracellular staining of CCL5 chemokine, cells were fixed in 4% paraformaldehyde (Sigma) in phosphate-buffered saline and permeabilised in ... to our understanding of recruitment of T cells to the joint in inflammatory arthritis and suggests that in the microenvironment of the joint, dysregulation of functional patterns of expression...
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Báo cáo khoa học: "The Akt-inhibitor Erufosine induces apoptotic cell death in prostate cancer cells and increases the short term effects of ionizing radiation" pot
... to induce apoptosis in tumor cells and their ability to increase cytotoxic efficacy of chemotherapy and radiotherapy in preclinical investigations [10-12] Induction of apoptosis by ErPC3 and ... phosphorylation of Akt only in the LY2 9400 2-sensitive LNCaP cells but not in PC3 cells with low sensitivity to LY2 9400 2 Thus, the antineoplastic activity of ErPC3 and LY2 9400 2 in prostate cancer cells ... potency In contrast, the anti-neoplastic effects of ionizing radiation in LNCaP cells did not involve apoptosis induction implicating a role of proliferation inhibition or the induction of non-apoptotic...
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Báo cáo khoa học: "The interaction between different types of activated RAW 264.7 cells and macrophage inflammatory protein-1 alpha" potx
... production of interleukin-4 during radiation-induced pneumonitis and pulmonary fibrosis in rats: macrophages as a prominent source of interleukin-4 Am J Respir Cell Mol Biol 19 97, 17: 315-325 Modolell ... Burdick MD, Lincoln P, Evanoff H, Kunkel SL: Production and function of murine macrophage inflammatory protein-1 alpha in bleomycin-induced lung injury J Immunol 1994, 153: 470 4-12 Standiford TJ, ... McBride WH, and Chiang CS: Bronchoalveolar lavage and interstitial cells have different roles in radiation-induced lung injury Int J Radiat Biol 2003, 79 :159-1 67 doi:10.1186/ 174 8 -71 7X-6-86 Cite...
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Báo cáo khoa học: "Impaired antiviral activity of interferon alpha against hepatitis C virus 2a in Huh-7 cells with a defective Jak-Stat pathway" pdf
... GCTCGTCCAT-3’) In the third step, the recombinant 531 bp DNA fragment (F3) containing last amino acids of EGFP-N1 and 177 amino acids of NS5A (nt 75 478 077 ) was amplified by using sense primers (S /75 47/ HCV- ... with alpha interferon in S-Huh -7 cells (B) Intracellular GFP expression in the infected R-Huh -7 cells in culture at 24, 48, 72 and 96 hours of postinfection in the presence and absence of IFN-a ... IFN-a induced ISRE-luciferase activity in S-Huh -7 and R-Huh -7 cells after HCV 2a infection Both S-Huh -7 and R-Huh -7 cells were were infected with full-length HCV After 72 hours, infected cells...
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Báo cáo y học: "A crucial role for tumor necrosis factor receptor 1 in synovial lining cells and the reticuloendothelial system in mediating experimental arthritis" pps
... organs, such as liver and spleen, are rich in mature and functional cells of Page of 11 Figure Effects of silencing tumor necrosis factor receptor (TNFR1) in synovial lining cells during streptococcal ... requirements by CD4+ and CD8+ T cells for soluble and membrane TNF in control of Francisella tularensis live vaccine strain intramacrophage growth J Immunol 20 07, 179 :77 09 -77 19 Muller S, Rihs S, Schneider ... activation of macrophages [38,39] TNFR1 silencing in SLCs resulted in a significant reduction of secreted IL-6 and IL-1β levels in the joint, which indicates an inhibition of the local cytokine cascade...
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Báo cáo y học: " Nontypeable Haemophilus influenzae induces COX-2 and PGE2 expression in lung epithelial cells via activation of p38 MAPK and NF-kappa B" pps
... TLR2 in epithelial cells in vitro and lung tissues of mice in vivo COX-2 up-regulation mediated by TLR2 in epithelial cells in vitro and lung tissues of mice in vivo A HEK293 cells overexpressing ... COX-2 expression in vivo, wild type, and TLR2 and TLR4 knock-out mice were intratracheally inoculated with × 1 07 CFU of NTHi strain 12, and mRNA expression of COX-2 in lungs of infected mice were ... signaling pathway in lung epithelial cells, respectively, we investigated the possible involvement of TLR2 and TLR4 in NTHi-induced COX-2 expression in vitro by using HEK293 cells overexpressing...
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Báo cáo y học: " Synergistic effect of human CycT1 and CRM1 on HIV-1 propagation in rat T cells and macrophages" docx
... percentage of living cells was approximately 20 40% , and approximately 75 % of the living cells were GFP+ The amount of p24 in the sample containing hCRM1-HA was 196 pg Approximately 50 μg samples of ... strains of HIV-1 The efficiency of the early steps of infection, including reverse transcription, nuclear import, and integration in macrophages and T cells of Sprague-Dawley rats is comparable ... http://www.retrovirology.com/content/6/1/43 Figure Infectivity of HIV-1 produced in rat and human cells Infectivity of HIV-1 produced in rat and human cells The medium [containing 50 or 500 pg of p24] from the various...
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Tìm thêm: xác định các mục tiêu của chương trình xác định các nguyên tắc biên soạn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản khảo sát chương trình đào tạo gắn với các giáo trình cụ thể xác định thời lượng học về mặt lí thuyết và thực tế tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra đối với đối tượng giảng viên và đối tượng quản lí điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct đặc tuyến hiệu suất h fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến tốc độ rôto n fi p2 đặc tuyến dòng điện stato i1 fi p2 sự cần thiết phải đầu tư xây dựng nhà máy thông tin liên lạc và các dịch vụ phần 3 giới thiệu nguyên liệu từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008 chỉ tiêu chất lượng 9 tr 25