protocols of in vitro protein covalent binding studies in liver
Báo cáo khoa học: In vitro embryonic developmental phosphorylation of the cellular nucleic acid binding protein by cAMP-dependent protein kinase, and its relevance for biochemical activities pdf
... characterization of a JC virus pentanucleotide repeat element binding protein: cellular nucleic acid binding protein Virus Res 58, 73–82 Flink IL & Morkin E (1995) Alternatively processed isoforms of cellular ... properties of a variety of proteins with common biochemical activities, such as the binding to single-stranded nucleic acids and the remodelling of their secondary structures A model involving CNBP ... Regardless of the evaluated CNBP form or the nature of the analysed probe, band-shift intensity increased along with the protein increasing amount Nevertheless, no significant differences in binding...
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Tài liệu Báo cáo khoa học: Role of K22 and R120 in the covalent binding of the antibiotic fosfomycin and the substrate-induced conformational change in UDP-N-acetylglucosamine enol pyruvyl transferase docx
... mutant protein, but is calorimetrically silent with the K22V and K22E mutant proteins This was interpreted as a lack of fosfomycin binding to these mutant proteins [12] Binding of fosfomycin to ... enthalpies of binding and stoichiometries were determined from the binding isotherm by fitting a : binding model to the data using the software provided by the manufacturer Results Reaction of fosfomycin ... chain of K22 participates in fosfomycin binding, thus providing a rationale for the loss of fosfomycin binding to the K22V and K22E mutant proteins However, two other positively charged amino...
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Báo cáo khoa học: Studies on the role of the receptor protein motifs possibly involved in electrostatic interactions on the dopamine D1 and D2 receptor oligomerization pdf
... physical interactions between receptor proteins [51,52] Results Radioligand binding assay As shown in Table 1, the binding parameters obtained for dopamine D1 receptor and its mutant indicate ... role of certain amino acid residues in the formation of noncovalent complexes between protein molecules was highlighted Electrostatic interactions occur between an epitope containing mainly two ... its receptor trafficking properties The binding experiments also pointed to a decrease in the density of the D2R variants in the cellular membrane; the number of D2 receptor binding sites decreased...
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Báo cáo khoa học: ATP-binding domain of heat shock protein 70 is essential for its effects on the inhibition of the release of the second mitochondria-derived activator of caspase and apoptosis in C2C12 cells potx
... interaction of HSP70 with Smac but requires the ATP -binding domain of the protein However, it ATP -binding domain of HSP70 inhibits Smac release remains to be determined how these findings are connected ... ATP -binding domain of HSP70 is critical for sequestering AIF in the cytosol [29] In the present study, we demonstrated that the ATP -binding domain of HSP70 was indispensable for inhibition of ... Jiang et al The ATP -binding domain of HSP70 is important for the interaction of HSP70 with apoptosis signalregulating kinase (ASK1) and the inhibition of ASK1-induced apoptosis in vitro [28] Furthermore,...
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Báo cáo khoa học: Role of CCP2 of the C4b-binding protein b-chain in protein S binding evaluated by mutagenesis and monoclonal antibodies docx
... b-chain in immobilized C4BP In uence of protein S binding of monoclonal antibodies To investigate whether protein S and MoAb 15/ MoAb 44 had overlapping binding sites on the b chain, the following ... sterical hindrance between protein S and MoAb 44 Discussion The binding site for protein S on C4BP is predominantly contained in CCP1 of the b-chain [9] A key binding surface for protein S involves ... The binding of both MoAb 15 and MoAb 44 to the b-chain was Ó FEBS 2003 The role of CCP2 of the C4BP b-chain in binding of protein S (Eur J Biochem 270) 97 Fig SDS/PAGE analysis of recombinant...
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Báo cáo khoa học: The calcium-binding domain of the stress protein SEP53 is required for survival in response to deoxycholic acid-mediated injury pdf
... damage in vitro Cellular stress protein responses play a key role in minimizing cell injury and maintaining tissue integrity in response to damaging levels of an environmental agent As such, the integrity ... SEP53 protein, (C) SEP70 protein, (D) AG-2 protein and (E) loading controls for squamous and Barrett’s biopsies (ink stain immunoblot to normalize for protein loading), as indicated (F) Immunostaining ... plated in media containing Geneticin to select for cell containing vector DNA (as in Fig 5A,G) After three weeks, the number of cells was determined by fixing cells and staining with dye Individual...
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Báo cáo Y học: Identification and characterization of a novel activated RhoB binding protein containing a PDZ domain whose expression is specifically modulated in thyroid cells by cAMP pot
... existence of a Rho -binding domain (HR-1) in the amino terminal part of the protein suggests an implication in transduction pathways involving the Rho proteins By use of the two-hybrid system and of in ... the PDZ domains existing in a wide variety of proteins The protein ends by a potential PDZ binding domain motif (SSWY) and contains at least two potential phosphorylation sites (indicated by ... form of RhoB The presence of an HR1 domain and the high degree of homology with Rhophilin in the NH2 part of the protein suggested that p76RBE could be able to interact with a small G protein of...
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Báo cáo y học: "Analysis of C4 and the C4 binding protein in the MRL/lpr mouse" pptx
... Trapp RG: Free protein S levels are elevated in familial C4b -binding protein deficiency Blood 1990, 76:2527-2529 Hessing M, van TVC, Bouma BN: The binding site of human C4b -binding protein on complement ... control protein domains Human C4bp has been shown to bind other compounds including protein S (β chain), C-reactive protein, serum amyloid protein, soluble CD40 ligand, CD40 (α chain), heparin (α ... protein: targeting of the inhibitory activity of C4b -binding protein J Immunol 2006, 176:7612-7620 Webb JH, Blom AM, Dahlback B: The binding of protein S and the protein S-C4BP complex to neutrophils...
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Báo cáo y học: "Reverse genetic characterization of the natural genomic deletion in SARS-Coronavirus strain Frankfurt-1 open reading frame 7b reveals an attenuating function of the 7b protein in-vitro and in-vivo" pptx
... persist in their reservoirs without causing disease The characterization of virus proteins can be unreliable if only the protein of interest is studied on its own The study of proteins in the ... deletion in ORF 7b as shown in Figure (B) Plaque assay using crystal violet stain and immunofocus assay using a polyclonal protein patient serum reacting predominantly against the N protein (anti-N) ... C-terminus of ORF 7b As shown in Figure 6A, a protein band corresponding to the predicted molecular weight of the 7b protein (5.3 kDa) was specifically detected in rSCV7bflaginfected cells using...
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Báo cáo khoa học: " Reverse genetic characterization of the natural genomic deletion in SARS-Coronavirus strain Frankfurt-1 open reading frame 7b reveals an attenuating function of the 7b protein in-vitro and in-vivo" docx
... persist in their reservoirs without causing disease The characterization of virus proteins can be unreliable if only the protein of interest is studied on its own The study of proteins in the ... deletion in ORF 7b as shown in Figure (B) Plaque assay using crystal violet stain and immunofocus assay using a polyclonal protein patient serum reacting predominantly against the N protein (anti-N) ... C-terminus of ORF 7b As shown in Figure 6A, a protein band corresponding to the predicted molecular weight of the 7b protein (5.3 kDa) was specifically detected in rSCV7bflaginfected cells using...
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Báo cáo khoa học: "Application of a zona pellucida binding assay (ZBA) in the domestic cat benefits from the use of in vitro matured oocytes" pptx
... decreased binding to FT ZP In line with this argumentation, such decreased binding capacity of the ZP may reside in changes of the structure of the ZP that occur during unprotected freezing and/ ... in viability between fresh and FT spermatozoa may explain the differences in ZP binding registered However, the lack of binding to FT ZP is mainly due to the ZP and not to the spermatozoa, since ... spermatozoa bound to the ZP of in vitro matured oocytes (Table 1, Figure 1) The percentage of binding of electroejaculated spermatozoa was higher in in vitro matured ZP than in the immature, FT ZP...
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Báo cáo y học: "A comparison of high-mobility group-box 1 protein, lipopolysaccharide-binding protein and procalcitonin in severe community-acquired infections and bacteraemia: a prospective study" ppt
... lipopolysaccharide -binding protein, interleukin-6 and C-reactive protein in community-acquired infections and sepsis: a prospective study Crit Care 2006, 10:R53 39 Rintala EM, Aittoniemi J, Laine S, Nevalainen ... JR: The role of lipopolysaccharide -binding protein in modulating the innate immune response Microbes Infect 2006, 8:946-952 Gendrel D, Raymond J, Coste J, Moulin F, Lorrot M, Guộrin S, Ravilly ... abovementioned findings in previous studies Diagnosis of bacteraemia at the present time is a relatively slow process, requiring up to several days of culturing/processing in the laboratory of microbiology...
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Báo cáo y học: " Role of Dietary Soy Protein in Obesity"
... IgG4 -binding proteins in soybean by immunoblotting with sera from 30 soybean-sensitive patients [88] Ten proteins were detected as IgE -binding proteins and proteins as IgG4 -binding proteins, ... been identified in a number of studies in soybean-sensitive patients These include include b-conglycinin, glycinin, soy vacuolar protein, Kunitz trypsin inhibitor, and other proteins [83,87-89] ... amino acid score of 1.0, which is the same as that of casein and egg protein [28] However, soy proteins contain low methionine/glycine and lysine/arginine ratios compared to casein [29] Soy protein...
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Tài liệu A REVIEW OF INDOOR AIR POLLUTION AND HEALTH PROBLEMS FROM THE VIEWPOINT OF ENVIRONMENTAL HYGIENE: FOCUSING ON THE STUDIES OF INDOOR AIR ENVIRONMENT IN JAPAN COMPARED TO THOSE OF FOREIGN COUNTRIES pptx
... phthalates were detected predominantly in indoor air samples.93) The dominant path of phthalates intake was the ingestion of foodstuffs compared to inhalation of indoor air by children.94) n-Tetradecane ... can meet local needs in dealing with SHS.152) CONCLUSION Healthy indoor air is a fundamental right for people studying in schools, working in of ces and living in residences Indoor air quality ... combustion gases in residences and buildings CO is well-known to cause poisoning by CO-hemoglobin (Hb) formation, inhibiting oxygen utilization by internal organs NO2 sources in buildings include gas...
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Tài liệu Báo cáo khoa học: Evolutionary divergence of valosin-containing protein ⁄cell division cycle protein 48 binding interactions among endoplasmic reticulum-associated degradation proteins ppt
... neighbouring amino acids also in uence binding, and a similar motif required for VCP binding in gp78 tolerates substitution of these two arginines We map the site of binding of Ube4b to the N-domain of ... EDEEE(192–196) of VCP is required for binding Individual point mutations in this VCP sequence did not alter binding of WldS (Fig 4B) Mutually exclusive binding of VBM-containing proteins to VCP As ... identified arginine-rich motifs in three further ubiquitinmetabolizing proteins, the zinc finger deubiquitinating protein OUT domain-containing protein (OTUD7a) [29], and the ligases Smad ubiquitination...
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Tài liệu Báo cáo khoa học: Verprolin function in endocytosis and actin organization Roles of the Las17p (yeast WASP)-binding domain and a novel C-terminal actin-binding domain doc
... VH2-N) which we previously showed does contain an actinbinding domain (this actin -binding domain has not yet been mapped) [23] We name the actin -binding domain that we have identified VH2-C and VH2-N ... Vrp1p domain structure Schematic of Vrp1p showing the Vrp1p truncations and mutant proteins used in this study and their various known domains: actin -binding domains, Hof one trap (HOT) domain, and ... cluster interacts with G-actin, but not F-actin An alignment of the various known and putative actin -binding sequences in Vrp1p is shown in Fig 4A Vrp1p-WH2-1 is the WH2 domain at the N-terminus of...
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Tài liệu Báo cáo khoa học: Crystal structure of the BcZBP, a zinc-binding protein from Bacillus cereus doc
... determination of the BcZBP ˚ protein at a resolution of 1.8 A, provides important clues towards understanding the enzyme function, including the identification of a zinc -binding site within a potential ... the amino acid level The protein encoded by bc1534 is classified as a LmbE-related protein and hereafter will be referred to as BcZBP (B cereus zinc -binding protein) The N-terminal part of BcZBP ... 15 Chothia C (1975) Structural invariants in protein folding Nature 254, 304–308 16 Eisenberg D & McLachlan D (1986) Solvation energy in protein folding and binding Nature 319, 199–203 17 Fadouloglou...
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Tài liệu Báo cáo khoa học: Identification of membrane-bound serine proteinase matriptase as processing enzyme of insulin-like growth factor binding protein-related protein-1 (IGFBP-rP1/angiomodulin/mac25) doc
... level of proteinase(s) responsible for the processing of IGFBP-rP1 To determine the class of the IGFBP-rP1-cleaving proteinase, OVISE cells were cultured in the presence of various proteinase inhibitors ... the production of the cleaved form of IGFBP-rP1 (25 kDa), whereas a cysteine proteinase inhibitor (leupeptin), an aspartic proteinase inhibitor (pepstatin) and a metalloproteinase inhibitor (N-(R)(2-(hydroxyaminocarbonyl)methyl)-4-methylpentanoyl-l3-(2¢-naphthyl)alaninyl-l-alanine ... in kDa Analysis of serine proteinases secreted by various cell lines Trypsin-type serine proteinases present in the conditioned media of the nine cell lines were analyzed by gelatin zymography...
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Tài liệu Báo cáo khoa học: The role of the ESSS protein in the assembly of a functional and stable mammalian mitochondrial complex I (NADH-ubiquinone oxidoreductase) pptx
... domain extending into the intermembrane space In the third mutant (V79-G35) one might expect a protein to be inserted into the inner membrane, but it is missing a major portion of the domain localized ... protein is not assembled into the human complex I Inspection of the amino acid sequences of the known mammalian ESSS proteins reveals a high degree of conservation in the C-terminal domain (including ... (including the transmembrane region), but a significant number of differences in the N-terminal domain (located on the matrix side) It is likely that the N-terminal domain is involved in protein protein...
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Tài liệu Báo cáo khoa học: Covalent binding to glutathione of the DNA-alkylating antitumor agent, S23906-1 doc
... Dudones, L.P & Bose, R.N (2002) HPLC determination of binding of cisplatin to DNA in the presence of biological thiols: implications of dominant platinum-thiol binding to its anticancer action Pharm ... as, in the present case, the GSH–benzoacronycine adducts reduce the cytotoxic action of the drug It should be included in the second group of compounds for which the covalent binding to GSH inactivates ... cytotoxic action of this DNA minor-groove -binding agent is significantly enhanced in the presence of GSH and this effect is believed to originate from the formation of GSH–brostallicin covalent adducts...
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Tìm thêm: xác định các mục tiêu của chương trình khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam nội dung cụ thể cho từng kĩ năng ở từng cấp độ xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ lồng sóc mở máy động cơ rôto dây quấn các đặc tính của động cơ điện không đồng bộ đặc tuyến mômen quay m fi p2 đặc tuyến tốc độ rôto n fi p2 đặc tuyến dòng điện stato i1 fi p2 sự cần thiết phải đầu tư xây dựng nhà máy thông tin liên lạc và các dịch vụ phần 3 giới thiệu nguyên liệu từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008