0

protein enzyme structure function and degradation

Identifying protein co factors of oct4, an essential stemness transcription factor, by affinity purification and mass spectrometry

Identifying protein co factors of oct4, an essential stemness transcription factor, by affinity purification and mass spectrometry

Cao đẳng - Đại học

... Ionization Multi-Dimensional Protein Identification Technology N-terminal BAP and His tag N-terminal domain N-terminal FLAG and His tag N-terminal Protein A and CBP tag N-terminal S and CBP tag Reversed-phase ... approach The expression of proteins in E.coli and use of large expression tags like GST and maltose binding protein (MBP) may not be suitable for all eukaryotic proteins, and causes misfolding that ... use of IP and Co-IP is widely accepted as a robust means of demonstrating an actual protein- protein interaction, and is typically assessed by the specific detection of the protein target and bait...
  • 209
  • 293
  • 0
Application of membrane technologies for biomolecules separation and purification by affinity binding to specific ligands

Application of membrane technologies for biomolecules separation and purification by affinity binding to specific ligands

Tổng hợp

... (A) and near-UV CD spectra (B) for native, one time acid-denatured and one time base-renatured HSA Figure 3.6 Comparison of yields of L-tryptophan (A) and D-tryptophan (B) between native (●) and ... short testing time and high selectivity and sensitivity are compulsory In this regard, the chromatography and electrophoresis techniques are able to suffice such requirements and commonly selected ... f ,L where Cp,D and Cp,L are the concentrations of D-enantiomer and L-enantiomer in the permeate side, respectively, while Cf,D and Cf,L are the concentrations of D-enantiomer and L-enantiomer...
  • 56
  • 258
  • 0
ELECTROSPUN AFFINITY MEMBRANE FOR IMMUNOGLOBULIN g PURIFICATION

ELECTROSPUN AFFINITY MEMBRANE FOR IMMUNOGLOBULIN g PURIFICATION

Tổng hợp

... both Protein A and Protein G It is a gene fusion product secreted from a nonpathogenic form of Bacillus The secreted Protein A/G contains four Fc-binding domains from Protein A and two from Protein ... towards designing and selecting ligands of high affinity and specificity [Figure 2.9] Ligands for protein affinity chromatography are distinguished into synthetic and biological The ligand-specific ... eliminated Protein A/G binds to all human IgG subclasses and it has a broader binding range than either Protein A or Protein G individually Although IgG purification kits utilizing protein A or protein...
  • 127
  • 277
  • 0
bài tập lớn.doc

bài tập lớn.doc

Công nghệ thông tin

... int0 org 0013h ljmp int1 ; - MAIN PROGRAM org 30h main: MOV IE,#00000000B ; Enable int1 and int mov p2,#00h CLR A MOV MOV MOV MOV MOV l: A,#0ffh p2,A A,#066h B,#066h 7fh,#00h MOV R4,#077h ... int0 org 0013h ljmp int1 ; - MAIN PROGRAM org 30h main: MOV IE,#10000101B ; Enable int1 and int mov p2,#00h CLR A MOV A,#0ffh MOV p2,A MOV A,#066h MOV B,#066h MOV 7fh,#00h l: ACALL Forward...
  • 22
  • 2,493
  • 3

Xem thêm