... present for the last four residues at the C-terminus. The stereochemistry ofthe model hasbeen assessed using the program procheck [23]. Ninetyper cent ofthe residues fall in the most favouredregions ... metal-bound hydroxyl group onto the substrate, as observedin other members ofthe amidohydrolase superfamily[24,25]. However, as no structureof complexes witheither the substrate, product or inhibitors ... content of 50%. Data were pro-cessed using the program mosflm [38], and the ccp4 suite of programs [39] was used for scaling. Structure deter-mination for hACMSD was performed by means of the...
... to the enzyme. This may indicate that the steric limitation of the enzyme’s binding pocket takes place in the areabetween the ortho and para positions ofthe aromaticsubstrate.On the basis of ... removes the steric hindrance and allows the approach of 2IPA towards the catalytic iron.Residue I248 lies at the entrance ofthe substrate-binding pocket ofthe enzyme, leading to the substratechannel. ... [14]. The lack of characterization of the structural determinant ofthe substrate specificity of the AtdA enzyme has thus limited its development as abiocatalyst for the bioremediation of a wide...
... A˚resolution bythe molecular replacement method. The schematic view ofthe overall structure is shown in Fig. 3A.Theenzymeexistsasadimeroftheabc heterotrimer. Thereis a noncrystallographic twofold ... cob(II)alamin by X-rayirradiation [59]. Therefore, we believe that the structure reported in this paper is also that ofthe glyceroldehydrataseÆcob(II)alamin complex. The dihedral angle of the northern ... from a bulk of water by the corrin ring of cobalamin. Figure 4B shows the structure around the enzyme-bound cobalamin. The cobalaminmolecule is bound between the a and b subunits in the so-called...
... [27]. On the basis of the Ping Pong mechanism, the acetylation rate (k2 )of NAT2 bythe acetyl donor is independent ofthe trans-acetylation rate (k4) ofthe arylamine substrate.Because the values ... the active site. The more dras-tic mutations, Y190I and Y190A, however, revealed the importance ofthe phenyl ring ofthe Tyr sidechain on the pH dependence of enzyme acetylation.Both ofthe ... trunca-tions, the availability ofthe Tyr190 mutants makes itfeasible to conduct cell-based studies ofthe effects of the stability ofthe acetylated enzyme intermediate on the N-acetylation of aromatic...
... HeLa?Chapter Ten: The Other Side ofthe Tracks1. Explain the meaning ofthe idiom the other side ofthe tracks.” 2. What do the names ofthe creek and the river suggest about life in Lacks Town?3. How ... in the development ofthe field of cell culture, and the industry of selling HeLa cells and other human biologicalmaterials.11. Who profited monetarily from the sale of HeLa cells and other ... her motherand her mother’s cells. The result of their relationship is an illuminating portrait of the enduring legacy of Henrietta’s life, death, and immortality. about the bookabout the authorrandom...
... the form curl/π(2)+ E(2).5. Strategy ofthe proof ofthe Asymptotics TheoremIn this section we describe the main ideas in the proof ofthe AsymptoticsTheorem.(1) Section 6. We start by ... given a detailed proof ofthe theorem by relying heav-ily on a result, which we have called the Asymptotics Theorem, concerning the geometric properties ofthe causal structureof appropriately ... I+0is the future domain ofthe originon Σ0.Remark 9.3. The proof of Corollary 9.2 requires an extension argument. The estimates ofthe Asymptotics Theorem, which are uniform with respectto the...
... bestIM:Type full timeIM:Title the godfatherOther the, in, that Other the, in, that Other the, in, thatTable 3: Label sets and their respective query segment examples for the intent class Movie, Job ... goal of this work is to identify the semantic labels of IMs,i.e., the attribute names they implicitly refer to. Weuse Acto denote the set of IM semantic labels for the intent class c.OtherAdditionally, ... name of an intent class.For example, the IH ofthe query “alice in won-derland 2010 cast” is “cast”, which is an attributename of Movie. By issuing the query, the user in-tends to find out the...
... A˚,respectively, for the backbone ofthe two subunits. The difference between rmsd of monomeric and dimeric speciesis due to the indetermination ofthe reciprocal orientation of the two subunits. The average ... was used to back calculate the NOESYcross-peaks from the calculated structure to assess the quality ofthe structure. The final family was made up of 30 structures with the lowest target function. ... is c onsidered. The c omparison of the present structure with the X -ray structures ofthe humanoxidized protein ( 1SOS) [11] and its G 37R mutant [50]show that the protein has the same folding...
... to the active site. To dissect the structure function relationships ofthe LPL carboxyl-terminal domain, several residues predicted bythe model structure to be essential for the functions of ... modeling ofthe dimeric structureof LPL. Two dimeric model structures of LPL were constructed usingINSIGHT IIversion 2000 ona Silicon Graphics workstation. The model structureofthe closed ... on the crystal structures ofthe human, porcine, and horse PLs(Fig. 1). A frontal view illustrates the overall saddle shape of the dimeric structure and the key heparin-binding residuesin the...
... secure the services of eminent members of appropriate professions in the examination of policy matters pertaining to the health ofthe public. The Institute acts under the responsibility given to the ... 20418NOTICE: The project that is the subject of this report was approved by the Governing Board ofthe National Research Council, whose members are drawnfrom the councils ofthe National Academy of Sciences, ... recognizes the superiorachievements of engineers. Dr. Wm. A. Wulf is president ofthe National Academy of Engineering. The Institute of Medicine was established in 1970 bythe National Academy of Sciencesto...
... reduced level of activityDA and other catecholamines have a stabilizing effecton the conformation of TH [33]. We therefore com-pared the thermostability of hTH at 37 °C in the pres-ence of NMNorsal, ... ligation between the two hydroxyl residues of their catechol moiety and the iron. The unbound ends of these molecules projected from the binding pocket.Potentially, they interact with the enzyme’s ... presence ofthe non-hydroxylatedNMTIQ accounted for less than 4% of the total iron. **P < 0.01 compared to DA orany ofthe other TIQs in a one-wayANOVAfollowed by Tukey’s test. (C) Activity of hTH...
... ª 2004 FEBS the full-length HOP, they act to facilitate the assembly of multichaperone regulatory complexes. The struc-tural independence of these TPR domains, and the presence of independent ... First, the only structures of natural TPRs in the PDB (13 TPR-containing coordi-nate sets) consist of nonglobular, extended arrays of helices [9,10,13–21]. Second, the majority of these(11 structures) ... TPR1 and TPR2A of ABDCFig. 2. Solubility of NlpI constructs and thestructureof mature NlpI. (A) 10–20% gradient SDS ⁄ PAGE of NlpI expression products, showing the insolubility ofthe 3-TPR construct...
... another than to any other profile in the data set. The profiles of G can be summarized by a vector s whose dimensionality is that ofthe data, and each of whose elements contains the mean of ... disparity in the frequency of its occurrence in A and B is the first significant finding ofthe proposed methodology. The scaling ofthe ‘Allah’ variable dominates all the other variables. ... understanding their thematic interrelationships the object of this exercise—but it is not sufficient because it provides no information about the thematic characteristics ofthe clusters and the thematic...
... orientation.Accordingly, the present data on the molecular mass of the native Hb complex as well as the Hb subunitsare inconsistent. In addition, there are only two hypo-thetical models concerning thestructureof ... identification ofthe carbohydrates bound toD. magna Hb subunits, saccharides were released by methanolysis and separated by GC followed by MS.Chromatograms ofthe carbohydrates of D. magna Hb, of the ... of this interesting molecule. The present study has contributed to this approach:(a) the determination ofthe molecular mass of the native Hb complex and its subunits as well as the number of...
... reduceoutput by restricting the use ofthe property, and if so this is as much a cost of the property right as the cost ofthe fence is. Suppose the owner of a shop-ping center does charge for the use of ... for the original. In the case of books and other printed matter, the copier may not beable to match the quality of paper or binding ofthe original or the crispness of the printing, and there ... numerous, the trans-action costs of their buying the right to use the river from the factory may ex-ceed the value ofthe right to them. In such a case a liability rule would bebetter, whereby the...