poly i c to the lungs of mice
Báo cáo y học: " Lymphocyte apoptosis in murine Pneumocystis pneumonia Xin Shi, Nicole J LeCapitaine, Xiaowen L Rudner, Sanbao " potx
... 5'-AAACTTACACAAGGAGGGTGTTTG-3', 5'-AATGCCTTCTCCATACCAGACG-3', 5'-TTACCGCGAGGCTGAAGACCACCC-3'; mSurvivin, 5'-ATCGCCACCTTCAAGAACTGG-3', 5'TCAGGCTCGTTCTCGGTAGG-3', 5'-ATGAAGCCAGCCT CCGCCATTCGC-3'; ... infected Scid mice [24] In brief, Scid mice chronically infected with Pneumocystis were injected with a lethal dose of pentobarbital The animals were then exsanguinated by abdominal aortic transaction ... pre-infection CD4 or CD8 cells pathway is initiated by ligation of cell surface "death receptors" culminating in activation of initiator caspase-8 and cell death [38,39] The results of the current...
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Báo cáo khoa học: "Density of CD4(+) and CD8(+) T lymphocytes in biopsy samples can be a predictor of pathological response to chemoradiotherapy (CRT) for rectal cancer" ppt
... shown in Figure 2, when the cases were divided by the histological response grade according to the definitions in the Japanese Classification of Colorectal Carcinoma, the density of CD4(+) T cells ... [16,22,23] Since anal carcinoma is usually associated with human papilloma virus, specific viral proteins processed in tumor cells may critically affect the histological characteristics of the tumor ... (Novocastra, CA) The specificities of these mAbs in immunohistochemistry on paraffin embedded samples were confirmed with human tonsil tissue sections (data not Figure Immunohistochemical detection...
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Báo cáo hóa học: " Decreased level of recent thymic emigrants in CD4+ and CD8+T cells from CML patients" pdf
... Orlanducci F, Dietrich PY, Gaudin C, Angevin E, Courtier MH, Bayle C, Hercend T, Triebel F: T cell repertoire in patients with B chronic lymphocytic leukemia Evidence for multiple in vivo T cell clonal ... explain the significant reduction of recent thymic emigrant numbers in peripheral blood of CML patients, as measured by quantitative detection of δRecψJα sjTRECs In conclusion, this is, to our ... prominent decrease of sjTRECs levels in CML, indicating the reduction of recent thymic emigrants affects the majority of TRBV subfamilies Page of Competing interests The authors declare that they...
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Báo cáo y học: "Heterogeneity of CD4+ and CD8+ memory T cells in localized and generalized Wegener’s granulomatosis." potx
... to chemotactic gradients, cytokine release and cytotoxic activity of distinct T cell populations Detection of the inducible inflammatory chemokine receptors CCR5 and CCR3 on CD45RA+ T cells suggests ... Thus, the local cytokine milieu might depend on the site and extent of disease activity [4,5] The shift in the cytokine profile in granulomatous lesions of the respiratory tract might be of importance ... 3), i. e evidence of partial or complete improvement of vasculitis activity by clinical and serological investigations and by imaging procedures All patients with localized WG were in remission,...
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Báo cáo khoa hoc:" Quantitative competitive reverse transcription polymerase chain reaction is not a useful method for quantification of CD4 and CD8 cell status during HIV infection" pptx
... immunodeficiency virus infection and diagnostic and prognostic application to infected children Pediatr Infect Dis J 1992, 11(8):639-644 Publish with Bio Med Central and every scientist can read ... with NIH image) on the x-axis versus the log of incremental dilutions of competitor DNA on the y-axis Solving for the antilog of x when y = will give the concentration of unknown amount of DNA ... ethidium bromide can be quantitative provided that digital imaging is employed [9] The standard curve plots the log cDNA/competitor band intensity (as determined by analysis of PICT files with...
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Báo cáo y học: "Genome-wide analysis of primary CD4+ and CD8+ T cell transcriptomes shows evidence for a network of enriched pathways associated with HIV disease" pot
... transcriptomic level, which will facilitate the understanding of the genetic basis of transcriptomic interaction of HIV in vivo and how HIV subverts the human gene machinery at the individual cell ... closely articulated with TCA cycle and OXPHOS pathways pointing towards mitochondrial dysfunction The significance of mitochondrial dysfunction is further strengthened by the detection of coordinately ... accompanying mitochondrial dysfunction were identified [36], of which 15 (> 50%) were shared with our list Pathways involved in mitochondria-mediated cell apoptosis The significance of mitochondrial...
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Báo cáo y học: " Antibody microarray analysis of cell surface antigens on CD4+ and CD8+ T cells from HIV+ individuals correlates with disease stages" ppt
... that the defective expression of CD56 on these cells in HIV-infected individuals could contribute to the decreased peripheral blood T-cell cytotoxicity found in HIV infection [41] These findings, ... individual variability in antigen expression significantly increased expression For HIV-specific CD8+ cells, diminished proliferative capacity results in a memory T cell population with reduced capacity ... study is the first to relate one of the components of CD3 complex, CD3epsilon, to HIV disease status, yet the biological significance of CD3epsilon expression in HIV disease requires further elucidation...
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Báo cáo y học: "Isolated receptor binding domains of HTLV-1 and HTLV-2 envelopes bind Glut-1 on activated CD4+ and CD8+ T cells" pot
... and MC participated in the design and validation of antibodies and receptor binding domain constructs as well as binding studies; NM helped to conceive the studies, participated in their design ... envelope This effect was specifically due to the action of CytB on Glut-1 and not to an indirect cytochalasin effect on microfilaments: Treatment of Jurkat cells with the related cytochalasin D (CytD) ... lead to the question as to how the mAb1418 antibody was selected as being "specific" to Glut-1 According to the manufacturer's indications, this antibody was generated by immunization of mice with...
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Báo cáo y học: " Interaction between human lung fibroblasts and T-lymphocytes prevents activation of CD4+ cells" pdf
... Similarly, the inhibitory effect induced by fibroblasts on T cell CD3 expression has to be ascribed to a direct interaction, as demonstrated by the disappearance of the inhibitory activity in ... Recombinant (Invitrogen), 10 mM dNTPs The following specific primer pairs were used: ICAM-1 sense 5'-GAGCTGTTTGAGAACACCTC-3' and antisense 5'-TCACACTTCACTGTCACCTC-3' giving a 367 bp PCR product; ... lymphocytes co-cultured with fibroblasts, show a significant reduction of the production of tumor necrosis factor-α (TNFα), while the production of interleukin-10 (IL-10) is not affected This condition...
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Xác định sơ bộ giá trị phần trăm, tuyệt đối của tiêu chuẩn quần thể Lympho (T CD3, T CD4, T CD8, B, NK) ở nhóm người bình thường tại thành phố Hồ Chí Minh bằng máy Fascalibur docx
... đ c phân tách sau máy FASC - Kháng thể BD: + CD3 (FITC)/ CD8 (PE) / CD45 (PerCP) / CD4 (APC) + CD3 (FITC) / CD16,56 (PE) / CD45 (PerCP) / CD19 (APC) - Dung dịch ly gi i hồng c u - Máy FASCalibur ... ng i Iran, Châu Âu, TCD3, TCD4, CD19 thấp V i kh c biệt khiến ng i ta suy nghĩ nh vai trò đáp ứng miễn dịch tự nhiên (thông qua NK) quần thể này? Sự cao tỷ lệ NK c giúp ích cho c thể vi c giúp ... premixed monoclonal antibodies:CD3 (FITC)/ CD8 (PE)/ CD45 (PerCP)/ CD4 (APC); CD3 (FITC)/ CD16-56 (PE)/ CD45 (PerCP)/ CD19 (APC) After stain, whole blood is passed lyses procedures, wash and final...
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Báo cáo khoa học: Identification of CD4 and transferrin receptor antibodies by CXCR4 antibody-guided Pathfinder selection pot
... this may also contribute to the steric hindrance In addition, it is unlikely that the size and genetic complexity of the nonimmune library used in this study limited our ability to isolate CXCR4 ... previous report in which CCR5-speci c antibodies were identified by the same selection strategy [10] CXCR4 and CCR5 are the major HIV-1 coreceptors and chemokine receptors They belong to the rhodopsin ... selection (Eur J Biochem 270) 4505 and a cytoplasmic tail (38 amino acids) CD4 and the chemokine receptor CXCR4 were preferentially localized on the microvilli These molecules tend to be found in...
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Báo cáo hóa học: "Quantification of newly produced B and T lymphocytes in untreated chronic lymphocytic leukemia patients" ppt
... number obtained after amplification of coding joints *Abbreviations: IQR, Interquartile range; KRECs, kappa-deleting recombination excision circles; TRECs, T-cell receptor excision circles supported ... Sottini A, Ghidini C, Zanotti C, Chiarini M, Caimi L, Lanfranchi A, Moratto D, Porta F, Imberti L: Simultaneous quantification of recent thymic T-cell and bone marrow B-cell emigrants in patients ... peridin-clorophyll protein-Cy5.5 anti-CD8 and allophycocyanin-H7 anti-CD4), BioLegend (phycoerythrin antiCD25 and peridin-clorophyll protein-Cy5.5 anti-CCR7), eBioscience (phycoerythrin-Cy7 anti-CD127),...
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Báo cáo y học: "Impact of cytokines and T lymphocytes upon osteoclast differentiation and function" pot
... predominantly increases the production of IFN-γ, which is the principal osteoclast inhibitor in response to IL-12 treatment IL-12 and IL-18 act synergistically upon T lymphocytes to inhibit osteoclast ... enhances GM-CSF production and Available online http://arthritis-research.com/content/9/2/103 blockade of GM-CSF production is sufficient to override the inhibitory action of IL-18 In contrast, IL-12 ... survival The importance of IL-17 and IL-23 in rheumatoid arthritis and other inflammatory diseases has been highlighted in studies using knockout mice IL-17 or IL-23 deficient mice were resistant to...
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Báo cáo khoa học:" Expression of Human CD4 and chemokine receptors in cotton rat cells confers permissiveness for productive HIV infection" pptx
... cDNA and isolation infected ratDetection of episomalsequence from HIV- 1of cottoncotton Detection of episomal cDNA and isolation of cotton rat-HIV-1 chimeric DNA sequence from HIV-1 infected cotton ... vaginal simian/ human immunodeficiency virus infection in macaques[27], HSV-2 infection in mice[ 28], gonorrhea in mice[ 29] In recent clinical trials sponsored by NIAID (The HPTN 035 Study of ... of infected cells, [2] detection of episomal viral cDNA in infected cells, [3] demonstration of viral DNA integration into the genome of the cells, and [4] production of infectious viral stocks...
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Báo cáo khoa học: " Expression of Human CD4 and chemokine receptors in cotton rat cells confers permissiveness for productive HIV infection" pdf
... cDNA and isolation infected ratDetection of episomalsequence from HIV- 1of cottoncotton Detection of episomal cDNA and isolation of cotton rat-HIV-1 chimeric DNA sequence from HIV-1 infected cotton ... vaginal simian/ human immunodeficiency virus infection in macaques[27], HSV-2 infection in mice[ 28], gonorrhea in mice[ 29] In recent clinical trials sponsored by NIAID (The HPTN 035 Study of ... of infected cells, [2] detection of episomal viral cDNA in infected cells, [3] demonstration of viral DNA integration into the genome of the cells, and [4] production of infectious viral stocks...
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Báo cáo sinh học: " Streamlined design of a self-inactivating feline immunodeficiency virus vector for transducing ex vivo dendritic cells and T lymphocytes" ppsx
... self-inactivating lentiviral vector expressing simian immunodeficiency virus gag for induction of specific immune responses in vitro and in vivo Viral Immunol 2006, 19:690-701 Chinnasamy N, Treisman ... Matteucci D, Pistello M, Mazzetti P, Giannecchini S, Isola P, Merico A, Zaccaro L, Rizzuti A, Bendinelli M: AIDS vaccination studies using feline immunodeficiency virus as a model: immunisation with ... vectors, FC and BDS were involved in the in vitro characterization of the vector, GF produced and analyzed the dendritic cells, MB was involved in drafting and critical revising the manuscript...
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Báo cáo khoa học: " Early changes of CD4-positive lymphocytes and NK cells in patients with severe Gram-negative sepsis" ppt
... CD4-lymphopenia in severe sepsis The applied flow cytometric analysis of apoptosis did not use propidium iodine staining positive for necrotic cells Application of the analysis was difficult due to the ... study is the first statistically confirming an early increase of NK cells in severe sepsis Animal studies of experimental infections by streptococci concluded that triggering of NK cells was accompanied ... tests Comparisons of qualitative data were performed according to Fischer's test P < 0.05 was considered statistically significant Results Clinical characteristics of the patients enrolled in the...
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Báo cáo y học: "Determinants in HIV-1 Nef for enhancement of virus replication and depletion of CD4+ T lymphocytes in human lymphoid tissue ex vivo" doc
... replicative capacity of simian immunodeficiency virus SIVmac239Delta(nef) is manifest in cultures of immature dendritic cellsand T cells J Virol 2000, 74:2406-2413 Petit C, Buseyne F, Boccaccio C, ... efficiencies were calculated relative to this value with the CD4+ to CD8+ ratio of uninfected cultures considered as 0% depletion For quantification of virus replication, p24 concentrations of individual ... maintain cell composition and cytokine milieu of a primary target organ of in vivo HIV infection [5] Studies in the tonsil HLH model have shed light on key pathogenic properties of HIV, including...
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Báo cáo y học: " Effect of chloroquine on reducing HIV-1 replication in vitro and the DC-SIGN mediated transfer of virus to CD4+ T-lymphocytes" pdf
... that the reduction in infectivity in the presence of Raji-DC-SIGN cells is mediated via the interaction with the DC-SIGN molecule The observed reduction of DC-SIGN mediated transfer could have implication ... significant repercussion on lowering rates of transmission, given the relative inefficiency of infection [35,36] The effect of CQ on DC-SIGN binding in vivo remains to be determined, but if the ... +CQ 100 10 days of replication Figure of CQ Viral replication of C3 3A produced viruses in the presence Viral replication of C3 3A produced viruses in the presence of CQ A) JR-CSF (R5) replication,...
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Báo cáo y học: " Decrease of CD4-lymphocytes and apoptosis of CD14-monocytes are characteristic alterations in sepsis caused by ventilator-associated pneumonia: results from an observational study" ppt
... protection in the event of septic shock? Crit Care 2006, 10:146-154 The ACCP/SCCM Consensus Conference Committee, American College of Chest Physicians/Society of Critical Care Medicine: Definitions ... G, SCCM/ESICM/ACCP/ATS/SIS: 2001 SCCM/ESICM/ACCP/ATS/SIS International Sepsis Definitions conference Crit Care Med 2003, 31:1250-1256 Baughman R: Diagnosis of ventilator-associated pneumonia Curr ... apoptosis of lymphocytes and of monocytes for each pattern of stimulation is shown in Figure Stimulation according to pattern B mimicking pathogenesis of VAP was accompanied by inhibition of apoptosis...
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