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Tài liệu Making the Most of Attaching Mouse Events to Movie Clips pptx
... easier to re-create and implement using an actual button For quick and easy button functionality, use button instances For highly sophisticated button functionality, use movie clip instances with mouse ... appearance when the mouse interacts with it (though you aren't required to this in order to use a movie clip instance as a button) • By default, the hit area of a movie clip button will be the shape ... the hitArea of a movie clip button: • • • myClipButton_mc.hitArea = _root.myHitClip_mc; This property is available only to movie clip buttons, not standard buttons < Day Day Up > ...
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Tài liệu Báo cáo khoa học: Minor capsid proteins of mouse polyomavirus are inducers of apoptosis when produced individually but are only moderate contributors to cell death during the late phase of viral infection ppt
... levels of cytotoxicity of individual VP2 and VP3 variants correlate with their ability to induce apoptosis The highly toxic variants of VP2 and VP3 proved to be very potent inducers of apoptosis The ... USA) Evaluation of cytotoxicity The release of LDH occurring upon cell lysis at different time-points post-transfection of mouse fibroblasts was quantified using a CytoTox 96 cytotoxicity assay kit ... (Fig 4) Cytotoxicity was detected as early as h post-transfection VP2–EGFP and VP3–EGFP were highly toxic As a control, cytotoxicity of VP2– FLAG and VP3–FLAG was measured and found to be of comparable...
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Báo cáo khoa học: Linking environmental carcinogen exposure to TP53 mutations in human tumours using the human TP53 knock-in (Hupki) mouse model doc
... knock-in (Hupki) mouse (Jackson Laboratory Repository designation: 129Trp53tm ⁄ Holl) containing exons 4–9 of human TP53 in place of the corresponding mouse exons (Fig 1) [38] This mouse expresses ... Cre-expressing vector to delete the loxP-flanked neomycin cassette, yielding the final human TP53 knock-in (Hupki) allele ES clones with the Hupki allele were injected into C57BL ⁄ blastocysts to generate ... conditions to favour the mutated cells To select for TP53-mutated cells, Hollstein and coworkers [38,45] Primary exploited the fact that cultured mouse embryo fibroblasts (MEFs), in contrast to human...
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Báo cáo khoa học: Acidic extracellular pH increases calcium influx-triggered phospholipase D activity along with acidic sphingomyelinase activation to induce matrix metalloproteinase-9 expression in mouse metastatic melanoma pot
... (Y Kato, S Ozawa and R I Hata, unpub4 lished data) Because aSMase not only contributes to apoptosis, but also to metastatic ability, its ability to adapt and be selected for resistance to microenvironmental ... of low pH on cytotoxicity of paclitaxel, mitoxantrone and topotecan Br J Cancer 75, 1167–1172 Yamagata M, Hasuda K, Stamato T & Tannock IF (1998) The contribution of lactic acid to acidification ... cytotoxicity of bleomycin, doxorubicin, daunorubicin, epirubicin, mitoxantrone, and vinca alkaloids, but potentiating 5-fluorouracil [1] A recent study demonstrated that acidic pHe is a predictor...
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Báo cáo khoa học: Actin-binding domain of mouse plectin Crystal structure and binding to vimentin pot
... binding occurred to IF subunit proteins in their nonfilamentous state [17–20] Fimbrin was unable to bind to polymerized vimentin in cosedimentation assays and binding occurred at a stoichiometry of ... (Eur J Biochem 271) 1879 Fig Comparisons of mouse and human ABDs Average main chain B factor values of mouse ABDs (A), and differences between Ca atoms in superpositioned structures (B) are plotted ... corresponding Ca atoms ˚ exceeded 1.75 A (Fig 3B, dashed line), the rms displacement values were 0.25 (IB/IA, 232 Ca atoms), 0.48 (II/IA, ˚ 225 atoms), and 0.62 A (HP/IA, 224 atoms) The largest...
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Báo cáo khoa học: A novel mass spectrometric approach to the analysis of hormonal peptides in extracts of mouse pancreatic islets ppt
... islets from the mouse The C-terminal pentapeptide of the rat and human C-peptides is thought to be critical for bioactivity and for binding of the C-peptide to its putative receptor Hence, the ... The desalted peptides were electrosprayed to a Bruker Daltonics BioAPEX)94e 9.4 T Fourier transform ion cyclotron resonance mass spectrometer (Bruker Daltonics, Billerica, MA, USA) [27] using a ... previous study [11] and was also found to be appropriate for this experiment Ten lL of the desalted sample was injected onto the column using a six-port injector valve (Valco Instruments Co Inc.,...
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Báo cáo khoa học: The relative contribution of mannose salvage pathways to glycosylation in PMI-deficient mouse embryonic fibroblast cells pdf
... 4.6 mm; Shodex, Showa Denko KK, Tokyo, Japan) Solvent A was 97% acetonitrile adjusted to pH 7.0 with 0.3% acetic acid Solvent B was 10% acetonitrile adjusted to pH 7.0 with 0.3% acetic acid Gradient ... with weak acid to generate soluble oligosaccharides The hydrolysates were then loaded onto a C18 SepPak column directly connected to a mL ENVI-CARB solid-phase extraction tube to remove residual ... according to the manufacturer’s instructions Briefly, the mutant bovine DNase I cDNAs were subcloned into the pENTER 1A vector After purification of the plasmids, the cDNA inserts were transferred to...
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Báo cáo khoa học: Specific TSC22 domain transcripts are hypertonically induced and alternatively spliced to protect mouse kidney cells during osmotic stress pptx
... GADD45 genes [59], TonEBP transcription factor [46] and aquaporin [61] in response to hypertonicity mRNA stabilization is a regulatory mechanism involved in rapid responses to various forms of ... into the genome (mIMCD3FRT cells; Fig S1) to generate a good control for future experiments with stably selected cells We then cotransfected V5-epitope-tagged TSC22D2-4 in pcDNA5FRT vector together ... transcription of HSP70 in response to hypertonicity Mol Cell Biol 22, 5753–5760 Nakayama Y, Peng T, Sands JM & Bagnasco SM (2000) The TonE ⁄ TonEBP pathway mediates tonicity-responsive regulation...
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guide to techniques in mouse development
... the irradiated stocks can be frozen down for storage Our STO cells are subjected to a total dose of 50-55 Gy (>5000 rad), from an X-ray machine Other methods for mitotic arrest of STO cells are ... are given to another investigator, they are " m o v e d " into an alphabetic icon for each If you have additional information to add to a record, you can it within the context of this protocol Supplemental ... mating It is possible to set aside a set of cage numbers for stocks of females, combining females from many different stocks together in the same "storage cages" according to age, for example (In...
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Báo cáo hóa học: " Amphotropic murine leukemia virus is preferentially attached to cholesterol-rich microdomains after binding to mouse fibroblasts" pot
... 335:433-440 Giurisato E, McIntosh DP, Tassi M, Gamberucci A, Benedetti A: T cell receptor can be recruited to a subset of plasma membrane rafts, independently of cell signaling and attendantly to raft clustering ... comparison to A-MLV, neither VSV nor viral like particles lacking viral envelope proteins showed preferential attachment to large rafts Thus, while binding of A-MLV to the large raft regions seems to ... proteins and an YFP-tagged nucleocapsid protein [12], pHIT111 (β-galactosidase encoding retoviral vector) [23], and a pHIT derived vector encoding the A-MLV (4070A isolate) envelope or a plasmid encoding...
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Báo cáo hóa học: " Application of functional genomics to the chimeric mouse model of HCV infection: optimization of microarray protocols and genomics analysis" potx
... ratio of human to mouse RNA It has previously been established that the transplanted human hepatocytes develop into red nodules within the mouse liver, which is much paler in color [4] To obtain liver ... of contaminating mouse tissue Therefore, RNA isolated from these samples for use in microarray experiments will contain RNA from mouse hepatocytes To identify genes affected by mouse liver (ML) ... animals was compared to the respective uninfected controls The microarray experiments showed increased expression of these genes in Mouse and, to a lesser extent, Mouse (Figure 2A) Mouse showed decreased...
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báo cáo hóa học:" Progressive obesity leads to altered ovarian gene expression in the Lethal Yellow mouse: a microarray study" pptx
... tended to be recovered than from identically-treated black mice [14] To elucidate whether impaired fertility in aging LY mice was due to intrinsic ovarian defects or to extraovarian factors, Granholm ... (Molecular Probes/Invitrogen) to label the cRNA and washed again An Axon GenePix Scanner was used to scan the microarrays GenePix Pro software (MDS, Inc., Toronto, ON) was used to acquire and align the ... were calculated relative to an endogenous control (GAPDH) An RNA concentration-response validation curve was carried out to determine the concentration of RNA to add to the RT-PCR reaction All...
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báo cáo hóa học:" Microarray analysis of Foxl2 mediated gene regulation in the mouse ovary derived KK1 granulosa cell line: Over-expression of Foxl2 leads to activation of the gonadotropin releasing hormone receptor gene promoter" pptx
... factors including histone acetylases, basal transcription factors, and the coactivators CBP and Mediator to name a few [20,21] Therefore, over-expression of VP16 fusion proteins may lead to repression ... base pair fragment of the mouse GnRHR promoter into the expression vector pGL3 basic (Promega Corporation; Madison, WI) The Foxl2 expression vector (pFoxl2) consists of the mouse Foxl2 open reading ... list of genes resulted in finding a total of 42 genes common to both (Additional file 10) In order to begin to understand the significance of the genes common to the three microarray studies in...
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Báo cáo khoa học: "Protective effect of the isoflavone equol against DNA damage induced by ultraviolet radiation to hairless mouse skin" potx
... loibotohP mehcotohP J sisenegonicracotohp dna riaper AND ,ygolonummiotohP J nnamturK ,M grubenreB 904-504 ,011 ,8991 lotamreD tsevnI J amede dna amehtyre decudni BVU ot ytivitisnes htiw ton tub ... stceffe evitcetorpotohp eht suht ,]82[ ecim ni seitreporp cinegonicracotohp -itna evah ot detartsnomed neeb sah louqe deilppa yllacipot dna ,sromut detaitini-noitaidar VU fo noitomorp eht rof ... lamred dna lamredipe ni htob ,ylralullecartni 122 ortiv ni dnuof erew sDPC ,snoitidnoc latnemirepxe ruo rednU ]41,5[ noisserppusonummiotohp dna sisenegonicracotohp htob ni elor laicurc a yalp...
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Báo cáo khoa học: " Effect of dihydrotestosterone on mouse embryonic stem cells exposed to H2O2-induced oxidative stress" pdf
... 3.7 g/l sodium bicarbonate, 1% penicillin and streptomycin, 1.7 mM L-glutamine, 0.1 mM β-mercaptoethanol, ng/ml mouse leukemia inhibitory factor and 15% FBS The cells were grown on either gelatinized ... experiments Effect of dihydrotestosterone on mouse embryonic stem cells exposed to H2O2-induced oxidative stress 251 Fig Effect of hydrogen peroxide (H2O2)-induced p38 mitogen activated protein kinase ... increase of cell cycle regulatory protein expression, the mouse ES cells were pretreated with DHT for 30 prior to adding the H2O2 The H2O2-induced decrease of cell cycle regulatory protein (cyclin D1,...
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Báo cáo y học: "Epitope spreading to citrullinated antigens in mouse models of autoimmune arthritis and demyelination" pot
... demonstrate epitope spreading of autoantibody responses to target and citrullinated epitopes in experimental autoimmune encephalomyelitis (EAE) Myelin arrays demonstrate epitope spreading of autoantibody ... mice Epitope spreading of autoantibody responses in EAE We used 406 antigen myelin proteome arrays to examine the pattern of epitope spreading of the autoantibody response in EAE Epitope spreading ... Autoantigen microarrays for multiplex characterization of autoantibody responses Nat Med 2002, 8:295-301 The Robinson Lab Protocols and Tools [http://robinson lab.stanford.edu/protocols/] Storey...
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Báo cáo y học: "Hedgehog overexpression leads to the formation of prostate cancer stem cells with metastatic property irrespective of androgen receptor expression in the mouse model" pps
... membrane localizations (Figure 2F to 2G and 2K to 2L), while Gli transcription factors were predominantly detected in the nucleus and cytoplasm (Figure 2H to 2J and 2M to 2O) Moreover, Hh signaling ... induced into PIN and progressed into CaP status, these P63 + /CK14(low or -) cells appeared to be differentiated into P63+/CK14+ (Figure 5C; arrowhead-indicated in (b)) and further into P63 (low) ... presence of prostate progenitor/stem cells in these purified cell populations and their ability to regenerate in ectopic sites Such ectopic regeneration capacity has been proven to retain even after...
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Báo cáo y học: "Amino acid substitutions in the E2 glycoprotein of Sindbis-like virus XJ-160 confer the ability to undergo heparan sulfate-dependent infection of mouse embryonic fibroblasts" docx
... been reported to be able to block Sindbis virus to infect and form plaques in cells [7] Bacterial produced heparinase I that cleaves heparin and cellular HS has been commonly used to remove cell ... interact with proteins (16), the reduced infectivity of the viruses to MEF-Epi-/- cells in comparison to the MET-wt cells is likely due to the structural alterations of the surface HS that has a weaker ... aberrant interaction with growth factors Further studies to test the infection of the viruses on a 2-O-sulfotransferase mutant cell may provide additional information to this end Heparin, an analog...
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Báo cáo y học: "Use of a highly sensitive strand-specific quantitative PCR to identify abortive replication in the mouse model of respiratory syncytial virus disease" pptx
... either the BALB/c mouse [15] or the cotton rat (Sigmodon hispidus) [16] models The cotton rat is moderately permissive to human respiratory viral infection and RSV is able to replicate and produce ... chain reaction (QPCR) method to monitor the kinetics of RSV RNA replication in the mouse lung BALB/c mice were infected with RSV A2 and viral RNA in mouse lungs were monitored over an extended time ... insight into RSV RNA production in infected BALB/c mouse lung To our knowledge, this is the first time that a strand specific method has been applied to profile RSV RNA dynamics in the BALB/c mouse...
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Báo cáo y học: " A mouse model to study infection against porcine circovirus type 2: viral distribution and lesions in mouse" docx
... in a mouse is still a debated topic Therefore, Kunming mouse were chosen as the experimental animal for our research Results of this study demonstrate that PCV2 can replicate in Kunming mouse ... Molecular Cloning: A Laboratory Manual Cold Spring Harbor Laboratory Press, New York, 2001 doi:10.1186/1743-422X-7-158 Cite this article as: Li et al.: A mouse model to study infection against ... regularity First, it has taken at least days for the PCV2 to be transferred into other organs from the primary interface It seems that the diffusion to thymus had been retarded for days The replication...
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