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báo cáo hóa học:" A general method for nested RT-PCR amplification and sequencing the complete HCV genotype 1 open reading frame" pdf
... CentralPage 1 of 9(page number not for citation purposes)Virology JournalOpen AccessMethodologyA general method for nested RT-PCR amplification and sequencing the complete HCV genotype 1 open ... Thisstrategy was effective for amplifying all amplicons except for amplicon 4 for both genotype 1a and 1b and amplicon1 for genotype 1b. To increase the sensitivity of amplifica-tion for these regions, ... of sufficient DNA for sequencing with any one of the primer pairs for a given amplicon) for amplification of 72 genotype1a and 1b patient plasma samples averaged over 95% for all amplicons....
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Tài liệu Báo cáo khoa học: A facile method for expression and purification of the Alzheimer’s disease-associated amyloid b-peptide pdf
... thanrequired for thioflavin T (ThT)-based fibrillationassays (typical concentrations used are 3–10 lm), butis not sufficient for other biophysical studies. Wetherefore examined a number of methods ... applied to acarbon-coated formvar grid, left for 1 min, fixed with glu-taraldehye, wicked dry with filter paper, and 2% uranylacetate was added and the mixture was incubated for 2 min. The grid was ... filtrate from fractions eluted at 150–200 mmNaCl. For both Ab(M1–40) and Ab(M1–42), allmanipulations were performed at slightly alkaline pHto avoid the formation of structural contaminants pro-duced...
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Tài liệu Báo cáo khoa học: Development of a new method for isolation and long-term culture of organ-specific blood vascular and lymphatic endothelial cells of the mouse pdf
... lm.A new method for mouse endothelial cell culture T. Yamaguchi et al.1990 FEBS Journal 275 (2008) 1988–1998 ª 2008 The Authors Journal compilation ª 2008 FEBSDevelopment of a new method for isolation ... University of Tokyo, Japan) for providingpCAG-Cre; Dr Ikuo Yana (Institute of MedicalScience, University of Tokyo, Japan) for help in per-forming endothelial tube formation experiments. Thiswork ... Tokyo,Japan). For immunocytochemistry, cells were fixed on ice with4% PFA in NaCl ⁄ Pi for 10 min, incubated in methanol at)20 °C for 20 min, and rehydrated in NaCl ⁄ Pi. For detec-tion...
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Tài liệu Báo cáo khoa học: "A New Dataset and Method for Automatically Grading ESOL Texts" pdf
... of the Association for Computational Linguistics, pages 180–189,Portland, Oregon, June 19-24, 2011.c2011 Association for Computational LinguisticsA New Dataset and Method for Automatically ... dataset for training and test-ing such systems and comparing their performance.As it is likely that the deployment of such systemswill increase, standardised and independent evalua-tion methods ... style, grammar, and so forth, thoughthe methods used to assess these are not describedin any detail in published work. Again, the systemrequires retraining or tuning for new prompts andassessment...
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Tài liệu Báo cáo khoa học: "An Ensemble Method for Selection of High Quality Parses" pdf
... 100. For future work, integrating SEPA into the rerank-ing process seems a promising direction for enhanc-ing overall parser performance.Acknowledgement. We would like to thank DanRoth for ... normalizing oversentence lengths is less of a factor. For this reason,in this paper we present detailed graphs for the aver-age f-score. For completeness, Table 4 also providessome of the results ... al., 2006). For completeness of reference, Table 4 shows thesuperiority of SEPA over CB in terms of the usual f-score measure used by the parsing community (num-bers are counted for constituents...
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Tài liệu Báo cáo khoa học: "A Tool for Multi-Word Expression Extraction in Modern Greek Using Syntactic Parsing" pdf
... (Seretan, 2008) which has previously been1 For the sake of simplicity, we will henceforth use theterm Greek to refer to Modern Greek.used to build MWE resources for other languages,including English, ... rules defined for Greek, allowing for the complete parse of about50% of the sentences in a corpus like Europarl(Koehn, 2005), which contains proceedings ofthe European Parliament. For the remaining ... 45–48,Athens, Greece, 3 April 2009.c2009 Association for Computational LinguisticsA Tool for Multi-Word Expression Extraction in Modern GreekUsing Syntactic ParsingAthina MichouUniversity...
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Spring Data Modern Data Access for Enterprise Java pdf
... like Example 2-13. It containsmethods to save a single entity as well as an Iterable of entities, finder methods for asingle entity or all entities, and delete(…) methods of different flavors.Example ... and scientific laboratories had been performing this process beforethe term big data came into fashion. What makes the current process different frombefore is that the value derived from the ... anyway) for properties and parse the method name into aproperty traversal tree. To avoid these kinds of typos as early as possible, STS’s SpringData support offers code completion for property...
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Novel method for sputum induction using the Lung Flute in patients with suspected pulmonary tuberculosis pdf
... Flute for sputum sampling in patients suspectedof pulmonary tuberculosis (TB).Methods: Thirty-four patients who were not expecto-rating sputum, but for whom sputum examination wasrequired for ... Capilia TB assay-positive.‡See text for explanation of further examinations for the diagnosis of TB.AFB, acid-fast bacillus; TB, tuberculosis.Novel method for sputum induction in TB 901© 2009 ... has yet to be cleared for use inpatients by the Japanese authority. Individual LungFlutes were supplied for each patient by MedicalAcoustics, Tokyo, Japan. Written informed consentwas obtained...
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Báo cáo sinh học: " A general method for nested RT-PCR amplification and sequencing the complete HCV genotype 1 open reading frame" potx
... CentralPage 1 of 9(page number not for citation purposes)Virology JournalOpen AccessMethodologyA general method for nested RT-PCR amplification and sequencing the complete HCV genotype 1 open ... Thisstrategy was effective for amplifying all amplicons except for amplicon 4 for both genotype 1a and 1b and amplicon1 for genotype 1b. To increase the sensitivity of amplifica-tion for these regions, ... of sufficient DNA for sequencing with any one of the primer pairs for a given amplicon) for amplification of 72 genotype1a and 1b patient plasma samples averaged over 95% for all amplicons....
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Book Manual Getting Started Guide for Peachtree Complete Accounting_13 pdf
... payroll reports, 941 forms, W-2 forms, and W-3 forms. For more information, see “Assign W-2 Payroll Fields in Peachtree Accounting” on page 266.Assign Payroll Fields for EmployEE- and EmployER-Paid ... use department masking. For more information, refer to the “General Ledger” chapter of the Peachtree Accounting User’s Guide.Budget InformationBudget information for each QuickBooks account ... Peachtree Accounting as stock items using the costing method selected during the conversion process. For more information about costing methods, refer to the “Inventory” chapter of the Peachtree...
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Book Manual Getting Started Guide for Peachtree Complete Accounting_14 pdf
... InformationAdding or Editing Company InformationCompany/Company Info Maintain Company Information windowConverting QuickBooks Data to Peachtree AccountingGetting Started Guide278B For ... number. Then select Post or Save to record the transaction. For more information on entering payments, see Pay Vendors. For more information on entering receipts, see Receive a Payment from a ... select Default Information from the Maintain menu. Then, select Employees. The Employee Defaults window displays. For detailed information on what and how QuickBooks job information is converted...
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Báo cáo hóa học: " A stabilized mixed discontinuous Galerkin method for the incompressible miscible displacement problem" pdf
... stabilized mixed DG method for the velocity and pressure4.1 Elimination for the flux variable uLetting a(c)=a(c)-1. For the velocity and pressure, we define the following formsa(u, v; c)=(α(c)u, ... (Dl−2(Th))d× Dl−1(Th),(6:18)where (6.17) clearly holds for every θ Î (0,1) for the case ((6.4 ), (6.8)), and for everyθ < 0 for the case ((6.10), (6.13)). On the other hand, since ∇hDl-1(Th) ... mixed finite element (MFE) method for the compressible miscible displacement problem. For the Darcy flow,Masud and Hughes [2] introduced a stabilized finite element formulation in which anappropriately...
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Báo cáo hóa học: " Research Article A New Method for Least-Squares and Minimax Group-Delay Error Design of Allpass Variable Fractional-Delay Digital Filters" pdf
... will lead to the samesolution. The formal formulation for LS group-delay errordesign of allpass VFD filters will be presented in Section 3,inwhich an iterative method is proposed to replace the ... complexity, althoughthe design time of the proposed method is much morethan the existing methods, an alternative method has beenrevealed in this paper for further research in the future.References[1] ... denominator in (22) is ignored for theiterative method in (23), which will yield satisfactory results.Equation (23) can be further represented in a quadratic formasek(ak)= sτ+ aTkQsak+...
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