... thanrequired for thioflavin T (ThT)-based fibrillationassays (typical concentrations used are 3–10 lm), butis not sufficient for other biophysical studies. Wetherefore examined a number of methods ... applied to acarbon-coated formvar grid, left for 1 min, fixed with glu-taraldehye, wicked dry with filter paper, and 2% uranylacetate was added and the mixture was incubated for 2 min. The grid was ... filtrate from fractions eluted at 150–200 mmNaCl. For both Ab(M1–40) and Ab(M1–42), allmanipulations were performed at slightly alkaline pHto avoid the formation of structural contaminants pro-duced...
... lm.A new methodfor mouse endothelial cell culture T. Yamaguchi et al.1990 FEBS Journal 275 (2008) 1988–1998 ª 2008 The Authors Journal compilation ª 2008 FEBSDevelopment of a new methodfor isolation ... University of Tokyo, Japan) for providingpCAG-Cre; Dr Ikuo Yana (Institute of MedicalScience, University of Tokyo, Japan) for help in per-forming endothelial tube formation experiments. Thiswork ... Tokyo,Japan). For immunocytochemistry, cells were fixed on ice with4% PFA in NaCl ⁄ Pi for 10 min, incubated in methanol at)20 °C for 20 min, and rehydrated in NaCl ⁄ Pi. For detec-tion...
... of the Association for Computational Linguistics, pages 180–189,Portland, Oregon, June 19-24, 2011.c2011 Association for Computational LinguisticsA New Dataset and Methodfor Automatically ... dataset for training and test-ing such systems and comparing their performance.As it is likely that the deployment of such systemswill increase, standardised and independent evalua-tion methods ... style, grammar, and so forth, thoughthe methods used to assess these are not describedin any detail in published work. Again, the systemrequires retraining or tuning for new prompts andassessment...
... 100. For future work, integrating SEPA into the rerank-ing process seems a promising direction for enhanc-ing overall parser performance.Acknowledgement. We would like to thank DanRoth for ... input for systems that require high quality parses,what matters most is that SEPA outperforms the MRbaseline at the high k ranges.Figure 4 (bottom) shows the same pattern of re-sults for the ... Linguistics, pages 408–415,Prague, Czech Republic, June 2007.c2007 Association for Computational LinguisticsAn Ensemble Methodfor Selection of High Quality ParsesRoi ReichartICNCHebrew University...
... Practices for SamplingWater,” American Society for Testing and Materials, Philadelphia.9. “Methods 330.4 (Titrimetric, DPD-FAS) and 330.5 (Spectrophotometric, DPD) for ChlorineTotal Residual,” Methods ... establishedperformance criteria to determine if the results of analyses meet the performancecharacteristics of the method. When results of sample spikes indicate atypical method performance, a ... using the extract produced by this method. 1.3 The method detection limit (MDL, defined in Section 14.1) for each parameter is listed1in Table 1. The MDL for a specific wastewater may differ...
... Thisstrategy was effective for amplifying all amplicons except for amplicon 4 for both genotype 1a and 1b and amplicon1 for genotype 1b. To increase the sensitivity of amplifica-tion for these regions, ... percentage.BioMed CentralPage 1 of 9(page number not for citation purposes)Virology JournalOpen AccessMethodologyA general methodfor nested RT-PCR amplification and sequencing the complete ... of sufficient DNA for sequencing with any one of the primer pairs for a given amplicon) for amplification of 72 genotype1a and 1b patient plasma samples averaged over 95% for all amplicons....
... stabilized mixed DG methodfor the velocity and pressure4.1 Elimination for the flux variable uLetting a(c)=a(c)-1. For the velocity and pressure, we define the following formsa(u, v; c)=(α(c)u, ... (Dl−2(Th))d× Dl−1(Th),(6:18)where (6.17) clearly holds for every θ Î (0,1) for the case ((6.4 ), (6.8)), and for everyθ < 0 for the case ((6.10), (6.13)). On the other hand, since ∇hDl-1(Th) ... mixed finite element (MFE) method for the compressible miscible displacement problem. For the Darcy flow,Masud and Hughes [2] introduced a stabilized finite element formulation in which anappropriately...
... will lead to the samesolution. The formal formulation for LS group-delay errordesign of allpass VFD filters will be presented in Section 3,inwhich an iterative method is proposed to replace the ... complexity, althoughthe design time of the proposed method is much morethan the existing methods, an alternative method has beenrevealed in this paper for further research in the future.References[1] ... denominator in (22) is ignored for theiterative method in (23), which will yield satisfactory results.Equation (23) can be further represented in a quadratic formasek(ak)= sτ+ aTkQsak+...
... hi1/2 be an average mesh size.Before describing our numerical method, we introduce some notation for the mesh functions.Define the following finite differences for any mesh function vi vxi ... for an ordinary d ifferential equation,” Computational Mathematics and Mathematical Physics,vol. 35, no. 11, pp. 1417–1422, 1995.8 G. F. Sun and M. Stynes, “A uniformly convergent methodfor ... 2010, Article ID 102484, 13 pagesdoi:10.1155/2010/102484Research ArticleUniform Second-Order Difference Method for a Singularly Perturbed Three-PointBoundary Value ProblemMusa C¸ akırDepartment...
... heterogeneity such that for Δ(I)> 15 dB, SIC is clearly superior to OMA.The beneficial effects of the newly proposed strategy canbe observed for BORG∗K, which performs as well as OMA for small Δ(I), ... not use formula (7.31)from [3] because it was derived for SIC with residual errorspropagated from previous steps.It is clear that, for T∈{2, , K − 1}, we do not needto compute AME for all ... in which the groups are decoded.Remark for T= 1 that the grouping method is the samewith OMA. Moreover, the g rouping method is identical withSIC for T= K. Similarly to conventional SIC,...
... 21).Figure 21 An example pseudo-section [11].2.3 The forward problem by differential method Fully analytical methods have been used for simple cases of the forward modeling problem, such as a sphere ... direct current method. This method is relatively new compared to other geophysical methods. Since the 1970s, extensive research has been done on the inversion theory for this method and it is ... specification came out in 1997 with support for Fortran, followed by C/C++ support in 1998. Version 2.0 was released in 2000 for Fortran and 2002 for C/C++. Version 3.0 was released in 2008...