khung truyen tin duoc su dung trong viec thu thap so lieu
Báo cáo y học: "Post-translational aging of proteins in osteoarthritic cartilage and synovial fluid as measured by isomerized aspartate" pot
... USA) Determination of isomerized aspartate content IsoAsp content was measured using the commercially available ISOQUANT® IsoAspartate Detection Kit (Promega Corporation, Madison, WI, USA) in accordance ... form of IsoAsp, in OA-affected human cartilage and synovial fluid and to examine associations between IsoAsp content and the degree of OA-related tissue damage We found significantly more IsoAsp ... Thus, the decline in IsoAsp from a tissue or body fluid could be due to conversion to a racemized form or due to loss from tissue With these data, we show that it is due to loss from the tissue...
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Post translational regulation of the human TRIP br1 cell cycle protein
... proteosomal pathway MDM2 is an E3-ubiquitin ligase and catalyses the mono-ubiquitination of p536 MDM2 is also capable of auto-ubiquitination The choice between targeting p53 or itself for ubiquitination ... protein-protein interactions and, in some instances, DNA binding (Figure 12) Mammalian cells have types of SUMO modifications, designated SUMO-1, SUMO-2 and SUMO-3 The sumolyation pathway appears to ... deacetylating p53 at Lys382, resulting in a concomitant decrease in p53-mediated transcriptional activity [77] Inhibition of the SIRT1 deacetylase resulted in p53 stabilization Notably, CBP/p300 also...
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Tài liệu Báo cáo khoa học: Complex transcriptional and translational regulation of iPLA2c resulting in multiple gene products containing dual competing sites for mitochondrial or peroxisomal localization docx
... of multiple distinct isoforms of iPLA2 in the cytosolic, microsomal and mitochondrial compartments in human heart [7,8] Among the membrane-associated activities identified was a subset that localized ... repressor region supported a conclusion that binding by multiple, as yet unidentified, nuclear proteins occurs which could result in transcriptional modulation of iPLA2c These results strongly support ... dithiothreitol, 0.34 M sucrose, 20 lM trans-epoxysuccinyl-L-leucylamido(4-guanidino) butane and lgÆmL)1 leupeptin) Cells were lysed by sonication (20 · s bursts utilizing a Vibra-cell sonicator at 30%...
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Tài liệu Báo cáo khoa học: Post-translational cleavage of recombinantly expressed nitrilase from Rhodococcus rhodochrous J1 yields a stable, active helical form ppt
... dihydratases, and also leads to definite suggestions about the interacting residues Examination of the complexes formed prior to post-translational cleavage suggests that steric hindrance resulting from ... central channel There is some vacant density in this region which may accommodate residues 314–327 There is also sufficient vacant density between the subunits in the C surface region to accommodate ... sulfate or organic solvent [16–18] It is interesting that the active 480-kDa complex formed readily from the recombinant enzyme but was not isolated from the native organism [16] However, a substantially...
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Báo cáo Y học: Regulation of RAS in human platelets Evidence that activation of RAS is not sufficient to lead to ERK1-2 phosphorylation pot
... 60, 120 or 240 s Triton X-100 soluble and insoluble fractions were isolated Proteins of both fractions were resolved by 12.5% SDS/PAGE and analysed by Western blotting using an anti-RAS Ig Ó ... 300 mM NaCl, mM EDTA, 2% (v/v) NP40, mM phenylmethanesulfonyl fluoride, mM Na3VO4, 10 lgÆmL)1 leupeptin, 10 lgÆmL)1 aprotinin, lgÆmL)1 pepstatin A, pH 7.3) Lysed cells and debris were removed by ... activation was measured through the ability of its activated form (RAS–GTP) to bind to a GST fusion protein consisting of the RAS-binding domain of RAF and subsequent detection by Western blotting To prevent...
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Báo cáo khoa học: Factor-inhibiting hypoxia-inducible factor (FIH) catalyses the post-translational hydroxylation of histidinyl residues within ankyrin repeat domains pot
... hydroxylation sites, are also substrates for FIH-catalysed b-hydroxylation In vitro biochemical studies suggest that FIH may also catalyse His hydroxylation in other ARDs The results expand the scope ... residue in ankyrinR [14], the results presented here suggest that the substrate selectivity of FIH may be even wider than previously perceived The combined results also raise the possibility that ... the ferric uptake repressor, PerR in Bacillus subtilis, which is inactivated by oxidation of the imidazole rings of two Fe(II)-binding histidinyl residues resulting in suppression of peroxidedefence...
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Báo cáo sinh học: "Regulation of metabolism in Caenorhabditis elegans longevity" ppsx
... dilution of nutrients, or by exposure to axenic medium (medium that supports growth without bacteria), actually had increased metabolic rates, as measured by oxygen consumption and heat production ... affecting complexes I, II and III of the electron transport chain (ETC), including some that increase life span, also result in accumulation of branched-chain amino acids Mutations that confer longevity ... regulates life span in worms is therefore not clear While it seems to act by regulating the insulin pathway in Drosophila and rats, the IIS path way is not responsible for dietary-restriction-induced...
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Báo cáo y học: "Expression and regulation of CCL18 in synovial fluid neutrophils of patients with rheumatoid arthritis" potx
... TNF-α, suggesting that TNF-α alone may not be sufficient to prime PMN for CCL18 synthesis This conclusion is supported by data showing that CCL18 production could be induced by stimulating PMN ... preprocessing Digital images resulting from post-hybridization array scanning were quantified with GenePix Pro 4.0 software (Axon Instruments) GenePix™ Analysis Software was used for spot detection ... form RA patients (n = 9; Figure 2b), suggesting that CCL18 gene expression in SF PMN occurs as a result of the recruitment of PMN into the inflammatory milieu of the joint Differential expression...
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Báo cáo y học: "Expression and regulation of neurotrophins in the nondegenerate and degenerate human intervertebral disc" pdf
... both the neural growth-associated marker GAP43 and the pain-associated neuropeptide substance P [5] Additional studies investigating innervation in the degenerate IVD have also demonstrated expression ... – indicating that such nerves are NGF sensitive [4] This is supported by Aoki and colleagues, who have identified NGF-responsive nerve fibres as the predominant neuronal subtype innervating the ... intervertebral disc tissue tissue Nerve growth factor (NGF) protein expression was identified in (a), (c) the chondrocyte-like cells of human nucleus pulposus and inner annulus fibrosus tissue, and (e)...
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Cyclic nucleotides and mitogen-activated protein kinases: regulation of simvastatin in platelet activation pptx
... simvastatin 30 50 50 simvastatin 50 50 SQ 22536 + + SQ 22536 + E collagen simvastatin 50 simvastatin 50 ODQ DMSO collagen simvastatin 50 simvastatin 50 SQ22536 ǻT(10%) DMSO ... (B) For the electron spin resonance (ESR) study, platelets were preincubated with (a) Tyrode's solution (resting group), (b) a solvent control (0.5% DMSO), or simvastatin (30 and 50 μM), followed ... to the suspensions The thromboxane B2 (TxB2) levels of the supernatants were measured using an EIA kit Immunoblotting study Washed platelets (1.2 × 109/ml) were preincubated with simvastatin (30...
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Báo cáo y học: "Stanniocalcin-1 promotes tumor angiogenesis through up-regulation of VEGF in gastric cancer cells" ppt
... supernatants were collected, centrifuged at 4°C, 4000 rcf for 10 min, and stored at -70°C for subsequent use Tumor supernatants were labeled as BGC supernatant, BGC/CON supernatant, BGC/STC supernatant, ... tubes (Figure 3A, C) These results suggest that STC-1 may change some factors of tumor microenvironment to modulate angiogenesis VEGF is neceseary to STC-1 promoting angiogenesis It is well known ... pro-plus software All histogram was carried out on multiple sections and the results are representative of three independent experiments (E) effect of isotype antibody on cell migration IS: isotype...
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Báo cáo y học: " Post-translational modifications of histones H3 and H4 associated with the histone methyltransferases Suv39h1 and G9a" pdf
... chromatin enriched in mononucleosomes (Figure 2a and Additional data file 1) The Suv39h1-associated complex is visualized in Figure 2b, lane We observe good stoichiometry of the Suv39h1-associated ... created by Suv39h1, recruit Suv4-20h, the enzyme that normally establishes H4K20me3 Our results suggest that Suv39h1 is preferentially associated with H4K20me2, but not H4K20me3 This association ... histone H4 associated with both Suv39h1 and G9a It is quite surprising to have H4K16ac associated with the transcription repressors Suv39h1 and G9a, since this modification is mainly associated with...
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Báo cáo y học: "Global nucleosome distribution and the regulation of transcription in yeas" doc
... nucleosomes at the promoters of ribosomal protein genes [15], so one prediction is that the loss of nucleosomes may be a result of Rap1p binding at the promoters This idea is supported by the results ... hour have a lower density of nucleosomes than ORFs overall This is an important observation because it suggests not only that nucleosomes are transiently dissociated from DNA during the elongation ... fractionation of yeast chromatin suggests that the chromatin is organized differently between coding and noncoding regions of the genome, and the heterogeneous distribution of nucleosomes may be part of...
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Báo cáo y học: "Translational regulation of gene expression" docx
... described the identification and visualization of the protein RACK1 as a component of the small ribosomal subunit in the fungus Thermomyces lanuginosus RACK1 (also called Asc1p in Saccharomyces ... 40S subunit and when the elongation factor eEF2 binds to the 80S ribosome This suggests that the CrPV IRES functions as an RNA-based translation factor that actively manipulates the ribosome ... to mediate the virus’s unusual mode of translation initiation Collectively, the structural data on the ribosome and its associated complexes presented during the meeting led the audience to an...
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REGULATION OF CHEMICALS IN EUROPE – REACH
... exposure for each use Develop exposure scenario for each use 19 EXPOSURE SCENARIOS-WORKERS • Work process – descriptor • Time of activity • % of substance used • Form of substance (gas, liquid, solid) ... controlled, socioeconomic benefits outweigh risk, no suitable alternative substitute Registration 11 SCOPE - DEFINITIONS Substance: Single chemical compound – CAS-number Preparation: Solutions ... (PROC 2) Type of setting – industrial Form of substance – liquid Duration of exposure > hrs (default) Location indoor without ventilation Inhalation exposure 66.8 mg/m3 Dermal exposure 1.4 mg/kg/day...
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Understanding the regulation of cytokinesis in fission yeast
... alpha-actinin prevents actin turnover and inhibits cytokinesis; in contrast, depletion of alpha-actinin promotes cytokinesis (Mukhina et al., 2007) Thus, alpha-actinin and possibly other Factin associated ... like to thank my advisor, Dr Mohan Balasubramanian, for giving me the opportunity to pursue a PhD in his laboratory I am also grateful for his constant encouragement, continuous support to my projects ... After chromosome segregation, the activity of cyclinB/CDK1 is down-regulated to promote cytokinesis A multi-subunit E3 ubiquitin ligase, known as anaphase promoting complex/cyclosome (APC/C),...
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Role and regulation of juxtanodin in actin cytoskeleton of oligodendrocyte
... to G-actin, therefore enhancing 11 Introduction the nucleating activity of Arp2/3 by feeding actin monomers (Suetsugu et al., 2002) In association with cortactin, verprolin/WASP-interacting protein ... of actin monomers on it, terminating the F-actin extension Filaments age through hydrolysis of ATP bound to actin subunit (white subunits turn yellow) and dissociation of the phosphate (subunits ... yeasts, have only one actin protein, whereas higher eukaryotes usually have several actin isoforms In plants, more than 100 different actin genes coding for at least actin isoforms were found (McLean...
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Regulation of cytokinesis in the fission yeast schizosaccharomyces pombe
... dimethyl sulphoxide (DMSO) Latrunculin A (Molecular Probes) was dissolved in DMSO to make a 10 mM stock solution and was used at 0.2 mM final concentration DMSO was used as a corresponding solvent ... ring disassembles and continuous rounds of nuclear division occur in the absence of cytokinesis resulting in formation of multinucleated cells (Balasubramanian et al 1998) Thus, the SIN has an essential ... my supervisor Prof Mohan Balasubramanian for giving me the opportunity to work in his lab I will always cherish his gentle mentorship, his infectious enthusiasm for science and unflagging support...
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THE ROLE OF THE CTD PHOSPHATASE RTR1 AND POST-TRANSLATIONAL MODIFICATIONS IN REGULATION OF RNA POLYMERASE II
... ubiquitination and subsequent proteasomal degradation on other RNAPII subunits Multiple studies have suggested that the cytotoxic effect of proteasome inhibitors on tumor cells is related to suppression ... Cdk8 (Srb10) Rtr1, Ssu72 Early Elongation RPAP2, Scp1, Ssu72?, Cdc14 Cdk7, Cdk9 Ssu72? Kin28, Bur1 Ssu72 Early Elongation (yeast Rtr1)/Late Elongation/Termination (yeast Ssu72) Early Elongation ... for degradation by the ubiquitin/proteasome degradation pathway through addition of polyubiquitin chains (Somesh et al., 2007; Y Zhang et al., 2009) In fact, two ubiquitin sites have been identified...
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