isolation and characterization of human prostate stem progenitor cells
Báo cáo y học: "Isolation and characterization of human cells resistant to retrovirus infection" pdf
... and mutant cells and extent of HIV Growth Growth rates of parental and mutant cells and extent of HIV integration (A) Growth rates Parental and mutant 30-2 and 42-7 cells were seeded and growth ... x 105 Figure cells with an HIV-EGFP vector at a moi = 0.5 Infection of parental and mutant HeLa cells (30-2 and 42-7) Infection of parental and mutant HeLa cells (30-2 and 42-7) cells with an ... fractionation of cytoplasmic and nuclear extracts and measurement of the amount of full product 36 hours after infection in parental (black bars) and 42-7 (grey bars) cells; (E) Parental and 42-7 cells...
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Engineering and characterization of human renal proximal tubular cells for applications in vitro toxicology and bioartifical kidneys
... Isolation of HPTC and characterization of isolated and commercial HPTC 50 4.1.1 Isolation of HPTC 50 4.1.2 Characterization of HPTC by immunoblotting 51 4.1.3 Characterization of ... containing 0.1 mM of 3-isobutyl-1-methylxanthine (IBMX) (Merck) and exposure of cells to 100 nM and 1000 nM of human PTH (Sigma-Aldrich) and human AVP (SigmaAldrich) for 30 minutes at 37°C The cells were ... (p-values of < 0.05) unless stated otherwise 49 Results 4.1 Isolation of HPTC and characterization of freshly isolated and commercial HPTC 4.1.1 Isolation of HPTC HPTC are available commercially and...
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Báo cáo khoa học: Isolation and characterization of an IgNAR variable domain specific for the human mitochondrial translocase receptor Tom70 potx
... Import and assembly of proteins into mitochondria of mammalian cells Biochim Biophys Acta 1592, 97–105 19 Suzuki, H., Maeda, M & Mihara, K (2002) Characterization of rat TOM70 as a receptor of the ... respectively, and the resultant VNARs evaluated for retention of binding affinity Material and methods Equipment and reagents Restriction enzymes and Vent DNA polymerase were purchased from New England ... ampicillin and/ or 2% (w/v) glucose Solid media contained 2% (w/v) Bacto-agar Transformation of E coli was by standard procedures [24] performed using electrocompetent cells Isolation of total RNA...
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Báo cáo khoa học: " Isolation and characterization of canine umbilical cord blood-derived mesenchymal stem cells" pps
... (Figs 4E and F) Discussion Isolation and characterization of stem cells derived from various tissues and sources have been one very critical issue for stem cell therapy [12,28,33] The purpose of this ... capacities Stem Cells Dev 2007, 16, 597-604 14 Igura K, Zhang X, Takahashi K, Mitsuru A, Yamaguchi S, Takashi TA Isolation and characterization of mesenchymal progenitor cells from chorionic villi of human ... Cotransplant of neural stem cells and NT-3 gene modified Schwann cells promote the recovery of transected spinal cord injury Spinal Cord 2007, 45, 15-24 12 Hipp J, Atala A Sources of stem cells for...
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Isolation and characterization of stem cell regulatory genes oct4 and stat3 from the model fish medaka
... potency of stem cells 1.1.2 Fundermental features of stem cells 1.1.3 Signalling pathways modulating pluripotency in stem cells 1.1.4 Transcription factors controlling pluripotency of stem cells ... unspecialized daughter cells and the potential of differentiation into specialized cells Stemness refers to the undifferentiated status of self-renewing stem cells Stem cells show a hierarchy of developmental ... mammalian systems xii Chapter I Introduction Chapter I Introduction 1.1 Stem cells 1.1.1 Developmental potency of stem cells Stem cells are unspecialized cells that have the capability of self-renewal...
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Isolation and characterization of the novel human gene, MOST 1
... cancers and prostate cancers, and is also the candidate site of potential oncogene(s) other than c-myc located at 8q24.1 Analysis of paired biopsies of invasive ductal breast cancer and adjacent ... study of isolation and characterization of a novel human gene was initiated The objectives of this study were as follow: To isolated full length cDNA; To analyze the genomic structure of MOST-1; ... protein in human cell lines namely hTERT-HME1 normal human mammary epithelial, MCF7 breast adenocarcinoma, PrEC normal human prostate epithelial and DU145 prostate carcinoma Aggregation of overexpressed...
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Isolation and characterization of an extracellular polymer closely related to flocculation of activated sludge
... polysaccharide One milligram of the partially purified polymer contained 309.7 µg of protein, 160.2 µg of neutral sugar, 58.5 µg of hexosamine, 30.8 µg of uronic acid and 3.8 µg of phosphorus Constituent ... concentration of ppm to estimate the effect of pH After min, A660 was measured and the extent of flocculation was calculated Pronase digestion of extracellular polymer Forty milligrams of the extracellular ... as follows: 500 g of soil was washed times with distilled water and suspended in 1,000 ml of water The mixture was allowed to stand for 10min and the supernatant was collected and used for the...
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Tài liệu Báo cáo khoa học: Isolation and characterization of four type 2 ribosome inactivating pulchellin isoforms from Abrus pulchellus seeds docx
... Nomenclature of the toxic pulchellin lectins The abbreviation P is followed by the Roman numerals I, II, III and IV and refers to each pulchellin Characterization of four pulchellin isoforms isoform ... III and P IV isoforms from the eluate P III ⁄ P IV (Fig 1C) Isoelectric focusing gave pI of 5.8, 5.7, 5.5 and 5.2 for the four isoforms respectively Secondary structure of the pulchellin isoforms ... mixture of other isoforms (B) Gel visualization of proteins eluted Lane 1, molecular weight markers Numbers on the left indicate the Mr values of the standards in thousands Lanes 2–9, lg of peak...
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Tài liệu Báo cáo khoa học: Phenol hydroxylase from Acinetobacter radioresistens S13 Isolation and characterization of the regulatory component docx
... of 50 nmÆmin)1, band-width of 1.0 nm, and a resolution of 0.1 nm in the temperature range between 10 °C and 70 °C, after preincubation for 10 at each temperature Three runs were accumulated and ... of 0.6 lM The data were fitted to Michaelis–Menten curves Squares and dotted line: data and fitting with PHI/PHO ratio of 0.5 Triangles and continuous line: data and fitting with PHI/PHO ratio of ... Asterisks and broken line: data and fitting with PHI/PHO ratio of hypothesis and therefore a direct involvement of PHI in electron transfer This conclusion is consistent with the very low degree of identity...
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Tài liệu Báo cáo khoa học: The isolation and characterization of cytochrome c nitrite reductase subunits (NrfA and NrfH) from Desulfovibrio desulfuricans ATCC 27774 Re-evaluation of the spectroscopic data and redox properties ppt
... intense band of 61 kDa (NrfA) and a band of weak intensity of 19 kDa (NrfH), confirming its hetero-oligomeric nature (Fig 1, lane 1) However, in the absence of boiling (Fig 1A, lanes and 4) high ... and 4) high molecular mass bands of approximately 110 kDa and > 200 kDa were visible, as well as a faint band at 37 kDa, suggesting the presence of dimers All of the bands stained positively for ... suggests that the bands at approximately 110 and 37 kDa correspond to strongly attached dimers of both NrfA (a2) and NrfH (b2), respectively The presence of dithiothreitol (Fig 1A, lanes and 2) did not...
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Báo cáo khoa học: Engineering and characterization of human manganese superoxide dismutase mutants with high activity and low product inhibition potx
... backbone amide and carbonyl of Cys140 form hydrogen bonds with the backbone carbonyl and amide of Trp123, the side chain of which forms one wall of the active site cavity and the replacement of which ... site-directed mutagenesis, and the parent Q143A hMnSOD and wild-type hMnSOD, are given in Table and Fig Catalysis of the decay of superoxide by the single mutants of Table all followed Michaelis ... the uncatalyzed dismutation of superoxide [32] A least-square fit of Eqn (1) to the data of Fig yields the constants given in the legend of Fig The values of kcat ⁄ Km and kcat for catalysis by...
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Báo cáo Y học: Isolation and characterization of a thioredoxin-dependent peroxidase from Chlamydomonas reinhardtii doc
... Chlamydomonas cells Chlamydomonas CW15 cells were grown to a cell density > ´ 106 cells per mL Cells were pelleted, resuspended in 30 mM Tris/HCl pH 7.9, and broken by two cycles of freeze-thawing ... RESULTS Cloning and sequences of Ch-Prx1 cDNA and the Ch-Prx1 gene In order to complete the sequence data for this new protein, and to be able to make a thorough characterization of its biochemical ... BLAST of peptide sequences of 21 kDa proteins in databases Tryptic peptides were puri®ed by RP-HPLC and some of them were totally or partially analyzed by Edman sequencing (ááá) and/ or by MALDI-TOF...
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Báo cáo khoa học: Isolation and characterization of a D-cysteine desulfhydrase protein from Arabidopsis thaliana pptx
... presequence of 19 amino acids The mature protein would have a molecular mass of 41.7 kDa and a pI of 6.34 The YedO protein from E coli and the d-CDes from Arabidopsis showed an overall identity of 36% and ... d-CDes mRNA and protein levels in older plants, during the light phase in a diurnal light ⁄ dark rhythm and under sulfate limitation Results In silico characterization and isolation of the Arabidopsis ... purity of mitochondria prepared from green tissue of tobacco, pea and Arabidopsis thaliana Phyton 43, 215–229 53 Leegood RC & Malkin R (1986) Isolation of sub-cellular photosynthetic systems In...
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Báo cáo khoa học:The isolation and characterization of temperature-dependent ricin A chain molecules in Saccharomyces cerevisiae docx
... strain genuinely reflects of the sensitivity of the mutant toxin to temperature To obtain a clearer picture of the growth profiles of yeast cells expressing these RTAs, cells were plated at various ... permissive temperatures of 30 °C and 37 °C, respectively A sample of the cells was removed from each culture, rRNAs were isolated in TRIzolÒ (Invitrogen, Paisley, Scotland), and the extent to which ... site molecules Y80, Y123 and E177 are shown in green and the position of the two mutated amino acids, F108 and L151, are shown in blue between the aromatic rings of Tyr80 and Tyr123 close to the...
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Báo cáo Y học: Isolation and characterization of MUC15, a novel cell membrane-associated mucin pot
... tonsil, and fetal liver Furthermore, PCR screening of bovine and human cDNA libraries showed the presence of MUC15 mRNA in human breast, bovine mammary gland, bovine lymph nodes and lungs of both ... Identification and cloning of the human MUC15 cDNA In order to investigate the existence of a human MUC15 homologue, the bovine MUC15 nucleotide sequence was employed in a search of the human genome ... database, and a similar sequence was located The milk cell fraction of lactating tissue contains bud-off epithelial cells, enabling performance of an indirect assay for expression of this possible human...
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Báo cáo khóa học: Isolation and characterization of the Xenopus HIVEP gene family ppt
... of degenerate and specific primers and by rescreening the cDNA libraries The partial clones of XHIVEP2 and -3 covered 5.9 and 3.2 kb of the respective 3¢ ends and included 3¢-UTRs and poly(A)-tails ... nucleus after transfection of COS cells [23,24], and the endogenous human HIVEP (PRDII-BF1) protein was detected in the nucleus of MG63 cells [6] Visual inspection of the full length XHIVEP1 ... -2 and -3) The Xenopus XHIVEPs are characterized with respect to temporal and spatial expression, nuclear import/export activity and DNA binding specificity Materials and methods Isolation and...
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Báo cáo hóa học: " Isolation and characterization of cidofovir resistant vaccinia viruses" pot
... streptomycin and non-essential amino acids Vero Cells were obtained from ATCC and were maintained in MEM with Earl's salts and the addition of 10% FBS and standard concentrations of L-glutamine, ... Domains of E9 and locations of mutations responsible for drug resistance Domains of E9 and locations of mutations responsible for drug resistance The location of the putative exonuclease and polymerase ... Mapping and marker rescue of recombinant viruses A Map of PCR fragments in the E9L region that were used for marker rescue mapping experiments and reconstruction of resistant viruses B Results of...
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Báo cáo hóa học: " Isolation and characterization of a new simian rotavirus, YK-1" docx
... the G and P serotype specificities of the YK-1 strain, the sequences of the genes encoding both VP7 and VP4 were determined and compared with those of representatives of established G and P serotypes ... Electrophoretic study of the genome of human rotaviruses from Rio de Janeiro, Sao Paulo and Belem, Brazil J of Hygiene 1983, 90:117-125 Palombo EA, Bishop RF: Genetic and antigenic characterization of a serotype ... rotavirus RNA segment 11, and (c) schematic diagram of the sequences of gene segment 11 Arrows indicate segment 11 of RRV and YK-1, or rearranged segment 11 of variant 311 The YK-1 and its variant clone...
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Báo cáo khoa học: " Isolation and characterization of Streptococcus sp.from diseased flounder (Paralichthys olivaceus )in Jeju Island" ppt
... eht fo esahp suoeuqa reppu eht no detcelloc saw AND lairetcaB nim rof 000,6 ta degufirtnec dna nim rof sllec lairetcab gniliob yb detcartxe neht saw AND lairetcab ;retaw dellitsid elbuod dezilirets ... 817( rof evitisop ,9~4 senal ;)pb 001,1( rof evitisop ,3 dna senal ;noitcefni laccocotperts rof evitagen ,1 enal ;lortnoc evitagen ,N enal ;)pb 003 ,M4040 ( lortnoc evitisop ,P senal ;reddal AND ... enilav ,esapil rof snoitcaer evitagen dah setalosi llA nispyrtomyhc -α dna esatahpsohp enilakla rof snoitcaer evitisop dah fo setalosi 81 dna esadinimasoculg-β-lyteca -N dna esaretse rof snoitcaer...
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Isolation and characterization of highly pathogenic avian influenza virus subtype H5N1 from donkeys docx
... of human erythrocytes were dispensed to Figure Phylogenetic analyses of HA and NA of an equine H5N1 isolate sequence in comparison to Egyptian human and avian isolates a, HA gene b, NA gene Human ... poultry increase exposure risks to surrounding humans and other mammals and in turn, create opportunities for the emergence of human- adapted strains with pandemic potential [19,20] Since 2006, H5N1 ... letters showed site of H274Y and N294S substitutions (H275Y and N295S in N1 influenza numbering) cough, fever and serous nasal discharge The course of the disease was short (72 h) and responsive well...
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Tìm thêm: xác định các mục tiêu của chương trình khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo gắn với các giáo trình cụ thể tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra đối với đối tượng giảng viên và đối tượng quản lí điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ lồng sóc mở máy động cơ rôto dây quấn các đặc tính của động cơ điện không đồng bộ đặc tuyến hiệu suất h fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến tốc độ rôto n fi p2 đặc tuyến dòng điện stato i1 fi p2 phần 3 giới thiệu nguyên liệu chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008 chỉ tiêu chất lượng 9 tr 25