... activesites of this enzyme and arginase I. In spite of the rel-atively low agmatinase activity of the Asn149Asp vari-ant, it is clear that the interactions of arginase II with l-arginine and agmatine ... variants. Interaction of arginase II with substrate and manganese ions V. Lo´pez et al.4542 FEBS Journal 272 (2005) 4540–4548 ª 2005 FEBSInsights into the interactionof human arginase II with substrate and ... examine the interactionof human arginase II (EC 3.5.3.1) with sub-strate and manganese ions, the His120Asn, His145Asn and Asn149Aspmutations were introduced separately. About 53% and 95% of wild-typearginase...
... various kinds of effects.In chapters 7, 8 and 9 we will explore several of these forms of interconnection of class and gender. Chapter 7 discusses the problem of the class location of married women ... way of formally representing the interactionof class and gender is with a simple equation of the sort used in multivariate regressionanalysis. Suppose we were studying the effects of class and ... important cause of everything social. Historical materialism is not atheory of all social phenomena, but only of a speci®c set of explananda ±the historical trajectory of economic structures and their...
... region within the d and e bands of collagen fibrils. Twochemical derivatives of collagens andof positive peptideswere prepared by N-acetylation and N-methylation of theprimary amino group of Lys/Hyl ... interact with fibrillar c ollagensthrough its protein core and to localize at d and e bands of the c ollagen fibril banding pattern. Using a s olid-phaseassay, we have determined the interactionof ... spectra of a recombinant decorin purified in theabsence of chaotropic agents, with the exception of thewavelength of the minimum (215–216 instead of 218 nm) and very different to the spectrum of...
... 5 mL of this, and incubatedfor 2 h at 24 °C. To remove the unbound antibodies, thebeads were washed twice with 5 mL of NaCl ⁄ Pi, twice with 5 mL of 100 mm glycine (pH 3.0), and twice with ... S3) [10,19]. Interactions of the truncatedTnrA proteins with GlnK andwith GS were determinedby pulldown and SPR analysis, as described above(Fig. 6). As expected, the C-terminus of TnrA was ... nucleotides,such as ADP, AMP, and GTP, at concentrations of Fig. 3. Influence of various effector molecules on the interaction of GlnK with the His6-TnrA surface. GlnK was preincubated with effec-tor molecules...
... amixture of annexin A1 and A2 and that the 35 kDa proteins are a mixture of annexin A5 and proteolytic fragments of annexin A1 and annex-in A2. Purified recombinant annexin A1, annexin A2 and annexin ... shown asthin dotted lines in (C) and (D). The result obtained in the presence of dicalcin and all of the annexins (E) was compared with the calcu-lated sum of each of the initial rates obtained ... localize in the cilia of frog olfactory and respiratory epithelium [7]. Tounderstand the possible association of annexin A1,annexin A2 and annexin A5 with the function of dical-cin, we examined...
... forhuman Vb5.2 and V b1withKd of 9–11 lM with a fast kass and a moderately fast kdiss. In spite of the reported stimu-lation of V b2.1 bearing T-cells by SSA, we o bserved nomeasurable interaction. Keywords: ... Consequently, the molecularstudies of the interactionof SAgs with their s pecifics ligandswill not only advance understanding of the physiologicalmechanisms of these molecules, but may lead to ... Recognit. 16,91–101.Ó FEBS 2004 Interactionof human TCR with superantigen SSA (Eur. J. Biochem. 271) 4083Cloning, expression andinteractionof human T-cell receptors with the bacterial superantigen...
... )*kon;1koff;1AB ð2ÞAB þ B )*kon;2koff;2AB2ð3Þwhered½B=dt ¼À2kon;1½A½Bþkoff;1½ABÀkon;2½AB[B]þ 2koff;2½AB2ð4Þd½AB=dt ¼ 2kon;1½A½BÀkoff;1½ABÀ kon;2½AB[B] þ 2koff;2½AB2ð5Þd½AB2=dt ... binding of factor X and its Gla-containing fragments to membranes containing 25 mol% PtdSer in the presence of 10 mMCa2+obtained by evaluation of association and dissociation phases (I) and equilibrium ... (1972) Mode of action of vitaminK and calcium binding properties of bovine prothrombin. Bio-chemistry 11, 4961–4964.24. Stenflo, J. & Ganot, P. (1973) Binding of Ca2+to normal and dicoumarol-induced...
... levels.Thus the drastic reduction in interactionof ND35 and ABFig. 8. Y2H interaction between MP and CP. (A) pGBK T7 (MP, MP deletion mutants and p53) and pGAD T7 (CP and T Ag) clones weretransformed ... similarity with MPs of viruses from other gen-era. Within sobemoviruses, the sequence of the SeMVMP was closest to that of the SBMV-Ark MP (32%sequence identity), and the identity with MPs of othersobemoviruses ... 4. Interactionof rMP with NV. (A) ELISA of rMP and NV inter-action. ELISA plates coated with NV (5 lg) (P1) were blocked with 10% skimmed milk in 1% NaCl ⁄ Pi (block) followed by addition of 5...
... correlate with the binding affinity between GA and the receptor.The thermodynamic parameters obtained are in therange of those of other interactions with c-CD [15].The interactionof GA with c-CD ... with the interaction of GA and c-CD [21,22]. In addition, theDCpvalue, )0.14 kcalÆmol)1Æ°C)1, is large and negat-ive, which is in the largest range among those of otherinteractions with ... glycer-aldehyde-3-phosphate dehydrogenase and induce asmearing of its electrophoretic band and dephosphoryla-tion. FEBS Lett 579, 4333–4336. Interaction of gymnemic acid with cyclodextrins Y. Izutani et...
... dose of drug delivered depends on the surface area of the patch and the length of time that the patch is worn,which allows for tailoring the duration of medicationeffect with reductions of first-pass ... percentages of caregiv-ers were satisfied with the behavioral efficacy of MTS aswell. Consistent with the commonly reported TEAEs of decreased appetite and insomnia, the majority of caregiv-ers ... mood and substance use disorders [8-11],children with ADHD struggle with impairment in aca-demic functioning, self-esteem, and interpersonal rela-tionships [12,13]. Families of children with...
... endoscopic and radiographic. Asymptomatic colonic and gastric carcinoma may present with IDA and exclusion of these conditions is of prime concern. The upper endoscopic evaluation should include random ... common cause of anemia and affects about 5–12% of non-pregnant women and 1–5% of men have IDA [1-2]. It is a result of blood loss from the gastrointes-tinal tract or the uterus and is a requiring ... presence of dyspeptic com-plaint and its severity calculated by presence of ab -dominal pain, abdominal pain with hungry and an -xiety, abdominal distension, nausea, vomiting, poor appetite and...
... in ischemic stroke patients with APS. The subjects were 20 ischemic stroke patients with antiphospholipid antibody, 13 with pri-mary antiphospholipid syndrome and 7 with SLE-related antiphospholipid ... deal-ing with the combination of antiplatelet and antico-agulant therapy for ischemic stroke patients with APS based on the strict Sydney criteria [5]. One reason may be that patients with stroke ... that patients with stroke complicated with APS are rather rare compared with patients with uncompli-cated stroke, and this is also the reason why the number of patients in this study was quite...