... spectra of a recombinant decorin purified in the
absence of chaotropic agents, with the exception of the
wavelength of the minimum (215–216 instead of 218 nm)
and very different to the spectrum of ... 2002 Interactionof decorin with collagen peptides (Eur. J. Biochem. 269) 1431
with respect to the unmodified ones is not related to the
percentage of Lys/Hyl side chains that did not react with ... these data demonstrate the essential role
of the positive charge of collagen Lys/Hyl residues for
interaction with decorin.
DISCUSSION
The binding of decorin with fibrillar collagens has been
extensively...
... for
the interactionof frog annexin A1 and annexin A2
with dicalcin. Consistently, we observed that the
35 kDa forms of annexin A1 and annexin A2 found
in the fraction of the binding proteins of dicalcin
(Fig. ... presence
of dicalcin and all of the annexins (E) was compared with the calcu-
lated sum of each of the initial rates obtained in (B)–(D) (thick dot-
ted lines).
T. Uebi et al. Role of dicalcin ... 4869
Comprehensive interactionof dicalcin with annexins
in frog olfactory and respiratory cilia
Tatsuya Uebi
1
, Naofumi Miwa
1,2,
* and Satoru Kawamura
1,2
1 Department of Biology, Graduate School of Science,...
... into the interactionof human arginase II with
substrate and manganese ions by site-directed
mutagenesis and kinetic studies
Alteration of substrate specificity by replacement of Asn149
with Asp
Vasthi ... spite of the rel-
atively low agmatinase activity of the Asn149Asp vari-
ant, it is clear that the interactions of arginase II with
l-arginine and agmatine are greatly altered by replace-
ment of ... blue
staining of purified enzymes.
Metal-free species of purified enzymes were obtained by
incubation for 1 h at 25 °C with 25 mm EDTA and 3 m
V. Lo
´
pez et al. Interactionof arginase II with substrate...
... intensity of
the band of unhydrolyzed actin was plotted against the time
of incubation.
Characterization of actin preparation quality
Fluorescence parameters were used for estimation of
nativity of ... aggregation of partially
folded actin.
To obtain more detailed information on the interaction of
HSP25 with partially unfolded actin, we analyzed the
effect of different quantities of HSP25 on ... parameter A of actin was close to
2.4. Increase of the time of heating at 43 °C leading to
decrease of parameter A up to 2.2–2.3 was accompanied by
further decrease of the initial rate of polymerization....
... basal
levels of resonance units (GlnK free surface) within
seconds. To test the effects of various molecules on the
interaction of TnrA with GlnK, 40 nm GlnK (trimer)
Fig. 1. Coimmunoprecipitation of TnrA, ... equilibrated with 10 column volumes
(10 · 0.2 mL) of buffer B, with subsequent washing four
times with five volumes of the same buffer. Proteins were
eluted with buffer E (buffer B supplemented with 250 ... (SPR) of the
GlnK–TnrA interaction
As a next step, the interactionof TnrA with GlnK was
investigated in vitro by BIAcore SPR detection. For
this analysis, a Strep-tag II-tagged variant of GlnK
(GlnK-ST)...
... Recognit. 16,
91–101.
Ó FEBS 2004 Interactionof human TCR with superantigen SSA (Eur. J. Biochem. 271) 4083
Cloning, expression and interactionof human T-cell receptors
with the bacterial superantigen ... Vb5.2 and V b1withK
d
of 9–11 l
M
with a fast k
ass
and a moderately fast k
diss
. In spite of the reported stimu-
lation of V b2.1 bearing T-cells by SSA, we o bserved no
measurable interaction.
Keywords: ... Consequently, the molecular
studies of the interactionof SAgs with their s pecifics ligands
will not only advance understanding of the physiological
mechanisms of these molecules, but may lead to...
... report on the interactionof an
N-terminal (E2(7–26)) and an internal (E2(279–298))
synthetic peptide sequence of the E2 structural pro-
tein of HGV ⁄ GBV-C with phospholipid membranes
of different ... short hydrophobic segments of viral envelope glyco-
proteins with a very low content of hydrophilic amino
acids, the presence of acidic residues in the fusion pep-
tides of some low-pH-activated ... strongly interacted
with PtdCho ⁄ PtdSer (65 ⁄ 35) vesicles, as the blue shift
of Trp was 11 nm.
To study the contribution of electrostatic inter-
actions to the binding of both peptides with negatively
charged...
... study, we measured the interactionof stefin B
with various combinations of phospholipid monolayers
and bilayers. Interactionof stefin B in the prefibrillar
aggregated state with model lipid membranes ... channel hypothesis of AD and neurodegeneration
in general, is not incompatible with other key elements
of toxicity, as, for example, the deregulation of Ca
2+
homeostasis and generation of reactive ... membranes, initiating a cascade of detrimental
events for the cell. Interactionof granular aggregates and globular oligo-
mers of an amyloidogenic protein, human stefin B, with model lipid mem-
branes...
... predicted protein
is composed of 306 amino acids, with a calculated molecular
mass of 34.3 kDa and a pI of 5.72. Similar searches with the
deduced amino-acid sequence of f ull-length Calliphora
AFP, ... according to the following scheme: 3 · 10 min with
4 · NaCl/Cit; 2 · 10minwith2· NaCl/Cit; 1 0 min
with 0.1 · NaCl/Cit; 10 min with 0.05 · NaCl/Cit; 5 min
with NaCl/Tris. After incubation for 30 m ... encoded a protein
with 93% identity in the deduced amino-acid sequence with
the A FP of S. peregrina (GenBank accession number
BAA99282). Because of the high sequence identity with
the Sarcophaga...
... )
*
k
on;1
k
off;1
AB ð2Þ
AB þ B )
*
k
on;2
k
off;2
AB
2
ð3Þ
where
d½B=dt ¼À2k
on;1
½A½Bþk
off;1
½ABÀk
on;2
½AB[B]
þ 2k
off;2
½AB
2
ð4Þ
d½AB=dt ¼ 2k
on;1
½A½BÀk
off;1
½AB
À k
on;2
½AB[B] þ 2k
off;2
½AB
2
ð5Þ
d½AB
2
=dt ... that mutation of Ca
2+
ligating
amino acids in the N-terminal part of the first EGF-like
domain of factor X influences the amidolytic activity of the
intact protein [36]. However, direct interactions ... vicinity of the membrane.
We found that the isolated factor X Gla domain exhibits
low affinity binding to PtdSer-containing membranes with a
K
d
of 4.6 l
M
. This agrees well with the value of 2.4...
... behavior of trehalase
depending on the nature of the activation stress (Fig. 5B).
This shift might argue against the participation of a unique
set of interactions involving association of Ntp1p ... identification of strains C3 and
C69, with one copy of Ntp1p–Ha6H expressed from the
genomic ntp1
+
promoter, was verified by Southern blot
analysis and immunoblot of whole-cell extracts with anti-
Ha ... the regulation of Ntp1p activity under stress relies
on the existence of some kind ofinteraction between Ntp1p
and Tps1p, or other elements, that may be lost under the
conditions of the in vitro...
... sequence similarity
with MPs of other genera, but showed 32% identity with the MP of South-
ern bean mosaic virus within the Sobemovirus genus. With a view to
understanding the mechanism of cell-to-cell ... similarity with MPs of viruses from other gen-
era. Within sobemoviruses, the sequence of the SeMV
MP was closest to that of the SBMV-Ark MP (32%
sequence identity), and the identity with MPs of other
sobemoviruses ... in
Fig. 8A,B). These results show that the interaction of
A
B
Fig. 7. Mapping of the SeMV MP domain responsible for interaction
of MP with NV. (A) Purification of GST–MP deletion mutants. GST–MP
deletion...
... dissociation
was observed with the A
8
B
8
isoform of GAPDH lead-
ing to the A
2
B
2
isoform with a higher NADPH-
dependent activity [39].
By analogy with the CTE of the isoform of GAPDH
[15,37], we ... date, mapping of the interaction site of CP12
had not studied but our results give some clues with
regard to the binding of CP12 to GAPDH. The impact
of CP12 binding on the activity of GAPDH is ... dissociation results
in the release of PRK as a dimer, whereas GAPDH
remains strongly associated with CP12 and forms a
subcomplex of one tetramer of GAPDH with one
monomer of CP12 [2,10]. However, in...
... were
washed twice with buffer C and twice with buffer C with-
out NP40. The elution was made with three volumes of
buffer C without NP40 adjusted to 1.5 m NaCl, incubating
for 10 min with gentle stirring. ... nuclear-enriched fraction of proteins. The effi-
ciency of labelling of the single oligomers being not
homogenous, the ratios of the different binding signals
within each lane instead of their absolute ... the amount of recovered protein owing to
the presence of a high molar excess of BSA remaining in
the recovery buffer. Therefore, a fixed aliquot of the protein
was incubated with 1 ng of the indicated...