in early b cells
Báo cáo khoa học: Prohibitin is expressed in pancreatic b-cells and protects against oxidative and proapoptotic effects of ethanol pdf
... (nuclei) staining; (B) Mitofluor Red 589 (mitochondria) staining; (C) anti-PHB ⁄ anti-rabbit-FITC staining; (D) merge Arrows indicate staining of PHB D proteins have been shown to be involved in < /b> endocytosis ... 2009 FEBS 491 Effects of prohibitin in < /b> b- cells G1 G2 J H Lee et al G2E G2P G2EP A B C Fig Localization of exogenous PHB in < /b> RINm5F cells RINm5F cells were incubated for 24 h as described in < /b> Fig ... data indicate that exogenous PHB enters the cells PHB is expressed in < /b> b- cells and increased by ethanol To determine the expression of PHB in < /b> b- cells we first analyzed the PHB protein level by western...
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Báo cáo y học: "Analysis of the 5''''UTR of HCV genotype 3 grown in vitro in human B cells, T cells, and macrophages" potx
... human macrophages; (B) HCV obtained from the macrophages was then used to infect freshly transformed B- cells or T -cells obtained from human fetal cord blood and cultured in < /b> the presence of 100 ... particular individual may harbor different genotypes of HCV [22-24], suggesting that cell tropism may establish the tissue specificity of HCV in < /b> infected individuals Variability in < /b> diseases of HCV-infected ... doubly infected with HIV and HCV The HCV was genotyped as type using an INNO-LiPA assay by a clinical testing laboratory (Quest Diagnostics) In < /b> vitro culture system Our culture system, described...
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Somatostatin induced SSTR2A endocytosis is regulated through b arrestin2 arf6 EFA6A PLD1 cascade in panceratic b cells (RINm5F)
... Total binding of 125I-SS to RINm5F cells The highest total binding was observed at incubation of the tracer When we increased the incubation time, the total binding was decreased At 90 min, the binding ... Arf GAP, Arf GAP domain; ANK, ankyrin repeat; BAR, Bin/Amphiphysin/Rvs; BoCCS, binding of coatomer, cargo and SNARE; CALM, CALM binding domain; CB, clathrin-box; CC, coiled-coil; FG repeats, multiple ... 0.1M PBS and 3X of 50mM KPBS washes, blocking buffer (KPBS buffer containing 1% BSA, 0.4% Triton-X-100, 1.5% FBS) was added for h before adding 1st Ab overnight The protein expression of constructs...
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Báo cáo khoa học: Activation of activating transcription factor 2 by p38 MAP kinase during apoptosis induced by human amylin in cultured pancreatic b-cells ppt
... specific MAPK inhibitor alone (SB203580, JNK inhibitor I or PD98059), or combinations of inhibitors (SB203580 + JNK inhibitor I) or (SB203580 + JNK inhibitor I + caspase-8 inhibitor) or inhibitor-negative ... DNAbinding complexes were detected ATF-2-CRE DNA binding activity was markedly induced following 8-h hA treatment, as shown by increased intensities of both shifted bands in < /b> hA-treated cells In < /b> ... In < /b> addition, the increased CRE binding activity was suppressed by JNK inhibitor I and SB 203580, implying that hA-evoked CRE binding is mediated by both the JNK and the p38 kinase pathways Additionally,...
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Báo cáo Y học: RING finger, B-box, and coiled-coil (RBCC) protein expression in branchial epithelial cells of Japanese eel, Anguilla japonica pot
... MG-132, and 100 ng of GSt-eRBCC E2s (UbcH2, UbcH5C, UbcH7, UbcH8, and UbcH9) used in < /b> ubiquitination assay were expressed as recombinant proteins in < /b> E coli After incubation at 30 °C for h, the ... analysis of eRBCC protein expressed in < /b> COS-7 cells COS-7 cells expressing eRBCC or mock transfected cells were solubilized with the Laemmli buffer and analyzed by Western blotting as described under ... a nylon membrane, and hybridized with eRBCC 32P-labeled cDNA probe Position of 2.6 kb and 1.8 kb are as noted in < /b> figure Hybridization to an eel b- actin probe demonstrated equal loading of the...
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Pulmonary tuberculosis associated with increased number and percentage of natural killer and B cells in the peripheral blood pot
... peripheral blood of patients with PTB A variety of immune abnormalities of the peripheral blood lymphocyte subsets including NK cells in < /b> PTB have already been described in < /b> PTB (Snyder et al., 2007; Barcelos ... role of B cells in < /b> the optimal host response against MTB by increasing the infection inoculum (Vordermeier et al., 1996; Maglione et al., 2007) In < /b> addition the protection of mice against MTB infection ... detected in < /b> this study The most notable findings were increased percentage with an absolute numbers of NK and B cells while reduced percentage with an absolute numbers of CD4 + lymphocytes in < /b> the...
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Báo cáo khoa học: Lending a helping hand, screening chemical libraries for compounds that enhance b-hexosaminidase A activity in GM2 gangliosidosis cells pptx
... that the destabilizing effects of some mutations may be offset by the stabilizing effects of a bound substrate or inhibitor (i.e the PC) In < /b> theory, PCs for LSDs should be displaced by the high ... Screening libraries for hexosaminidase enhancers (Fig 2A, B) This compound increased (two-fold) the levels of the mutant protein in < /b> Sandhoff cells bearing the bP504S mutation, as well as in < /b> ISD cells ... resulting compounds examined in < /b> detail act as competitive inhibitors (i.e they compete with the substrate for binding in < /b> the active sites in < /b> Hex [30] Although the PCs were indirectly identified using...
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Báo cáo y học: "The VH gene repertoire of splenic B cells and somatic hypermutation in systemic lupus erythematosus" potx
... germinal centre (GC) A (b) Staining for CD20 FDC staining suggests two possible GCs within close proximity, but B- cell staining shows no clear demarcation (c) Anti-FDC staining for GC B (d) staining ... distinct areas of staining for FDC and one large area of B cells encompassing both FDC regions Very few T cells could be seen within GC A and B, and no plasma cells could be seen at all within ... clones of B cells are proliferating and mutating in < /b> the splenic GCs and B- cell clusters Serine codon usage There is evidence for a bias toward serine codon usage within immunoglobulin variable genes...
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Báo cáo y học: "B cells as a therapeutic target in autoimmune disease" doc
... further increasing the antibody’s depleting activity [7] Rituximab has been used in < /b> the treatment of B- cell non-Hodgkin’s lymphoma as a single agent as well as in < /b> combination therapy, emphasizing ... cells being spared B- cell depletion in < /b> antibody-mediated diseases It is understandable that rituximab has been most frequently explored in < /b> autoimmune cytopenias, a disease group that is clearly linked ... CXCL13 have been identified, including endothelial cells and synovial fibroblasts Lymphotoxin-β was typically found on mantle-zone B cells In < /b> such synovial tissues, the T-cell responses are B- celldependent...
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Báo cáo y học: "Collagen type II (CII)-specific antibodies induce arthritis in the absence of T or B cells but the arthritis progression is enhanced by CII-reactive T cells" ppsx
... Collagen-antibody-induced arthritis in < /b> mice deficient in < /b> B cells and T cells Frequency (a) and severity (b) of arthritis in < /b> groups (n = 6–14) of B1 0.Q mice deficient in < /b> B cells (B KO), T cells (T KO), in < /b> ... Ab in < /b> MMC- Ab+TCL in < /b> MMC+ Ab+TCL in < /b> MMC- TCL in < /b> MMC+ TCL in < /b> MMC- Discussion 60 * *** *** 50 ** % of incidence 40 30 20 10 0 (b) 10 13 16 Days Ab in < /b> MMC+ Ab+TCL in < /b> MMC+ 19 22 26 30 33 38 Ab in < /b> ... unexpected finding because CAIA is induced in < /b> naive mice by preformed anti-CII antibodies Interestingly, however, immune cells might have a regulatory role in < /b> CAIA because mice deficient in < /b> both T cells...
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Báo cáo y học: "Targeting Toll-like receptor signaling in plasmacytoid dendritic cells and autoreactive B cells as a therapy for lupus" pps
... strings, which were noninhibitory [85] We now call these inhibitors class B INHODNs (B for ‘broadly reactive’), because they block TLR9mediated activation in < /b> all TLR9-expressing cells In < /b> B cells, ... signal-regulated kinase (ERK) phosphorylation in < /b> B cells, immune complexes containing chromatin were capable of inducing ERK activation in < /b> B cells They also stimulated IFN-α production by pDCs [58] ... the original observation that certain poly-G ODNs containing the nuclease-resistant PS backbone, but not those synthesized with the PO backbone, could block the production of IFN-γ induced by mitogens...
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Báo cáo y học: "Targeting B cells in systemic lupus erythematosus: not just déjà vu all over again" pdf
... which will probably be required as is true of rituximab [1] It is unfortunate that there is only limited preclinical information available on the signaling mediated by epratuzumab binding to CD22 ... is that combining epratuzumab with rituximab, although not increasing the overall clinical response rate above what is seen with rituximab alone, may lead to a substantially higher number of complete ... rates in < /b> B cell lymphomas than the agent alone [10] Such approaches also create more clinical adverse reactions and would probably not be acceptable for use in < /b> SLE Another curious finding in < /b> the...
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Báo cáo y học: "B cells in Sjögren’s syndrome: indications for disturbed selection and differentiation in ectopic lymphoid tissue" doc
... cytidine deaminase [114], they most probably represent altered CD27+ memory B cells but not GC B cells Thus, no circulating CD38++ IgD– GC B cells have been detected in < /b> the peripheral blood by ... J, Bjorgan MB, Thoen JE, Natvig JB, Thompson KM: Bm1-bm5 classification of peripheral blood B cells reveals circulating germinal center founder cells in < /b> healthy individuals and disturbance in < /b> ... expressed by B cells was also reported to be sufficient for Bcell differentiation, raising the possibility that B cells may be able to regulate themselves by CD27–CD70 interactions [72] Analysis by...
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Báo cáo y học: "Class-switched B cells display response to therapeutic B-cell depletion in rheumatoid arthritis" docx
... for subsequent clinical response Peripheral blood reconstitution The median number of B cells increased continuously from months to 12 This PB B- cell repletion was dominated by 'naïve' B cells ... (MemB) are in < /b> red, CD27-IgD- B cells are in < /b> light blue, and switched CD27+IgD- MemB are in < /b> blue (b) Line diagram of switched MemB in < /b> good responders (continuous line) and in < /b> non-responders or moderate ... to a clinical flare to be useful in < /b> clinical practice requires ongoing research in < /b> a prospective setting Competing interests PB B cells started to repopulate in < /b> all of our patients during the...
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Báo cáo y học: "Alterations in peripheral blood memory B cells in patients with active rheumatoid arthritis are dependent on the action of tumour necrosis factor" potx
... with infliximab, (that specifically blocks TNF) we have found an increase in < /b> circulating pre-switch memory B cells It is difficult to be certain whether the increase in < /b> pre-switch memory B cells ... showing the difference in < /b> CD27 expression in < /b> peripheral blood and synovial CD19+ B cells Dashed line shows staining with the isotype control and the solid line for CD27 (b) Box-plots representing ... B cells within inflamed tissue may contribute to a decrease in < /b> this B cell subset in < /b> the blood It should be pointed out that postswitch IgD-CD27+ memory B cells are also enriched in < /b> the rheumatoid...
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Báo cáo y học: "B cells in autoimmunity" ppsx
... Schematic B- cell development of B2 B cells emigrating as immature B cells from the bone marrow and differentiating further into naïve/marginal zone (MZ) B cells in < /b> the spleen, which subsequently ... presentation by B cells, in < /b> particular by memory B cells, may be important in < /b> the amplification and maintenance of autoimmunity after it has been initiated Memory B- cell subsets in < /b> SLE [46] provide ... pre -B cells The humanized anti-CD22 monoclonal antibody epratuzumab causes partial B- cell reduction in < /b> the blood, inhibits B- cell proliferation, and probably interferes with intracellular signaling...
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Báo cáo y học: "Rituximab therapy reduces activated B cells in both the peripheral blood and bone marrow of patients with rheumatoid arthritis: depletion of memory B cells correlates with clinical response" potx
... rituximab, an increase in < /b> PB memory B cells was observed (from 23 ± 8% to 39 ± 13%) Interestingly, similar changes were found in < /b> BM CD19+CD27+ memory B cells; these B cells decreased by 26 ± 10% in < /b> ... rituximab on B cells in < /b> rheumatoid arthritis peripheral blood and bone marrow Rituximab preferentially depletes B cells in < /b> peripheral blood marrow (PB) and reduces activated B cells in < /b> the PB and ... between depletion of B cells in < /b> PB or in < /b> BM and the clinical response to rituximab Taken together, these results suggest that the BM B cells are more resistant than PB B cells to antiCD20 mAb...
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Báo cáo y học: "Anti-tumour necrosis factor therapy and B cells in rheumatoid arthritis" pptx
... of B cells in < /b> the synovia, thereby increasing their proportion in < /b> the peripheral blood However, both pre- and post-switch memory B cells can be found in < /b> the synovia and no changes were seen in < /b> ... Steiniger B, Staudt LM, Casanova JL, Reynaud CA, Weill JC: Human blood IgM “memory” B cells are circulating splenic marginal zone B cells harboring a prediversified immunoglobulin repertoire Blood ... antibodies may be able to lyse cells that express TNF on their surface Etanercept, the receptor fusion protein, can bind not only TNF but also LTα Nevertheless, both treatment with infliximab and...
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Báo cáo y học: "Chemokine receptor expression and functional effects of chemokines on B cells: implication in the pathogenesis of rheumatoid arthritis" ppt
... B cells in < /b> RA has not been completely delineated In < /b> this study, we examined chemokine receptor expression by peripheral blood in < /b> both normal donors and subjects with RA, and also synovial B cells ... than 95% To block CCR7, B cells were incubated with μg/ml anti-CCR7 mAb (150503; R&D Systems, Minneapolis, MN, USA) or control mAb for 30 minutes Then, the × 105 B cells were incubated in < /b> 96-well ... inflammatory cells, including T cells, macrophages, dendritic cells and B cells [3-6] Synovial B cells could present antigens to T cells Importantly, rheumatoid factor-expressing B cells that are...
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báo cáo khoa học: "Identification of circulating tumour cells in early stage breast cancer patients using multi marker immunobead RT-PCR" doc
... cells in < /b> blood samples obtained from 56 early < /b> stage breast cancer patients Circulating epithelial cells were isolated from blood samples based on their ability to bind to the immunobeads via the BerEP4 ... 10 cells per ml of blood (Figure 1) In < /b> samples containing cell per ml of blood (10 cells total), marker expression was detected in < /b> 2/3 samples indicating some loss of cells during the immunobead ... epithelial cells that might be in < /b> the blood The justifiable assumption is that the only epithelial cells in < /b> blood or bone marrow are carcinoma cells A magnet can then be used to harvest these cells...
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