important fragments of activity and inactivity
Báo cáo khoa học: The N-terminal hybrid binding domain of RNase HI from Thermotoga maritima is important for substrate binding and Mg2+-dependent activity pot
... far- and near-UV CD spectra of Tma-RNase HI, Tma-CD and Tma-ND were measured at 20 °C and pH 9.0, and comparisons are shown in Fig The farand near-UV CD spectra of Tma-CD are similar to those of ... presence of mm MgCl2 and 0.1–5 mm MnCl2 (Fig 5) Both proteins exhibited little activity (less than 0.01% of the maximal activity) in the presence of NiCl2, ZnCl2, CoCl2 or CaCl2 The maximal Mg2+- and ... the presence of mm MgCl2 or MnCl2 and compared with those of Tma-RNase HI (Table 1) The Vmax values of Tma-CD determined in the presence of mm MgCl2 and MnCl2 were 410-fold lower and 1.6-fold...
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Báo cáo khoa học: Increased amylosucrase activity and specificity, and identification of regions important for activity, specificity and stability through molecular evolution doc
... measuring the standard activity at different temperatures Standard activity is determined by incubating the enzyme with sucrose and glycogen at final concentrations of 146 mm and 0.1 gÆL)1, respectively ... N-terminal domain and a B¢ domain between b strand and a helix in the catalytic core, which has been suggested to be involved in the polymerase activity of this enzyme The B and B¢ domains contribute ... concentrations (Vmax1 and Km1) and low affinity and high(er) Vmax at high sucrose concentrations (Vmax2 and Km2) [13] In Table the kinetic data Table Kinetics of the action on sucrose of (variant) enzymes...
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Báo cáo y học: "Pravastatin Provides Antioxidant Activity and Protection of Erythrocytes Loaded Primaqe"
... fragility and oxidative damage of erythrocytes of zinc-deficient rats J Nutr 1997; 127: 1290–1296 Lajos N, Miki N, Sandor S Protein and non-protein sulfhydryls and disulfides in gastric mucosa and ... spectrophotometric method [20] A mixture of 200 μL of 8% sodium dodecyl sulfate, 200 μL of 0.9% thiobarbituric acid and 1.5 ml 20% acetic acid was prepared 200 μL of RBCs lysate and 1.9 ml distilled water ... the volume of ml After boiling for h, the mixture was cooled, and ml of n-butanol and pyridine (15:1) solution was added to it This mixture was then centrifuged at 5000 rpm for 15 and the absorbance...
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Tài liệu Time trends in leisure time physical activity and physical fitness in elderly people: 20 year followup of the Spanish population national health survey (1987-2006) docx
... proportional random selection of primary and secondary sampling units (towns and sections, respectively), with the final units (individuals) being selected by means of random routes and sex- and age-based ... can help to prevent physical inactivity and improve the health status of older people in Spain List of abbreviations PA: Physical activity; LTPA: Leisure time physical activity; SNHS: The Spanish ... contributions DPC conceived of the study, and participated in its design and coordination and draft the manuscript CAB carried out the acquisition of the data, analysis and interpretation of data She has...
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Tài liệu Báo cáo khoa học: Secondary substrate binding strongly affects activity and binding affinity of Bacillus subtilis and Aspergillus niger GH11 xylanases docx
... of genetic engineering of the secondary binding site of the B subtilis and A niger xylanases on the screening ratio The screening ratio is defined as the ratio of activity on Xylazyme AX and activity ... NMR-monitored titrations of Bacillus circulans xylanase [13] and X-ray analysis of crystals of catalytically incompetent mutants of the xylanases of Bacillus subtilis (XBS) and Aspergillus niger ... reflected by the activity measurements Unexpectedly, Kd values of D32A are higher than those of the wild-type, whereas the activity of the mutant was unaffected Comparison of XBS and XAN In general,...
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Tài liệu Báo cáo khoa học: Processing, catalytic activity and crystal structures of kumamolisin-As with an engineered active site pptx
... pH 7.0 and °C for h, followed by SDS ⁄ PAGE The rate of ligation was estimated from percentage intensity of the band of the 57-kDa protein in a total of intensities of the 57-kDa, 38-kDa and 19-kDa ... at pH 7.0 and °C for indicated periods of time Lane c, the nicked precursor was incubated at pH 7.0 and °C for 24 h and then dialyzed overnight at °C and pH 4.0 (B) Effects of addition of different ... different amounts of the 38-kDa mature form (s) and the wild-type enzyme (d) on the rate of ligation Catalytic and substoichiometric amounts (molar ratio of 0.01 and 0.1, respectively) of these proteins...
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Tài liệu Báo cáo khoa học: Mutational analysis of plasminogen activator inhibitor-1 Interactions of a-helix F and its neighbouring structural elements regulates the activity and the rate of latency transition pdf
... behaviour and a low unstable inhibitory activity, and for I137A a biphasic loss of activity (Fig and Table 3) The phenotype of these substitutions is therefore different from that of N139A, T146A and ... are indicated in green, a-helix F in orange and parts of b-sheet A in pink Backbone atoms of the s1A/hFloop (residues D127 to E130) and of the top of hF and the hF/s3Aloop (residues T146 to L154) ... addition of 25 lL 0.3 mgÆmL)1 S-2444 (Chromogenix, Sweden) and further incubation for 40 The specific inhibitory activity of PAI-1 at the various timepoints, i.e the fraction of the total amount of...
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Tài liệu Báo cáo khoa học: Endotoxic activity and chemical structure of lipopolysaccharides from Chlamydia trachomatis serotypes E and L2 and Chlamydophila psittaci 6BC pdf
... a ratio of : : 0.01 (v/v/v) and sprayed at a flow rate of lLÆmin)1 MALDI-TOF MS of LPS and free lipid A were performed on a Reflex III (Bruker-Daltonik, Bremen, Germany) in the linear TOF configuration ... from C trachomatis serotype E and L2 were of similar activity and both less active than smooth LPS by a factor of 100 whereas, LPS from Chl psittaci was of lower activity than the two other chlamydial ... complex pattern of several groups of ion peaks representing molecular ions (centered around m/z 2510, 2300 and 2005) and of laser-induced fragment ions [18] representing the lipid A moiety of LPS (centered...
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Tài liệu Báo cáo khoa học: Adaptive changes in the expression of nuclear and mitochondrial encoded subunits of cytochrome c oxidase and the catalytic activity during hypoxia pptx
... switch and alters the expression of the two nuclear genome coded isoforms of CytOX V [10,32] The regulation of genes CytOX5a and CytOX5b, coding for the two isofoms Va and Vb, parallels that of genes ... Effect of hypoxia on CytOX activity, mitochondrial heme aa3 content and TN (s)1) in macrophages and PC12 cells CytOX activity in submitochondrial particles (SMP) from standard (141 Torr O2) and ... stages of adaptive response to hypoxia Based on the results of our study and those of Schumacker, it is reasonable to conclude that the effects of hypoxia on CytOX gene expression and its activity...
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Báo cáo khoa học: The oleic acid complexes of proteolytic fragments of a-lactalbumin display apoptotic activity pdf
... concentration (CAC)] of OA and induce the formation of small and water-soluble OA aggregates All OA complexes displayed apoptotic activity for tumor cells, and the extent of their activity was comparable ... Fig S1 Top: far-UV CD spectra of fragments 1–40 and 104–123 in the presence of increasing amounts of OA Bottom: cytotoxicity of the OA complexes of peptides 1–40 and 104–123 This supplementary ... properties in the presence of a-LA or its fragments With a decrease in the CAC of the fatty acid in the presence of the protein or its fragments, soluble and smaller aggregates of protein–OA or fragment–OA...
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Báo cáo khoa học: N-Glycosylation is important for the correct intracellular localization of HFE and its ability to decrease cell surface transferrin binding pptx
... end of an a-helical region of the HFE a1 domain (V100 and W103A) as being of particular importance for TfR1 interactions [16,17,20] The effect of HFE binding to TfR1 is to lower the affinity of ... sites of HFE N-glycosylation and examine the importance of glycosylation on parameters of protein localization and function Results Tunicamycin treatment results in a reticular pattern of HFE ... mutant forms of HFE and incubated the cells in the presence and absence of tunicamycin Drug treatment resulted in the accumulation of an unglycosylated lower molecular mass form of the wild-type...
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Báo cáo khoa học: Relationship between functional activity and protein stability in the presence of all classes of stabilizing osmolytes ppt
... on their effects on stability and activity parameters of RNase-A Functional stability and activity by osmolytes Results and Discussion Protein stability and enzyme activity have a well-correlated ... stability and activity changes in the presence of a wide range of osmolytes For this we evaluated the protein stability (DGD°, Gibbs free energy change at 25 °C) of RNase-A and its activity parameters ... advances in understanding the effect of osmolytes on protein stability, folding and the activity of proteins and enzymes, the relationship between protein stabilization by osmolytes and its consequent...
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Báo cáo khoa học: Haptoglobin binds the antiatherogenic protein apolipoprotein E – impairment of apolipoprotein E stimulation of both lecithin:cholesterol acyltransferase activity and cholesterol uptake by hepatocytes pdf
... polyacrylamide gel in denaturing and reducing conditions, and by western blotting (A) Coomassie-stained bands of isolated Hpt (lane 1), standard ApoE (lane 2), standard ApoA-I (lane 3), and partially purified ... HRP-conjugated avidin and ECL Coomassiestained bands of VLDL and LDL proteins are shown in lanes and 2, respectively VLDL and LDL proteins, blotted onto the PVDF membrane and incubated with biotinylated ... worsens the onset and the rate of progression of atherosclerosis We evaluated the negative effects of Hpt on LCAT activity and cholesterol uptake by hepatocytes by using molar ratios of Hpt with ApoE...
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Báo cáo khoa học: Simultaneous improvement of catalytic activity and thermal stability of tyrosine phenol-lyase by directed evolution ppt
... Relative activity (%) Specific activity (units·mg–1) Temperature (ºC) 10 11 Fig Effect of temperature and pH on stability and catalytic activity of purified TPL proteins (A) Specific activity assayed ... Structural assignments of stabilityand activity- improving mutations in homology model framework (A) and subunit structure (B) of S toebii TPL Red and cyan letters indicate stability and activity mutations, ... remaining activity, mutants AS1–AS7 were selected on the basis of a co-improvement in stability and activity For example, the T1 ⁄ values for AS4 and AS6 were 6.7 and 11.2 °C higher, respectively, and...
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Báo cáo khoa học: The Vps4 C-terminal helix is a critical determinant for assembly and ATPase activity and has elements conserved in other members of the meiotic clade of AAA ATPases pdf
... proteins and recycling late Golgi proteins [13– 16] There are two mammalian isoforms of Vps4, VPS4A and VPS4B, which both function in endocytic trafficking [17] and virus budding [9,18–20] Members of ... by immunoblotting of cell extracts (Fig 1D) The expression level of each of the Vps4p mutant proteins was comparable with that of wild-type Vps4p Thus any loss of function of the mutant proteins ... binding of an ESCRT-III component, Vps20p, to wildtype and mutant Vps4p proteins in the presence and absence of ATP (Fig 5C) Binding of Vps20p to the Vps4p mutant proteins lacking the DEL, TRP and...
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Báo cáo khoa học: Engineering and characterization of human manganese superoxide dismutase mutants with high activity and low product inhibition potx
... backbone amide and carbonyl of Cys140 form hydrogen bonds with the backbone carbonyl and amide of Trp123, the side chain of which forms one wall of the active site cavity and the replacement of which ... reassembly of DNase Idigested fragments, whereas an equal number of units of both Taq and Pfu DNA polymerases were used for the amplification of the reassembled product Preparation of enzymes ... the uncatalyzed dismutation of superoxide [32] A least-square fit of Eqn (1) to the data of Fig yields the constants given in the legend of Fig The values of kcat ⁄ Km and kcat for catalysis by...
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Báo cáo khoa học: Alpha-fetoprotein antagonizes X-linked inhibitor of apoptosis protein anticaspase activity and disrupts XIAP–caspase interaction ppt
... field and a combination of Simplex [65] and Powell algorithms [66] Coordinates of the NMR structures of the BIR2 [49] and BIR3 [50] domains were achieved from the Protein Data Bank files: 1C9Q and ... (500 lg of total protein in 100 lL of buffer C) and activated by addition of lm cytochrome c and mm dATP for 30 at 30 °C in the presence of AFP (6 lg) The reaction mixtures were cooled and incubated ... Addition of AFP in the cytosolic extract induced significant enhancement of the DEVD-ase activity The same caspase activity was detected upon the simultaneous addition of an inhibitory amount of exogenous...
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Báo cáo khoa học: Amino acids at the N- and C-termini of human glutamate carboxypeptidase II are required for enzymatic activity and proper folding pptx
... chromatography and chromatofocusing, as described previously [21] Results Expression and secretion of truncated variants of GCPII To analyze the contribution of individual domains of human GCPII ... domain (domain E) and the D domain of rat GCPII The contribution of domains C and F to the GCPII hydrolytic activity/ inhibitor binding remains to be established The 3D structure of GCPII has not ... NaCl and 20 lL of the GCPII sample, were preincubated for 15 at 37 °C in a final volume of 225 lL A 25 lL mixture of 950 nM ÔcoldÕ NAAG (Sigma) and 50 nM H-labelled NAAG (51.9 CiÆmmol)1; New England...
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Báo cáo khoa học: Sequences downstream of the transcription initiation site are important for proper initiation and regulation of mouse ribonucleotide reductase R2 gene transcription ppt
... reductase is composed of two subunits, proteins R1 and R2 Both subunits are required for enzyme activity and it could be reasonable to assume that the expression of the R1 and R2 mRNAs should be ... numbers represent the intensity of each band normalized to the band in lane The lower numbers represent the intensity of the bands in lanes and normalized to the band in lane In order to map the position ... with high concentrations (50 lM) of polyamide (Fig 1C) Importance of the R2 TATA-box and TBP binding to the R2 promoter We next studied the importance of TBP and the R2 TATA-box in an in vitro...
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Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam xác định các nguyên tắc biên soạn khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản khảo sát chương trình đào tạo gắn với các giáo trình cụ thể tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra đối với đối tượng giảng viên và đối tượng quản lí điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu mở máy động cơ lồng sóc mở máy động cơ rôto dây quấn các đặc tính của động cơ điện không đồng bộ đặc tuyến hiệu suất h fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến tốc độ rôto n fi p2 đặc tuyến dòng điện stato i1 fi p2 sự cần thiết phải đầu tư xây dựng nhà máy chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008 chỉ tiêu chất lượng 9 tr 25