immunohistochemical analysis of human mesenchymal stem cells differentiating into chondrogenic osteogenic and adipogenic lineages
Báo cáo y học: "Chordin knockdown enhances the osteogenic differentiation of human mesenchymal stem cells" pdf
... of BMP-2 and chordinstem cells during osteogenic differentiation of human mesenchymal stem cells (a) Temporal progression of bone morphogenetic protein (BMP)-2 and chordin expression during osteogenic ... Lecanda F, Avioli LV, Cheng SL: Regulation of bone matrix protein expression and induction of differentiation of human osteoblasts and human bone marrow stromal cells by bone morphogenetic protein-2 ... designed candidate siRNAs Subsequently, the cells were transfected with chordin-specific siRNA and were cultured for days (for mRNA analysis) , 10 days (for mRNA analysis and ALP assay), and 21 days...
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Báo cáo y học: "Hypertrophy is induced during the in vitro chondrogenic differentiation of human mesenchymal stem cells by bone morphogenetic protein-2 and bone morphogenetic protein-4 gene transfer" pps
... shown to induce chondrogenic differentiation of mesenchymal progenitor cells and to up regulate the levels of type II collagen and aggrecan in chondrocytes and chondroprogenitor cells [1,7-11] ... chondrogenesis of human mesenchymal stem cells Arthritis Rheum 2008, 58:1377-1388 36 Miljkovic ND, Cooper GM, Marra KG: Chondrogenesis, bone morphogenetic protein-4 and mesenchymal stem cells Osteoarthritis ... other BMPs, BMP-2 and BMP-7 are known to induce differentiation of mesenchymal progenitor cells and preosteoblasts into mature osteoblasts, and to enhance the differentiated function of osteoblasts,...
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Fibronectin and laminin promote differentiation of human mesenchymal stem cells into insulin producing cells through activating Akt and ERK pptx
... conception and design, collection and assembly of data, data analysis and interpretation, and manuscript writing, LLC assisted with collection and assembly of data, data analysis and interpretation, ... differentiation of size-sieved stem cells into electrically active neural cells Stem Cells 2002, 20(6):522-529 Tzeng SF, Tsai MJ, Hung SC, Cheng H: Neuronal morphological change of size-sieved stem cells ... differentiation of human mesenchymal stem cells into insulin producing cells J Biomed Mater Res A 2008, 86(4):1097-1105 Chao KC, Chao KF, Fu YS, Liu SH: Islet-like clusters derived from mesenchymal stem cells...
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Engineered poly(l lactic acid) based nanofibers for osteogenic differentiation of human mesenchymal stem cells
... Liao, Seeram Ramakrishna and Casey K Chan Recovery of Osteogenic Ability of Human Mesenchymal Stem Cells during Serial Passage Using Nanofibrous Scaffolds Proceedings of International Conference ... Chan, and Seeram Ramakrishna Mineralized electrospun nanofibrous scaffolds for directing osteogenic differentiation of human mesenchymal stem cells Proceedings of the 241st ACS Meeting and Exposition, ... images of 2D (A and B), the outer surface of 3D (C and D) and the center of 3D (E and F) electrospun PLLA/Col nanofibrous scaffolds cultured with human MSCs in the Ost medium on day 28 Figures C and...
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Applying macromolecular crowding to promote the expansion and adipogenic differentiation of human mesenchymal stem cells in vitro; an effect of matrix reciprocity
... stromal stem cells J Cell Sci Suppl :63-76 123 Beresford JN 1989 ;Osteogenic stem cells and the stromal system of bone and marrow Clin Orthop Relat Res (240):270-280 124 Caplan AI 1991 ;Mesenchymal stem ... osteogenic differentiation of human mesenchymal stem cells on laminin-5 J Cell Biochem (2):499-514 102 Lozito TP, Kuo CK, Taboas JM, et al 2009 ;Human mesenchymal stem cells express vascular cell ... 1991;Expression of modified bFGF cDNAs in mammalian cells Ann N Y Acad Sci :149-160 120 Kolf CM, Cho E, Tuan RS 2007 ;Mesenchymal stromal cells Biology of adult mesenchymal stem cells: regulation of niche,...
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Báo cáo y học: "Discrepancy between the in vitro and in vivo effects of murine mesenchymal stem cells on T-cell proliferation and collagen-induced arthritis" doc
... and phenotypical analysis The isolated mesenchymal stem cells differentiate into osteogenic and adipogenic lineages To assess the multipotentiality of the cultured mouse MSCs, cells were subjected ... Page of 11 Figure Mesenchymal stem cells (MSCs) inhibit the proliferation of CD4 + T cells in vitro by induction of nitric oxide and prostaglandin E2 (PGE2) (a) CD4+ T cells, in the presence of ... http://arthritis-research.com/content/12/1/R31 Page of 11 Figure Phenotype and differentiation potential of mesenchymal stem cells (MSCs) Bone marrow cells of DBA/1 wild-type and interferon-gamma receptor knockout...
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Báo cáo y học: " GeneChip analysis of human embryonic stem cell differentiation into hemangioblasts: an in silico dissection of mixed phenotypes" docx
... concentrations of BMP4 and VEGF, plus SCF, Tpo and FLT3 ligand (20 ng/ml; R&D Systems), and PTD-HoxB4 (1.5 μg/ml) to expand out BCs and their precursor After 3.5 days, EBs were collected and dissociated ... H, Baker SC, et al.: Genome wide profiling of human embryonic stem cells (hESCs), their derivatives and embryonal carcinoma cells to develop base profiles of U.S Federal government approved hESC ... third level of analysis consists of comparing BCs to tissues they are capable of differentiating into as a way to mask that cell type's 'genetic signature' and reveal signatures of the more minor...
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Vascularised bone tissue engineering endothelial progenitor cells and human mesenchymal stem cells coculture in 3d honeycomb scaffolds and the effect of bi rotational bioreactor and hypoxic microenvironment 1
... 2.8.2.1 Osteogenic Cells 46 2.8.2.2 Mesenchymal Stem Cells 48 2.8.2.3 Mesenchymal Stem Cells in Bone Tissue Engineering .49 2.8.2.4 Proposed Cell Type – Human Fetal Mesenchymal Stem ... J Chan Human endothelial progenitor stem cells enhances osteogenic response of bone marrow derived human fetal mesenchymal stem cells through paracrine signalling mechanisms in vitro and induces ... Zhang, MA Choolani, J Chan Human endothelial progenitor stem cells accelerates and potentiates the osteogenic response of bone marrow derived human fetal mesenchymal stem cells through paracrine...
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Báo cáo y học: "Low temperature tolerance of human embryonic stem cells"
... An efficient and safe xeno-free cryopreservation method for the storage of human embryonic stem cells Stem Cells 2004;22(5):779-89 10 Speicher MR, Ballard SG, Ward DC Karyotyping human chromosomes ... (n=200); and 97.5%, 1.0% and 1.5% respectively for 24h exposure to 25oC (n=200) The proportion of Grade A, B and C colonies were 95.5%, 2.5% and 2.0% respectively for 48h exposure to 4oC (n=200); and ... include quick handling outside the incubator, as well as the use of pre-warmed and pre-equilibrated culture media [15, 16] Indeed, minimizing low temperature exposure of human embryos and oocytes...
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Báo cáo y học: "ene expression analysis of human red blood cells"
... 1-3 - total RNA of human RBCs from different donors; B) typical electropherogram of total RNA of human RBCs Microarray analysis of RNA from human RBCs Recent proteomic studies of RBCs based on ... mechanism of globin m-RNA stability in human RBCs Further experiments are needed to understand the mechanisms and the biological meaning of these findings But gene expression profiling of human erythrocyte ... Microarray and serial analysis of gene expression analyses identify known and novel transcripts overexpressed in hematopoietic stem cells Cancer Res 2004;64:4434-4441 Wiersma PA, Cox GS Synthesis of...
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Atlas of Human Pluripotent Stem Cells doc
... summarizing 12 years of our team’s experience, skill and knowledge in the derivation, culture and expansion of human embryonic stem cells, and more recently, of human induced pluripotent stem cells The ... long-term culture, expansion and manipulation of human pluripotent stem cells The culmination of two events has enabled the production of human embryonic stem cells: the birth of the first IVF test-tube ... reprogramming of adult skin fibroblasts into induced pluripotent stem cells (Takahashi and Yamanaka, 2006) To realize the full potential of embryonic and induced pluripotent stem cells, technologies, and...
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báo cáo hóa học:" MicroRNA and gene expression patterns in the differentiation of human embryonic stem cells" doc
... in adult cells (Figure 6, panel C) The differences in expression of these miRNA expression between adult cells and hES cells and between adult cells and EB were significant (P < 0.05) Of note, ... and miR-520b were much higher in hES cells than in either EB or adult cells (Figure 6, panel A) The difference in the expression of miR-200c, miR-302b, and miR-367 between hES cells and EB, and ... 8-fold greater in hES cells than in EB cells and it was not even detected in adult cells The expression of miR-520b was 26-fold greater in hES cells then in EB cells (P < 0.05) and it was detected...
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Báo cáo sinh học: " Phosphoproteomic analysis of apoptotic hematopoietic stem cells from hemoglobin E/b-thalassemia" pot
... Thailand and two normal donors All patients were children 3-12 years-old and had symptoms of severe anemia and hepatosplenomegaly Hemoglobin analysis showed no expression of the b-hemoglobin band ... The isolated HSCs/CD34+ cells were harvested for phosphoproteomic analysis and cultivation of erythroid cells The cultivation of erythroid cells derived from HSCs/CD34+ cells was performed according ... cytometric analysis of confirmed that the isolated cells highly expressed the cell surface marker CD34 and had low levels of CD45 and were negative for glycophorin A The purity of the isolated CD34+ cells...
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báo cáo hóa học:" Elevated adipogenesis of marrow mesenchymal stem cells during early steroid-associated osteonecrosis development" pptx
... marrow lipid was a consequence of the adipogenesis of marrow mesenchymal stem cells (MSCs) [7] Results of a previous study showed increased number of small size fat cells in the early steroid-associated ... trabecular bone area); fat cells diameter = the total diameter of fat cells in selected field/the number of fat cells in selected field; fat cells area = the area of all fat cells in selected field/(the ... like to thank Professor Huang Lin and Miss Winnie lee from the Department of Orthopedics and Traumatology, the Chinese University of Hong Kong, for their assistance in cells culture and related evaluation...
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o cáo hóa học:" Phosphoproteomic analysis of apoptotic hematopoietic stem cells from hemoglobin E/b-thalassemia" pptx
... Thailand and two normal donors All patients were children 3-12 years-old and had symptoms of severe anemia and hepatosplenomegaly Hemoglobin analysis showed no expression of the b-hemoglobin band ... The isolated HSCs/CD34+ cells were harvested for phosphoproteomic analysis and cultivation of erythroid cells The cultivation of erythroid cells derived from HSCs/CD34+ cells was performed according ... cytometric analysis of confirmed that the isolated cells highly expressed the cell surface marker CD34 and had low levels of CD45 and were negative for glycophorin A The purity of the isolated CD34+ cells...
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Báo cáo y học: "Biology of adult mesenchymal stem cells: regulation of niche, self-renewal and differentiation" potx
... mesenchymal stem cells: characterization, differentiation, and application in cell and gene therapy J Cell Mol Med 2004, 8:301-316 31 Tuan RS, Boland G, Tuli R: Adult mesenchymal stem cells and ... is critical for self-renewal of human multipotent adipose-derived stem cells Stem Cells 2006, 24:2412-2419 Kleber M, Sommer L: Wnt signaling and the regulation of stem cell function Curr Opin ... the stem state of MSCs [14] and other stem cells [15] LIF also activates and represses osteoblast and osteoclast activities [20] The bipotency of LIF suggests that the cellular environment and...
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báo cáo khoa học: "Epigenomics of human embryonic stem cells and induced pluripotent stem cells: insights into pluripotency and implications for disease" potx
... Figure The combination of stem cell models and epigenomics in studies of the role of non-coding mutations in human disease Epigenomic analyses of cells derived through in vitro stem cell differentiation ... embryonic stem cell; hESC, human embryonic stem cell; H3K4me3, trimethylation of lysine of histone H3; H3K27ac, acetylation of lysine 27 of histone H3; H3K27me3, trimethylation of lysine 27 of histone ... embryonic stem cells and differentiation Nat Rev Mol Cell Biol 2006, 7:540-546 51 Park SH, Kook MC, Kim EY, Park S, Lim JH: Ultrastructure of human embryonic stem cells and spontaneous and retinoic...
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Báo cáo y học: "Intradiscal transplantation of synovial mesenchymal stem cells prevents intervertebral disc degeneration through suppression of matrix metalloproteinase-related genes in nucleus pulposus cells in rabbits" potx
... Induction of intervertebral disc-like cells from adult mesenchymal stem cells Stem Cells 2005, 23:403-411 23 Richardson S, Hughes N, Hunt J, Freemont A, Hoyland J: Human mesenchymal stem cell ... MSCs at seven days, and co-culture of rat nucleus pulposus cells with human synovial MSCs at one and seven days (b) Human gene profile of human synovial MSCs in mono-culture and in co-culture with ... Higher chondrogenic potential of fibrous synovium- and adipose synovium-derived cells compared with subcutaneous fatderived cells: distinguishing properties of mesenchymal stem cells in humans...
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Báo cáo y học: "Notochordal conditioned media from tissue increases proteoglycan accumulation and promotes a healthy nucleus pulposus phenotype in human mesenchymal stem cells" pot
... cell-mediated mesenchymal stem cell differentiation Stem Cells 2006, 24:707-716 Le Visage C, Kim SW, Tateno K, Sieber AN, Kostuik JP, Leong KW: Interaction of human mesenchymal stem cells with disc cells: ... Multilineage potential of adult human mesenchymal stem cells Science 1999, 284:143-147 Wuertz K, Godburn K, Neidlinger-Wilke C, Urban J, Iatridis JC: Behavior of mesenchymal stem cells in the chemical ... Heisel C, Hofmann MW, Piecha D, Richter W: Matrix metalloprotease inhibitors suppress initiation and progression of chondrogenic differentiation of mesenchymal stromal cells in vitro Stem Cells Dev...
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Differentiation and derivation of lineage committed chondroprogenitors and chondrogenic cells from human embryonic stem cells for cartilage tissue engineering and regeneration
... plating of hESC-derived chondrogenic cells Fig 17 Chondrogenic differentiation capability of hESC-derived chondrogenic cells Fig 18 Analysis of pluripotency and lineage-restriction of hESC-derived chondrogenic ... III: ISOLATION OF CHONDROGENIC CELLS …………… 133 5.3.1 Human ESC-derived chondrogenic cells ……………………… 133 5.3.1.1 Effects of growth factors and ECM on hESC-derived chondrogenic cells ……………………………………………………………… ... chondrogenic cells ………………………… 83 PHASE III: ISOLATION OF CHONDROGENIC CELLS …………… 86 4.3.1 4.3.2 Expansion of hESC-derived chondrogenic cells …………………88 4.3.3 Differentiation capability of hESC-derived chondrogenic...
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Tìm thêm: khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản khảo sát chương trình đào tạo gắn với các giáo trình cụ thể xác định thời lượng học về mặt lí thuyết và thực tế tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra đối với đối tượng giảng viên và đối tượng quản lí khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu nội dung cụ thể cho từng kĩ năng ở từng cấp độ xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ các đặc tính của động cơ điện không đồng bộ đặc tuyến hiệu suất h fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến tốc độ rôto n fi p2 đặc tuyến dòng điện stato i1 fi p2 động cơ điện không đồng bộ một pha phần 3 giới thiệu nguyên liệu từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng 9 tr 25