identification repetitive element sequence based rep pcr
PERIPHERAL BLOOD BASED C-PCR ASSAY FOR DIAGNOSING EXTRA-PULMONARY TUBERCULOSIS pdf
... tuberculous (89) PCR Status PCR positive PCR negative (%) (%) 04 (100) 00 (0.00) 19 (55.88) 15 (44.12) 39 (43.82) 50 (56.18) Fig 3—Representative agarose gel electrophoresis of PCR products using ... and thawing protocol10 for PCR analysis PCR analysis PCR amplification was performed on isolated DNA samples using specific primers for MPB 64 gene of M tuberculosis The sequence of the primers ... blood based PCR test to detect EPTB cases can replace the need for more invasive diagnostic approaches Interestingly, ours is the first investigation where a single copy target (MPB 64 gene) based...
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Nghiên cứu tính đa dạng của allele hla - DQA1 bằng kỹ thuật polymerase chain reaction sequence specific primers (PCR - SSP) ở dân tộc kinh miền trung - Việt Nam docx
... phản ứng khuyếch đại PCR Kỹ thuật PCR - SSP: Dựa vào phơng pháp O Olerup để tiến hành, cụ thể là: ống tiến hành PCR chứa 10àl gồm có: 75ng tiêu DNA, dung dịch đệm x PCR (50mmol KCl; 1.5mmol MgCl2; ... hành PCR dùng đôi mồi đối chiếu Ctr1 (5 TAT CAT GCC TCT TTG CAC CAT TC3, 23 mer, TM 660C) Ctrl (AAT GCA CTG AAC TCC CAC ATT CC3, 23 mer, Tm 700C) để kiểm soát đối chiếu phản ứng khuyếch đại PCR ... đặc hiệu để khuyếch đại allele DQA1 TT Tên gọi allele Đôi Primers đặc hiệu Sản phẩm PCR Các allele thu hoạch sau PCR DQA1 * 0101/4 A * 5'01 + A * 3'01 DQA1 *0101, DQA1*0104 DQA1 * 0102/4 A * 5'...
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Báo cáo sinh học: "HuMiTar: A sequence-based method for prediction of human microRNA targets" ppt
... approach, in which instead of filtering results of existing sequence- based methods (as done by the ML methods), we develop a novel sequence- based design that Page of 12 (page number not for citation ... http://www.almob.org/content/3/1/16 Based on the above, our experimental section includes three representative miR target prediction methods, TargetScanS, PicTar, and Diana-MicroT The first two were selected based on their ... case of the proposed and the abovementioned sequence- based prediction methods At the same time, we note that the ML methods establish the prediction model based on information concerning both the...
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Báo cáo y học: "A third-generation microsatellite-based linkage map of the honey bee, Apis mellifera, and its comparison with the sequence-based physical map" pps
... chromosomes (G+C-content, gene characteristics, nucleotide polymorphism, repeated sequences) has been analyzed elsewhere [12] reports Chromosomes Solignac et al R66.7 reviews Interference parameter ... order directly given by the calculated map When discrepancies with the sequence persisted, the order of markers was modified according to the sequence and the SLDRs that appeared were controlled ... superscaffolds by adding only 2.5 Mb (5.5% increase) to the five smallest elements (chromosomes 12 to 16) [32] This represents an average length for the inter-scaffold gaps of only 21.9 kb in...
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Báo cáo y học: " A sequence-based survey of the complex structural organization of tumor genomes" pdf
... positive PCR = negative PCR (c) 20 3 MCF7_37E22 37E22 34J23 21C19 30J14 XX X Figure PCR validation of breakpoints in MCF7 PCR validation of breakpoints in MCF7 (a) MCF7 clone 69F1 was sequenced ... analysis, we included 29.7 Mb of sequence from 19,831 end sequenced clones from MCF7 and 701 end sequenced clones from a normal human library (K0241) previously reported [7] Volume 9, Issue 3, ... to the reference sequence, excluding those in repetitive regions, segmental duplications, or duplication-rich centromeric and subtelomeric regions The density of mapped end sequences in ESP closely...
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Báo cáo y học: " Sequence-based prediction of protein-protein interactions by means of codon usage" doc
... Rosetta stone approach Figure 1b also compares PIC with a previous report on identification of protein-protein interactions based on CAI coevolution [14], illustrating up to eight times higher ... Leishmania Genetics 2000, 155:1683-1692 Saccharomyces cerevisiae coding sequences [ftp://genomeftp.stanford.edu/pub/yeast /sequence/ genomic _sequence/ orf_dna/ Genome Biology 2008, 9:R87 http://genomebiology.com/2008/9/5/R87 ... absent from PIT-Lcut600 [22]) are represented A black number between two nodes stands for the reference in which the direct interaction of the two connected nodes is reported A red number refers to...
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Báo cáo y học: " Sequence-based feature prediction and annotation of proteins" ppt
... from sequence While there often is a direct relationship between sequence similarity and conservation of protein structure, the same is not true for protein function: transfer of function based ... proteins [10,11] However, even when two protein sequences not appear to have overall sequence similarity, their alignment can contain short conserved sequence motifs, and these patterns of residues ... kinases that phosphorylate them NetPhorest is a new atlas of consensus sequence motifs with a nonredundant collection of 125 sequence- based classifiers for linear motifs in phosphorylation-dependent...
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Báo cáo khoa học: " Identification and prevalence of Ehrlichia chaffeensis infection in Haemaphysalis longicornis ticks from Korea by PCR, sequencing and phylogenetic analysis based on 16S rRNA gene" docx
... second round nested -PCR, pmol of each primer, HE1 and HE3 (Genotech, Korea) and µl of first PCR product as template DNA were included in the PCR mix described for first round PCR The reaction conditions ... from Gyeonggi province (Table 1) The 396 bp PCR product o o o o o o o o o o o Cloning and sequence analysis PCR amplicons were purified using a GFX PCR DNA Purification Kit (Amersham, UK) according ... tick was sequenced and registered with the GenBank under the accession number AY35042 (strain EC-PGHL) The comparison of nucleotide sequence of strain EC-PGHL with the sequences of 20 representative...
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Báo cáo khoa học: "Analysis of Salmonella enterica serotype Enteritidis isolated from human and chickens by repetitive sequence-PCR fingerprinting, antibiotic resistance and plasmid profiles" ppsx
... siditiretnE 22 fo nevelE siserohportcele dna noitaraperp etalpmet fo snoitidnoc lacitnedi rednu demrofrep saw setalosi eseht fo sisylana etacilpud a nehw elbailer dna elbats saw snrettap tnemgarf fo ... nicakima :swollof sa erew detset scitoibitna ehT ]1[ )ASU ,ocfiD( setalp raga notniH -relluM no demrofrep dohtem ksid noisuffid raga na yb tset ytilibitpecsus laiborcimitna rof deneercs erew setalosI ... desu selcyc RCP ,sremirp CIRE eht roF )ynamreG ,artemoiB( relcyc lamreht AND II ONU a htiw demrofrep erew snoitacifilpmA )aeroK ,reenoiB( esaremylop AND fo U dna setahpsohpirt edisoelcunyxoed Mm...
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Báo cáo khoa học: " Identification of PCR-based markers linked to wood splitting in Eucalyptus grandis" pdf
... markers into SCAR (sequence characterized amplified regions) markers The SCARs were derived by cloning and sequencing the ends of the amplified products of the RAPD marker The sequence was then ... that result in the reproducible amplification of single loci when high annealing temperatures are used The AFLP technique uses genomic DNA digested with restriction enzymes as a PCR template In ... and the individuals were selected by means of forward selection where individuals are evaluated based on their own performance A total of 21 high splitting and 31 low splitting trees were selected...
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báo cáo khoa học: " Isolation, identification and expression analysis of salt-induced genes in Suaeda maritima, a natural halophyte, using PCR-based suppression subtractive hybridization" doc
... was hybridized with the probe prepared from the secondary PCR product of the forward SSH cDNA library and the other with the probe prepared from the secondary PCR product of the reverse SSH cDNA ... preparation was checked spectrophotometrically using NanoPhotometer (Implen, GmbH) The qRT -PCR reaction was conducted using QuantiFast SYBR Green RT -PCR kit (Qiagen, USA) and Opticon-2 qRT -PCR ... Expression study by Real-time RT -PCR of a few unigenes varExpression study by Real-time RT -PCR of a few unigenes varying in EST redundancy Real-time PCR (qRTPCR) was conducted for five unigenes...
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Tài liệu Báo cáo khoa học: Purification and sequence identification of anserinase ppt
... first-round PCR product was then used as a template for nested PCR amplification using a set of nested primers (A and C) As a result, the 281-bp PCR product was specifically amplified A full-length cDNA sequence ... Purification and sequence identification of anserinase S Yamada et al Fig Nucleotide sequence and predicted amino-acid sequence of cDNA encoding Nile tilapia anserinase The putative signal sequence is ... completely replaced with distilled water, using a Centricon-10 for N-terminal sequence analysis N-Terminal sequence analysis and BLAST search Edman degradation was performed on an automated protein sequencer...
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Báo cáo khoa học: "Knowledge-based Automatic Topic Identification" doc
... downward from this interesting wavefront resulting in a second, lower, wavefront, and so on By repeating this process until we reach the leaf concepts of the hierarchy, we can get a set of interesting ... choosing the best one Experiment We have implemented a prototype system to test the automatic topic identification algorithm As the concept hierarchy, we used the noun taxonomy from WordNet (Miller ... Beckwith, Christiane Fellbaum, Derek Gross, and Katherine Miller 1990 Five papers on wordnet CSL Report 43, Congnitive Science Labortory, Princeton University, New Haven, July Chris D Paice 9 ...
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Báo cáo khoa học: "A non-contiguous Tree Sequence Alignment-based Model for Statistical Machine Translation" pptx
... translation model based on non-contiguous tree sequence alignment This model extracts the translation rules not only from the contiguous tree sequence pairs but also from the non-contiguous tree sequence ... grammar based on tree (STSG, Eisner, 2003; Zhang et al, 2007) and tree sequence (STSSG, Zhang et al, 2008a) alignment Besides, we modify the traditional parsing based decoding Non-Contiguous Tree sequence ... source tree sequence covering s([j1, j2]) with each target tree sequence covering [i1,i2] as a non-contiguous tree sequence pair 17: Insert them into p[j1, j2] 18: end if 19: end SncTSSG based Translation...
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Báo cáo khoa học: "A Tree Sequence Alignment-based Tree-to-Tree Translation Model" potx
... presented to combine the strengths of phrase -based and syn- Tree Sequence Alignment Model 3.1 Tree Sequence Translation Rule The leaf nodes of a sub-tree in a tree sequence can be either non-terminal ... model to propose a tree sequence alignment -based translation model To the best of our knowledge, this is the first attempt to empirically explore the tree sequence alignment based model in SMT 561 ... tree sequence- based system For significance test, we used Zhang et al’s implementation (Zhang et al, 2004) We set three baseline systems: Moses (Koehn et al., 2007), and SCFG -based and STSG-based...
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Báo cáo khoa học: Deadenylation of interferon-b mRNA is mediated by both the AU-rich element in the 3¢-untranslated region and an instability sequence in the coding region pot
... Ó FEBS 2003 Sequences governing IFN-b mRNA deadenylation (Eur J Biochem 270) 1591 Based on its sequence, IFN-b ARE belongs to class II Moreover, several reports described IFN-b mRNA ... mRNA, replacement of both elements by control sequences greatly enhances mRNA half-life [13] It should be mentioned however, that these observations were made in heterologous cell systems using reporter ... previously In the pIFNHA construct, the IFN-b gene was tagged by PCR using an oligonucleotide containing three repetitions of the sequence corresponding to the human influenza A virus hemaglutinin...
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Báo cáo khoa học: "Forest-based Tree Sequence to String Translation Model" ppt
... the tree sequence- based model has great potential to address the structure divergence issue by using tree sequence- based non-syntactic translation rules Liu et al (2007) propose the tree sequence ... a tree sequence to string translation model Zhang et al (2008a) propose a tree sequence- based tree to tree translation model and Zhang et al (2008b) demonstrate that the tree sequence- based modelling ... forest -based tree sequence to string translation model Forest -based tree sequence to string model In this section, we first explain what a packed forest is and then define the concept of the tree sequence...
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Báo cáo khoa học: A structured RNA in hepatitis B virus post-transcriptional regulatory element represses alternative splicing in a sequence-independent and position-dependent manner pot
... intron can repress the alternative splicing of SMN1 exon We constructed an ISS reporter in which exon and the adjacent intron sequences from the SMN1 gene were cloned into the EGFP coding sequence ... single-stranded CU-rich sequences to mediate splicing repression [34,37] The accessibility of PTB binding sites would provide this repressor protein with an opportunity to repress splicing However, ... to intronic regions has been shown to repress splicing [45] Sequence- independent enhancer activities have been reported for a pair of complementary intronic elements between exons IIIb and IIIc,...
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Báo cáo khoa học: "Dictionary Definitions based Homograph Identification using a Generative Hierarchical Model" docx
... kernel based NB classifier; however, unlike simple NB it is capable of modeling non-Gaussian distributions Note that in spite of this advantage the kernel based NB is outperformed by the MVN based ... test set and thus we report NB results only A four-fold cross-validation was employed for the all the experiments on the tuning set The results are summarized in Table The reported precision, ... the conditional An additional experiment using two standard classification algorithms – Kernel Based Naïve Bayes (NB) and Support Vector Machines (SVM) was performed We refer to this as the baseline...
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Báo cáo khoa học: "Corpus-Based Identification of Non-Anaphoric N o u n Phrases" potx
... ambiguous, but "the U.S president" is not Restrictive postmodification is often represented by restrictive relative clauses, prepositional phrases, and appositives For example, "the president of the ... test set as referential or existential using the algorithm in Figure Figure graphically represents the main elements of the algorithm Note t h a t we applied vaccines to the S1 and E H P lists, ... say that a definite NP is existential, we say this because it completely specifies a cognitive representation of the entity in the reader's mind That is, suppose "the F.B.I." appears in both...
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- kỹ thuật rep pcr
- performing log sequence based recovery
- 14 determine the visibility of elements in a sequence based layout
- k thut rep pcr
- use of gel based rt pcr for studying induced and tissue enriched gene expression
- identification of causal sequence variants of disease in the next generation sequencing era
- fi c priming and sequence based typing
- high resolution mica genotyping by sequence based typing sbt
- identification of multivariate outliers based on distance computations
- application of repetitive element sequence based rep pcr and denaturing gradient gel electrophoresis for the identification of lactic acid bacteria in probiotic products
- sequence based tracking under unreliable sensing
- nucleic acid sequence based amplification
- development of a model based upon pcr
- kỹ thuật nasba nucleic acid sequence based amplification
- apos s circular sequence based prob method
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