use of gel based rt pcr for studying induced and tissue enriched gene expression
Báo cáo hóa học: " Development and evaluation of one step single tube multiplex RT-PCR for rapid detection and typing of dengue viruses" pdf
... successful isolation of Dengue type 2, and viruses (Table 1) RNA was extracted from culture supernatant and used as template in RT- PCR and nested PCR The RT- PCR and nested PCR revealed desired ... standardization of all RT- PCR experiments, virus isolation, and evaluation of the M -RT- PCR assay PKD carried out the nested PCR and In vitro transcription and sequencing SSR carried out the RT- PCR, ... slow and difficult to perform The molecular methods based on PCR technique offer a suitable alternative to conventional isolation technique The RT- PCR targeting the conserved regions of C-prM gene...
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báo cáo hóa học:" A promising method for identifying cross-cultural differences in patient perspective: the use of Internet-based focus groups for content validation of new Patient Reported Outcome assessments" potx
... more formal involvement as members of the PRO design team Greater use of moderators in PRO content development activities is a good use of expertise, due to their deep emersion in, and understanding ... last decade The transformation of text -based bulletin-board services into multimedia 'blogs' and virtual community networks have lead to a proliferation of both formal and informal discussion groups ... participation; 2) the ease and speed of participant engagement, facilitation and surveying; and 3) the automated management of resulting transcripts and survey data [4] Demonstration that virtual...
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báo cáo khoa học: " A quasi-experimental test of an intervention to increase the use of thiazide-based treatment regimens for people with hypertension" docx
... outcomes; and oversee computer programming algorithms to use with the medical center's data warehouse for the prevalence of hypertension, use of thiazides and other anti-hypertensives, and blood ... diabetes [11] Because of the high prevalence of hypertension in VA beneficiaries and the relatively low rates of thiazide use [12,13], full-scale implementation of thiazide- based antihypertensive regimens ... Proportion of hypertensive patients on a thiazide -based regimen, General Medicine vs PrimeCare, July 2002 through March Proportion of hypertensive patients on a thiazide -based regimen, General...
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báo cáo khoa học: "Effects of an evidence service on community-based AIDS service organizations’ use of research evidence: A protocol for a randomized controlled trial" pptx
... capacity to find and use research evidence) or ‘exchange’ efforts, which focus on the producers and users of researchers building partnerships and working collaboratively in the production and interpretation ... least helpful and why, and recommendations for next steps In addition, we will ask participants about any recommendations for how to improve upon our efforts to support the use of research evidence ... of what we learned from the interviews and invite comment on it) Finally, we will use the document analysis of the comments left in the user forum to help further our understanding of how participants...
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Báo cáo y học: " The development of a rapid SYBR one step real-time RT-PCR for detection of porcine reproductive and respiratory syndrome virus" potx
... at -70°C for further use The primers used for real-time RT- PCR amplification of PRRSV were designed using sequence data from the PRRSV N protein gene The partial sequence of the N gene of PRRSV ... at -70°C for further use Table 3: Diagnostic field samples tested positive by real-time qRT -PCR or conventional RT- PCR Tissue samples Number Conventional RT- PCR positive Real-time qRT -PCR positive ... real-time RT- PCR Plot of the amplification of a 10-fold serial dilution of ch-1a RNA to calculate the detection limit and sensitivity of real-time RT- PCR by analyzing the fluorescence curve of the...
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Báo cáo khoa học: " A real-time RT-PCR for detection of clade 1 and 2 H5N1 Influenza A virus using Locked Nucleic Acid (LNA) TaqMan probes" pps
... Page of conventional RT- PCR, rRT -PCR has a smaller risk of cross-contamination, higher sensitivity and specificity, and shorter per sample laboratory turnaround time Several rRT -PCR assays for ... evidence of co-circulation of clade and clade H5N1 viruses in South East Asia has highlighted the need for RT- PCR assays that allow detection of both genetic clades We developed a single step rRT -PCR ... plasmid, and less than 0.5 PFU of H5N1 viruses per reaction, and specific for the detection of influenza A of subtype H5 The HA gene of clades 1, 2.1, and 2.3 was amplified from both virus isolates and...
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The use of theme-based short readings to improve vocabulary for freshmen at Hanoi Medical College = sử dụng các bài đọc ngắn theo chủ đề nâng cao vốn từ vựng ch
... way of reinforcing, confirming and deepening knowledge of vocabulary and expressions hitherto only imperfectly known, and of developing an implicit understanding of when and how words are used, ... sentences are used for listening and speaking, and the words which we recognize and use for reading and writing can be identified as written vocabulary The words that we use to write and speak are ... to pleasure, information, and general understanding and are determined by the nature of the material and the interest of the students Students are encouraged to read for a variety of real-world...
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A STUDY ON THE USE OF CLASSROOM DISCIPLINE AS MOTIVATION FOR SECOND LANGUAGE ACQUISITION IN THE CONTEXT OF ENGLISH LANGUAGE TEAC
... as an important aspect of SLA, because language is one of the most observable expressions of culture and because in second (as opposed to foreign) language settings the acquisition of a new language ... result of conscious study of the formal properties of the language In performance, “acquired” knowledge serves as the major source for initiating both the comprehension and production of utterances ... the teachers and students of Hanoi School for Foreign Language Gifted Students in taking part in the survey and providing useful feedback, which are fundamental in the completion of this study...
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Tài liệu Notes on the use of hand-held test devices for installation of the KRONE Cat.6 KM8 products doc
... on the use of hand-held test devices for installation of the KRONE Cat.6 KM8 products December 2001 Page of 23 Cat 6/5e Channel Adapter DSP-LIA012S: § Contains two interface adapters for use with ... on the use of hand-held test devices for installation of the KRONE Cat.6 KM8 products December 2001 Page of 23 Cat 6/5e Channel Adapter N2604A-100: § Includes two interface adapters for use with ... of the cable length by the instrument Notes on the use of hand-held test devices for installation of the KRONE Cat.6 KM8 products December 2001 Page of 23 IDEAL LT8600LT The IDEAL LT8600 performs...
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USE OF MINIMAL LEXICAL CONCEPTUAL STRUCTURES FOR SINGLE-DOCUMENT SUMMARIZATION doc
... Automatic Generation of Informative Cross-Lingual Headlines for Text and Speech Thesis Proposal, University of Maryland, 2003 5.3 OTHER PRODUCTS Trimmer: Trimmer generates a headline for a news ... Nizar Habash, University of Maryland, habash@umiacs.umd.edu Christof Monz, University of Maryland, christof@umiacs.umd.edu STUDENTS: Stacy President, University of Maryland, stacypre@umiacs.umd.edu ... the documents by a factor of 4 OPPORTUNITIES FOR TRAINING AND DEVELOPMENT (AT ALL GRADE LEVELS) The automatically generated headlines allow users to assess the relevance of a document in a time...
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study of wo3-based sensing materials for nh3 and no detection
... sensitivity and responding properties of WO decayed with loading of alkali metals The responding and recovery properties were obviously improved by loading with alkali earth metals The materials of WO ... surface of the WO 3 -based sensing element Two kinds of NO reduction catalysts of TiO q 10 wt.% CuO q wt.% SiO and TiO q wt.% V2 O5 q wt.% WO q wt.% SiO were used for the coating layers, and measured ... S s 5.40 for 30-ppm NH and S s 4.00 for 40-ppm NO; WO q wt.% Mg, Zn, Mo and Re: S s 7.65, 5.06, 10.1, 9.46, respectively, for 30 ppm NH , and S s 5.87, 8.33, 5.00, 6.25, respectively, for 40 ppm...
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THE ATTRACTIONS OF RISK-BASED REGULATION: ACCOUNTING FOR THE EMERGENCE OF RISK IDEAS IN REGULATION docx
... adoption of risk based tools and an uneven use of the language and rhetoric of risk The elements of risk -based approaches are various At a minimum they entail the use of technical risk -based tools, ... investigation, the design of premises and plant, and provision for audit The importance of risk based tools and perspectives in the area of occupational health and safety area is further evidenced by ... objectivity and reliability of technical risk assessment and economic tools may be contested so too is the status of scientific and other forms of expert knowledge Generally the public and media...
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Báo cáo khoa học: " Development and Use of a Gold-Standard DataSet for Subjectivity Classifications" pdf
... potentially important for many text processing applications, such as information extraction and information retrieval, where the evidential status of information is important In generation and machine ... held-out portion of the training data 10-fold cross validation is performed The data is partitioned randomly into 10 different SFor the analysis in Table 3, certainty ratings and 1, and and are ... i.e., the number of values of L, and produces estimates of the 249 The judges are given lists of sentences for which their tags differ from the biascorrected tags Judges M, D, and J participate in...
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báo cáo hóa học: "Use of medications by people with chronic fatigue syndrome and healthy persons: a population-based study of fatiguing illness in Georgia" pptx
... of the Centers for Disease Control and Prevention and adhered to the human research guidelines of the U.S Department of Health and Human Services All participants were volunteers who gave informed ... range 1–18) Overall, 85.8% of the entire sample (430 of 501) used at least one drug: 92.9% of CFS, 83.7% of ISF and 83.9% of the Well group The mean number of drugs used per person in the CFS ... reported reason (22.2%) in the CFS group The profile of NSAID use differed between persons with CFS and Well controls Among persons taking NSAIDs, 49.1% of the users in the CFS group used ibuprofen...
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Báo cáo hóa học: " The use of RNA-dependent RNA polymerase for the taxonomic assignment of Picorna-like viruses (order Picornavirales) infecting Apis mellifera L. populations" pptx
... they used quantitative PCR to investigate DWV prevalence, with the forward primer containing no mismatches for KV and for VDV, the reverse having no mismatches for KV and mismatches for VDV, and ... ascertain whether certain viruses are present in any given sample and thus will affect the prediction of cause and effect The data presented here provides further foundations for understanding ... helicase and structural genes of this virus [28] No clear geographical pattern of distribution was identified based on the phylogenetic analysis of these genes either, suggesting that other genes...
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báo cáo hóa học:" Use of medications by people with chronic fatigue syndrome and healthy persons: a population-based study of fatiguing illness in Georgia" doc
... of the Centers for Disease Control and Prevention and adhered to the human research guidelines of the U.S Department of Health and Human Services All participants were volunteers who gave informed ... range 1–18) Overall, 85.8% of the entire sample (430 of 501) used at least one drug: 92.9% of CFS, 83.7% of ISF and 83.9% of the Well group The mean number of drugs used per person in the CFS ... reported reason (22.2%) in the CFS group The profile of NSAID use differed between persons with CFS and Well controls Among persons taking NSAIDs, 49.1% of the users in the CFS group used ibuprofen...
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Báo cáo hóa học: " Research Article Minimum Probability of Error-Based Equalization Algorithms for Fading Channels" docx
... optimized empirically In the case of Figures and 10, we set γ = 0.1 for TGS and TGSI, γ = 0.002 for BBEAd, γ = 0.01 for LMS, and γ = 0.2 for AMBER, respectively In the case of AMBER, we set the learning ... N0 , PROOF OF THEOREM The proof of inequalities (19a) and (19b) follows from the application of the Cauchy-Schwarz inequality to PD(w) in the bounds given by the right-hand side of (16a) and (16b), ... SNR performance of the different methods in the case of channel h(3) , equalizer coefficients and D = Figure 1: PE versus SNR performance of the different methods in the case of channel h(1) and equalizer...
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Báo cáo hóa học: " Research Article On the Utility of Syllable-Based Acoustic Models for Pronunciation Variation Modelling" pptx
... number of training tokens for further training were also trained further in the mixed-model recogniser The minimum frequency for further training was 20 in the case of TIMIT and 40 in the case of ... recognition performance (using a significance test for a binomial random variable), as compared with the performance of the triphone recognisers However, the performance of the syllable-model and of the ... the articulation rates of TIMIT and CGN differed We defined the articulation rate as the number of canonical phones per second of speech The rates were 12.8 phones/s for TIMIT and 13.1 phones/s for...
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Tìm thêm: xác định các mục tiêu của chương trình khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản xác định thời lượng học về mặt lí thuyết và thực tế tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra đối với đối tượng giảng viên và đối tượng quản lí điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam nội dung cụ thể cho từng kĩ năng ở từng cấp độ xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct mở máy động cơ rôto dây quấn các đặc tính của động cơ điện không đồng bộ đặc tuyến hiệu suất h fi p2 đặc tuyến mômen quay m fi p2 sự cần thiết phải đầu tư xây dựng nhà máy thông tin liên lạc và các dịch vụ phần 3 giới thiệu nguyên liệu từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008 chỉ tiêu chất lượng 9 tr 25