i structure and function
Tài liệu Báo cáo khoa học: Structure and function of active chromatin and DNase I hypersensitive sites pdf
... transcription factors not have an intrinsic ability to bind efficiently to recognition sequences within chromatin, and it is also evident that most consensus sequences within the genome are not utilized ... activation by stimulation of calcium and kinase signalling pathways, nucleosomes throughout this kb region adopt a highly disorganized structure with nucleosomes redistributed to random positions ... intrinsic resistance to folding into a rigid compacted structure Similar nucleosome mobilization within active loci has been observed in many model systems (which I have summarized previously...
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Tài liệu Báo cáo khoa học: Interaction between very-KIND Ras guanine exchange factor and microtubule-associated protein 2, and its role in dendrite growth – structure and function of the second kinase noncatalytic C-lobe domain docx
... binding, which is absent in the KINDs from other KIND-containing proteins To examine the dendrite localization signal domains in v-KIND, we first investigated the subcellular localization of eight ... stain) Fig Residues 456–487 within the v-KIND KIND2 domain contain the MAP2-binding core site (A) Division of the KIND2 domain (456–620) into five subregions: KIND2-1 (amino acids 456–487), KIND2-2 ... sequence critical for the specific v-KIND KIND2 binding resides within residues 702–744 of MAP2 Residues 456–487 in the KIND2 domain of v-KIND contain the core MAP2 binding module To identify the...
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Tài liệu Body Size: The Structure and Function of Aquatic Ecosystems pptx
... elements: (i) direct interception, (ii) inertial impaction, (iii) gravitational deposition, (iv) diffusional deposition and (v) electrostatic attraction Simple sieving (where particles are caught ... (i) positive net energy gain is possible, and (ii) costs and gains increase at a similar rate with increasing size of the capture apparatus, suspension feeding will be viable given feeding structure( s) ... this critical size will be retained Thus, if sieving is utilized by a particular species, a bimodal prey-size distribution is predicted (Silvester, 1983; Brown et al., 2005) and maximum prey size...
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Tài liệu Báo cáo khoa học: Structure and function of plant aspartic proteinases pptx
... gene involved in disease resistance [24] In general, plant APs share high amino acid sequence similarity in their N- and C-terminal domains (over 60% identity), and about 45% identity with cathepsin ... variability in the intron sizes, the typical and nucellinlike Arabidopsis APs exhibit conservation of exon/intron organization when compared to the coding region of AP genes from Brassica, rice ... Wieczorek, M & Wilimowska-Pelc, A (1985) Purification and characterization of aspartic proteinases from Cucumis sativus and Cucurbita maxima seeds In Aspartic Proteinases and their Inhibitors (Kostka,...
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Tài liệu Báo cáo khoa học: a-Conotoxins as tools for the elucidation of structure and function of neuronal nicotinic acetylcholine receptor subtypes doc
... a-conotoxin genes from C imperalis genomic DNA and cDNA [35] Despite having 75% amino acid identity and showing similar activity in bioassays and on oocyte-expressed a7 receptors, ImI and ImII appear ... properties of the channel In situ hybridization and immunohistochemistry data show overlapping distributions for a variety of subunits, and electrophysiological and other functional studies in native ... terminals This model is based on the results from a variety of binding studies using MII and the radioligand 12 5I- labelled MII on knockout mice [74,92] and immunoprecipitation studies using rat...
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Tài liệu Báo cáo khoa học: Cold survival in freeze-intolerant insects The structure and function of b-helical antifreeze proteins pdf
... putative ice-binding face of each model is oriented towards the viewer residues) were found on two faces of the protein, which, in combination with its superior ice-recrystallization inhibition, ... Sykes, unpublished observation), and given the irreversibility of the gel formation, the change in structure is unlikely to provide effective protection against in vivo ice growth The structure ... that it is not only the binding of AFP to two ice faces that result in a higher activity, but that the activity increases with an increase in the number of residues that bind ice (and hence increases...
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Báo cáo khoa học: NMR solution structure and function of the C-terminal domain of eukaryotic class 1 polypeptide chain release factor pdf
... overproduced in Escherichia coli BL21(DE3), in M9 minimal medium, and isolated using Ni2+–nitrilotriacetic acid resin (Qiagen) The protein was further purified by cation exchange chromatography, using HiTrap ... produced in His6-tags E coli BL21(DE3), and purified as described previously [6,13,35] Purification of initiation and elongation factors, ribosomal subunits, and aminoacylation of tRNA Pretermination ... [35S]Met-containing tetrapeptide, which indicated the efficiency of peptidyl-tRNA hydrolysis, was determined by scintillation counting of the supernatants using an Intertechnique SL-30 liquid scintillation...
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Báo cáo khoa học: Evolutionary changes to transthyretin: structure and function of a transthyretin-like ancestral protein doc
... transthyretin-like protein A ii B i Fig The active site of TLP (A) Comparison of the ligand-binding cleft at the dimer– dimer interface in (i) human transthyretin with (ii) Salmonella dublin TLP Residues ... oxidase activity in hominoids and its evolutionary implications Mol Biol Evol 19, 640–653 Kaur H & Halliwell B (1990) Action of biologically relevant oxidising species upon uric acid Identification ... protein (ii) The equivalent region in TLP is shallow and positively charged (Adapted from [30]) i ii C i ii Table Site-directed mutagenesis of conserved residues in TLP Transthyretin residue Equivalent...
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Báo cáo khoa học: Structure and function of KH domains docx
... elements in individual eukaryotic type I KH domains different from that in prokaryotic type II KH domains, but the relative orientation of tandem type I versus type II KH domains is also quite different ... domains (Fig 3) This relationship holds true in all families and between species, from those within which the like-pairs of domains have very high identity [over 95% in the Nova and poly(C)-binding ... specific interactions stabilizing the complex Nucleic acid base-to-protein aromatic side-chain stacking interactions, which are prevalent in other types of single-stranded nucleic acid binding motifs...
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Báo cáo khoa học: Structure and function of N-acetylglucosamine kinase Identification of two active site cysteines pptx
... subdomains are highlighted in different gray scales Secondary structures in common with glucokinase are indicated with: I, a-helix; II, b-sheet; III, a-helix (B) Top view of the threedimensional structure ... the activity of glucokinase [22] The distribution of a-helices and b-sheets in GlcNAc kinase and glucokinase showed a very similar pattern Highest similarities were found within the ATP-binding ... cysteine Fig Relative specific activities of GlcNAc kinase and cysteine mutants GlcNAc kinase activities were determined in the cytosolic supernatants of E coli BL21 cells as described in Experimental...
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Day one nucleic acid structure and function
... profile could be associated with an individual These DNA profiles have revolutionized criminal investigations and have become powerful tools in the identification of individuals in criminal and ... in boiling 0.1 M hydrochloric acid in 30 rA, rG hydrolyzed in boiling M hydrochloric acid in 60 rC, rU hydrolyzed in boiling 12 M perchloric acid in 60 DNA is quite stable under basic conditions ... Adenine and Cytosine are methylated more often than Guanine and Thymine Methylation is confined to specific regions of DNA and aid in biological processes E coli DNA is methylated to distinguish...
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Cell Structure and Function Functi
... – 4.9) Series of organelles responsible for: Modifying protein chains into their final form Synthesizing of lipids Packaging of fully modified proteins and lipids into vesicles for export ... proteins are modified in RER and lipids are made in SER vesicles containing the proteins and lipids bud off from the ER Endomembrane System Putting it all together ER vesicles merge with Golgi ... proteins and lipids enter Golgi each is fully modified as it passes through layers of Golgi modified products are tagged, sorted and bud off in Golgi vesicles … Endomembrane System Putting...
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CELLS Structure and Function Structure and Function
... Collects, modifies, packages and distributes proteins and lipids from ER Functions of Eukaryotic Cell Features Structure Lysosome Function( s) Contains digestive enzymes to digest food in protists or ... cell? B Which organelle contains digestive enzymes? C Which site acts as a protein packaging and distribution center? Thought Questions What are similarities for a eukaryotic and prokaryotic cells? ... Contractile vacuole (protists) maintains water balance Food vacuole (protists) fuses with lysosome for digestion Functions of Eukaryotic Cell Features Structure Cilia and Flagella Function( s)...
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Báo cáo khóa học: S-(2,3-Dichlorotriazinyl)glutathione A new affinity label for probing the structure and function of glutathione transferases potx
... nm, and was negative in the 5,5-dithio-bis-(2-nitrobenzoic) acid test for free -SH groups and positive in the ninhydrin and 2,4,6-trinitrobenzenesulfonic acid test for primary amine The chloride ... triazine-based affinity labels are their high stability in neutral buffer solutions and the presence of electron-rich nitrogen sites on the triazine ring, which increase the capability of forming ... high GST activity This probably affords an enzyme conformation similar to that adopted during catalysis, thus creating more favourable conditions for ligand binding The kinetics of inactivation...
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Báo cáo khoa học: Multiple effects of DiS-C3(5) on mitochondrial structure and function pot
... of mitochondria treated with DiS-C3(5) in the absence of Pi may give us insight into the mechanisms causing configurational changes in mitochondria Until now, both CsA-sensitive and insensitive ... typical PT inducer Like that of Ca2+, the addition of 50 lM DiS-C3(5) to the mitochondrial suspension caused a massive decrease in its turbidity, reflecting induction of mitochondrial swelling ... to the induction of mitochondrial PT To validate this interpretation, we further characterized the actions of DiS-C3(5) on the mitochondrial structure and function and compared them with those...
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Báo cáo khoa học: Human haptoglobin structure and function – a molecular modelling study pptx
... tetramers and higherorder multimers (Fig 5) Hb binding and chaperone-like activity of HPT Selective proteolysis studies have demonstrated that the Hb-binding site lies in the region surrounding residues ... giving rise to a Modelling haptoglobin structure HPT1 HPT2 Fig Quaternary structure model of dimeric HPT1 and trimeric HPT2 Interchain disulphide bonds are shown in spacefill representation bilobated ... promoting immediate clearance of free Hb, acts as an antioxidant agent [37] An additional activity of HPT is related to its ability to suppress heat-induced and oxidative stress-induced unfolding and...
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Báo cáo khoa học: Quaternary structure and functional properties of Penaeus monodon hemocyanin docx
... residues; + ⁄ –, gain ⁄ loss of positive charges M Beltramini et al 2065 P monodon hemocyanin structure and function M Beltramini et al Table Amino acids involved in the pairwise interactions in ... vannamei (Pen va.), Q26180, CAA57880 The amino acids found in the indicated positions of various Hc are listed For details see the text NS, not significant substitutions; I, isofunctional substitutions; ... dissociate under typical dissociating conditions Among those residues that may be involved in intersubunit interactions within hexamers, only a few are substituted in Hc from P vannamei, possibly...
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Báo cáo khoa học: N1 – IUBMB 50th Anniversary Symposium: Protein Structure and Function potx
... Taddei1, C Parrini1, F Piccioli2, L Messori2, C Canale3, A Gliozzi3 and F Chiti1 ` Dipartimento di Scienze Biochimiche, Universita di Firenze, ` Firenze, Italy, 2Dipartimento di Chimica, Universita ... Alpha, Mu, Pi and Theta classes on the basis of a combination of criteria such as substrate/inhibitor specificity, primary and tertiary structure similarities and immunological identity In this study, ... proteomics I Pucci-Minafra, P Cancemi and S Minafra Laboratorio di Genomica e Proteomica, Dipartimento di Oncologia Sperimentale e Applicazioni Cliniche, Centro di Oncobiologia Sperimentale, Universita`...
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Báo cáo khoa học: F1 – Structure and Function of Motor Proteins pot
... Department Biology, Chongqing University of Medical Sciences, Chongqing City, PR China, 2Key Lab of Biomechanics & Tissue Engineering, Institute of Bioengineering, Chongqing University, Chongqing City, ... by an increase in ribosomal A-site binding, extreme sensitivity to paromomycin, and higher initial rates of protein synthesis activity, all compatible with its error-prone character Unlike in E ... and (viii) In vivo experiments (injection of D-Asp into the whole animal) or in vitro experiments (incubating isolated neurons with D-Asp) induced a significant increase of cAMP F2-023P Experimental...
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Báo cáo khoa học: Structure and function of human a-lactalbumin made lethal to tumor cells (HAMLET)-type complexes pptx
... aromatic residues and oleic acid, indicative of the direct interactions between oleic acid and the aromatic residues [6] In addition, ELOA is characterized by similar thermal stability to equine ... by simple mixing and co-incubation of apo a-lactalbumin or equine lysozyme in solution with oleic acid either under native, mildly denaturing acidic (pH and 4.5) or basic (pH 9) conditions In ... matrix is positively charged under the experimental conditions and oleic acid is bound to the matrix before ELOA formation It is speculated that during interaction with the solid–liquid interface...
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