human salivary gland stem cells isolation propagation and characterization
Báo cáo khoa học: A comparative analysis of the transcriptome and signal pathways in hepatic differentiation of human adipose mesenchymal stem cells doc
... RY, Huang CT, Tsai CC, Shen EY & Chiu WT (2005) Isolation and characterization of neurogenic mesenchymal stem cells in human scalp tissue Stem Cells 23, 1012–1020 In’t Anker PS, Scherjon SA, ... (2004) Isolation of mesenchymal stem cells of fetal or maternal origin from human placenta Stem Cells 22, 1338–1345 Bieback K, Kern S, Kluter H & Eichler H (2004) Critical parameters for the isolation ... into hepatic cells, the findings presented here suggest that MET might be a pivotal factor in determining stem cell transdifferentiation Discussion AT-MSCs may be good candidates as stem cells for...
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Báo cáo hóa học: "Future research and therapeutic applications of human stem cells: general, regulatory, and bioethical aspects" ppt
... embryonic stem cells in acute spinal cord injuries [107,108] Human stem cells, mainly autologous bone marrow cells, autologous and allogeneic mesenchymal cells, and some allogeneic neural cells, ... induced pluripotent stem cells are at least, if not more capable than those from adult (stem) cells Mesenchymal stem cells Although adult stem cells are primarily unipotent cells, under certain ... infarction, and in stroke [31,32] Other types of stem cells Hematopoietic stem cells together with mesenchymal stem cells, the so-called “side population”, and multipotent adult progenitor cells (MAPCs),...
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Báo cáo y học: " Gene expression profiling of human prostate cancer stem cells reveals a pro-inflammatory phenotype and the importance of extracellular matrix interactions" pps
... data analysis and design SL performed the stem cell isolations from benign samples PB performed stem cell isolations from tumors CH performed culturing and isolation of stem cells, and NF-κB experiments ... signature Stem cells Stem cells (c) Committed basal Committed basal Stem Committed cells basal (d) Malignancy signature Tumour Birnie et al R83.3 Gleason included Benign Figure Distinctive stem cell and ... Materials and methods Tissue collection, isolation, and culture of tumor stem cells Human prostate tissue was obtained, with patient consent, from 12 patients undergoing radical prostatectomy and transurethral...
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Human embryonic stem cell derived neural stem cells derivation, differentiation and MicroRNA regulation
... Pluripotent stem cell-derived NSCs Human embryonic stem cells (hESCs), which are pluripotent cells derived from the inner cell mass of blastocyst-stage embryos, offer a renewable and unlimited ... This strand is referred to as the guide strand The complementary strand, known as the passenger strand or miRNA*, is degraded in most cases and present in lower levels than the guide strand (Schwarz ... and SOX2, which are expressed in early neuroectodermal cells and NSCs, provided proof that PAX6 and SOX2 function as key determinants of human neuroectodermal fate that promote neurogenesis and...
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Directed differentiation of human embryonic stem cells into haematopoietic and definitive endodermal lineages
... Mu ° – Degree x CHAPTER 1: GENERAL INTRODUCTION 1.1 Embryonic stem cells The isolation of mouse and human embryonic stem cells (ES cells) heralded a new era in regenerative medicine, raising hopes ... Embryonic origin of human embryonic stem cells and their in vitro characterisation Figure 1.2 Lineage tree of embryo-derived cells and cell lines Figure 1.3 Gastrulation and specification of ... Embryonic stem cells 1.2 Gastrulation– formation of mesoderm and endoderm in the embryo .8 1.3 Regenerative medicine and embryonic stem cells 10 1.3.1 Diabetes– a candidate...
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Báo cáo hóa học: " CdTe quantum dots with daunorubicin induce apoptosis of multidrug-resistant human hepatoma HepG2/ADM cells: in vitro and in vivo evaluation" pptx
... and DNR on HepG2/ADM cells Signal pathway of treatments in HepG2/ADM Cells Figure MTT assay of the growth inhibition rate of HepG2/ ADM cells after various cellular treatments The HepG2/ADM cells ... outside HepG2/ADM cells after cell treatment for h (a) PBS; (b) μM Cdte QDs + × 10-6 mol/L DNR treatment and cells for h; and (c) μM DNR Pulse amplitude, 0.05 V; pulse width, 0.05 s; and pulse period, ... rate and morphological images: (A) Detection of apoptotic and normal cells by acridine orange staining Control cell nuclei, apoptotic nuclei from HepG2/ADM cells ware observed (B) HepG2/ADM cells...
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Báo cáo y học: "B cell-activating factor of the tumor necrosis factor family (BAFF) is expressed under stimulation by interferon in salivary gland epithelial cells in primary Sjögren''''s syndrome" ppt
... analysis of salivary epithelial cells Cells were obtained from minor salivary glands (a–e) and the HSG cell line (f–i) Positive cells staining for cytokeratin (a) and cytokeratin 19 (b) and the absence ... demonstrated previously [44] Figure cells from minor salivary glands Immunocytochemical analysis of BAFF expression in salivary epithelilal cells from minor salivary glands Positive staining for B cell-activating ... expression was observed in cells stimulated by IL-10 or TNF-α Induction of BAFF mRNA by epithelial cells from minor salivary glands glands Results are from seven patients with pSS and from seven controls,...
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Báo cáo y học: " Over-expression of HO-1 on mesenchymal stem cells promotes angiogenesis and improves myocardial function in infarcted myocardium" pdf
... homologous recombination and propagated in 293 cells At stipulated time, the supernatant from 293 cells was collected and purified on cesium chloride (CsCl) gradient centrifugation and stored in 10 mmol/L ... inhibited apoptosis and improved heart function Stem Cells 2007, 25:2118-2127 Wang D, Shen W, Zhang F, Chen M, Chen H, Cao k: Connexin43 promotes survival of mesenchymal stem cells in ischaemic ... Page of After labeling, cells were washed six times in D-Hanks solution to remove unbound DAPI and then the cells were observed using fluorescent microscopy Cell implantation and trafficking of the...
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Báo cáo khoa hoc:" Human spongiosa mesenchymal stem cells fail to generate cardiomyocytes in vitro" doc
... confluence (about 10,000 cells/ cm2), stem cells were trypsinized with stem cell trypsin (CellSystems, St Katharinen, Germany) and re-plated at a density of 1000/cm2 to expand cell culture (cell ... range of immune cells, including N -cells, Bcells, natural killer cells and antigen presenting cells Transplantation of MSCs into the periinfarct area decreases both production and gene expression ... Nkx2.5 and smooth muscle actin (SMA) The slight expression of MYH7B, TnI and Nkx2.5 by all cells and SMA expression by some single cells was revealed Co-culture of spongiosa hMSCs and Cor.AT cells...
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Báo cáo y học: "Regulation of the cd38 promoter in human airway smooth muscle cells by TNF-α and dexamethasone" pot
... from a human erythropoietic cell line (K562 cells) in our laboratory revealed binding sites for NF-κB, AP-1, and GR (summarized in Table 1) To determine whether CD38 expression in human ASM cells ... assay system and luciferase activities were measured with a luminometer (Lumat LB9507; Berthold) Cells were washed twice with phosphate-buffered saline (PBS) with no calcium and magnesium, and covered ... growth-arrested HASM cells at confluence The media were aspirated and washed with ice-cold PBS containing phosphatase inhibitors and the cells were scraped in ml of the same buffer The cells were pelletted...
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Báo cáo y học: "Comparative transcriptome analysis of embryonic and adult stem cells with extended and limited differentiation capacity" pps
... (NMF) and kmeans clustering to analyze their transcriptomes and determine the relationship between MAPCs, MSCs and ESCs, using mouse and rat MAPCs and mouse MSCs and ESCs Results Isolation and characterization ... [1,2] Other adult stem cells include neural stem cells (NSCs) [3], and the mesenchymal stem cells (MSCs) that give rise to osteoblasts, chondrocytes, adipocytes, and skeletal and smooth muscle myocytes ... neural stem cells Science 2000, 287:1433-1438 Prockop D: Marrow stromal cells as stem cells for nonhematopoietic tissues Science 1997, 276:71-74 Smith AG: Embryo-derived stem cells: of mice and...
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Báo cáo y học: " Human herpesvirus-8 in northwestern China: epidemiology and characterization among blood donors" pdf
... Kazak, Mongolian, and others All samples went through a standard screening for HBV, HCV, HIV, and syphilis A questionnaire regarding age, sex, ethnicity, marital status, education and residence was ... sample collection, and statistical analyses performance; she also participated in antibody detection ZZ, TL, QZ and HW also participated in antibody detection XLi and XLu wrote and collected the ... of the study and carried out statistical analyses HW conceived the study, and participated in its design and coordination; he also helped to draft the manuscript All authors read and approved...
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Isolation, cloning and characterization of the FSH beta gene promoter of the chinook salmon (oncorhynchus tshawytscha)
... the vectors Both the amplified kb fragment and the CAT6 and EGFP plasmid vectors were digested with HindIII and BamHI restriction enzymes (New England Biolabs) according to the manufacturer’s ... Verification and sequence analysis of the kb csFSHβ gene constructs The correct kb csFSHβ-CAT6 vector and kb csFSHβ-EGFP vector constructs were verified by NdeI and NheI restriction enzymes (New England ... (5’-GGTAGACCTTCTCGTAGGACCAC3’) and GSP3 (5’-GGTCATGTTGTTTAGTCGGCAGCCATACCT-3’) The PCR components and cycling parameters are in Table 20 and 21 except that the annealing temperature and extension time are 600C and 30s,...
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Báo cáo y học: " High efficient isolation and systematic identification of human adipose-derived mesenchymal stem cells." ppsx
... potential of adult human mesenchymal stem cells Science 1999, 284:143-147 Pittenger MF, Mosca JD, McIntosh KR: Human mesenchymal stem cells: progenitor cells for cartilage, bone, fat and stroma Curr ... by lipolytic hormones and insulin J Biol Chem 1966, 241:3909-3917 Lange C, Schroeder J, Stute N, Lioznov MV, Zander AR: High-potential human mesenchymal stem cells Stem Cells Dev 2005, 14:70-80 ... of human mesenchymal stem cells Wound Repair Regen 2003, 11:354-360 doi:10.1186/1423-0127-18-59 Cite this article as: Yang et al.: High efficient isolation and systematic identification of human...
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Báo cáo y học: "Identification and isolation of embryonic stem cells in reproductive endocrinology: theoretical protocols for conservation of human embryos derived from in vitro fertilization" ppsx
... facilitate human ESC research and to promote respect for human embryos obtained from clinical reproductive endocrinology practice http://www.tbiomed.com/content/2/1/25 Human embryonic stem cells: ... inner cell mass and trophoblastic cells in blastocysts derived from porcine 4- and 8cell embryos and isolated blastomeres cultured in vitro in the presence or absence of protein and human leukemia ... Marshall VS, Jones JM: Embryonic stem cell lines derived from human blastocysts Science 1998, 282:1145-1147 Fischbach GD, Fischbach RL: Stem cells: science, policy, and ethics J Clin Invest 2004,...
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The derivation, propagation, storage and gene expression of human embryonic stem cells on human feeders
... adult stem cells are the hematopoietic stem cells, mesechymal stem cells and neuronal stem cells Adult stem cells have also been isolated from several other organs such as the brain (neuronal stem ... of cells make up the human body viz., germ cells, somatic cells and stem cells Somatic cells include the bulk of the cells that make up the human adult and each of these cells in their differentiated ... (neuronal stem cells) , skin (epidermal stem cells) , eye (retinal stem cells) and gut (intestinal crypt stem cells) (Spradling et al 2001) However, 15 not all organs and tissues may contain stem cells...
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Stem Cells in Human Reproduction Basic Science and Therapeutic Potential Second Edition pot
... Properties and Potential Benefits of Human Embryonic Stem Cells and Wharton’s Jelly Stem Cells 136 Chui-Yee Fong, Kalamegam Gauthaman, and Ariff Bongso 14 Amniotic Fluid and Placenta Stem Cells 150 ... Antonio Pellicer Stem Cells in Human Reproduction, Second Edition, ISBN: 9780415471718 12 Andrea Borini, Giovanni Coticchio Preservation of Human Oocytes, ISBN 9780415476799 Stem Cells in Human Reproduction ... Vitro–Matured Human Germinal Vesicle Oocytes 226 ¨rn Bjo Heindryckx, Petra De Sutter, and Jan Gerris 21 Derivation and Banking of Human Embryonic Stem Cells for Potential Clinical Use 243 Ana Krtolica and...
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báo cáo hóa học:" MicroRNA and gene expression patterns in the differentiation of human embryonic stem cells" doc
... either hES cells or adult cells (Figure 6, panel B) For miR-106b, miR-92, miR-93, miR-130a and miR-190, the difference in their expression between EB and hES cells and between EB and adult cells were ... miRNA upregulated in hES cells, 31 upregulated in EB cells, and 35 upregulated in adult cells (Figure 2) The 20 miRNAs most highly expressed in hES cells, EB, and adult cells respectively were ... and miR-520b were much higher in hES cells than in either EB or adult cells (Figure 6, panel A) The difference in the expression of miR-200c, miR-302b, and miR-367 between hES cells and EB, and...
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Báo cáo hóa học: " Nutraceutical augmentation of circulating endothelial progenitor cells and hematopoietic stem cells in human subjects" potx
... of human stem cells Stem Cells Dev 2006, 15(1):118-23 77 Shytle RD, Ehrhart J, Tan J, Vila J, Cole M, Sanberg CD, Sanberg PR, Bickford PC: Oxidative stress of neural, hematopoietic, and stem cells: ... growth factor stimulated and not stimulated cells was calculated and compared for different periods before and after intervention Stem- Kine mobilizes CD34 and CD133 Cells Statistics Differences ... infarction [59,60] and stroke [61], are associated with upregulated levels of these cells Given the possibility that both hematopoietic stem cells and EPC may serve as endogenous “repair cells , we sought...
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Báo cáo khoa học: "Human embryonic stem cells and therapeutic cloning" pdf
... repair and transplantation medicine There are several approaches in human clinical trails that employ adult stem cells (such as blood-forming hematopoietic stem cells and cartilage-forming cells) ... adult stem cells is their inability to effectively grow in Human embryonic stem cells and therapeutic cloning culture Therefore, obtaining clinically significant amounts of adult stem cells may ... SCNT-hES-1 cells 61− SSEA-3, SSEA-4, TRA-1-60, TRA-1-81, and Oct-4, but not SSEA-1 (Fig 3) As previously described in monkey [78] and human ES cells [77,58], and mouse SCNT-ES cells [33], SCNT-hES-1 cells...
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