... cryopreservation method for the storage of humanembryonicstemcellsStemCells 2004;22(5):779-89 10 Speicher MR, Ballard SG, Ward DC Karyotyping human chromosomes by combinatorial multi-fluor ... VS, Jones JM Embryonicstem cell lines derived from human blastocysts Science 1998;282(5391):1145-7 Reubinoff BE, Pera MF, Fong CY, Trounson A, Bongso A Embryonicstem cell lines from human blastocysts: ... some cells detached after exposure to low temperature Virtually all of the detached cells were determined to be non-viable with tryphan blue staining (data not shown) All of the detached cells...
... suggest that vascular cells derived from human ES cells may have a potential to be a source for therapeutic vascular regeneration after stroke Abbreviations ES cells: Embryonicstem cells; VEGF-R2: ... from human ES cells could serve as a source for vasculogenesis in order to contribute to therapeutic neovascularization and to neuroprotection in the ischemic brain humanembryonicstemcells ... Characterization of the transplanted vascular cells derived from human ES cells (HES-3) Characterization of the transplanted vascular cells derived from human ES cells (HES-3) A, Flow cytometric analysis...
... The tested adult cells were Human Microvascular Endothelial Cells (HMVEC), Human Umbilical Vein Endothelial Cells (HUVEC), Umbilical Artery Smooth Muscle Cells (UASMC), Normal human astrocytes ... were upregulated in adult cells HMVEC = human microvascular endothelial cells, HUVEC = human umbilical vein endothelial cells, UASMC = umbilical artery smooth muscle cells; NHA = normal astrocyte ... been reported in humanembryonicstem (ES) cells [16,26], mouse ES cells[ 20] or Page 12 of 17 (page number not for citation purposes) Journal of Translational Medicine 2009, 7:20 human EB [17] depending...
... Culture and characterization of humanembryonicstemcellsStemCells Dev 2004, 13, 325-336 18 Drukker M, Benvenisty N The immunogenicity of humanembryonic stem- derived cells Trends Biotechnol 2004, ... approaches in human clinical trails that employ adult stemcells (such as blood-forming hematopoietic stemcells and cartilage-forming cells) A potential advantage of using adult stemcells is that ... adult stemcells is their inability to effectively grow in Humanembryonicstemcells and therapeutic cloning culture Therefore, obtaining clinically significant amounts of adult stemcells may...
... in pluripotent embryonicstemcells and differentiation Nat Rev Mol Cell Biol 2006, 7:540-546 51 Park SH, Kook MC, Kim EY, Park S, Lim JH: Ultrastructure of humanembryonicstemcells and spontaneous ... pluripotent stemcells and embryonicstemcells are distinguished by gene expression signatures Cell Stem Cell 2009, 5:111-123 93 Chin MH, Pellegrini M, Plath K, Lowry WE: Molecular analyses of human ... prevalent in embryonicstemcells and may be mediated by DNA methyltransferase 3a Proc Natl Acad Sci U S A 2000, 97:5237-5242 72 Altun G, Loring JF, Laurent LC: DNA methylation in embryonicstem cells...
... Hours - hr Human- specific vimentin - hVIM Humanembryonicstemcells - hESCs Human ESC-derived chondrogenic cell-engineered cartilage - HCCEC Human palate mesenchymal cell line - HPM Human umbilical ... fibroblasts, stem cells, and genetically modified cells have all been explored for their potential as viable cell sources for cartilage tissue engineering (Chung et al., 2008) Humanembryonicstemcells ... relief of symptoms Stemcells represent a promising cell source for cartilage repair and can be derived from two major sources: mesenchymal stemcells (MSCs) and embryonicstemcells (ESCs) (Lee...
... events underlying human nervous system development (Ben-Nun and Benvenisty, 2006; Dvash et al., 2006) 2.7 Embryonicstemcells and induced pluripotent stemcellsEmbryonicstemcells (ESC) have ... conditioned media (CM) used for daily hESC culture feeding 3.2.3 Humanembryonicstemcells and induced pluripotent stemcellsHumanembryonicstem cell line HES-3 (46, XX) was from ES Cell International ... PUBLICATIONS 161! vii SUMMARY Humanembryonicstemcells (hESC) are pluripotent stemcells that have the unique ability to differentiate into cells of the three germ line lineages Hence,...
... in the course of ex vivo gene therapy 1.1.1 HumanEmbryonicStemCellsHumanembryonicstem (hES) cells are cells derived from the inner cell mass of human blastocysts The hES cell research began ... system DMEM Dulbecco’s modified eagle’s medium EF1α elongation factor 1α eGFP enhanced green fluorescent protein ES cells FBS hES cellsembryonicstemcells fetal bovine serum humanembryonicstem ... humanembryonicstemcells Manuscript in preparation Zeng J, Du J, Zhao Y, Palanisamy N, Wang S Baculoviral vector-mediated transient and stable transgene expression in humanembryonicstem cells...
... adult stemcells are the hematopoietic stem cells, mesechymal stemcells and neuronal stemcells Adult stemcells have also been isolated from several other organs such as the brain (neuronal stem ... (neuronal stem cells) , skin (epidermal stem cells) , eye (retinal stem cells) and gut (intestinal crypt stem cells) (Spradling et al 2001) However, 15 not all organs and tissues may contain stemcells ... cannot be performed in the human Animal embryonicstemcellsEmbryonicstemcells were first derived from certain strains of mice (Evans & Kaufman 1981) Embryonicstem cell lines have been established...
... μ – Mu ° – Degree x CHAPTER 1: GENERAL INTRODUCTION 1.1 Embryonicstemcells The isolation of mouse and humanembryonicstemcells (ES cells) heralded a new era in regenerative medicine, raising ... culture of ICM cells with stem cell-like morphology from human blastocysts (Fig 1.1A) though cultures failed beyond passages The first long-term culture (4-5 months) of humanembryonicstemcells (hESCs) ... T cells 14 Figure 1.5 Haematopoietic development Pluripotent stemcells give rise to multipotent haematopoietic stemcells that differentiate into lymphoid and myeloid progenitors T -cells, B-cells...
... marrow (mesenchymal stem cells, hematopoietic stem cells) and (2) embryonicstem and embryonic germ cells derived from discarded human embryos and germ line stemcells Adult stemcells can be derived ... hESCs-dervied osteogenic cells in bone formation 21 CHAPTER MATERIAL & METHODS 22 2.1 MATERIAL & METHODS Culture and maintenance of HumanEmbryonicStemCellsHumanembryonicstemcells (hESCs) used ... differentiation of hES cells is mandatory 1.5 HumanEmbryonicstemcells As the name suggests, the humanembryonicstemcells are derived from the early embryo These cells have the capacity to self renew and...
... HES3 HumanEmbryonicStemCells Undifferentiated HES4 HumanEmbryonicStemCells SAGE _Embryonic_ stem_ cell_H9_normal_p38_CL_SHES1 LSAGE _Embryonic_ stem_ cell_BG01_normal_p20_CL_SHE19 LSAGE _Embryonic_ stem_ cell_H13_normal_p22_CL_SHE15 ... family Page X Chapter Introduction 1.1 Humanembryonicstemcells 1.1.1 Overview and characteristics of humanembryonicstemcellsEmbryonicstem (ES) cells are isolated from the inner cell mass ... 1.2 Humanembryonicstemcells 1.1.1 Overview and characteristics of humanembryonicstemcells 1.1.2 Regulatory networks and transcription factors in human ES cells 1.1.3 Induced pluripotent stem...
... review and comparison for all three types of stem cells, adult stem cells, embryonicstemcells and induced pluripotent stem cells, embryonicstemcells are inevitable and irreplaceable source ... Biocompatibility 1.2 Stemcells ………………………………………………………………….4 1.2.1 Significance in the use of stemcells 1.2.2 Definition of stemcells 1.2.2.1 Adult stemcells 1.2.2.2 Embryonicstemcells 1.2.2.3 Induced ... the use of adult stemcells either in research or clinical applications 1.2.2.2 Embryonicstem cells: Another type of stemcells by conventional categorization is embryonicstemcells based on...
... RESULTS……………………………………………………… 27 4.1 Characterization of humanembryonicstemcells …………………… 27 4.2 Temperature tolerance of humanembryonicstemcells …………… 30 4.21 Survival rate of hESC after exposure ... established cell lines poorly reflect human physiology Lack of standardization is also a limitation with the use of primary explanted cellsHumanembryonicstemcells (hESCs) have been validated ... culture…………………………… 15 3.13 Differentiation of hESCs…………………………………………………………16 3.2 Characterization of HumanEmbryonicstemcells …………………………… 17 3.21 Real –time reverse transcriptase Polymerase Chain Reaction...
... from humanembryonicstemcells Nat Biotechnol 2001, 19:1129-1133 Kaufman DS, Hanson ET, Lewis RL, Auerbach R, Thomson JA: Hematopoietic colony-forming cells derived from humanembryonicstemcells ... enrichment of cardiomyocytes derived from humanembryonicstemcells Circ Res 2002, 91:501-508 He JQ, Ma Y, Lee Y, Thomson JA, Kamp TJ: Humanembryonicstemcells develop into multiple types of cardiac ... and embryonicstemcells to study human hematopoiesis Curr Opin Biotechnol 2005, 16:510-515 Anderson JS, Bandi S, Kaufman DS, Akkina R: Derivation of normal macrophages from humanembryonic stem...
... Therapeutic potential of embryonicstemcells Blood Rev 2005, 19:321-331 Menendez P, Wang L, Bhatia M: Genetic manipulation of humanembryonicstem cells: A system to study early human development andpotential ... differentiation ofhuman embryonicstemcells Blood 2003, 102:906-915 Wang L, Li L, Menendez P, Cerdan C, Bhatia M: Humanembryonicstemcells maintained in the absence of mouse embryonic fibroblasts ... from humanembryonicstemcells exhibit an embryonic gene expression pattern Blood 2004, 103:4134-4141 Martin CH, Kaufman DS: Synergistic use of adult and embryonicstemcells to study human...
... facilitate human ESC research and to promote respect for human embryos obtained from clinical reproductive endocrinology practice http://www.tbiomed.com/content/2/1/25 Humanembryonicstem cells: ... HA: Embryonic death and the creation of humanembryonicstemcells J Clin Invest 2004, 114:1184-1186 Voullaire L, Slater H, Williamson R, Wilton L: Chromosome analysis of blastomeres from human ... Swiergiel JJ, Marshall VS, Jones JM: Embryonicstem cell lines derived from human blastocysts Science 1998, 282:1145-1147 Fischbach GD, Fischbach RL: Stem cells: science, policy, and ethics J...
... of regulation of NSC differentiation 1.1.5.2 Pluripotent stem cell-derived NSCs Humanembryonicstemcells (hESCs), which are pluripotent cells derived from the inner cell mass of blastocyst-stage ... “Maintenance of HumanEmbryonicStemCells in mTeSRTM1” (STEMCELL Technologies Inc.) In brief, H1 hESCs were maintained on BD matrigel hESC-qualified matrix (BD Biosciences) with mTeSRTM1 medium (STEMCELL ... HUMANEMBRYONICSTEM CELL-DERIVED NEURAL STEM CELLS: DERIVATION, DIFFERENTIATION AND MICRORNA REGULATION KWANG WEI XIN...