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human embryonic stem cells

Báo cáo y học:

Báo cáo y học: "Low temperature tolerance of human embryonic stem cells"

Y học thưởng thức

... cryopreservation method for the storage of human embryonic stem cells Stem Cells 2004;22(5):779-89 10 Speicher MR, Ballard SG, Ward DC Karyotyping human chromosomes by combinatorial multi-fluor ... VS, Jones JM Embryonic stem cell lines derived from human blastocysts Science 1998;282(5391):1145-7 Reubinoff BE, Pera MF, Fong CY, Trounson A, Bongso A Embryonic stem cell lines from human blastocysts: ... some cells detached after exposure to low temperature Virtually all of the detached cells were determined to be non-viable with tryphan blue staining (data not shown) All of the detached cells...
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báo cáo hóa học:

báo cáo hóa học:" Transplantation of vascular cells derived from human embryonic stem cells contributes to vascular regeneration after stroke in mice" docx

Hóa học - Dầu khí

... suggest that vascular cells derived from human ES cells may have a potential to be a source for therapeutic vascular regeneration after stroke Abbreviations ES cells: Embryonic stem cells; VEGF-R2: ... from human ES cells could serve as a source for vasculogenesis in order to contribute to therapeutic neovascularization and to neuroprotection in the ischemic brain human embryonic stem cells ... Characterization of the transplanted vascular cells derived from human ES cells (HES-3) Characterization of the transplanted vascular cells derived from human ES cells (HES-3) A, Flow cytometric analysis...
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báo cáo hóa học:

báo cáo hóa học:" MicroRNA and gene expression patterns in the differentiation of human embryonic stem cells" doc

Hóa học - Dầu khí

... The tested adult cells were Human Microvascular Endothelial Cells (HMVEC), Human Umbilical Vein Endothelial Cells (HUVEC), Umbilical Artery Smooth Muscle Cells (UASMC), Normal human astrocytes ... were upregulated in adult cells HMVEC = human microvascular endothelial cells, HUVEC = human umbilical vein endothelial cells, UASMC = umbilical artery smooth muscle cells; NHA = normal astrocyte ... been reported in human embryonic stem (ES) cells [16,26], mouse ES cells[ 20] or Page 12 of 17 (page number not for citation purposes) Journal of Translational Medicine 2009, 7:20 human EB [17] depending...
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báo cáo hóa học:

báo cáo hóa học:" Human embryonic stem cells hemangioblast express HLA-antigens" pot

Hóa học - Dầu khí

... MHC proteins in human embryonic stem cells Proc Natl Acad Sci USA 2002, 99(15):9864-9 Li L, Baroja ML, Majumdar A, Chadwick K, Rouleau A, Gallacher L, et al.: Human embryonic stem cells possess ... molecules on murine embryonic stem cells Biol Reprod 1997, 57(3):561-8 Bonde S, Zavazava N: Immunogenicity and engraftment of mouse embryonic stem cells in allogeneic recipients Stem Cells 2006, 24(10):2192-201 ... susceptible to immune rejection than adult cells Stem Cells 2006, 24(2):221-9 Koch CA, Geraldes P, Platt JL: Immunosuppression by embryonic stem cells Stem Cells 2008, 26(1):89-98 Fabricius D, Bonde...
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Báo cáo khoa học:

Báo cáo khoa học: "Human embryonic stem cells and therapeutic cloning" pdf

Báo cáo khoa học

... Culture and characterization of human embryonic stem cells Stem Cells Dev 2004, 13, 325-336 18 Drukker M, Benvenisty N The immunogenicity of human embryonic stem- derived cells Trends Biotechnol 2004, ... approaches in human clinical trails that employ adult stem cells (such as blood-forming hematopoietic stem cells and cartilage-forming cells) A potential advantage of using adult stem cells is that ... adult stem cells is their inability to effectively grow in Human embryonic stem cells and therapeutic cloning culture Therefore, obtaining clinically significant amounts of adult stem cells may...
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báo cáo khoa học:

báo cáo khoa học: "Epigenomics of human embryonic stem cells and induced pluripotent stem cells: insights into pluripotency and implications for disease" potx

Báo cáo khoa học

... in pluripotent embryonic stem cells and differentiation Nat Rev Mol Cell Biol 2006, 7:540-546 51 Park SH, Kook MC, Kim EY, Park S, Lim JH: Ultrastructure of human embryonic stem cells and spontaneous ... pluripotent stem cells and embryonic stem cells are distinguished by gene expression signatures Cell Stem Cell 2009, 5:111-123 93 Chin MH, Pellegrini M, Plath K, Lowry WE: Molecular analyses of human ... prevalent in embryonic stem cells and may be mediated by DNA methyltransferase 3a Proc Natl Acad Sci U S A 2000, 97:5237-5242 72 Altun G, Loring JF, Laurent LC: DNA methylation in embryonic stem cells...
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Differentiation and derivation of lineage committed chondroprogenitors and chondrogenic cells from human embryonic stem cells for cartilage tissue engineering and regeneration

Differentiation and derivation of lineage committed chondroprogenitors and chondrogenic cells from human embryonic stem cells for cartilage tissue engineering and regeneration

Cao đẳng - Đại học

... Hours - hr Human- specific vimentin - hVIM Human embryonic stem cells - hESCs Human ESC-derived chondrogenic cell-engineered cartilage - HCCEC Human palate mesenchymal cell line - HPM Human umbilical ... fibroblasts, stem cells, and genetically modified cells have all been explored for their potential as viable cell sources for cartilage tissue engineering (Chung et al., 2008) Human embryonic stem cells ... relief of symptoms Stem cells represent a promising cell source for cartilage repair and can be derived from two major sources: mesenchymal stem cells (MSCs) and embryonic stem cells (ESCs) (Lee...
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Establishment of autologous culture systems for human embryonic stem cells

Establishment of autologous culture systems for human embryonic stem cells

Cao đẳng - Đại học

... of stem cells by derivation source Category Embryonic stem cells Embryonic germ cells Fetal stem cells Umbilical cord stem cells Umbilical cord blood stem cells Umbilical cord matrix stem cells ... (Sorgner, 2007) Embryonic stem cells Mesenchymal stem cells, Hematopoietic stem cells Oligopotent Can differentiate into only a few cells (Sorgner, 2007) Lymphoid stem cells, Myeloid stem cells Unipotent ... embryonic germ cells, fetal stem cells, umbilical cord stem cells, adult stem cells and induced pluripotent stem (iPS) cells (Ariff Bongso, 2005, see Table 2) Each type of stem cells holds its...
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Role of sonic hedgehog signalling in human embryonic stem cells and its neural derivatives

Role of sonic hedgehog signalling in human embryonic stem cells and its neural derivatives

Cao đẳng - Đại học

... events underlying human nervous system development (Ben-Nun and Benvenisty, 2006; Dvash et al., 2006) 2.7 Embryonic stem cells and induced pluripotent stem cells Embryonic stem cells (ESC) have ... conditioned media (CM) used for daily hESC culture feeding 3.2.3 Human embryonic stem cells and induced pluripotent stem cells Human embryonic stem cell line HES-3 (46, XX) was from ES Cell International ... PUBLICATIONS 161! vii SUMMARY Human embryonic stem cells (hESC) are pluripotent stem cells that have the unique ability to differentiate into cells of the three germ line lineages Hence,...
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Baculovirus mediated genetic modification of human embryonic stem cells

Baculovirus mediated genetic modification of human embryonic stem cells

Tổng hợp

... in the course of ex vivo gene therapy 1.1.1 Human Embryonic Stem Cells Human embryonic stem (hES) cells are cells derived from the inner cell mass of human blastocysts The hES cell research began ... system DMEM Dulbecco’s modified eagle’s medium EF1α elongation factor 1α eGFP enhanced green fluorescent protein ES cells FBS hES cells embryonic stem cells fetal bovine serum human embryonic stem ... human embryonic stem cells Manuscript in preparation Zeng J, Du J, Zhao Y, Palanisamy N, Wang S Baculoviral vector-mediated transient and stable transgene expression in human embryonic stem cells...
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The derivation, propagation, storage and gene expression of human embryonic stem cells on human feeders

The derivation, propagation, storage and gene expression of human embryonic stem cells on human feeders

Cao đẳng - Đại học

... adult stem cells are the hematopoietic stem cells, mesechymal stem cells and neuronal stem cells Adult stem cells have also been isolated from several other organs such as the brain (neuronal stem ... (neuronal stem cells) , skin (epidermal stem cells) , eye (retinal stem cells) and gut (intestinal crypt stem cells) (Spradling et al 2001) However, 15 not all organs and tissues may contain stem cells ... cannot be performed in the human Animal embryonic stem cells Embryonic stem cells were first derived from certain strains of mice (Evans & Kaufman 1981) Embryonic stem cell lines have been established...
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Directed differentiation of human embryonic stem cells into haematopoietic and definitive endodermal lineages

Directed differentiation of human embryonic stem cells into haematopoietic and definitive endodermal lineages

Tổng hợp

... μ – Mu ° – Degree x CHAPTER 1: GENERAL INTRODUCTION 1.1 Embryonic stem cells The isolation of mouse and human embryonic stem cells (ES cells) heralded a new era in regenerative medicine, raising ... culture of ICM cells with stem cell-like morphology from human blastocysts (Fig 1.1A) though cultures failed beyond passages The first long-term culture (4-5 months) of human embryonic stem cells (hESCs) ... T cells 14 Figure 1.5 Haematopoietic development Pluripotent stem cells give rise to multipotent haematopoietic stem cells that differentiate into lymphoid and myeloid progenitors T -cells, B-cells...
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IN VIVO  EX VIVO OSTEOGENESIS OF HUMAN EMBRYONIC STEM CELLS

IN VIVO EX VIVO OSTEOGENESIS OF HUMAN EMBRYONIC STEM CELLS

Tổng hợp

... marrow (mesenchymal stem cells, hematopoietic stem cells) and (2) embryonic stem and embryonic germ cells derived from discarded human embryos and germ line stem cells Adult stem cells can be derived ... hESCs-dervied osteogenic cells in bone formation 21 CHAPTER MATERIAL & METHODS 22 2.1 MATERIAL & METHODS Culture and maintenance of Human Embryonic Stem Cells Human embryonic stem cells (hESCs) used ... differentiation of hES cells is mandatory 1.5 Human Embryonic stem cells As the name suggests, the human embryonic stem cells are derived from the early embryo These cells have the capacity to self renew and...
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Transcriptome study of human embryonic stem cells and knockdown study of a pluripotency marker, LIN28

Transcriptome study of human embryonic stem cells and knockdown study of a pluripotency marker, LIN28

Thạc sĩ - Cao học

... HES3 Human Embryonic Stem Cells Undifferentiated HES4 Human Embryonic Stem Cells SAGE _Embryonic_ stem_ cell_H9_normal_p38_CL_SHES1 LSAGE _Embryonic_ stem_ cell_BG01_normal_p20_CL_SHE19 LSAGE _Embryonic_ stem_ cell_H13_normal_p22_CL_SHE15 ... family Page X Chapter Introduction 1.1 Human embryonic stem cells 1.1.1 Overview and characteristics of human embryonic stem cells Embryonic stem (ES) cells are isolated from the inner cell mass ... 1.2 Human embryonic stem cells 1.1.1 Overview and characteristics of human embryonic stem cells 1.1.2 Regulatory networks and transcription factors in human ES cells 1.1.3 Induced pluripotent stem...
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Human embryonic stem cells as a cellular model for osteogenesis in implant testing and drug discovery

Human embryonic stem cells as a cellular model for osteogenesis in implant testing and drug discovery

Kỹ thuật - Công nghệ

... review and comparison for all three types of stem cells, adult stem cells, embryonic stem cells and induced pluripotent stem cells, embryonic stem cells are inevitable and irreplaceable source ... Biocompatibility 1.2 Stem cells ………………………………………………………………….4 1.2.1 Significance in the use of stem cells 1.2.2 Definition of stem cells 1.2.2.1 Adult stem cells 1.2.2.2 Embryonic stem cells 1.2.2.3 Induced ... the use of adult stem cells either in research or clinical applications 1.2.2.2 Embryonic stem cells: Another type of stem cells by conventional categorization is embryonic stem cells based on...
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Human embryonic stem cells for genotoxicity testing

Human embryonic stem cells for genotoxicity testing

Tổng hợp

... RESULTS……………………………………………………… 27 4.1 Characterization of human embryonic stem cells …………………… 27 4.2 Temperature tolerance of human embryonic stem cells …………… 30 4.21 Survival rate of hESC after exposure ... established cell lines poorly reflect human physiology Lack of standardization is also a limitation with the use of primary explanted cells Human embryonic stem cells (hESCs) have been validated ... culture…………………………… 15 3.13 Differentiation of hESCs…………………………………………………………16 3.2 Characterization of Human Embryonic stem cells …………………………… 17 3.21 Real –time reverse transcriptase Polymerase Chain Reaction...
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Báo cáo y học:

Báo cáo y học: " Human embryonic stem cell (hES) derived dendritic cells are functionally normal and are susceptible to HIV-1 infection" pot

Báo cáo khoa học

... from human embryonic stem cells Nat Biotechnol 2001, 19:1129-1133 Kaufman DS, Hanson ET, Lewis RL, Auerbach R, Thomson JA: Hematopoietic colony-forming cells derived from human embryonic stem cells ... enrichment of cardiomyocytes derived from human embryonic stem cells Circ Res 2002, 91:501-508 He JQ, Ma Y, Lee Y, Thomson JA, Kamp TJ: Human embryonic stem cells develop into multiple types of cardiac ... and embryonic stem cells to study human hematopoiesis Curr Opin Biotechnol 2005, 16:510-515 Anderson JS, Bandi S, Kaufman DS, Akkina R: Derivation of normal macrophages from human embryonic stem...
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Báo cáo y học:

Báo cáo y học: " Derivation of normal macrophages from human embryonic stem (hES) cells for applications in HIV gene therapy" pps

Báo cáo khoa học

... Therapeutic potential of embryonic stem cells Blood Rev 2005, 19:321-331 Menendez P, Wang L, Bhatia M: Genetic manipulation of human embryonic stem cells: A system to study early human development andpotential ... differentiation ofhuman embryonic stem cells Blood 2003, 102:906-915 Wang L, Li L, Menendez P, Cerdan C, Bhatia M: Human embryonic stem cells maintained in the absence of mouse embryonic fibroblasts ... from human embryonic stem cells exhibit an embryonic gene expression pattern Blood 2004, 103:4134-4141 Martin CH, Kaufman DS: Synergistic use of adult and embryonic stem cells to study human...
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Báo cáo y học:

Báo cáo y học: "Identification and isolation of embryonic stem cells in reproductive endocrinology: theoretical protocols for conservation of human embryos derived from in vitro fertilization" ppsx

Báo cáo khoa học

... facilitate human ESC research and to promote respect for human embryos obtained from clinical reproductive endocrinology practice http://www.tbiomed.com/content/2/1/25 Human embryonic stem cells: ... HA: Embryonic death and the creation of human embryonic stem cells J Clin Invest 2004, 114:1184-1186 Voullaire L, Slater H, Williamson R, Wilton L: Chromosome analysis of blastomeres from human ... Swiergiel JJ, Marshall VS, Jones JM: Embryonic stem cell lines derived from human blastocysts Science 1998, 282:1145-1147 Fischbach GD, Fischbach RL: Stem cells: science, policy, and ethics J...
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Human embryonic stem cell derived neural stem cells derivation, differentiation and MicroRNA regulation

Human embryonic stem cell derived neural stem cells derivation, differentiation and MicroRNA regulation

Cao đẳng - Đại học

... of regulation of NSC differentiation 1.1.5.2 Pluripotent stem cell-derived NSCs Human embryonic stem cells (hESCs), which are pluripotent cells derived from the inner cell mass of blastocyst-stage ... “Maintenance of Human Embryonic Stem Cells in mTeSRTM1” (STEMCELL Technologies Inc.) In brief, H1 hESCs were maintained on BD matrigel hESC-qualified matrix (BD Biosciences) with mTeSRTM1 medium (STEMCELL ... HUMAN EMBRYONIC STEM CELL-DERIVED NEURAL STEM CELLS: DERIVATION, DIFFERENTIATION AND MICRORNA REGULATION KWANG WEI XIN...
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