host resistance to listeria monocytogenes infection is impaired by cold restraint treatment
Báo cáo y học: " Persistent resistance to HIV-1 infection in CD4 T cells from exposed uninfected Vietnamese individuals is mediated by entry and post-entry blocks" potx
... [9-11] This resistance was usually restricted to HIV-1 isolates using the CCR5 chemokine receptor (R5 isolates) to enter target cells [12-14] Invalidating mutations in the CCR5 gene confer resistance ... exposed to HIV-1 and yet remain uninfected (exposed uninfected individuals, EU) are likely to be naturally resistant to infection Relative resistance of CD4 T cells and/or macrophages to HIV1 infection ... cells might be due to missing or defective cell factor(s) necessary for viral replication, rather than to antiviral molecules Strong CD4 T cell resistance to HIV-1 infection is a highly unusual...
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Báo cáo hóa học: " Glycerol conversion to 1, 3-Propanediol is enhanced by the expression of a heterologous alcohol dehydrogenase gene in Lactobacillus reuteri" ppt
... phosphate is channeled more towards ethanol production (Figure 5) This is most likely reflected as a shift in metabolism from acetate to ethanol production, resulting in reduced ATP synthesis The ... terminator, sh71rep replication origin for Lactobacillus, ermL erythromycin -resistance marker, PssIP and PorfX inducible promoters, sppK and sppR histidine protein kinase and response regulator ... flux from central carbon metabolism towards 1, 3-PD synthesis should be complemented by adequate levels of enzymes and cofactors involved in the pathway In this work, we have expressed an E coli...
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Báo cáo y học: "β Susceptibility to collagen-induced arthritis is modulated by TGFβ responsiveness of T cells" ppt
... technical assistance References 10 11 12 13 14 15 16 Anthony DD, Haqqi TM: Collagen-induced arthritis in mice: an animal model to study the pathogenesis of rheumatoid arthritis Clin Exp Rheumatol 1999, ... clinical and histological arthritis score with an increased production of Th1 cytokines by draining lymph node cells of transgenic mice These findings indicate the importance of regulatory effects ... chronic phase of arthritis, and grades ranging from to were allotted to each limb: grade 0, no visible abnormalities; grade 1, mild redness or swelling of wrist or up to three inflamed digits;...
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Báo cáo y học: "The contact-mediated response of peripheral-blood monocytes to preactivated T cells is suppressed by serum factors in rheumatoid arthritis" ppt
... to contribute to the abundant TNF-α production seen in this disease, the role of peripheral monocytes from patients with RA in this interaction has not yet been investigated To address this issue, ... the inhibitory activity, whereas increasing the temperature to 70 or 95°C resulted in a loss of that inhibitory activity Thus, the inhibitory activity is due to heat-labile factors Discussion ... major contributors to the TNFα load in RA RA, where the molecule seems to act as an inhibitory regulator of cytokine production [47] In its absence, one would expect cytokines to reach extremely...
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Báo cáo y học: " Intracellular HIV-1 Gag localization is impaired by mutations in the nucleocapsid zinc fingers" pdf
... the PM together with the gRNA (fraction 1) By immunofluorescence microscopy, Gag was present in patches at the PM and in the cytoplasm, ressembling the gRNA pattern by FISH (see IF and FISH pictures, ... binds to the cellular membrane by the Nterminal myristoylated domain and stretches of basic residues of the matrix domain (reviewed in [31,32], also To confirm the multiple roles played by NC ... [26] and stored at 4°C in 70% ethanol Detection of the HIV-1 genomic RNA was performed by FISH [26] with a Cy3-conjugated oligonucleotide (GagHIVCy3), corresponding to position 1524 to 1563 of...
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báo cáo khoa học: " Resistance to Plasmopara viticola in a grapevine segregating population is associated with stilbenoid accumulation and with specific host transcriptional responses" doc
... is swiftly blocked by a combination of constitutive and post -infection resistance mechanisms [6,7] Indeed, resistant Vitis species may benefit from a higher level of constitutive resistance to ... individuals to be distributed in nine classes ranging from total resistance to total susceptibility (Additional file 1) The parents in all three years, one confirmed to be partially resistant (M) ... Cipriani G, Morgante M, Testolin R, Di Gaspero G: Resistance to Plasmopara viticola in grapevine ‘Bianca’ is controlled by a major dominant gene causing localised necrosis at the infection site Theor...
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Báo cáo khoa học: "RAGE: Exacting a toll on the host in response to polymicrobial sepsis and Listeria monocytogenes" pps
... acute, infection since SCID (severe combined immunodeficiency disease) mice survive early listeriosis normally, but ultimately this infection is lethal due to long-term failure to clear the organism ... for host survival to Listeria as mice deficient in Myd88 are exquisitely vulnerable to this bacterium [8] Interestingly, mice deficient in either TLR-2 or TLR-4 display relatively normal resistance ... mediates tissue damage initiated in response to overwhelming bacterial infection Future studies must dissect the precise mechanisms by which RAGE exacts a heavy toll on the host during efforts to combat...
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báo cáo hóa học:" Protective versus pathogenic anti-CD4 immunity: insights from the study of natural resistance to HIV infection" potx
... remarkable amounts of self-epitopes This second mechanism was found capable to break tolerance and trigger cytotoxic T cell-mediated autoreactivity towards several autoantigens, such as myosin, ... HIV-2 is comparable to that seen in HIV-1 infection [3] In this scenario, the immune response to self antigens has often been alleged to play a detrimental role, by acting as an effector mechanism ... autoimmunity to CD4 in HIV-1 infected patients is supported by several mechanisms associated with the generation of cryptic epitopes and to the activation of T cells not previously deleted by...
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Báo cáo y học: "Local hyperhemia to heating is impaired in secondary Raynaud''''s phenomenon" pptx
... as a tool to assess microvascular function during therapy in diseases such as atherosclerosis [16,17] This post-occlusive hyperhemia is due both to metabolic and endothelium derived factors We ... controls by an initial peak within the first 10 minutes followed by a nadir and a final rise to a second peak that continues as a sustained plateau Maximal skin blood flow is achieved by heating to ... sclerosis (SSc) and rheumatoid arthritis (a) Patients with systemic sclerosis (SSc), primary Rayarthritis naud's phenomenon (RP) and healthy controls (b) Patients with rheumatoid arthritis (RA)...
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Báo cáo y học: " CXCR2 is critical for dsRNA-induced lung injury: relevance to viral lung infection" pps
... edema; microvascular permeability; mRNA; ELISA analysis; and histopathological and immunohistochemical analysis by fixing in 4% paraformaldehyde at 25 to 30 cm H2O pressure and embedding in paraffin ... inflammation and injury to mimic an acute viral infection and thus dissect the mechanisms related to this process The putative receptor for dsRNA has been identified as Toll-like receptor (TLR3), thus ... family of toll-like receptors (TLRs) that aid the host in combating infections when microbial pathogens bind to their specific toll-like receptor to trigger NFκB and downstream generation of cytokines...
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báo cáo khoa học: " Systemic acquired resistance in soybean is regulated by two proteins, Orthologous to Arabidopsis NPR1" ppt
... marker, is needed in non-inoculated systemic tissue to provide resistance against secondary infection To determine if pathogenic infection can also lead to GmPR1 expression in systemic tissues, ... defense mechanisms to protect themselves from pathogen attack One of the most common inducible defense mechanisms is systemic acquired resistance (SAR) SAR can be triggered by infection with ... pathogenesis-related genes by INA or infection in Arabidopsis npr1-1 mutant carrying GmNPR1 genes Induction Induction of the pathogenesis-related genes by INA or infection in Arabidopsis npr1-1...
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báo cáo khoa học: " Sgt1, but not Rar1, is essential for the RB-mediated broad-spectrum resistance to potato late blight" pptx
... potato This result will need to be confirmed by multiple completely silenced Sgt1 lines Requirement of the RAR1 and SGT1 proteins in disease resistance A role of the RAR1 protein in disease resistance ... potato lines may be sufficient to trigger the resistance pathway Many previous studies confirmed the requirement of the SGT1 protein in resistance mediated by both NB-LRR and Pto-kinase type resistance ... Kamoun S: Nonhost resistance to Phytophthora: novel prospects for a classical problem Curr Opin Plant Biol 2001, 4:295-300 Niederhauser JS, Millis WR: Resistance of Solanum species to Phytophthora...
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Báo cáo khoa học: "Pro/con debate: Antifungal prophylaxis is important to prevent fungal infection in patients with acute necrotizing pancreatitis receiving broad-spectrum antibiotics" docx
... antifungal prophylaxis in acute Con: Prevalence and risks of fungal colonisation of pancreatic necrosis Saurabh Jamdar and Ajith K Siriwardena Fungal colonisation of pancreatic tissue is a feared complication ... antifungal prophylaxis with a triazole compound in any patients receiving antibacterial prophylaxis This prophylaxis may not only prevent infection of necrotic pancreatic tissue, but also delay ... surgical debridement of infected necrosis Several small randomised trials of antibiotic prophylaxis in severe acute pancreatitis [13-15] together with a meta-analysis [16] and a Cochrane systematic...
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Báo cáo y học: " Dendritic cell-mediated HIV-1 transmission to T cells of LAD-1 patients is impaired due to the defect in LFA-1" docx
... LFA-1 negative T cells is impaired in comparison to healthy controls Furthermore, HIV-1 transmission to T cells isolated from the unique LAD-1/variant patient is impaired too, meaning that not ... DC-mediated HIV-1 transmission to LFA-1 negative T cells is impaired DC-mediated HIV-1 transmission to LFA-1 negative T cells is impaired DC were incubated with HIV-1, followed by washing to remove unbound ... followed by CA-p24 ELISA [8,10] In accordance with this, we now show that DCmediated transmission to LFA-1-negative T cells from LAD-1 patients is severely impaired Normally, LFA-1 is activated by...
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Báo cáo y học: "Sustained virological and biochemical responses to lamivudine and adefovir dipivoxil combination in a chronic hepatitis B infection despite mutations conferring resistance to both drugs" potx
... either by sequencing analysis or both the line probe assay and ARMS This resistance profile persisted at M55 but the rtN236T-mutated strain proportion slightly decreased (from 34% at M48 to 27% ... [20] Conclusion This case illustrates that rtM204V/I, rtA181V, and rtN236T resistance mutations can coexist in a patient We conclude that given an unfavorable resistance profile this case suggests ... phenotypical resistance of this strain to both adefovir and lamivudine Thus, the concomitant association of rtM204V/I/S and rtN236T is very rare and has even been described as mutually exclusive by Osiowy...
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Báo cáo sinh học: " Quantitative trait loci for resistance to trichostrongylid infection in Spanish Churra sheep" docx
... However, this hypothesis is challenged by the fact that overall immunity has been successfully achieved through selection for acquired resistance rather than via resistance to primary exposure to worms ... processes that lead to nematode resistance [31] In order to compare our QTL analysis results with chromosomal regions previously identified in sheep in relation to parasite resistance traits, we ... analysis for the four traits related to parasite resistance considered in this experiment In this genome scan a total of 182 markers (181 microsatellites and SNP) distributed along the 26 ovine autosomes...
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đồ án tốt nghiệp tình hình nhiễm vi khuẩn listeria monocytogenes trên dưa muối chua
... Bacillales Họ: Listeriaceae Giống: Listeria Lúc đầu Listeria biết đến với hay hai loài chủ yếu Listeria monocytogenes, sau xác định loài gồm: Listeria monocytogenes, Listeria innocua, Listeria seeligeri, ... Riboflavin Streptococcus faecalis Lactobacillus casei Acid pantothenic Leuconostoc mesenteroides Streptococcus faecalis Streptococcus lactic Streptococcus faecalis Lactobacillus casei Lactobacillus ... KHUẨN Listeria monocytogenes: 1.4.1 Lịch sử phát hiện: Listeria monocytogenes lần mô tả E.G.D Murray vào năm 1926 dựa sáu trường hợp đột tử trẻ Murray giới thiệu Vi khuẩn Listeria monocytogenes...
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Differential host immune responses in BALB c and C57BL 6 mice to burkholderia pseudomallei infection
... asymptomatic, chronic suppurative infection to potentially fatal septicemia The aim of this project is to study the host responses and factors which contribute to resistance or susceptibility in two ... IL-18, and more anti-inflammatory cytokine (IL-10) compared to the splenocytes of C57BL/6 mice This study suggests that resistance to infection lies in the innate ability to control the bacterial ... 2000) Risk of relapse is related to adherence to treatment and the initial extent of disease, but not to the underlying condition (Chaowagul et al., 1993) B pseudomallei strains resistant to chloramphenicol,...
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Roles of siRNAs and miRNAs in host responses to virus infection identification and characterization of a novel viral suppressor of RNA silencing
... encoded by these genes are similar to the tomato RdRP The proposed role of the cellular RdRP in Arabidopsis is to convert an aberrant single-stranded (ss) RNA of a transgene into a dsRNA to trigger ... Thus, the studies by plant scientists led to the discovery of a completely novel RNA-guided gene regulatory mechanism that is universally conserved among many eukaryotic organisms including mammals ... III), to initiate RNAi (Parrish and Fire, 2001; Tabara et al., 2002) RDE-1 is not necessary for gene silencing induced by short antisense RNAs (Tabara et al., 2002) This suggests that RDE-1 together...
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Vi khuẩn listeria monocytogenes
... trước dùng Kết luận L .monocytogenes, vấn đề y tế công cộng Vi khuẩn L .monocytogenes sản xuất độc tố, gây bệnh cách sinh sản phát triển thể Nhiệt độ tối hảo để vi khuẩn L .monocytogenes phát triển ... cộng nhiều quốc gia./ Tham khảo: -CDC Listeriosis http://www.cdc.gov/ncidod/dbmd/diseaseinfo/listeriosis_g.htm -CFIA Faits sur la salubrité des aliments: Listéria http://www.inspection.gc.ca/scripts/priimppage.php?lang=f&ser=www.inspection ... khuẩn khác Các nhà khoa học ước lượng có 5% dân chúng có mang vi khuẩn L .monocytogenes người không mắc bệnh Bệnh Listériosis nguồn gốc từ thực phẩm thật vấn đề y tế công cộng Chính vi khuẩn nầy...
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