... undergo an important conformational change that brings loops A and B into close contact In the present work, and in order to obtain more information on the mechanism by which adenine restores the ... preincubated for 10 with MgCl2 before addition of adenine and application of pressure In the second, ADHR1 was preincubated with adenine for 10 before addition of MgCl2 and application of pressure ... 38, 381–393 Gilbert W (1986) The RNA world Nature 319, 618 Gesteland RF, Cech TR & Atkins JF (1999) In The RNA World Cold Spring Harbor Laboratory Press, New York, NY ´ Salehi-Ashtiani K, Luptak...
... stored at C All samples were centrifuged at 20 000 g during at 15 C prior to the SANS experiments High- pressure cell During the SANS experiments the protein solutions were contained in a high- pressure ... information is important in order to understand the properties of proteins under pressure In the future it would be benecial to perform SANS measurements at higher pressures in order to determine the ... 42 Pressure eects on azidometmyoglobin (Eur J Biochem 269) 4737 A high- pressure laser ash photolysis study J Biol Chem 264, 1889618901 Uchida, T., Ishimori, K & Morishima, I (2000) Unusual pressure...
... decrease or increase with increasing pressure As an example, Fig demonstrates the results for 1R-camphor, where the rate increases with pressure, and for norbornane, where the rate decreases with pressure ... cm3Æmol)1 for the CO binding in the 1R-camphor-bound P450cam In contrast, the activation volumes are positive for the norcamphor-bound as well as for the norbornane-bound proteins (% +8 cm3Æmol)1 for ... 1R-camphor (0.97); norcamphor (0.71) and norbornane (0.93) 100mM potassium phosphate buffer, pH 7.3, 10% (w/w) glycerol, substrate dissociation constants [3]: norcamphor (345 lM), norbornane (47 lM),...
... at 290 nm for a 1% solution of 6.6 for ATP-G-actin [20] Highpressure apparatus Highpressure devices used for this study consisted of a thermostated highpressure vessel equipped with sapphire ... of the pressure- induced denaturation of eATP G-actin (or eADP±F-actin), samples were kept at elevated pressures, and the changes in the ¯uorescence intensity under pressure were monitored The ... NaN3 The pressure was maintained for after reaching the indicated pressure as indicated by the arrows ¯uorescence evidently corresponded to the dissociation of eADP bound to F-actin For G-actin...
... these high pressures Hydrogen±tritium exchange under highpressure Effects of highpressure on irreversible tritium incorporation into Na+/K+-ATPase are shown in Fig Tritium incor- Fig Eect of pressure ... to 345 nm with increasing pressure, up to 220 MPa, and then decreased for pressures of 220 MPa or higher, being independent on ligands, Na+ and /or K+ However, the ¯uorescence intensity showed ... MPa or higher and FITC becomes hydrophilic with increasing pressure To examine reversibility of pressure- induced ¯uorescence changes, BIPM and FITC ¯uorescence intensities were measured after pressure...
... for the aminoacylation reaction of tRNA at highpressure remains unknown Recently, we obtained new information about the conformation of tRNA at elevated pressure [26] It is known that highpressure ... (Cl-Tyr), l-ornithine (l-Orn), d-ornithine (d-Orn), 3,4-dihydroxyphenylalanine (DOPA) and p-chloro-phenylalanine (Cl-Phe) was analysed using PAGE (Fig 1A,B) The amounts of amino acid incorporated ... Phe at highpressure without the need for a specific aa-tRNA synthetase Aminoacylation occurred only at the 3¢-end of tRNA [22] and pressure- aminoacylated Phe–tRNAPhe was a normal substrate for peptide...
... resected femoral neck With more controlled and reduced cement leakage the pressure drops more gradually allowing the highpressure to be retained for longer The time with high cementation pressure ... Discussion The modified pressuriser generates longer highpressure durations in the femoral model The pressure was homogenous within the femoral cavity for both pressurisers Increases in cement pressurisation ... penetration [6,7,11-16] while constant highpressure benefits high viscosity cement penetration whether in a constrained cavity such as the femur [17] or a more open area such as the acetabulum...
... from the high- pressure pump) or the waterjet exiting the nozzle For the purposes of the current discussion, we will assume that this component can either be neglected or has been filtered beforehand ... {classification} (15) LABORATORY DATA Mine detection algorithms were tested both using laboratory data and field data Laboratory data was used to test the algorithms’ ability to detect when an object was ... our laboratory tests shown earlier, preliminary studies in the laboratory indicate that the angle should not prevent detection and discrimination The more shallow firing angle was required for other...
... [6], the root pressure probe [18], the potometer [29], the negative pressure flow system for root sections [14], the highpressure flowmeter (HPFM) [25, 27] Each method was developed for a particular ... each point corresponds to one record For the transient mode, pressure was increased at a rate of 5–8 kPa s–1 and kr was estimated from the slope of the last points For dynamic mode, pressure was ... on Quercus robur L Acorns were sowed in February in 5-liter pots filled with sandy soil (B-horizon) from the Mondon forest (North-East of France) and seedlings were grown for months in a greenhouse...
... at 0.2 MPa pressure for or h Initially, flow rate was high but declined gradually The initially high flow rate was attributed to negative leaf water potentials, ψ However, after or h the leaf ... cut = Prior to connecting shoots to the highpressure flowmeter described below, all cut surfaces were shaved with a razor blade to remove blockage of cut vessels by cell-wall fragments formed by ... drought, may be important for a better understanding of adaptation to drought or However, differences in stem resistanccould account for differences in growth rate under mild drought Higher stem resistances...
... older and had more often an ECOG performance score more than At multivariate analysis only treatment with chemo-radiation was left as a prognostic factor for OS (P = 0.024) The HR for OS of sequential ... with chemoradiation Performance status and age are well known as prognostic factors for survival [21,35-37] After correction for treatment modality, the significance of age and performance status ... influence of repopulation [44] Several other studies report about improved results while shortening the OTT, for radiotherapy alone or for chemoradiation [18,45-47] Assuming that accelerated repopulation...
... high blood pressure For example, subjects with currently normal blood pressure under medication might have had high blood pressure for a long time before it was recognized and treated Therefore, ... A HBP: high blood pressure regardless of its medical treatment - B HBP or medication: high blood pressureor the use of antihypertensive medication - C HBP and medication: high blood pressure ... 0.65 0.83 SBP: systolic blood pressure; DBP: diastolic blood pressure; HBP: high blood pressure (≥140/90 mmHg); FEV1: forced expiratory volume in one second; FVC: forced vital capacity *Medication:...
... used for high- intensity NPPV, preferably in a pressure- limited mode [7, 8, 9] The major target for the ventilatory adjustments (mainly increasing IPAP and respiratory rate) is to achieve normocapnia ... the patient is able to tolerate NPPV for more than two hours, further ventilator adjustments are performed in order to optimise alveolar ventilation according to the results of arterial blood ... Software, Inc., Point Richmond, California, USA) Mean values ± standard deviation were given after testing for normal distribution (Kolmogorov-Smirnov test) For non-normally distributed data, the median...
... is damaged, those organisms can not reproduce themselves and therefore the growth is inhibited However, when UV-damaged DNA is repaired by photoreactivation process, the microorganisms can reproduce ... extraction was performed according to the protocols provided by Qiagen, with a minor modification The extracted DNA was concentrated by using centrifugal filter devices (Centricon; Millipore) and resuspended ... Trihalomethane precursors J Am Water Works Assoc 72: 344-350 10) Oppenheimer, J A., J G Jacangelo, J -M Laine, and J E Hoagland (1997) Testing the equivalency of ultraviolet light and chlorine for disinfection...
... of the coldest spot of the lamp determines the Hg vapor pressure One can therefore use that temperature as a parameter representing Hg vapor density in the discharge The dependence of the 254nm ... installation of the sewage effluent disaffection system for wastewater treatment is shown in Fig The disinfection system is a standard product incorporating a set of electrodeless UV lamps By combining ... for protective quartz sleeves (See fig 3) Structurally, each current path is composed of UV lamps, a submerged weir and an overflow weir to pass the sewage around the lamps uniformly Therefore,...
... Cisco Express Forwarding (CEF) forwarding adjacency entries Before a failure, traffic is being forwarded using both of these forwarding entries On failure of an adjacent link or neighbor, the switch ... EIGRP neighbor information for each access switch and look for the flag indicating queries being suppressed Distribution#sh ip eigrp neighbors detail gig 3/3 IP-EIGRP neighbors for process 100 ... routing or RIP, or a design that leverages a combination of routing protocols (for example, RIP redistributed into OSPF) High Availability Campus Network Design— Routed Access Layer using EIGRP or...
... tetrameric and dimeric forms in Figs and exhibited low orhigh catalytic efficiency Therefore, the various incubated hTK1 forms from Figs and were analyzed for their kinetic behavior with dThd, and ... dithiothreitol), incubated with or without mm of the respective nucleotide or phosphate compound for h at °C, and stored for at least weeks at )80 °C before use for kinetic and molecular mass analyses ... more and more enzyme will be in the high- active tetramer form Recently, phosphorylation of hTK1 at serine 13 has been proposed to be involved in this regulation, preventing ATP-induced transformation...