granuphilin rab27a and syntaxin 1a in mouse pancreatic islets
Substance p chemokine interaction in mouse pancreatic acinar cells, and its implications in acute pancreatitis
... sequences in mammals, include neurokinin A and neurokinin B (Kimura, Goto et al 1984) Tachykinins are produced by three genes, preprotachykinin A (PPTA), preprotachykinin B (PPTB) and preprotachykinin ... domain structure These include an N-terminal domain which is involved in protein-protein interactions, a DNA-binding domain that binds to the consensus sites upstream of its regulated genes and ... IN SUBSTANCE P-INDUCED ACTIVATION OF PKCα/βII, ERK AND JNK IN PANCREATIC ACINAR CELLS 127 5.3.4 PLC AND Ca2+ ARE INVOLVED IN SUBSTANCE P-INDUCED NFκB p65 AND AP-1 c-Jun ACTIVATION IN PANCREATIC...
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Báo cáo khoa học: A novel mass spectrometric approach to the analysis of hormonal peptides in extracts of mouse pancreatic islets ppt
... pancreatic islet extract (Fig 1a) The prominent signals in the spectrum originated from products of proinsulin Two insulin variants are expressed in mouse, insulin and [30] Masses corresponding ... peptides turned out to be those from proinsulin Insulin and and C-peptide and were identified, while the other prominent peaks represent the B-chain of insulin (*) and the truncated form of the C-peptide ... been observed During proinsulin conversion, insulin and C-peptides are produced in equimolar amounts However, in rat pancreatic islets, the C-peptides undergo cleavage, resulting in des-(27–31)-C-peptides...
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Báo cáo Y học: Group IID heparin-binding secretory phospholipase A2 is expressed in human colon carcinoma cells and human mast cells and up-regulated in mouse inflammatory tissues doc
... the fifth painting and subjected to RNA extraction Replicate ear sections were fixed by formalin, embedded in paraffin and stained with hematoxylin and eosin to verify the progress of in ammation ... cationic amino acid residues in their C-terminal regions [21,22] Since the multiple cationic residues in the corresponding C-terminal portions of mouse and human sPLA2-IIAs and rat and human sPLA2-Vs ... (A) Amino acid sequences of the C-terminal part of mouse sPLA2-IID (mIID) and its mutants, KE2 and KE2RS2 Two and four basic amino acids are replaced by glutamic acid or serine in KE2 and KE2RS2,...
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Báo cáo y học: "TWEAK/Fn14 interaction regulates RANTES production, BMP-2-induced differentiation, and RANKL expression in mouse osteoblastic MC3T3-E1 cells" pot
... pathways involved in TWEAKinduced RANTES production in MC3T3-E1 cells, we examined the effects of several inhibitors on TWEAK-induced RANTES production in MC3T3-E1 cells Helenalin (an inhibitor ... inhibited BMP-Smad signaling by inducing serine phosphorylation of the linker region of Smad proteins, resulting in the blockade of Smad nuclear translocation in a certain cell type [22] It is ... using a cytokine protein array (TranSignal Mouse Cytokine Antibody Array 1.0; Panomics, Inc., Fremont, CA, USA) according to the manufacturer's instructions Materials and methods Enzyme-linked immunosorbent...
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báo cáo khoa học: "Dysregulation of miR-15a and miR-214 in human pancreatic cancer" potx
... cultures and clinical samples BxPC-3 human pancreatic cancer cells were maintained in RPMI 1640 medium containing 10% fetal bovine serum (FBS; Gibco BRL) 293T cells were maintained in DMEM containing ... Xiaohua S, Xinyu R, Kai W, Tonghua L: Intrinsic chemoresistance to gemcitabine is associated with constitutive and laminin-induced phosphorylation of FAK in pancreatic cancer cell lines Mol Cancer ... the first-line treatment for advanced pancreatic cancer, and it acts by inhibiting tumor cell proliferation and inducing apoptosis [22-25] Prior to determining the effects of GEM on miR-214, we...
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Identification of oct4 and sox2 targets in mouse embryonic stem cells
... Oct4 and Sox2 levels and binding, and abolishes Stat3 and Smad1 binding 7.10 Knockdown of Oct4 and Sox2 in mESCs differentiates the cells, significantly reduces Oct4 and Sox2 protein levels and ... proteins on biotin-labelled DNA probes containing Oct4 and Sox2 binding sites using EMSA 6.6 Endogenous native Oct4 and Sox2 bind to the composite Sox2-Oct4 joint motif of Ebf1 6.7 Mutations within ... clusters containing maximum overlapping PET (moPET) or more from both Oct4 and Sox2 binding datasets were merged 7.3 Validation of Oct4 and Sox2 overlapping binding sites containing low PET overlaps...
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Tài liệu Báo cáo khoa học: Mouse recombinant protein C variants with enhanced membrane affinity and hyper-anticoagulant activity in mouse plasma pptx
... binding In human proteins, protein S binds to the protein C Gla domain Although this interaction has been mapped to Gla regions C-terminal to the 23 site [31,32], it may still in uence the interaction ... membrane binding affinity enhancement by minor increases in the overall on-rate and ⁄ or minor decreases in the overall off-rate The results obtained in the present study support that the binding of ... results in an increase in the number of binding sites (compare Fig 3D and 3E) and also in an average site with increased affinity If Gla proteins were to bind exclusively to a PS microdomain, then...
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Tài liệu Báo cáo khoa học: Caveolin-1 influences P2X7 receptor expression and localization in mouse lung alveolar epithelial cells docx
... washings in PBG, and a further six washings in NaCl ⁄ Pi, incubation with 2.5% (w ⁄ v) glutaraldehyde in NaCl ⁄ Pi, and further brief washes in destilled water, sections were stained by incubation ... role of caveolae and Caveolin-1 could be to centralize, concentrate and colocalize partner proteins, signaling proteins and effectors within lipid rafts For some proteins, Caveolin-1 may aid 3028 ... localization in subconfluent cells was determined Figure 5A shows a Caveolin-1 staining pattern at the cell surface, confirming the known caveolar location of Caveolin-1 In addition, intracellularly distinguishable...
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Tài liệu Báo cáo Y học: Identification of syntaxin-1A sites of phosphorylation by casein kinase I and casein kinase II ppt
... truncated mutants of syntaxin- 1A (1–190 and 191–265) were subjected to in vitro kinase assays Syntaxin- 1A (1–190), but not syntaxin- 1A (191–265), is a substrate for both kinases indicating that the phosphorylated ... within the N-terminal moiety of syntaxin- 1A (Fig 2) Our data support previous reports indicating that CKII phosphorylates the N-terminal 75 amino acids of syntaxin- 1A while a fragment containing ... confirming the specificity of the kinase activity Phosphorylation of syntaxin- 1A and munc18 by PKA was also investigated Our data in Fig supports previous reports indicating that syntaxin- 1A is...
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Báo cáo khoa học: Regulated expression by PPARa and unique localization of 17b-hydroxysteroid dehydrogenase type 11 protein in mouse intestine and liver pdf
... protein were markedly induced in both the mouse intestine and liver by administration of the PPARa agonist The induction ratios in both tissues were greater than 20, and the induced protein level ... containing Wy-14 643 were prepared and analyzed by western blotting using antibodies to 17b-HSD11 and liver fatty acid-binding protein (L-FABP) [11] 17b-HSD11 was detected only in duodenum and ... Wy14,643 Liver + + Intestine + + 17β-HSD11 CBB-stain Fig Expression of 17b-HSD11 protein in the gastrointestinal tract (A) The gastrointestinal tract of mice [29] fed a diet containing Wy-14 643 for...
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Báo cáo khóa học: Characterization of presenilin complexes from mouse and human brain using Blue Native gel electrophoresis reveals high expression in embryonic brain and minimal change in complex mobility with pathogenic presenilin mutations pptx
... SY5Y and 5-day-old mouse brain were examined using 10% Tris/tricine gels with reducing Ó FEBS 2003 Analysis of mouse and human brain presenilin complex (Eur J Biochem 271) 381 Fig PEN-2 and APH-1 ... 10-kDa band in PS1 wild-type neural stem cells, E15 mouse brain and SY5Y cells Low-level expression was present in PS1 deficient cells, adult mouse brain and in control and AD human brain cortex ... H.T., Tandon, A., Fraser, P.E & St George-Hyslop, P (2003) APH-1 interacts with mature and immature forms of presenilins and nicastrin and may play a role in maturation of presenilin.nicastrin complexes...
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Báo cáo khoa học: Differential expression of liver and kidney proteins in a mouse model for primary hyperoxaluria type I pdf
... Actin, b, cytoplasmic NSFL1 (p97) cofactor (p47) Aminoacylase Phosphoglycerate mutase Indolethylamine N-methyltransferase Peroxiredoxin Apolipoprotein A-I Abhydrolase domain containing 14b D-Amino ... indicate that its alanineglyoxylate aminotransferase activity is not favored over aminobutyrate-pyruvate, b-alanine-pyruvate and dimethylarginine-pyruvate aminotransferase activities In the rat, gluconeogenesis ... in reducing buffer containing 50 mm Tris ⁄ HCl, pH 8.8, 30% glycerol, m urea, 2% SDS and 1% dithiothreitol, followed by a blocking step in similar buffer containing 2.5% iodoacetamide instead...
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Báo cáo khoa học: Lithium increases PGC-1a expression and mitochondrial biogenesis in primary bovine aortic endothelial cells ppt
... neuroprotective in the mutated-hungtintin transgenic mouse model of Huntington disease [30] Interestingly, the preconditioning effects of various substances and factors in brain, heart and vessel ... resulting in increased expression of the cyclin-dependent kinase inhibitor p21cip at both the mRNA and protein levels; this was found to be independent of depletion of the intracellular inositol ... treated as indicated in (A) for min, 30 min, h and 24 h prior to cell harvesting and analysis of GSK3b and Akt phosphorylations using immunoblotting with specific phospho-S9-GSK3b and phospho-S473-Akt...
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Báo cáo khoa học: Regulation of the expression and subcellular localization of the taurine transporter TauT in mouse NIH3T3 fibroblasts doc
... taurine uptake in NIH3T3 mouse fibroblasts is linear within the initial 20 following the addition of 14C-labelled taurine Using taurine uptake within the initial 20 in NaCl medium containing extracellular ... active uptake [7] The data shown in Fig and summarized in Table indicate that the initial taurine uptake is reduced in the presence of b-alanine (Fig 1A) and following substitution of extracellular ... estimated as indicated in Fig in the absence or presence of mM creatine/b-alanine and in Na+-free NMDG-medium, KCl medium or Cl – free NaNO3 medium The absolute taurine in ux (nmolÆg protein)1Æmin)1)...
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Báo cáo Y học: Expression of glucose transporter-2, glucokinase and mitochondrial glycerolphosphate dehydrogenase in pancreatic islets during rat ontogenesis potx
... of hexokinase I and glucokinase protein in rat pancreatic islets and liver Western-blot analyses of glucokinase in pancreatic islets (A) and liver (B) and hexokinase I in pancreatic islets (C) ... GLUT-2 protein in pancreatic islets and livers of fetal, suckling, and adult rats (Fig 2) The intensity of this band was lower in pancreatic islets from fetal and suckling rats than in those from ... different in pancreatic islets and liver Surprisingly, the glucokinase content of pancreatic islets was signi®cantly higher in fetuses than in adult rats and even higher than in suckling animals...
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báo cáo hóa học:" Sigma-2 receptor ligands potentiate conventional chemotherapies and improve survival in models of pancreatic adenocarcinoma" ppt
... Human pancreatic adenocarcinoma cell lines (Panc-1, AsPC-1, and CFPAC-1) were obtained from ATCC (Bethesda, MD) and maintained in Dulbecco's modified eagle's medium (DMEM) containing glutamine ... obtained from Bryan Clary (Duke University) and maintained in supplemented RPMI 1640 containing glutamine (2 mmol/L), pyruvate (1 mmol/L), penicillin (100 IU/ mL), streptomycin (100 IU/mL), and ... split and preincubated for more than 24 hours (Panc-02) and 48 hours (CFPAC-1, AsPC-1, and Panc-1) to maintain their growth conditions SV119 and SW120 were dissolved in DMSO, and gemcitabine and...
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Báo cáo sinh học: "Alpha-1 antitrypsin protein and gene therapies decrease autoimmunity and delay arthritis development in mouse model" potx
... serine proteinase inhibitor (serpin) It inhibits proteinase 3, neutrophil elastase, and cathepsin G These serine proteases are released by joint invading neutrophils following Page 11 of 13 inflammatory ... proteins, involved in proinflammatory cytokine expression and cell signaling [12] It also has been reported that neutrophil elastase inhibitors reduce incidence as well as severity of collagen-induced ... (CIA) in both rats and mice [13] Human AAT is able to completely eliminate the acute inflammatory infiltration and connective tissue breakdown in the lung in a cigarette smokeinduced emphysema mouse...
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Báo cáo sinh học: " Neopterin production and tryptophan degradation during 24-months therapy with interferon beta-1a in multiple sclerosis patients" docx
... 3-nitro-L-tyrosine as an internal standard The coefficient of variation of intra- and interassay determinations for trp and kyn was below 5% Recovery of trp and kyn was determined by measuring trp and kyn in ... IFNb -1a biological activity Initially, IFNb -1a administration may result in a sharp increase in the neopterin levels owing to the acute, proinflammatory actions of IFNb -1a [35,36] However, in the ... Isernia, L’Aquila, Messina, Roma, and Trieste), in collaboration with the University of Innsbruck in Austria and the National Institute of Biological Standards and Control in London, UK The study...
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báo cáo hóa học: " Gait dynamics in mouse models of Parkinson''''s disease and Huntington''''s disease" doc
... dopaminergic system [19], resulting in PD symptoms, including reduced stride length [14] and posture disturbances in mice [20] One common mouse model of HD is obtained by repeatedly administering ... limb of a saline-treated C57BL/6J mouse walking at a speed of 34 cm/s Duration of stride, stance, and swing are indicated for the right hind limb Duration of braking and propulsion are indicated ... gait cycle timing in Parkinson's disease and Huntington's disease Mov Disord 1998, 13:428-437 Blin O, Ferrandez AM, Serratrice G: Quantitative analysis of gait in Parkinson patients: increased variability...
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