... values and standard error of at least two independent experiments are shown (b,c) Summary of replication timing of candidate genes (b) and loci surrounding the Rex1 gene (c) In (c), positions of ... retrotransposons (LINE and SINE B1) and rDNA repeats in wild-type OS25 ES cellsand in mutant ES cells lacking Eed, Dnmt 1, Dnmt 3a/3b, G9a or Dicer The mean values and standard error of at least two ... advanced in Dnmt1 KO and G9a KO ES cells Interestingly, a comparison of matched mutant and wild-type ES cells showed advanced replication of major satellite sequences in the absence of Dicer, consistent...
... NGG5.3 Blm-deficient embryonicstemcellscells were co-cultivated with irradiated B4-5 cellsand plated in normal medium to determine plating efficiency in the absence of selection and 1-(-2-deoxy-2-fluoro-1-β-D-arabino-furanosyl)-5-iodouracil ... (FIAU) to measure the frequency of noninfected cells (b) Assessment of proviral copy number in randomly picked nonselected wild-type and Blm-deficient embryonicstem (ES) cells after co-cultivation ... infected and clones from pools and were highly resistant to infection; clones from pools 2, 3, and were less resistant to infection mutant clones from pools 1, 7, 2, and were termed V5, V4, V3, V2 and...
... PUBLICATIONS LIST OF ABBREVIATIONS CHAPTER I INTRODUCTION 1.1 Pluripotent stemcells 1.1.1 Mouse embryonic carcinoma cells 1.1.2 Mouse embryonicstemcells 1.1.3 Mouse embryonic germ cellsand pluripotent ... spermatogonial stemcells or adult germline stemcells have been derived from spermatogonial cellsof adult mouse and human testis These cells are able to differentiate into various types of specialized cells ... potentials: totipotent stem cells, pluripotent stem cells, multipotent stem cells, and unipotent stemcells A totipotent cell can develop into a whole organism Pluripotent cells can give rise to...
... further analysis Refinement and increased efficiency of these two ESC protocols brings the potential to offer a reliable supply ofembryonicstemcells without production of whole embryos or compromising ... 1981 Landry DW, Zucker HA: Embryonic death and the creation of human embryonicstemcells J Clin Invest 2004, 114:1184-1186 Voullaire L, Slater H, Williamson R, Wilton L: Chromosome analysis of ... be derived from subsequent isolation and re-plating of the ICM cells, which are then screened for stemness by a variety of recognized markers Once in stable culture, ESCs are capable of either...
... and cartilage degeneration after a temporary relief of symptoms Stemcells represent a promising cell source for cartilage repair and can be derived from two major sources: mesenchymal stemcells ... potential of hESCs for cartilage regeneration and tissue engineering 2.2.1 Expansion of hESCs Human embryonicstemcells are conventionally co-cultured on mouse embryonic fibroblast (MEF) feeder cells ... tissue engineering (Chung et al., 2008) Human embryonicstemcells (hESCs) are stem cell lines ofembryonic origin, isolated from the inner cell mass of the blastocyst-stage embryo (Thomson et al.,...
... and demand sides of broadband and next generation deployment This document is accompanied by a series of select country overviews, in which we offer countryspecific overviews of performance and ... Percentage of households with broadband access Source: Berkman Center analysis of Eurostat data The National Broadband Task Force has provided a broad review of the uses and benefits of broadband, from ... Generation Connectivity The only substantial change is that Sweden moves from 7th to 1st place, nudging Denmark and the Netherlands from first and second to second and third places, and Finland...
... erythroid differentiation of murine embryonicstemcells PCR analysis of DNase I treated and reverse-transcribed total RNA extracted from differentiating embryonicstemcells at the indicated time ... ES cellsand in human hematopoietic progenitor cells Discussion B Fig Transcription of LCR hypersensitive site in undifferentiated murine embryonicstemcellsand in human CD 133+ bone marrow cells ... the developmental progression of cell samples and in all cases except that of the Rex-1 gene, primer sets span introns Day cells are composed of ES cellsand MEF cells grown in ES media containing...
... expanded cells as a mixture of ECs and MCs derived from human ES cells (hESECs+MCs) The cell mixture was composed of almost the same number of ECs and MCs We resorted the VE-cadherin+ cellsfrom ... VEGF-R2-positive cells induced from undifferentiated mouse embryonicstem (ES) cells can differentiate into both VE-cadherin-positive endothelial cells (ECs) and αSMA-positive mural cells (MCs), and these ... Characterization of the transplanted vascular cells derived from human ES cells (HES-3) Characterization of the transplanted vascular cells derived from human ES cells (HES-3) A, Flow cytometric analysis of...
... profiles of miRNAs and transcripts using three hES cell lines, embryonic bodies (EB) produced from one of the cell lines and five types of terminally differentiated adult cells The integration of miRNA ... either EB or adult cells (Figure 6, panel A) The difference in the expression of miR-200c, miR-302b, and miR-367 between hES cellsand EB, and between hES cellsand adult cells was significant ... in adult cells (Figure 6, panel C) The differences in expression of these miRNA expression between adult cellsand hES cellsand between adult cellsand EB were significant (P < 0.05) Of note,...
... bottom and the top of the felled trees in order to estimate stem taper and to verify the cylindrical stem assumption Wood discs were sampled at each end of the stem immediately after the felling of ... large board MOE and the mean of the two top standard samples There is a good relationship between the large board MOE and both means of the two halfues boards andof the four standard samples ... m of the stemof standing Douglas fir, i.e of the most valuable part of these trees The strongest relationship between the trunk MOE and a destructive sample MOE is obtained with the mean of...
... culture and cell surface epitope characterisation of IPFP cells The cells isolated from the IPFP proliferated in culture and reached confluence by day 14 The yield ofcells by the end of passage ... culture systems it has been shown that under hypoxia there is increased synthesis of extracellular matrix by chondrocytes [15,16], and this has been extended to stemcellsfrom bone marrow [17] and ... differentiation and matrix production of chondrocytes, but few studies have characterised gene expression changes In our investigation of the potential of IPFP-derived stemcellsfrom elderly patients...
... embryonicstem cell; hESC, human embryonicstem cell; H3K4me3, trimethylation of lysine of histone H3; H3K27ac, acetylation of lysine 27 of histone H3; H3K27me3, trimethylation of lysine 27 of ... pluripotent embryonicstemcellsand differentiation Nat Rev Mol Cell Biol 2006, 7:540-546 51 Park SH, Kook MC, Kim EY, Park S, Lim JH: Ultrastructure of human embryonicstemcellsand spontaneous and ... Induction of pluripotent stemcellsfrom mouse embryonicand adult fibroblast cultures by defined factors Cell 2006, 126:663-676 Yamanaka S: Strategies and new developments in the generationof patient-specific...
... of osteogenic cells derived from human embryonicstemcells Tissue Eng 2004, 10:1518-1525 17 Chaudhry GR, Yao D, Smith A, Hussain A: Osteogenic Cells Derived FromEmbryonicStemCells Produced ... the differentiation ofstemcells into the osteogenic lineage in vitro J Bone Miner Res 2004, 19:1379-1394 Page of 38 Trounson A: Human embryonicstem cells: mother of all cell and tissue types ... differentiation of transplantable neural precursors from human embryonicstemcells Nat Biotechnol 2001, 19:1129-1133 doi:10.1186/1746-160X-7-12 Cite this article as: Handschel et al.: Embryonicstem cells...
... Preparation of conditioned media from !E-MEFs 33! 3.2.3! Human embryonicstemcellsand induced pluripotent stemcells 33! 3.2.4! Embryoid body formation 33! 3.2.5! Generationof stable ... specification of the neuroectoderm (Muñoz-Sanjuán and Brivanlou, 2002) Bmp are secreted from roof plate cells in the dorsal neural tube and induce formation of neural crest stemcellsand dorsal ... vitro system to study complex events underlying human nervous system development (Ben-Nun and Benvenisty, 2006; Dvash et al., 2006) 2.7 Embryonicstemcellsand induced pluripotent stemcells Embryonic...
... haematopoietic stemcells that give rise to blood cells (Moore and Lemischka, 2006; Petersen and Terada, 2001) (Figure 1.2) Stem cells, particularly embryonicstemcells (ESCs), offer enormous ... differentiation of mESCs reduces Oct4 and Sox2 levels and binding, and abolishes Stat3 and Smad1 binding 7.10 Knockdown of Oct4 and Sox2 in mESCs differentiates the cells, significantly reduces Oct4 and ... give rise to the germ cell and somatic lineages of the embryo (D) Figure was adopted from Niwa (2007) 1.2 Embryonicstemcells (ESCs) Cellsfrom the ICM can be isolated and cultured in vitro under...
... classes of pluripotent stemcells in humans, other primates and mice These are embryonicstem cells, embryonic germ cells, embryonic carcinoma cellsand recently the discovery of a fourth class of ... somatic stemcells can be found in diverse tissues and organs The most common adult stemcells are the hematopoietic stem cells, mesechymal stemcellsand neuronal stemcells Adult stemcells have ... blood cellsand platelets, while skin stemcells give rise to the various types of skin cells Some recent reports suggest that adult stemcells such as haemopoietic stem cells, neuronal stem cells...
... the mouse vii LIST OF FIGURES Figure 1.1 Embryonic origin of human embryonicstemcellsand their in vitro characterisation Figure 1.2 Lineage tree of embryo-derived cellsand cell lines Figure ... Lineage tree of embryo-derived cellsand cell lines The various stages ofembryonic development from fertilization to E6.5 are represented in this image EmbryonicStem (ES) cells are derived from the ... CD34+/ c-Kit+ cells isolated from the yolk sac and separately from the P-Sp at the same stage of development showed presence of long-term repopulating stemcells (LTRSCs) LTRSCs are stemcells that...
... Introduction 1.1 Human embryonicstemcells 1.1.1 Overview and characteristics of human embryonicstemcellsEmbryonicstem (ES) cells are isolated from the inner cell mass (ICM) of embryos of blastocyst ... Human EmbryonicStemCells Undifferentiated HES4 Human EmbryonicStemCells SAGE _Embryonic_ stem_ cell_H9_normal_p38_CL_SHES1 LSAGE _Embryonic_ stem_ cell_BG01_normal_p20_CL_SHE19 LSAGE _Embryonic_ stem_ cell_H13_normal_p22_CL_SHE15 ... List of Figures VIII List of Abbreviations IX Introduction 1.1 1.2 Human embryonicstemcells 1.1.1 Overview and characteristics of human embryonicstemcells 1.1.2 Regulatory networks and transcription...
... Technology Institute (BTI) and the Agency for Science Technology and Research (A*STAR) Thanks to Prof Wang Nai-Dy, Prof Miranda Yap, Prof Tan Bor Leung, Prof James Chen, and Dr Paul Robson for their ... Knockdown of Oct-3/4 and Sox-2 Transcription Factors in Mouse EmbryonicStemCells a Establishment of Efficient Transfection Method b Oct-3/4, Sox-2 Knockdown and Differentiation Into Trophectoderm Generation ... posttransfection and five days of selection were sufficient to achieve complete elimination of GFP-C2 negative control cells Mouse embryonicstemcells transfected with pcDNA6/TR and surviving Blasticidin...
... VS, Jones JM Embryonicstem cell lines derived from human blastocysts Science 1998;282(5391):1145-7 Reubinoff BE, Pera MF, Fong CY, Trounson A, Bongso A Embryonicstem cell lines from human blastocysts: ... M, Fong CY, Tan S, Chan WK, Bongso A An efficient and safe xeno-free cryopreservation method for the storage of human embryonicstemcellsStemCells 2004;22(5):779-89 10 Speicher MR, Ballard SG, ... establishment of new hESC lines emphasized the use of temperature-heated stage and pre-warmed and pre-equilibrated culture media Nevertheless, the results of this study would prove otherwise hESC of the...