... is the characteristics of this situation type that influence the forms of language that realize the genre So the context of situation (register) is the second aspect of social context that influences ... into the role of metaphor in description of emotion in poetic discourse Levels of Language While SFL accounts for the syntactic structure of language, it places the function of language as central ... he was careless, a traffic accident occurred [2b] His carelessness caused a traffic accident Taking condition as process To express the meaning of condition in the congruent way, connectives...
... learning how to it themselves The third cause is lack of access to computer The fourth cause is dissatisfactory school base The fifth cause is insufficient allocation of application programs, - ... alternative of solving the problem, what can we suggest? We have another alternative, which is called “Simplification of access to computer classes.” We mean the organization of computer classes or computer ... which can be solved with the help of PC faster And all we know that computer literacy is on ofthe main requirement at employment Thus we see that the problem of computer illiteracy is oneof the...
... eukaryotic c class cytochromes [27]; anions exert a strong influence on the lysine residues of cytochrome c, and significantly affect the structure and the functional properties ofthe protein, as the conserved ... modulates the structure ofthe A state of cytochrome c Biochemistry 39, 12632– 12638 18 Sinibaldi F, Howes BD, Smulevich G, Ciaccio C, Coletta M & Santucci R (2003) Anion concentration modulates conformation ... Fig Fig Absorbance at 695 nm of acid-denatured cytochrome c in the presence of increasing sulfate (s) and selenate (d) concentrations The optical absorbance of native cytochrome c (—) at pH 7.0...
... sequence in chitosan is of four types, -GlcN-GlcN-, -GlcN-GlcNAc-, -GlcNAc-GlcNand -GlcNAc-GlcNAc-, of which the first is the major type and the last one results from the heterogeneous de-Nacetylation ... nonreducing ends, i.e -GlcNAc-GlcNAc-linkage, the action on which results in the release of GlcNAc and products with GlcNAc at the reducing ends The observed decrease in DA value of LMWC is due to the ... difference in chemical shift values of C1 and C4 indicated a conformational change ofthe glycosidic linkage in LMWC Multiplicity ofthe peak corresponding to C4 is independent of DA and is associated...
... The torque was created by external forces acting on the two groups of four carbon atoms each The first group included the Ca atoms of cK18, cI19, cT20 and cK21, and the second group Ca atoms of ... from the N-end ofc subunit (cA1–cK24) and 43 residues from its C- end (cT230– cL272) The chosen portion included a major part ofthe coiled-coil region ofc and the complete a-helical C- terminus ... distance of 1.2 nm The system was equilibrated during ns, and then the rotation ofc was forced by a constant torque applied to the coiled-coil portion ofc at the level of cK18–cK21 and cD233–cS236...
... that of genes CYC1 and CYC7, which encode iso-1 and iso-2 of yeast cytochrome c, respectively CytOX 5a and CYC1 are coexpressed under aerobic conditions (O2 > 0.5 lM), whereas CytOX 5b and CYC7 ... nonspeci c and toxic effects in animals To ascertain that the effects of these agents, on the catalytic activity and subunit composition of CytOX were related to their hypoxia-speci c effects, we ... also the catalytic activity ofthe enzyme by affecting its stability or composition Although succinylacetone and CoCl2 are known inhibitors of heme biosynthesis, these agents also elicit nonspecific...
... study The lectin is unique from that of other sialic acidspeci c lectins O-Acetyl sialic acid-speci c lectin was Ó FEBS 2003 A sialic acid speci c lectin from P jacquemontii (Eur J Biochem 270) ... fractions collected on ice in polypropylene tubes containing 100 lL of 100 mM CaCl2 at a rate of 0.3 mLÆmin)1 The presence of calcium chloride was required in the collected fractions because the ... erythrocytes was marked by a 9-O-acetyl sialic acid-speci c lectin purified from the hemolymph ofthe snail Achatina fulica [64,66] Lectins are used for verification ofthe sugar specificity of the...
... and (c) inactivation of EIIGlc is accelerated in the presence of Glc (see below) Although the dominant reactivity of Cys421 compromised the labelling of other active-site residues, the glucose ... II-BGlc, a glucose receptor ofthe bacterial phosphotransferase system: molecular cloning of ptsG and purification ofthe receptor from an overproducing strain of Escherichia coli Proc Natl Acad Sci ... biphasic to a monophasic shape are Table Rates of inactivation of IICBGlc Incubation of purified IICBGlc with the indicated concentration (mM) ofthe analogues 1a)3d was carried out at 30 C Rate constants...
... from the cDNA #911 using the following pair of primers: 5¢-CTGGATCCCT ATGTCGTGTCCCAGGAGCATCGAG-3¢ and 5¢-GTC TGCAGTTAAAAATTCGGGACATTCCTTAGCCA GG-3¢ BamHI–PstI digested PCR product was cloned ... following pair of primers: 5¢-CAGAATTCA TGACACTTCCTAGTGCGGCTCGC-3¢ and 5¢-CTG GATCCTTATTGCTGATTATTGGGATTCATTTGA CCA-3¢ EcoRI–BamHI digested PCR product was cloned as a fusion with the GAL4 activation ... primers: 5¢-CAGGATCCCTATGAGCAGC TCCGAGGAAGTCTCCT-3¢ and 5¢-CTGTCGACTTA GTTTTTCGCTCGTAGTGGCATTTTAAAATTGGCT GC-3¢ BamHI–SalI digested PCR fragment was cloned into BamHI–SalI digested pAS2-1 vector, or...
... indicating that proteolytic modifications ofthe D-loop affect the state ofthe interdomain cleft [5,17] The D-loop of actin can be specifically cleaved with two bacterial proteases Oneof them ... the open cleft conformation The cleavage within the D-loop enhances the nucleotide exchange [17] and increases accessibility ofthe cleft to limited proteolysis [5], which characterizes the cleft ... stabilizing the filament by closing the cleft in actin subunits [49,50] Hence, the increase in the thermal stability of ECP-cleaved F-actin and the disappearance ofthe shoulder on the DSC profile can...
... ‘nested PCR’, using the first PCR product as template To make use ofthe BamHI site in the vector and the XbaI site in the EhMGL1 gene, the product ofthe nested PCR was replaced with the corresponding ... vivo activity ofthe two isozymes in the parasite, we measured speci c activities of MGL in the amoebic extracts using two representative physiological substrates, i.e Met and Hcy The speci c activities ... toxicity to the cell The fact that EhMGL2, which is more active in the degradation of TFM, is less sensitive than EhMGL1 seems to contradict the notion that the product ofthe degradation is the enzyme...
... 5¢-CACGATGATGGATGAAATGGCG TC-3¢ For NKA49: forward, 5¢-CTTCCACGACGGAAAC GATGAC-3¢; reverse, 5¢-CTCTCCAACACATGCTGACG TAG-3¢ Cycling conditions were 94 C for min, followed by 35 cycles of 94 C for 30 s, 54 C for ... 10 lm of each ofthe following primers For NKA8: forward, 5¢-GTCTCCAGACGTCTCGAAC-3¢; reverse, 5¢-CATCCAAGTCTTGGGAGCTC-3¢ For NKA48: forward, 5¢-GATGCTAACTTCAGCGGAAAC TC-3¢; reverse, 5¢-CACGATGATGGATGAAATGGCG ... first-strand cDNA and the following primers (NKA48, accession number DQ017261): 5¢-GATGCATAATACGACTCACTATAGG GAAATGTCCGTCCAACAAGGAG-3¢ (forward) and 5¢GCCTTCTAATACGACTCACTATAGGGACCACGATG ATGGATGAAATG-3¢...
... noticed earlier [36,37] Figure shows the effect ofthe FMN concentration on the reconstitution ofthe activity ofthe reduced SH Addition of about 80 nM FMN induced half maximal activity Table The ... NADHK3Fe(CN)6 activity is the speci c activity compared to that of untreated enzyme Bound, acid-labile FMN from the protein inside the dialysis bag, corrected for the contribution ofthe free FMN in the ... Scienti c Research (NWO), the Deutsche Forschungsgemeinschaft, the Fonds der Chemischen Industrie, EU-project BIO4-98-0280 and the European Union Cooperation in the field of Scienti c and Technical...
... the third encoded methionine [9], the sequence ofthe human B1wt, truncations and chimeras of both were cloned into the BamHI and the XhoI sites ofthe pcDNA5 ⁄ FRT vector from Invitrogen Each ... accumulation of total IPs for 30 at 37 C compared to the IP content of control cells that had remained at C There was a clear correlation between the agonist-inducible internalization and the ... recognition site because the presence of detergent or membrane lipids influences the formation of a helical structure These authors proposed that activation ofthe receptor, and subsequently of...
... with a loved one can evaporate because ofthe way the chemicals in those substances affect the brain Your immediate reality can change in an instant If chemicals acting on the brain can create different ... together the findings of innumerable articles and books, both technical and popular, along with accounts of patients she treated at her clinic Given the character— and rancor of our dichotomous ... to touch a toy cow The mother stood off to the side Every move, glance, and utterance was recorded Very few ofthe girls touched the forbidden object, even though their mothers never explicitly...
... is considered acceptable The total cost of food imported by the neediest countries rose 25 percent in 2007.7 Some Blame African Hunger on Rejection of Agricultural Biotechnology According to the ... population At the same time, the FAO calls for a cautious, case-by-case approach to determine the benefits and risks of each individual biotech crop genetic event and to address the “legitimate concerns ... export-led economic growth.13 Increased Production and Plantings Since the first commercialized crop in 1996, the world’s farmers have consistently increased their plantings of biotech crops by...
... according to established protocols [50] Experimental protocols were reviewed by the Animal Care Committee of Dalhousie University in accordance with the recommendations ofthe Canadian Council ... (2001) Cloning and sequence ofthe gene encoding the muscle fatty acid binding protein from the desert locust, Schistocerca gregaria Insect Biochem Mol Biol 31, 553–562 18 Ceciliani F, Monaco HL, ... utilization of fatty acids, intracellular targeting of fatty acids to speci c organelles and metabolic pathways, and the protection of cellular structures from the detergent effects of fatty acids [10–14]...