extraction rt pcr and sequencing
Báo cáo y học: " Measurement of Epstein-Barr virus DNA load using a novel quantification standard containing two EBV DNA targets and SYBR Green I dye" pps
... cost-effective and just as sensitive as the widely used TaqMan PCR [21,28-30] Here, in an effort to ascertain the relationship between EBV DNA load and disease, two real-time quantitative PCR (QPCR) assays ... control for PCR inhibitors and check for DNA integrity [35] All samples were subjected to beta-globin PCR prior to EBV QPCR Contamination was monitored by the use of PCR- grade water and no template ... necessity for two separate EBV standards, thus reducing costs and labour The EBNA-1 and BHRF-1 DNA targets were linked using randomised primers (Table 1) and inserted into the pGEM vector, using...
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Sputum smear negative pulmonary tuberculosis: sensitivity and specificity of diagnostic algorithm docx
... supported by the Muhimbili University of Health and allied Sciences We wish to thank Dr Kazema and Dr Kimaro (X-ray and imaging specialists) who read all the X-rays We thank and Mr Ngowi and Mr ... smear negative and AFB sputum If HIV Positive Clinical assessment Request CXR Give CPT Visit Treat for TB and give CPT if HIV + Visit TB likely: treat for TB and give CPT CXR suggestive and clinical ... chest X-ray done, and all of them were reviewed and reported using a structured format by two independent radiologists In case of disagreement in their initial independent reporting, they reviewed...
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Báo cáo sinh học: "An automated stochastic approach to the identification of the protein specificity determinants and functional subfamilies" pot
... preparation AAM and ARR participated in the design of the algorithms ABR and RBR participated in the data collection OVK participated in the design of the algorithms, data collection, and analysis and preparation ... contributions PVM participated in the design of the algorithms and data analysis, performed the programming and drafted the manuscript MSG participated in the data collection and analysis and manuscript ... [20], and reflects proximity of two distributions, in our case, the gold standard and the predicted grouping of sequences In the case of generated families we have the standard of truth given artificially:...
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Báo cáo khoa học: " Detection and identification by PCR of Clostridium chauvoei in clinical isolates, bovine faeces and substrates from biogas plant" potx
... originated from C chauvoei, further analysis was performed by 16S rRNA gene sequencing The PCR product http://www.actavetscand.com/content/51/1/8 was diluted and cycle sequencing reactions were carried ... chauvoei were analysed by culture and biochemical identification, culture and PCR and swab samples from the same muscle samples were analysed by culture and PCR The swabs were used to simulate ... 1996, 44:175-178 Burtscher C, Wuertz S: Evaluation of the use of PCR and reverse transcriptase PCR for detection of pathogenic bacteria in biosolids from anaerobic digestor and aerobic composters...
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Detection and identification of mean shifts in multivariate autocorrelated processes a comparative study
... the Shewhart chart, EWMA chart, SCC chart and CCC chart when they are used to control ARMA(1,1) processes They show that SCC and CCC charts perform better when the shift size exceeds standard deviations ... chart based on the residuals with the EWMA chart based on the original observations and the Shewhart chart Results show that the EWMA chart based on the residuals is comparable to the EWMA chart ... of shift in X and Y, various levels of autocorrelation of X and Y, and correlation between X and Y should be investigated The shift sizes in X and Y are set to 0, 0.5, 1, and Further, the shift...
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Báo cáo y học: "Identification of clinical and simple laboratory variables predicting responsible gastrointestinal lesions in patients with iron deficiency anemia"
... therefore crucial and may be of far greater importance to the ultimate well-being of the patient than repleting iron stores This is particularly important, because a large proportion of patients ... endoscopic and radiographic Asymptomatic colonic and gastric carcinoma may present with IDA and exclusion of these conditions is of prime concern The upper endoscopic evaluation should include random ... criteria and were enrolled Their mean age was 43.3 (19-81) years 71 were patient aged under 50 and 20 were over 50 years 77 were female and 14 were male Sixty-six of women were pre-menopausal and...
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Detection of bacteria and enteric viruses from river and estuarine sediment
... collected in the Takagi River partly because of high proportion of fine particles such as silt and clay and presence of humic substances Direct DNA extraction method using DNA extraction kit for soil ... Tsai and Olson (1992), Zhou et al (1996) and Watson and Blackwell (2000) reported The color of supernatant after vortex mixing and centrifugation was brown and that of the concentrate after membrane ... Tokyo) Detection of pathogens and fecal indicators by PCR and qPCR methods The concentration of (c)DNA of Poliovirus and Adenovirus was determined using real-time qPCR methods with LightCycler...
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Analytical study to minimize the engine exhaust emissions and safe knock limit of CNG powered four-stroke SI engine
... pressure and temperature are Pu = 0.1 MPa, and Tu = 300K, respectively ul (cm/s) is the burning velocity at Pu and Tu αT And βT as well as ul , are functions of equivalence ratio ( ∅ ), and are ... band pass filter, third derivative and step method It was also reported that the vibration signal is more useful among these methods Heywood [1], Chun et al [15], Moses et al [16], Checkel and ... ratio, and engine knock occurs more easily if the compression ratio is increased In order to fully understand the knock phenomenon and to find possible way to avoid knock phenomenon, Moses [16], and...
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Tài liệu The Practical Guide: Identification, Evaluation, and Treatment of Overweight and Obesity in Adults pdf
... and Obesity in Adults is largely based on the evidence report prepared by the Expert Panel and describes how health care practitioners can provide their patients with the direction and support ... Heart, Lung, and Blood Institute National Institutes of Health Van S Hubbard, M.D., Ph.D National Institute of Diabetes and Digestive and Kidney Diseases James P Kiley, Ph.D.* National Heart, ... Guidelines on the Identification, Evaluation, and Treatment of Overweight and Obesity in Adults: Evidence Report was released by the National Heart, Lung, and Blood Institute’s (NHLBI) Obesity Education...
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Tài liệu Báo cáo khoa học: "Automatic Extraction of Lexico-Syntactic Patterns for Detection of Negation and Speculation Scopes" pdf
... resulting subtree were converted to a generalized representation: scope words were converted to *scope*; noncue and non-scope words were converted to *; cue words were converted to lower case Figure ... report 61.13 and 79.89 accuracy for BioScope full papers and abstracts respectively Similarly, Morante and Daelemans (2009b) developed a machine learning system for identifying hedging cues and ... reported as F1-scores of 38.16, 59.66, 78.54 and for clinical texts, full papers, and abstracts respectively3 Accuracy (computed for correctly identified scopes) was reported as 26.21, 35.92, and...
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Tài liệu Báo cáo khoa học: "Automatic Identification of Pro and Con Reasons in Online Reviews" ppt
... pro and candidate sentences (CR and PR in Table 1) from sentences irrelevant to either of them (NR) The classification task then classifies candidates into pros (PR) and cons (CR) Section reports ... Cardie, C., Riloff, E., and Patwardhan, S 2005 Identifying Sources of Opinions with Conditional Random Fields and Extraction Patterns Proceedings of HLT/EMNLP-05 Esuli, Andrea and Fabrizio Sebastiani ... Hatzivassiloglou, and Dan Jurafsky 2004 Automatic Extraction of Opinion Propositions and their Holders, AAAI Spring Symposium on Exploring Attitude and Affect in Text: Theories and Applications...
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Tài liệu Obesity guidance on the prevention, identification, assessment and management of overweight and obesity in adults and children ppt
... to: • senior managers and budget holders in local authorities and community partnerships, who manage, plan and commission services such as transport, sports and leisure and open spaces (not just ... drinks and confectionery high in added sugars other food and drinks high in fat and sugar, such as some takeaway and fast foods Eat breakfast • Watch the portion size of meals and snacks, and how ... play time, and provide regular opportunities for enjoyable active play and structured physical activity sessions − implement Department for Education and Skills, Food Standards Agency and Caroline...
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Perspectives of Chief Ethics and Compliance Officers on the Detection and Prevention of Corporate Misdeeds ppt
... corporate accounting and fraud scandals by mandating new ethics hotlines, codes of conduct, and stronger internal controls and reporting efforts, but here again, these steps are only part of the overall ... to integrity and corporate ethics starts with a seniorlevel chief ethics and compliance officer (CECO) who understands the compliance and ethics field, is empowered and experienced, and who has ... and Compliance Officer An effective approach to integrity and corporate ethics starts with a senior-level CECO who understands the compliance and ethics field, is empowered and experienced, and...
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INTERNATIONAL STANDARD Microbiology of food and animal feeding stuffs — Horizontal method for the detection of Salmonella spp docx
... other than milk and milk products, meat and meat products, and fish and fishery products [4] ISO/TR 11133-1, Microbiology of food and animal feeding stuffs — Guidelines on preparation and production ... followed and the reasons for deviations from this method in the case of particular products The harmonization of test methods cannot be immediate, and for certain groups of products International Standards ... representative and has not been damaged or changed during transport or storage Sampling is not part of the method specified in this International Standard See the specific International Standard dealing...
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Báo cáo khoa học: "Automatic Detection and Correction of Errors in Dependency Treebanks" potx
... the one hand the fact that some of the phenomena are too rare and are not captured by their models On the other hand, in many other cases parsers make correct predictions, but the gold standard ... strings and briefly compare the gold standard dependency annotation of a certain dependency within these sentences Together, the two stocks wreaked havoc among takeover stock traders, and caused ... importance for the community Nevertheless, errors can be found even in the most popular and widely-used 349 resources In this paper we have presented an approach for automatic detection and correction...
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Báo cáo khoa học: "Detection of Agreement and Disagreement in Broadcast Conversations" pot
... discussions 375 or calling the meeting Reporting participant is a person reporting from the field, from a subcommittee, etc Commentator participant/Topic participant is a person providing commentary ... markers and noise, removing punctuation and case information, and conducting text normalization We also built automatic rule-based and statistical annotation tools for these LUCs Features and Model ... and energy, we 376 calculated the minimum, maximum, range, mean, standard deviation, skewness and kurtosis values A decision tree model was used to compute posteriors from prosodic features and...
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Báo cáo khoa học: "Semantic Types of Some Generic Relation Arguments: Detection and Evaluation" pptx
... positive and negative examples, one first builds the maximal canonical automaton (MCA) (Pernot et al., 2005) with one starting state and a separate sequence of states for each positive example and ... subsumer (LCS) of the nodes Given the bipartite graph in Figure 1, it can be done as follows For every vertex Gxi in one part which is connected to several vertices Gy1 , , Gyk in the other, we ... material and Y is the entity derived from the origin Theme-Tool(X,Y) The tool Y is intended for X is either its result or something that is acted upon Part-Whole(X,Y) X is part of Y and this...
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Báo cáo khoa học: Detection and characterization of a novel extracellular fungal enzyme that catalyzes the specific and hydrolytic cleavage of lignin guaiacylglycerol b-aryl ether linkages pdf
... solution and centrifuged at 4000 g for 15 Half of the residue was designated the hyphae fraction (HP) and the remainder was homogenized with mortar and pestle for in liquid nitrogen and suspended ... aO and 0.2% conyferyl alcohol, and 20 mL of 3% H2O2 were dropped into 430 mL of 100 mM potassium phosphate [pH 6.0, containing 30% acetone and mg horseradish peroxidase (44 UÆmg)1, Sigma)] and ... mg of peroxidase was added and the reaction mixture was stirred for additional 12 h The resultant precipitate was collected and washed three times with 10 mL water and centrifuged at 4000 g for...
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Detection of actual and assessment of potential plantations in Lao PDR using GIS and remote sensing technologies doc
... rivers and lakes: Within the landscape rivers and lakes have two different functions On one hand they are natural barriers separating landscapes, and on the other hand they can serve as transport ... northwards of Luang Namtha and the land cover difference between Laos (on the left) and China (source: Google Earth, accessed in July 2008) Nevertheless, large areas have already been converted ... Land cover The land cover data was provided by the Ministry of Agriculture and Forestry (MAF) and represents the land cover in 2002 The dataset is part of a forest cover and land use changes...
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low - level detection of ethanol and h2s with temperature - modulated wo3 nanoparticle gas sensors
... being 0.3 mm apart and mm in length and having a Pt heating resistor on the reverse side [31] The nanoparticle WO3 film was prepared using an advanced gas deposition unit (Ultra Fine Particle Equipment, ... The gas flow transported the tungsten oxide vapour upwards and as the vapour became cooler, first oxide molecular clusters and then WO3 nanoparticles were formed by condensation Part of the gas flow ... wavelets was used because of its desirable properties of orthogonality, approximation quality, redundancy, and numerical stability [33]; in particular, the fourth order Daubechies is convenient as it...
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Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam xác định các mục tiêu của chương trình xác định các nguyên tắc biên soạn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản khảo sát chương trình đào tạo gắn với các giáo trình cụ thể xác định thời lượng học về mặt lí thuyết và thực tế điều tra đối với đối tượng giảng viên và đối tượng quản lí khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam nội dung cụ thể cho từng kĩ năng ở từng cấp độ mở máy động cơ lồng sóc mở máy động cơ rôto dây quấn các đặc tính của động cơ điện không đồng bộ đặc tuyến hiệu suất h fi p2 đặc tuyến tốc độ rôto n fi p2 đặc tuyến dòng điện stato i1 fi p2 động cơ điện không đồng bộ một pha sự cần thiết phải đầu tư xây dựng nhà máy thông tin liên lạc và các dịch vụ phần 3 giới thiệu nguyên liệu chỉ tiêu chất lượng 9 tr 25