extraction microarray hybridization and scanning
Báo cáo y học: "Structure and dynamics of the pan-genome of Streptococcus pneumoniae and closely related species" docx
... PcsB and StkP [41] The distribution and level of conservation of each of these proteins within pneumococcal strains and the presence in S mitis, S oralis and S infantis are reported in Table and ... event and the ratio θr/θ, where θ is the mutation rate θr was estimated using the formula θr = θ·r·l, where l is the average length of the recombining segments, and θ and r are the mutation and ... genes and the number of core, dispensable (that is, missing in at least one strain), and strain-specific genes for S pneumoniae alone, for S pneumoniae, S mitis, S oralis, and S infantis, and for...
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Báo cáo khoa học: Identification of yeast aspartyl aminopeptidase gene by purifying and characterizing its product from yeast cells potx
... fractionation, and chromatography Mono Q chromatography was used to separate aspartyl aminopeptidase activity into three major peaks (Fig 1) The first and second peaks were pooled as fraction I and II, ... activity in Mono Q fractions I and II using a Superose column (s, d) Activity of fractions I and II, respectively (B, C) Results of SDS ⁄ PAGE analysis of fractions I and II Lane numbers correspond ... bacteria and eukaryotes [6] We selected 12 of these and aligned their sequences using the clustal_x program [7] (Table 3) This revealed three conserved His residues, two conserved Glu residues, and...
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Báo cáo khoa học: Oxygen control of nif gene expression in Klebsiella pneumoniae depends on NifL reduction at the cytoplasmic membrane by electrons derived from the reduced quinone pool doc
... same medium but lacking sulfide and resazurin and with N2 as the gas phase Main cultures (25 mL) were inoculated from precultures and incubated under a N2 atmosphere and strictly anaerobic conditions ... bands were detected with secondary antibodies directed against rabbit IgG and coupled to horseradish peroxidase (Bio-Rad Laboratories) Purified NifA and NifL from K pneumoniae were used as standards ... Center, personal communication) and using the database, ERGO (Integrated Genomics, Inc.; http://www.integratedgenomics.com) Materials and methods The bacterial strains and plasmids used in this study...
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Báo cáo y học: "Reduction of urate crystal-induced inflammation by root extracts from traditional oriental medicinal plants: elevation of prostaglandin D2 levels" pps
... PGE2 and PGD2 levels were determined by commercially available immunoassays (eBioscience, San Diego, CA, USA (IL-6) and Cayman Chemical, Ann Arbor, MI, USA (PGE2 and PGD2)) RNA extraction and ... [9] and reduced expression of proinflammatory factors in experimental colitis in rats [10] The flavonoids baicalein, which binds to chemokine ligands and inhibits leukotriene C4 synthesis, and ... pouch wall and fluid and reducing the synthesis of pro-inflammatory factors Increase in the level of prostaglandin D2 by treatment with the root extracts PGD2 is a pleiotropic prostaglandin that...
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Báo cáo y học: "Tetra-O-methyl nordihydroguaiaretic acid (Terameprocol) inhibits the NF-B-dependent transcription of TNF-a and MCP-1/CCL2 genes by preventing RelA from binding its cognate sites on DNA" ppsx
... for TLR-3 and TLR-8 are double and single stranded RNA, respectively In these experiments we used the artificial ligands poly(I:C) for TLR-3 and resiquimod (R-848) for TLR-8 HEK293/TLR3 and HEK293/TLR8 ... for 60 while panels D and F show treatments with LPS and TMP for 20 and 60 min, respectively Following treatment cells were fixed, permeabilized and stained with anti-RelA Ab and a fluorescein coupled ... performed the experiments and drafted the manuscript FS and JRW supervised the reporter assays MLS supervised the ChIP and EMSA experiments SML, FS, and MLS participated in design and coordination of...
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Báo cáo y học: "Common gene expression strategies revealed by genome-wide analysis in yeast" ppt
... blue) between RA and PA, PA and TLR or TLRi, RA and TR and between TR and TLR or TLRi Some of these correlations have been described previously [8,11,17,19] The correlation between TR and TLR was ... PA and TR, RA and TLRi, and PS with all the other variables but RS (numbers in green in Figure 2a) Whereas the PA-TR positive correlation might be explained by the link between TR and RA and ... from Additional data files and and not present in Figure Additional data file 12 is a table listing the standard error averages calculated for experimental (aSEe) and random sampling (aSEr) estimations...
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Expansion and its realization in the short story “Runaway” by Alice Munro from systemic functional grammar perspective = Bành trướng và sự thể hiện của nó trong truyện ngắn Trốn chạy của Alice Munr
... groups as and in that way, 17 similarly, whereas and thus, and thereby, and so, and neither are used to introduce a clause of comparison For example: My sister doesn‟t go to school on Sunday and neither ... then, so, for, but, yet, still, or a combination of and with another conjunctive element such as and then, and thus, and yet, afterwards, and at that time, etc Halliday distinguishes four subtypes ... manner and causal-conditional Temporal enhancement can be signaled by a conjunction or a conjunction group such as and then, and afterwards, first … then (later time), and just then, and at the...
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TRANSITIVITY AND ITS REALIZATION IN THE SHORT STORY “THE NIGHTINGALE AND THE ROSE” BY OSCAR WILDE FROM SYSTEMIC FUNCTIONAL GRAMMAR PERSPECTIVE
... reader to feel and perceive From a very high and small place like this, the Nightingale still feels and shares the student‟s misery And from this empathy, the Nightingale wondered and stuck by ... the animals and trees could speak to one another about true love, sacrifice, and death It is a story that could easily be memorized and retold orally This will provide an easy understanding for ... purchase, and weigh out to describe the indefinite of love The reader seems to feel the mystery of love clearly and specifically with the choice of words and structure like this Relational process and...
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Removal of Cadmium from Aqueous Solutions by Charcoals Prepared from Giant Reed (Arundo donax)
... T., Osada M and Hosomi M (2009b) Nitrogen Dynamics and Biomass Production in a Vertical Flow Constructed Wetland Cultivated with Forage Rice and their Mathematical Modeling, J of Water and Environ ... Huang J and Luo Y.-H (2005) Electrostatic Trapping of Double-Stranded DNA by Using Cadmium Hydroxide Nanostrands, NANO LETTERS, 5(1), 97-100 Li Y.-H., Wang S., Luan Z., Ding J., Xu C and Wu D ... (○), and calculated cadmium adsorbed on charcoal (▲) (The charcoal prepared under 700ºC was used and the dosages of charcoal were 316 mg/L (Left) and 250 mg/L (Right)) - 308 - Journal of Water and...
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Overview of Plant Biotechnology from Its Early Roots to the Present
... evolution of new resistant pests, development of new crop and forest species/cultivars and their products, and changes in market/consumer demands and needs For human health benefits, new technologies ... analysis at the levels of transcripts, proteins, and metabolites and methods for genome modification by both homologous and site-specific recombination Genetic and metabolic engineering are playing a substantial ... in edible and industrial products Lauric oil is ideal for use in the soap and detergent industries, as it is responsible for the cleansing and sudsing properties of shampoos, soaps, and detergents...
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Tài liệu Báo cáo khoa học: "Learning the Latent Semantics of a Concept from its Definition" pptx
... of noun-noun and verb-verb pairs (Sinha and Mihalcea, 2007; Guo and Diab, 2010) Our similarity measure wmfvec exploits the same information (sense definitions) elesk and ldavec use, and outperforms ... select the best of them and combine them into one system Specifically, in their implementation they use jcn for noun-noun and verbverb pairs, and elesk for other pairs (Sinha and Mihalcea, 2007) used ... latent vec- Model random elesk ldavec wmfvec jcn+elesk jcn+wmfvec SE3 random elesk ldavec wmfvec jcn+elesk jcn+wmfvec SE07 random elesk ldavec wmfvec jcn+elesk jcn+wmfvec Semcor random elesk ldavec...
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Tài liệu Báo cáo khoa học: Cloning, characterization and expression analysis of interleukin-10 from the common carp, Cyprinus carpio L. docx
... sequence is closer to human and pufferfish (torafugu and spotted green pufferfish) IL-10 sequences Pufferfish and carp IL-10 genes, clustered together and distant from IL-20 and IL24, as recently determined ... Using Parsimony ( *and Other Methods), 4th edn Sinauer Associates, Sunderland, Massachussetts 24 Laing, K.J., Holland, J., Bonilla, S., Cunningham, C & Secombes, C.J (2001) Cloning and sequencing ... homologues and the torafugu IL-10 genomic sequence, we isolated a clone of 440 bp (resembling mammalian IL-10) from carp head kidney cells stimulated with LPS and concanavalin A [18] The 5¢ and 3¢...
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Tài liệu Báo cáo khoa học: Different modes of dipeptidyl peptidase IV (CD26) inhibition by oligopeptides derived from the N-terminus of HIV-1 Tat indicate at least two inhibitor binding sites doc
... intersection For the calculation of the Ki value and the factors a, c and d the slopes and intercepts were replotted vs [I] according to Eqns (4) and (5), respectively Here, cÆKi represents the ... arrangements of each run of Tat(1–9) and Trp2-Tat(1–9) were saved in a database and subsequently minimized with the standard TRIPOS force field using Gasteiger charges [32] and a distance dependent dielectric ... Gly-Pro-R110CO-(CH2)4Cl [26,27] were synthesized according to standard procedures of peptide synthesis and were purified by HPLC The synthesis and characterization of the DP IV inhibitor Pro-Pro(P)[OPh-4...
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Tài liệu Báo cáo khoa học: Complete subunit sequences, structure and evolution of the 6 · 6-mer hemocyanin from the common house centipede, Scutigera coleoptrata pptx
... 2.1 and 2.2 (CuA site) and 2.5 and 2.6 (CuB site) The subunits all contain the cysteines forming a disulfide bridge that stabilize domain [27] (Figs and 4) One (HcA, HcB, HcC) or two (HcD and ... known This allows us to compare structure, and intra- and intermolecular evolution of hemocyanins from Myriapoda, Crustacea and Chelicerata Materials and methods Protein biochemistry Scutigera ... patterns of the SDS, native and 2D electrophoresis, we assign HcA and HcB to the a and b polypeptides (that could not be separated in [14]), respectively, HcC to the c subunit, and HcD to the d subunit...
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Tài liệu Báo cáo khoa học: Cleavage of nonphenolic b-1 diarylpropane lignin model dimers by manganese peroxidase from Phanerochaete chrysosporium Evidence for a hydrogen abstraction mechanism docx
... oxidation of nonphenolic diarylpropanes (reactions A, C, and E) and a-keto diarylpropanes (reactions B, D, and F) by homogeneous MnP and LiP (G) MnP reactions were carried out in 50 mM malonate ... calibration curves obtained with standards Results Oxidation of diarylpropane substrates by MnP Time courses for the oxidation of diarylpropanes I and VI by MnP, in the presence and absence of Tween 80, ... 20%) and 4-methoxyacetophenone (III, 14%) (Table 1) No detectable amount of a-keto diarylpropane (IV) was Fig Oxidation of the diarylpropanes I (d,s) and VI (m,n) by MnP, in the presence (d,m) and...
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Báo cáo khoa học: Reduction of a biochemical model with preservation of its basic dynamic properties doc
... r and s denote reactions and species, respectively, and ys ¼ Vextracellular ⁄ Vcytosolic ¼ yvol (i.e the ratio of the extracellular volume to the cytosolic) if s is an extracellular species and ... 3) and not call for any action Others have both internal and external parts For example, d½NADH ¼ ¼ vglycerol þ vADH À vGAPDH dt has the external part vADH and the internal parts vglycerol and ... (3) demands that vlpPEP is substituted by vPK or vice versa, and Eqn (4) 4868 demands substitution of vHK, vstorage or vPFK We tested the six possible combinations of solutions to Eqns (3) and (4)...
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Báo cáo khoa học: Tumor necrosis factor-a converting enzyme is processed by proprotein-convertases to its mature form which is degraded upon phorbol ester stimulation pptx
... 1.5–6 h the cells were harvested and cellular membranes were isolated Mature and immature forms of endogenous TACE and ADAM10 were detected by Western blot analysis and quantified as described under ... on the processing of endogenous TACE and ADAM10 and on the APPsa release from HEK293 LoVo and SHY-5Y cells (A) Proteolytic processing of endogenous TACE and ADAM10 in PMA-treated cells Cells ... esters Taken together, both TACE and ADAM10 possess a-secretase activity and are proteolytically activated by PC7 and furin Furthermore, a furin-independent and PMA induced disappearance of mature...
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Báo cáo khoa học: "A Method for Relating Multiple Newspaper Articles by Using Graphs, and Its Application to Webcasting" pptx
... Applications in Knowledge Navigation and Retrieval N Jardine, and R Sibson 1968 The Construction of Hierarchic and Non-Hierarchic Classifications Computer, pages 177-184 I Mani and E Bloedorn 1997 Multi-document ... differentia word for dT, and is in its adjacent articles ds, dg, anddlo This means that d7 is a starting point of the new topic "state." Such words are called topic words, and are represented in ... readily understandable way Software tools for handling graphs are also required Formal features of graphs can express the underlying characteristics of articles More efficient and useful algorithms...
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Tìm thêm: xác định các mục tiêu của chương trình xác định các nguyên tắc biên soạn khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản xác định thời lượng học về mặt lí thuyết và thực tế tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra đối với đối tượng giảng viên và đối tượng quản lí điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu nội dung cụ thể cho từng kĩ năng ở từng cấp độ phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ lồng sóc mở máy động cơ rôto dây quấn các đặc tính của động cơ điện không đồng bộ đặc tuyến hiệu suất h fi p2 đặc tuyến dòng điện stato i1 fi p2 động cơ điện không đồng bộ một pha phần 3 giới thiệu nguyên liệu chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008