... Generalization and Refinement Phrases are acquired in a process of hypothesis formation and error correction. The program generates and refines hy- potheses about both the linguistic pattern, and the ... of each phrase. This paper is concerned in the representation of phrase meaningsand the process of acquiring these meanings from examples in context. * This research was supported in part ... request, and on the other hand, John read the riot act implies that he reacted angrily about a certain event in the past. John gave Mary a hard time implies that he re- fused to cooperate, and...
... (AND (DEFINITION CUP N CuP) (OR (AND (OR (A~D (DEFINITIONIt~RY N (IT It~RY)) (DEFINITIONTO P (TO 70))) (AND (DEFINITION MARY N MARY) (DEFINITION TO P (TO (AT ?0))))) (OR (AND (OR (AND ... lexical categories N, V and P, the English pa- rameters HEAD-first and SPEC-first and the English auxiliary system. 2A more principled way of deriving the rides for S and from T-theory is given ... (PATH 71 (FROM ?2))))))) (AND (DEFINITION MARY N MARY) (DEFINITION TO P (AT 70)) (OR (AND (OR (AND (DEFINITION JOHN N (AT JOHN)) (DEFINITION FROM P (FROM ?0))) (AND (DEFINITION JOHN N JOHN)...
... with modality and modal verbsin English and in Vietnamese.Chapter two is focused on investigating the meanings expressed by can, may, must and oncontrastive analysis of the meanings expressed ... as I (but not other person) give youpermission to come and sit and it is used in a formal situation between Jane Eyre and Mr. Rochester. And can in (79) also expresses the idea of having permission. ... không?- Đ ợc, chị ạ. (1: 182)( and have you learnt French?" "Yes, Bessie, I can both read it and speak it." " ;And you can work on muslin and canvas?" "I can."...
... Asn117, Tyr287 and Arg311), and the two Cys residues (105 and 186) thatform a disulfide bond between EL1 and EL2 to maintain thereceptor in a high affinity conformational state. Several Ser and Thr ... subtypes (Fig. 3).The candidate residues interacting directly with TRH areTyr106 and Asn110 in TM3, Tyr282 in TM6, and Arg306 inTM7 (in Xenopus and mouse TRHR1) [41]. Tyr106 and Asn110 have been ... positions (3 and 10), whilexTRHR2 had these sites at positions 3 and 12. Theglycosylation site in EL2 (Asn167 for xTRHR1 and Asn172for xTRHR2) and the two homologous Cys residues (335 and 337 for...
... ofdesferrioxamines B and E (labelled as DesB and DesE) and complementation of desferri-oxamine production. (A) Cultures of S. coeli-color A3(2) and the TAdmdR1,TAadm and DdmdR1 ⁄ adm mutants ... pHZdmdR1)-resistant colonieswere selected and assayed for siderophore and antibioticproduction.Assays of actinorhodin and undecylprodigiosinUndecylprodigiosin and actinorhodin production weremeasured ... antiparallelgenes are translated, and to study the involvement ofdmdR1 and the antisense gene (adm) in the regulationof S. coelicolor A(3)2 sporulation and antibiotic and siderophore biosynthesis.ResultsThe...
... variants giving 169 and 246 bp fragments. SalI cuts theamplification product of ml2p, giving 66, 121 and 243 bp fragments, and the amlpification product of ml3p and ml3.1p, giving 50 and 365 bpfragments. ... 5¢-GGTGAGAACGCAGTCAGATGCTAGG-3¢ and PstI, and the second round with another specificprimer 5¢-GACCGGATCCCTCTGGGTAGAGAG-3¢ and PstI. The product of the second round of PCR wascloned and the 1066 bp sequence encoding ... SDS/polyacrylamidegels, the recombinant ML1p and ML2p A-chains could beseen as a single band 30 kDa. The recombinant ML3p and ML3.1p A-chain preparations always gave an addi-tional band of 60 kDa (which may...
... numbersAB061234 and AB064661 respectively. The ORFs ofHRT1 and HRT2 encode 290 and 284 amino acid residues(87.3% identity) with predicted molecular masses of 33.2 and 32.8 kDa respectively. Hydropathy and ... primers(2), are shown.Fig. 4. Overexpression of HRT1 and HRT2 in E. coli and purificationof HRT2. The pETHRT1 and pETHRT2 were constructed and introduced into E. coli BL21(DE3). The expression ... (AAM92883,AAM92884, AAM92885, AAM92887, AAM92888),BAB92024 and AAM92882 (AAM92886) (submitted toGenbankTM and DDBJ by Coldren et al. and Sando et al.respectively). However, most of these amino...
... statictypability, the lemma holds.Intro F and Intro F : and F , so and F are con-sistent. By bounded completeness, F and F . By upward closure,F F and by static typability,F F F F .Furthermore, ... two BCPOs, and ,is a classical join-preserving encoding ofinto iff:injectivity is an injection,zero preservation2iff, and join homomorphism, where they exist.Join-preserving encodings ... between BCPOs for which no classical join-preserving encoding exists.zero preservation for all and , iff , and join homomorphism for all and , , where they exist.When maps elements of to singleton...
... amidohydrolase (44.8% identity and 51.2% similarity) and N-acyl-D-asparate amidohydrolase(48.5% identity and 56.5% similarity) from Alicaligenesxylosoxydans ssp. xylosoxydans A-6 and theD-aminoacylasefrom ... theenvironment and the gene for the enzyme was cloned and then sequenced. The recombinant protein, N-D-AAase, wasalso produced in the Escherichia coli, purified and charac-terized.MATERIALS AND METHODSBacterial ... NewBioLabs and Gibco BRL. Pfu DNA polymerase and alkaline phosphatase were from Promega and BoehringerMannheim, respectively.D-Amino acid oxidase (EC 1.4.3.3)from porcine kidney and horseradish...
... peptides, e.g. GnIH [9], fGRP[11] and RFRPs [12–15,23] and other RFamide peptides,e.g. PrRP [8] and neuropeptide FF [7]. Further experimentsare needed to understand the possible multiple regulatoryfunctions ... (UCSF) and a corresponding peak was furtherexamined in a tandem MS analysis. The needle voltage wasoptimized at 1000 V and the cone voltage was set at 50 V.Argon was used as the collision gas and ... cDNA encoding the precursor polypeptide and identification and localization of the mature peptideKaori Sawada1,2, Kazuyoshi Ukena1,2, Honoo Satake3, Eiko Iwakoshi3, Hiroyuki Minakata3and...
... asthe standards and their elution volumes, Ve, were deter-mined. The standard curve was plotted with the logarithmof molecular mass against Ve/V0of the standard protein.Peptidase and ATPase ... mMNaCl, 10 mMMgCl2 and 50 mMTris/HCl, pH 8.0, for 20 min with plasmidDNA (500 ng) at the indicated temperatures. Analysis usedstandard 0.8% agarose gels, and DNA bands were visual-ized ... withmaxima at 210 and 222 nm, suggesting a major a-helicalsecondary structure in itself (Fig. 8A). The deconvolution ofthis spectrum yielded 40% a-helix, 30% b-sheet, and 30%random coil and was similar...
... transcript levels and enzyme a ctivities w ere f ound inroots and flowers, while the m RNA level and activity w erelowinstemsandleaves.Moreover,whentestedinmethyljasmonate- and elicitor-treated ... have been characterized at abiochemical and molecular level and the encoding geneshave been cloned. Biosynthesis takes place mainly inchloroplasts and peroxisomes, initiating after the releaseof ... generating overlapping fragments after5¢ -and3 ¢-RACE, respectively. Sequencing and annealing ofthe 5¢ and the 3¢ sequence revealed an ORF of 789 bp, encoding a 262 amino acid protein (Fig. 1)....
... relevant and non-relevant subsets, and α, β, γ ∈R are constants. Salton and Buckley (1990) reportbest results using β = 0.75 and γ = 0.25.The positive feedback part of this process ha sbecome standard ... ScienceLaboratories, Nippon Telegraph and Telephone Corpo-ration and CSLI, Stanford University, and by EC/NSFgrant IST-1999-11438 for the MUCHMORE project.Ribiero-Neto, 1999), and from this original appli-cation ... acquisition (Landauer and Dumais, 1997 ; Widdows, 2003) and disambigua-tion (Sch¨utze, 1998). One benefit of these models isthat the similarity between pairs of terms or betweenqueries and documents...
... Encyclopedia) and on future plans for MindNet. For additional details and background on the creation and use of MindNet, readers are referred to Richardson (1997), Vanderwende (1996), and Dolan ... for 3 European and 3 Asian languages: French, German, and Spanish; Chinese, Japanese, and Korean. The syntactic parsers for some of these languages are already quite advanced and have been ... correct Dissimilar correct 84% 82% Human benchmark: random sample of 200 similar and dissimilar word pairs were evaluated by 5 humans and by MindNet: Similar correct Dissimilar correct Humans:...
... NICN1 fragment after 24, 26, 30, 34 and 38 cycles ofPCR is shown. The NICN1 amplification was performed with identicalprimers and conditions on the human and murine cDNA panel. In thelower ... instruction. For the human and murine NICN1 RT-PCR, a fragment of 194 bp wasamplified using the primers NICN1_V362 (5¢-CATCACCACTGTGGCTGTC-3¢) and NICN1_R555(5¢-CTCTGTCAGTGCCCACATC-3¢) and an annealingtemperature ... days 8.5, 9.5 and 10.5 were dissected in NaCl/Pi and fixed overnight at 4 °C in 4% paraformaldehyde. Afterwashing once with NaCl/Pithe embryos were dehydratedthroughamethanolseriesandstoredin100%methanolat)20...