... rich chemistry, temperature and highpressure prevailing around the deep-sea vents offer a plausible environment for the emergence of life [35] High hydrostatic pressures (up to 200 MPa) were ... the hairpin ribozyme subjected to high hydrostatic pressures is completely recovered upon returning to atmospheric pressure, indicating that the effects of pressure are fully reversible, as was ... steady state studies of enzymatic reactions at high pressure: tests with aspartate transcarbamylase Anal Biochem 187, 258–261 51 Heremans K (1982) Highpressure effects on proteins and other biomolecules...
... 000 g during at 15 C prior to the SANS experiments High- pressure cell During the SANS experiments the protein solutions were contained in a high- pressure cell made of stainless steel with two parallel ... function of pressure, P moderate pressures near 300 MPa induce subtle structural rearrangements of the protein matrix whereas higher pressures, near GPa, lead to unfolding Pressure may also induce changes ... Heremans, K., Frank, J., Masson, P & Balny, C (1996) High- pressure eects on protein structure and function Proteins 24, 8191 Heremans, K (1982) High- pressure eects on proteins and other biomolecules...
... solution at the beginning indicating that P420 was not formed during the high- pressure stopped-flow experiment The high- pressure stopped-flow apparatus used is interfaced with the Aminco DW2 spectrometer ... (see below) Ó FEBS 2002 High- pressure stopped-flow for P450 CO binding (Eur J Biochem 269) 2991 Three shots at each pressure were taken and fitted The kon values for the same pressure were averaged ... 20 21 22 23 24 25 26 27 28 hemeproteins High- pressure study J Am Chem Soc 112, 6880– 6886 Unno, M., Ishimori, K., Ishimura, Y & Morishima, I (1994) High- pressure flash photolysis study of heme...
... country-years in the highest public debt/GDP category) has a major effect on the computed average in the highest public debt/GDP category It reduces the average growth for New Zealand in the highest public ... real growth in the high public debt category is +2.2 percent per year compared to the −0.1 percent per year published in RR The actual growth gap between the highest and next highest debt/GDP categories ... the highest public debt/GDP category with only 71 country-years of data: 25 years of Belgium were dropped in addition to the 14 already All of these cases would contribute observations to the highest...
... ATP-G-actin [20] Highpressure apparatus Highpressure devices used for this study consisted of a thermostated highpressure vessel equipped with sapphire windows and a pump capable of raising pressure ... then reached a plateau On a further increase in pressure, it decreased gradually in a relatively lower pressure range and steeply in a higher pressure range At 400 MPa it dropped almost to the ... transformation (F«G equilibrium under a certain pressure) is thought to occur without denaturation in the pressure range used (see Fig 12) In a higher pressure range, above 250 MPa (Fig 9), it was...
... 220 MPa or higher It was con®rmed that the excitation spectra of BIPM and FITC not change under these high pressures Hydrogen±tritium exchange under highpressure Effects of highpressure on ... The buffer used in the highpressure experiments were 40 mM Tris/HCl, pH 7.4, to minimize the pressure- induced pH changes [25] In situ spectroscopic measurements under highpressure Absorbance and ... increasing pressures are shown in Fig 2A The value for kmax was shifted from 341 to 345 nm with increasing pressure, up to 220 MPa, and then decreased for pressures of 220 MPa or higher, being...
... we used a high- pressure technique Highpressure is currently used in many areas of biotechnology Its mechanism of action includes the decisive role of water structure [26,27] Highpressure allows ... aminoacylation reaction of tRNA at highpressure remains unknown Recently, we obtained new information about the conformation of tRNA at elevated pressure [26] It is known that highpressure lowers the pH ... nucleophilic attack (Fig 10) The entire process is induced by highpressure and does not proceed without it We showed that highpressure influences the conformation of tRNA because of rearrangements...
... controlled and reduced cement leakage the pressure drops more gradually allowing the highpressure to be retained for longer The time with high cementation pressure (250 kPa) was about twice that ... conventional pressuriser tested here can reach a high pressure, nearly 300 kPa (2256 mmHg), but due to cement leakage did not retain this pressure The changes of pressures depend of how the pressurisers ... cement pressurisation lead to increased cement penetration [6,7,11-16] while constant highpressure benefits high viscosity cement penetration whether in a constrained cavity such as the femur...
... 1974 EURASIP Journal on Applied Signal Processing Mic Nozzle Waterjet Borehole Mine Figure 1: A high- pressure waterjet rapidly bores a hole through the soil to strike a buried object The impact ... technology is investigated as a confirmation sensor for landmine location and discrimination A high- pressure waterjet, fired at soil, will quickly create a borehole in the soil (Figure 1) If the ... to set off a landmine If needed, even less force can be used by decreasing the pressure or nozzle size Depending on pressure, nozzle diameter and firing time, the waterjet can penetrate up to 12...
... pressurisation of root systems [6], the root pressure probe [18], the potometer [29], the negative pressure flow system for root sections [14], the highpressure flowmeter (HPFM) [25, 27] Each method ... recording flow and pressure; k is calculated from the linear regression slope of flow versus applied pressure It should be noticed that xylem vessels are refilled by the highpressure water perfusion ... the highpressure flow meter in crop plants, J Exp Bot 51 (2000) 823–828 [25] Tyree M.T., Patino S., Bennink J., Alexander J., Dynamic measurements of root hydraulic conductance using a highpressure...
... to the highpressure flowmeter described below, all cut surfaces were shaved with a razor blade to remove blockage of cut vessels by cell-wall fragments formed by the initial cuts The high- pressure ... a differential pressure gauge like that used in a lowpressure flow meter described by Tyree (1983) However, that would have eliminated the main advantages of the present high- pressure flow meter, ... a pressure reservoir (R) Water contained in the reservoir was distilled water filtered through an 0.1 μm filter The water was placed under pressure by compressed air, controlled with a pressure...
... NT-proBNP level with cardiac dysfunction In a seminal paper, Charpentier and coworkers [5] reported higher BNP levels in septic patients with altered left ejection fraction In the study by Mokart ... upregulation of BNP and NT-proBNP secretion, whereas cardiac dysfunction could contribute to persistent high NTproBNP levels A limitation of the study by Mokart and coworkers is the lack of serial measurements...
... antihypertensive medication irrespective of high blood pressure Furthermore, an effective blood pressure treatment and an ineffective blood pressure treatment, meaning high blood pressure despite the use of ... high blood pressure Furthermore, the multivariable regression analysis revealed a significant interaction between gender and high blood pressure This might possibly indicate that high blood pressure ... medical treatment have a higher underlying blood pressure compared to effectively treated subject Moreover, the effects of high blood pressure and antihypertensive medication are highly correlated A...
... Declaration of Helsinki High- intensity NPPV All patients were hospitalized to establish high- intensity NPPV The assist/control mode is used for high- intensity NPPV, preferably in a pressure- limited ... positive airway pressure, EPAP = expiratory airway pressure, bf = breathing frequency; SD = standard deviation Table Blood gas levels, lung function parameters, mouth occlusion pressures, hemoglobin ... tolerate high- intensity NPPV would result in better outcomes Therefore, prospective trials also assessing the number of patients not tolerating high- intensity NPPV are required Thirdly, high- intensity...
... temperature is shown in Fig It increases the water temperature, and the vapor pressure increases Due to the increase in the vapor pressure of mercury, the electron temperature decreases, leading to a ... divagation at a small flow - 72 - Journal of Water and Environment Technology, Vol.3, No.1, 2005 rate does not arise Distributed installation of the sewage effluent disaffection system for wastewater ... of Electrodeless UV Lamp The temperature of the coldest spot of the lamp determines the Hg vapor pressure One can therefore use that temperature as a parameter representing Hg vapor density in...
... cold inactivation of CPY under highpressure Fig Change in CPY activity following the release of pressure Enzymic activities of CPY, which was exposed to 400 MPa pressure at 10 °C for 60 (s), ... under highpressure (Eur J Biochem 269) 4671 DISCUSSION Unique properties of CPY CPY exhibits a typical pressure- dependent cold inactivation phenomenon When both subzero temperature and pressure ... 2002 Cold inactivation of CPY under highpressure (Eur J Biochem 269) 4673 suggests the combined application of urea or GdnHCl with subzero temperature and highpressure for protein chemistry field...
... rate of nanowires is always higher than the deposi˚ tion rate (2 A/s) in the interval of T from 460 to 590 °C, has a maximum around 550–580 °C and rapidly decreases at higher T due to adatom desorption ... the growth is controlled by the direct impingement of material to the drop It normally happens at high surface temperatures and, consequently, small diffusion lengths of adatoms When the growth temperature ... crystal and Ceq(T) is the equilibrium concentration of alloy As shown in Ref [24], if U is much higher than the alloy supersaturation f, the wire growth is mainly controlled by the transport of...
... for high fat coconut milk at different homogenization pressures and sterilizing temperatures Temperature ( C) Homogenization pressure (MPa) K (Pa sn) n r2 Temperature ( C) Homogenization pressure ... the high shear forces acted on Table Effect of homogenization pressure on fat particle diameter (Dm) of canned high fat coconut milk Temperature ( C) Homogenization Fat particle diameter pressure ... (109.3–121.1 C under pressure of 5–15 psi) on the stability of canned high fat coconut milk (30%) The information obtained from the study could be used as a guideline for developing of high fat coconut...