... pAM889 pAM8 90 pAM891 pAM892 pAM895 pAM896 pAM899 pAM 902 pAM 903 pAM 904 pAM 905 pAM 906 pAM 907 pAM 908 pAM 909 pAM9 10 pAM912 pAM913 pAM914 pAM915 pAM918 pAM919 pAM 100 1 pAM 100 2 pAM 100 3 Vector for expression ... polyclonal GFP-speci c antiserum was a gift from J Kahana and P Silver (Dana Farber Cancer Center, Boston, MA) The anti-actin mAb was MAB1 501 from Chemicon International (Temecula, CA) The anti-hexokinase ... for actin-patch formation at polarized cortical sites [29] Because cortical actin patches are short-lived structures, continual actin-patch formation at polarized cortical sites is essential for...
... 809 The Role of the IDbCommand Interface 809 The Role of the IDbDataParameter and IDataParameter Interfaces 8 10 The Role of the IDbDataAdapter and IDataAdapter Interfaces ... 100 1 Building a WCF Service 100 2 The [ServiceContract] Attribute 100 4 The [OperationContract] Attribute 100 5 Service Types As Operational Contracts ... Reflection, Late Binding, and Attribute-Based Programming.555 Chapter 16: Dynamic Types and the Dynamic Language Runtime .599 Chapter 17: Processes, AppDomains, and Object Contexts 623 Chapter...
... signal peptide, was amplified by PCR to incorporate BamHI and XhoI sites at either end, using the following primers: forward, 5¢-CGCGGATCCTGGTCTTTCCAAAATATTC AGGCCA-3¢ and reverse, 5¢-GTCCTCGAGGTACATCA ... rehydrated actively for 12 h Isoelectric focusing was performed (Protean IEF Cell, Bio-Rad): 2 50 V (1 h), 500 V (for h), 100 0 V (for h, 10 000 V (gradient over h), and 10 000 V (for 90 000 Vh) at 20 C ... silver staining of plant proteins, RNA and DNA in polyacrylamide gels Electrophoresis 8, 93–99 Biswas C, Sinha D & Mandal C ( 200 0) Investigation of Achatinin, a 9-O-acetyl sialic acid-binding lectin,...
... Investigaciones Cientı´ ficas y ´ ´ Tecnicas (CONICET), Secretarı´ a de Ciencia y Tecnica de la Univer´ ´ sidad Nacional de Cordoba y Agencia Cordoba Ciencia del Gobierno ´ de la Provincia de Cordoba, ... y Tecnologica de la Secretarı´ a de Ciencia y Tecnologı´ a del Ministerio de Cultura y ´ ´ ´ Educacion en el marco del Programa de Modernizacion Tecnologica (BID 802 /OC-AR), Consejo Nacional de ... were inactivated by addition of mL 5% trichloroacetic acid and heated at 90 C for 15 Radioactivity bound to protein was measured in hot-trichloroacetic acid-insoluble material as described previously...
... GdnHCl and urea when electronic interactions play a role in their stability Conformational changes at low chemical denaturant concentrations – structural and functional implications The elucidation ... intermediate accumulated between 0. 5 and m GdnHCl The intrinsic Trp fluorescence and extrinsic ANS fluorescence data indicated that GdnHCl-induced PARN unfolding involved at least two intermediates accumulated ... domain of poly (A) -speci c ribonuclease has a noncanonical binding site for mRNA cap analog recognition Nucleic Acids Res 36, 4754– 4767 Monecke T, Schell S, Dickmanns A & Ficner R ( 200 8) Crystal...
... approximately 263 nm anda negative band at approximately 2 40 nm, whereas antiparallel G4 structures, such as basket and chair forms, show two positive bands at approximately 295 and 2 40 nm and ... maintained at D2 20 3 30 in the range 0. 2 0. 8, and the CD data represent three averaged scans taken at an experimental temperature (25– 90 C) All CD spectra are baselinecorrected for signal contributions ... grants from the Slovak Grant Agency (1 ⁄ 1274 ⁄ 04 and ⁄ 3254 ⁄ 06 ) and the Science and Technology Assistance Agency (APVT 20- 006 604 ) I would like to thank Gavin Cowper and Lenka Sieber for critically...
... in standard aminoacylation reactions Km and kcat values with respect to both tRNA and serine (Km ¼ 51 ± lm and kcat ¼ 0. 47 ± 0. 02 s)1 for serine; Km ¼ 0. 65 ± 0. 07 lm and kcat ¼ 0. 54 ± 0. 02 s)1 ... temperature, and checked periodically for the appearance of a blue color that developed between 30 and h b-Galactosidase activity was quantified using Gal-ONp as a substrate in assays carried out according ... synthesis quality control in Archaea Proc Natl Acad Sci USA 101 , 102 60 102 65 29 Dagkessamanskaia A, Martin-Yken H, Basmaji F, Briza P & Francois J ( 200 1) Interaction of Knr4 protein, a protein...
... bi-glycosylated and the 18 00 0-Da band as monoglycosylated (Fig 2) The deglycosylated 14 400 -Da band may correspond to the truncation of about 20 amino acids in the embryonic IFN -c molecule, as ... that the structural and chemical characteristics of TrIFN -c affects its bioavailability and biological effect(s) on the maternal uterus In particular, this shortened version of IFN -c, lacking a ... mgÆmL)1 of a- cyano-4-hydroxycinnamic acid matrix in 50% (v/v) acetonitrile and0. 1% (v/v) trifluororacetic acid For acquisition, the accelerating voltage used was 20 kV Peptide spectra were recorded...
... were analyzed after dephosphorylation for (lanes and 7), (lanes and 8), 10 (lanes and 9), and 20 (lanes and 10) antibody against Cdc37 and the only band that became radioactive after incubation ... Journal 274 ( 200 7) 56 90 5 703 ª 200 7 The Authors Journal compilation ª 200 7 FEBS Y Miyata and E Nishida Cdc37 phosphorylation by CK2 and signaling kinases A B C D E F Fig Subcellular localization ... [19,21], which constitutes a consensus Fig Phospho-speci c antibody against an Hsp 90 cochaperone, Cdc37 (A, B) Recombinant Cdc37 was incubated at 30 C for 30 alone (lanes 1–3), with CK 2a (lanes 4–6),...
... http://www.nov agen.com/SharedImages/TechnicalLiterature/7_tb055.pdf Datta, A. B., Chakrabarti, P., Subramanya, H.S & Parrack, P ( 200 1) Purification and crystallization of CII: an unstable transcription activator ... Oppenheim, A. B ( 200 2) The phage CII transcriptional activator carries a C- terminal domain signaling for rapid proteolysis Proc Natl Acad Sci USA 99, 14964–14969 Novagen ( 200 2) pET System Manual, 10th ... 661–665 Pal, D., Mahapatra, P., Manna, T., Chakrabarti, P., Bhattacharyya, B., Banerjee, A. , Basu, G & Roy, S ( 200 1) Conformational properties of a- tubulin tail peptide: implications for tail–body...
... that the apoptotic effects of the biologically active compounds Act–ActV are associated with cell cycle perturbations, in which similar cell cycle changes, characterized by accumulation of cells ... analysed by flow cytometry Cell cycle analysis was carried out using CELLQUEST (Becton Dickinson) software A total of 10 000and 20 000 cells were characterized by flow cytometry for apoptosis and ... energy as kcalÆmol)1 Drug–DNA complexation Helix–coil transition Sample Tm DT DH DNA 83.3 10. 5 7. 50 ActII–DNA 105 .0 21 .0 12.3 ActIII–DNA 101 .3 14.5 10. 4 ActIV–DNA 99 .0 12 .0 10. 0 ActV–DNA 97.3...
... exchanger Concept of direct- sizing in contraflow Direct- sizing of a contraflow exchanger Best of rectangular and triangular ducts Best small, plain rectangular duct Fine-tuning of ROSF surfaces ... each section using spline-fitted data CALCULATE SURFACE AREA AND LENGTH for each section CALCULATE PRESSURE LOSS FOR EACH FLUID for each section SUM LENGTHS AND PRESSURE LOSSES to obtain final ... Advances in Thermal Design of Heat Exchangers Related Titles Combined Power and Process - An Exergy Approach F J Barclay 8 605 8 129 Optical Methods and Data Processing in Heat and Fluid...
... the char and s appending the [ 10] we have an array of 10 characters But, the name multi[5] is itself an array indicating that there are elements each being an array of 10 characters Hence we have ... data type Thus in: typedef int Array[ 10] ; Array becomes a data type for an array of 10 integers i.e Array my_arr; declares my_arr as an array of 10 integers and Array arr2d[5]; makes arr2d an ... array of characters, with the last character being a ' \0' What we have done above is deal with copying an array It happens to be an array of characters but the technique could be applied to an array...
... the IDbDataParameter and IDataParameter Interfaces 766 The Role of the IDbDataAdapter and IDataAdapter Interfaces 767 The Role of the IDataReader and IDataRecord Interfaces ... Interoperability (Apress, 200 2), Visual Basic NET and the NET Platform: An Advanced Guide (Apress, 200 1), and the award-winning C# and the NET Platform (Apress, 200 3) Andrew has also authored ... xxxix PART ■■■ CHAPTER CHAPTER PART CHAPTER CHAPTER CHAPTER CHAPTER CHAPTER CHAPTER CHAPTER CHAPTER CHAPTER CHAPTER CHAPTER CHAPTER CHAPTER The Philosophy of NET ...