... TABLE OF CONTENTS Development of Hematopoietic Repopulating Cells from EmbryonicStemCells The In Vitro Differentiation of Mouse EmbryonicStemCellsinto Neutrophils 10 Development and Analysis of ... from Murine EmbryonicStemCells 11 Development of Lymphoid Lineages EmbryonicStemCells In Vitro from 12 Probing Dendritic Cell Function by Guiding the Differentiation ofEmbryonicStemCells 13 ... Section I Differentiationof Mouse EmbryonicStemCells [1] Lineage Specific Differentiationof Mouse ES Cells: Formation and Differentiationof Early Primitive Ectoderm-like (EPL) Cells By JOY...
... analysis of developmental events was made possible with the isolation ofembryonicstemcells Extensive investigation of the properties and capabilities of these cells has led to the development of ... stimulation of regulatory T (Treg) cells in the host This mechanism in part can be attributed to the differentiationof the mixed pool of donor haematopoietic cellsinto allogeneic antigen-presenting cells ... stages ofembryonic development from fertilization to E6.5 are represented in this image EmbryonicStem (ES) cells are derived from the inner cell mass while epiblast stemcells (EpiSCs) are of epiblast...
... study ofembryonicstemcells TE N Hattori and K Shiota ICM PGC TS cells Placental cells 77 ES cells 49 EG cellsEmbryoniccells Fig Epigenetic distances between ES cells and other stemcells ... establishment ofembryonicstem (ES) cells, which maintain the ability to form all types ofcells in the body, and can differentiate into a variety of cell types in vitro [8] The use of ES cells in ... ED between ES cells and EG cells, the ED of TS cells to ES cells (77) is greater than that of EG cells to ES cells (49), confirming the similarity of EG cells to ES cells DNA methylation profiles...
... chondrogenic differentiationof mesenchymal stemcells (MSCs) The gene-expression (MSCs) profile of mesenchymal stemcells (MSCs; n = 2) was analysed by real-time PCR during their differentiation towards ... Figure Time-course expression of chemokines and their receptors during chondrogenic differentiationof mesenchymal stemcells (MSCs) The gene(MSCs) expression profile of MSCs (n = 2) was analysed ... metalloproteinases in the differentiation and biological 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 characterization of mesenchymal stemcellsStemCells 2006, 24:475-481 Charo IF, Ransohoff RM: The...
... promotes self-renewal of mouse embryonicstemcells Dev Biol 1999, 210:30-43 Li Z, Theus MH, Wei L: Role of ERK 1/2 signaling in neuronal differentiationof cultured embryonicstemcells Dev Growth ... Derivation of midbrain dopamine neurons from human embryonicstemcells Proc Natl Acad Sci USA 2004, 101:12543-12548 Ying QL, Stavridis M, Griffiths D, Li M, Smith A: Conversion ofembryonicstemcells ... detect the Sox1-GFP+ cells from day to day (Fig 3A) The number of Sox1+ cells became 20% of total cells on day and reached the plateau, 50% of total cells, on day Treatment of FGF1 consistently...
... autologous cells as well as allogenic and xenogenic cells [13-16] There are some reports that use totipotential embryonicstemcells in tissue engineering of bone [17,18] Embryonicstemcells (ESCs) ... mouse embryonicstemcells Cloning StemCells 2004, 6(4):386-391 Smith AG, Heath JK, Donaldson DD, Wong GG, Moreau J, Stahl M, Rogers D: Inhibition of pluripotential embryonicstem cell differentiation ... vitro differentiationofembryonicstemcellsinto mineralized osteoblasts Differentiation 2003, 71(1):18-27 Buttery LD, Bourne S, Xynos JD, Wood H, Hughes FJ, Hughes SP, Episkopou V, Polak JM: Differentiation...
... Characterisation of canine adipose tissue derived mesenchymal stemcells In order to demonstrate that the cells isolated from the canine adipose tissue (Figure 1A) are indeed mesenchymal stemcells (MSCs) ... effects of curcumin inhibit the induction of degradative and inflammatory pathways by IL-1β through revoking activation of caspase-3, production of COX-2, phosphorylation of Iκ-Bα and inhibition of ... inhibits chondrogenic differentiationof MSCs in OA and the effect of curcumin Trauma, inflammation or a combination of both (1) lead to the production and accumulation of high levels of pro-inflammatory...
... Refinement and increased efficiency of these two ESC protocols brings the potential to offer a reliable supply ofembryonicstemcells without production of whole embryos or compromising extant ... scoring system Stem cell research: social and political factors Public sharing of information about the basic science of ESCs has proven to be important, since those who are aware of the stem cell ... Human embryonicstem cells: theoretical approaches Blastomere biopsy and culture Prior to the blastocyst stage, a human embryo at 2–3d post fertilization consists of just 4–8 cells, all of which...
... vitro differentiationof mouse embryonicstemcells to hematopoietic cells on an OP9 stromal cell monolayer Methods Enzymol 365, 72–83 21 Strahl BD, Ohba R, Cook RG & Allis CD (1999) Methylation of ... both hematopoi- Fig Sequential activation of globin gene transcription during in vitro erythroid differentiationof murine embryonicstemcells PCR analysis of DNase I treated and reverse-transcribed ... Transcription of LCR hypersensitive site in undifferentiated murine embryonicstemcells and in human CD 133+ bone marrow cells (A) Transcription of LCR regions and the bmaj-globin gene during differentiation...
... expression pattern of undifferentiated and differentiated human embryonicstemcellsStemCells Dev 2007, 16(6):1003-1016 Page 15 of 17 (page number not for citation purposes) Journal of Translational ... expression profiling of single whole embryonicstemcells Nucleic Acids Res 2006, 34(2):e9 Chen C, Ridzon D, Lee CT, Blake J, Sun Y, Strauss WM: Defining embryonicstem cell identity using differentiation- related ... 13 The expression of miR-92 has been reported in human embryonicstem (ES) cells [16,26], mouse ES cells[ 20] or Page 12 of 17 (page number not for citation purposes) Journal of Translational Medicine...
... vitro differentiationof size-sieved stemcellsinto electrically active neural cellsStemCells 2002, 20(6):522-529 Tzeng SF, Tsai MJ, Hung SC, Cheng H: Neuronal morphological change of size-sieved ... infected cells for 48 hours Results FN and LAM enhance differentiationof MSCs into insulin producing cells To examine the effects of ECM, FN and LAM on IPC differentiation by MSCs, a four-stage differentiation ... stromal cells differentiate into neural cells in vitro Exp Neurol 2000, 164(2):247-256 Lumelsky N, Blondel O, Laeng P, Velasco I, Ravin R, McKay R: Differentiationofembryonicstemcells to...
... triggers differentiationof HUMSCs into cardiomyocytes and maturation of HUMSCs derived cardiomyocytes Admittedly, the detailed mechanism(s) of above effects of S1P on differentiationof and maturation ... with Regard to the Source of Fetus-Derived StemCellsStemcells 2007, 25:2886-2895 Wu KH, Mo XM, Zhou B, Lu SH, Yang SG, Liu YL, Han ZC: Cardiac potential ofstemcells from whole human umbilical ... fluorescent immunostaining of aactinin ofcells from three groups, while, J-L shows the fluorescent immunostaining of MHC ofcells from these groups after and 10 days’ culturing Cells from CMCM and...
... embryonicstem cell; H3K4me1, methylation of lysine of histone H3; H3K4me3, trimethylation of lysine of histone H3; H3K27ac, acetylation of lysine 27 of histone H3; H3K27me3, trimethylation of ... epigenomic profiling of a cohort of human embryonicstem cell (hESC) lines can be used to generate hESC reference epigenomes (left panels) The extent of reprogramming and differentiation potential of individual ... cell; hESC, human embryonicstem cell; H3K4me3, trimethylation of lysine of histone H3; H3K27ac, acetylation of lysine 27 of histone H3; H3K27me3, trimethylation of lysine 27 of histone H3; iPSC,...
... Human EmbryonicStemCells (hESCs)…………………………………… 2.2.1 2.3 Expansion of hESCs……………………………………………… Differentiationof hESCs into chondrogenic lineage……………………… 10 2.3.1 Direct chondrogenic differentiation ... MODEL SYSTEM…………………………………………… 65 4.1.1 Pluripotency of human embryonicstemcells …………………… 65 4.1.2 Effects of culture conditions in modulation of chondrogenesis…….66 4.1.3 Effects of culture ... chondrogenic cells Fig 22 Tumorigenicity of hESC-derived chondrogenic cells Fig 23 ECM modulation of post-expansion differentiation capability of hESC-derived chondrogenic cells Fig 24 Response of hESC-derived...
... bioreactors Conflicts of interest The authors have declared that no conflict of interest exists References Becker EW The roles of ATP in the dynamics of the actin filaments of the cytoskeleton ... cytotoxicity assays J Immunol Methods 1983; 65: 55-63 Heng BC, Liu H, Rufaihah AJ, Cao T Human embryonicstem cell (HESC) colonies display a higher degree of spontaneous differentiation when passaged ... Marshall VS, Jones JM Embryonicstem cell lines derived from human blastocysts Science 1998;282(5391):1145-7 Reubinoff BE, Pera MF, Fong CY, Trounson A, Bongso A Embryonicstem cell lines from...
... VEGF-R2-positive cells induced from undifferentiated mouse embryonicstem (ES) cells can differentiate into both VE-cadherin-positive endothelial cells (ECs) and αSMA-positive mural cells (MCs), ... VE-cadherin (+) cells VE-cadherin (-) aSMA (+) cells hES -ECs hES -ECs+MCs aSMA (+) cells hES -MCs Figure cells differentiated from human of the vascular Schematic representation of preparationES cellstransplanted ... Characterization of the transplanted vascular cells derived from human ES cells (HES-3) Characterization of the transplanted vascular cells derived from human ES cells (HES-3) A, Flow cytometric analysis of...
... sources of pluripotent stemcells These include very small embryonic/ epiblast like stem cells, multipotent dental stem cells, pluripotent stemcells from testis and amniotic fluid stemcells In ... contexts with respect to stem cell differentiation: maintenance of pluripotent stemcells and tumorigenesis of cancer stemcells In the first context, inhibition of PTEN, activation of PI3K and Akt/PKB, ... Context-Dependent Regulator ofEmbryonicStemCells 55 Zoltan Simandi and Laszlo Nagy Part Neural and Retinal Differentiation 79 Chapter Pluripotent StemCells as an In Vitro Model of Neuronal Differentiation...
... sources of pluripotent stemcells These include very small embryonic/ epiblast like stem cells, multipotent dental stem cells, pluripotent stemcells from testis and amniotic fluid stemcells In ... contexts with respect to stem cell differentiation: maintenance of pluripotent stemcells and tumorigenesis of cancer stemcells In the first context, inhibition of PTEN, activation of PI3K and Akt/PKB, ... Context-Dependent Regulator ofEmbryonicStemCells 55 Zoltan Simandi and Laszlo Nagy Part Neural and Retinal Differentiation 79 Chapter Pluripotent StemCells as an In Vitro Model of Neuronal Differentiation...
... in mouse -5 embryonicstemcells using DCF-DA (10 M), a fluorescent probe of intracellular H2O2 Production of ROS was -3 detected in the cells exposed to H2O2 10 M for a variety of times (0-60 ... role of dihydrotestosterone in benign prostatic hyperplasia Urology 2003, 61 (Suppl 1), 2-7 16 Chambers I The molecular basis of pluripotency in mouse embryonicstemcells Cloning StemCells ... on mouse embryonicstemcells exposed to H2O2-induced oxidative stress 253 Fig Effect of dihydrotestosterone (DHT) on hydrogen peroxide (H2O2)-induced decrease of DNA synthesis A-D: The cells were...