dh in prevention and treatment of da and bmp
Báo cáo sinh học: " Reference gene selection for quantitative real-time PCR analysis in virus infected cells: SARS corona virus, Yellow fever virus, Human Herpesvirus-6, Camelpox virus and Cytomegalovirus infections" doc
... BestKeeper indices; even actin was included into our gene panel These findings demonstrate the usefulness of analysing a wide variety of reference gene candidates The inconsistent data regarding to ... remained constant, while other genes were varying in expression according to accumulation of infected cells The experimentally obtained data for each virus and each gene were analysed using three ... Corresponding to the recent estimation the SD of the CT value was highest for Act in of viruses, indicating that Act is no reliable reference gene in this setting In contrast, TBP and PPI displayed...
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báo cáo hóa học:" Reference gene selection for quantitative real-time PCR analysis in virus infected cells: SARS corona virus, Yellow fever virus, Human Herpesvirus-6, Camelpox virus and Cytomegalovirus infections" doc
... BestKeeper indices; even actin was included into our gene panel These findings demonstrate the usefulness of analysing a wide variety of reference gene candidates The inconsistent data regarding to ... remained constant, while other genes were varying in expression according to accumulation of infected cells The experimentally obtained data for each virus and each gene were analysed using three ... Corresponding to the recent estimation the SD of the CT value was highest for Act in of viruses, indicating that Act is no reliable reference gene in this setting In contrast, TBP and PPI displayed...
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Báo cáo y học: " Phosphodiesterase type 4 expression and anti-proliferative effects in human pulmonary artery smooth muscle cells" docx
... showing stimulation following treatment of PASMCs with PMA (10-7 M) and TNF-α (10 ng/ml) and its inhibition by cicaprost and forskolin (A-B) Inhibitory effect of roflumilast, both alone (C) and in ... X-100 After fixation in 25% isopropanol and 10% acetic acid for 10 min, gels were stained in 0.25% Coomassie blue for 1–2 h and destained in fixing/destain solution until bands of activity were clearly ... interaction is perhaps not surprising given the critical role of the cAMP-protein kinase A signalling pathway in regulating the expression, activity and intracellular localization of PDE4 isoforms...
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Báo cáo y học: " Ancient, independent evolution and distinct molecular features of the novel human T-lymphotropic virus type 4" pot
... Recently, the C-terminus of Tax1, but not Tax2, has been shown to contain a conserved PDZ binding domain present in cellular proteins involved in signal transduction and induction of IL2-independent ... evolved in Africa and represents a strain circulating within humans living in this geographic region Interestingly, the inferred date of the HTLV-4 ancestor also coincides with the appearance of Homo ... confidence intervals for the origin of HTLV-2 as it migrated out of Africa and Asia and into the Americas via the Bering land bridge and 40,000 – 60,000 ya as confidence intervals for the origin of HTLV-1...
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AC R Sample Task Type 4 Task
... Choose NO MORE THAN THREE WORDS from the passage for each answer. Write your answers in boxes 913 on your answer sheet. Species Size Preferred climate Complementary species Start of active period Number of generations per year French ... Start of active period Number of generations per year French 2.5 cm cool Spanish late spring 1 2 Spanish 1.25 cm 9 10 11 South African ball roller 12 13 ……… ...
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A comparative study of discourse structures and some major linguistic features of international declarations and international conventions on human rights part 4
... and leisure, including reasonable limitation of working hours and periodic holidays with pay Article 25 Everyone has the right to a standard of living adequate for the health and well-being of ... Considering that, in accordance with the principles proclaimed in the Charter of the United Nations, recognition of the inherent dignity and of the equal and inalienable rights of all members of the ... freedom of expression; this right shall include freedom to seek, receive and impart information and ideas of all kinds, regardless of frontiers, either orally, in writing or in print, in the form of...
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Tài liệu E-Human Resource Management 4 pptx
... businesses under preInternet conditions Such individuals often had little understanding of ecommerce, and had difficulty envisioning any positive impact of the Internet on their thriving businesses ... play in the development of organizational learning The moves we have outlined above towards a new balance between innovation, trust, and learning on the one hand and new forms of discipline and ... Learning both at individual and at organizational levels will also have to be of a high order in order to facilitate the necessary continuous improvement and innovation Developing and supporting...
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Báo cáo khoa học: The Saccharomyces cerevisiae orthologue of the human protein phosphatase 4 core regulatory subunit R2 confers resistance to the anticancer drug cisplatin pot
... Resistance to cisplatin sensitivity to oxaliplatin and cisplatin were in the DNA damage and repair pathways However, one strain with a deletion of the protein serine ⁄ threonine phosphatase, Pph3p, ... spotted in that order (from left to right) on plates containing the indicated concentrations of benomyl and grown at 26 °C for days of strains carrying deletions of PPH3, YBL046w ⁄ PSY4 and PSY2 ... the phenotype of S cerevisiae strains carrying a deletion of the YBL046w gene Haploid yeast strains carrying a null allele of YBL046w in AY925 and AY926 backgrounds and diploid strains homozygous...
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Báo cáo khoa học: Mutational analysis of substrate recognition by human arginase type I ) agmatinase activity of the N130D variant pot
... Agmatinase activity of the N130D mutant arginase I Fig Inhibition of wild-type and N130D variants of human arginase I by agmatine (A) and putrescine (B) The arginine concentration was mM and the ... by measuring the production of L-ornithine from L-arginine Initial velocities in the absence and presence of putrescine are expressed as vo and vi, respectively A B Fig Effects of varying concentrations ... specific for arginine over agmatine and, like the nonplant arginases, they are competitively inhibited by NG-hydroxy-l-arginine, an intermediate of NO synthesis [26] On the other hand, in contrast...
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Báo cáo khoa học: Effect of heliquinomycin on the activity of human minichromosome maintenance 4/6/7 helicase pdf
... dominant in the presence of 14 lm of heliquinomycin, and larger complexes were detected in the presence of 43 lm of heliquinomycin Thus, it appears that higher concentrations of heliquinomycin ... heliquinomycin inhibits the helicase activity of MCM4/6/7 by interfering with the interaction of this complex with single-stranded DNA Inhibition of MCM4/6/7 helicase with heliquinomycin A B ... 440 kDa Fig Effect of increasing concentrations of heliquinomycin (HQ) on the formation of the MCM4/6/7 complex The MCM4/6/7 complex was incubated in the presence or absence of heliquinomycin and...
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Báo cáo khoa học: Functional analysis of disease-causing mutations in human UDP-galactose 4-epimerase pot
... of disease Some of the mutants have only small changes in the kinetic parameters In the case of N34S and D103G, disease-causation may be explained by a combination of kinetic impairment and increased ... to 4% of that observed for the wild-type protein and its abundance to 6% [22] Residue 183 forms part of a b-sheet in the interior of the protein (Fig 5), and mutation from leucine to proline is ... during purification Kinetic analysis of disease-causing mutations The steady-state kinetic parameters of each of the nine disease-causing mutant proteins were determined (Table 1) In general, little...
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Báo cáo sinh học: " Purification of infectious human herpesvirus 6A virions and association of host cell proteins" pot
... h incubation, cells were washed and maintained in RPMI containing 10% FCS for 24 h The cells were washed and RPMI containing 2% FCS was added and incubation continued At time points h, 1, 3, and ... labelling The cells were infected for h and maintained in RPMI containing 5% FCS for 21 or 69 h The cells were washed with PBS and incubated for 30 in low-methionine DMEM medium (3.0 µg of methionine/ml) ... silver staining As seen in lanes and in Fig 2A and 2B, a considerable amount of protein containing material from both HHV-6A and mock preparations was detected in the top fractions of the iodixanol...
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Báo cáo sinh học: " The simultaneous presence and expression of human hepatitis C virus (HCV), human herpesvirus-6 (HHV-6), and human immunodeficiency virus-1 (HIV-1) in a single human T-cell" docx
... cultures were incubated overnight post infection and allowed to incubate at 37°C for the remainder of the experiment HHV-6A infected CEM cells (K1) were infected with HCV using our standard infection ... dynamics of AIDS by prolonging the lives of HIV-infected individuals irrespective of infections and other associated problems [4] Our effort is to understand the impact of multiple infections ... infected cells and finally the HCV infected ones died At this point these experiments were terminated Infection of single cells Since PCR analysis showed that all three viruses were present in...
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Báo cáo sinh học: " Thiol-reactive reagents inhibits intracellular trafficking of human papillomavirus type 16 pseudovirions by binding to cysteine residues of major capsid protein L" pot
... containing mM of DTNB, MBTA or MTSET for days grew and were maintained 1 C C C CC C C C C Figure Alignment of L1 amino acid sequences of papillomaviruses Alignment of L1 amino acid sequences of ... concentration of mM 3B) The data indicate that BPEOIA bound to the free thiol of cysteine residue(s) of L1 Binding of BPEOIA to the L1 cysteine residues of 16PV BPEOIA, capable of making a complex ... and enhanced sensitivity to the cellular mechanisms controlling the degradation of foreign proteins Infectivity of the 16PVs composed of mutant L1s having a replacement of cysteine with alanine...
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Báo cáo sinh học: " The E5 protein of the human papillomavirus type 16 down-regulates HLA-I surface expression in calnexin-expressing but not in calnexin-deficient c" pptx
... calnexin-containing but not in calnexin-deficient cells HPV16 E5 decreases HLA-I surface expression in calnexin-containing but not in calnexin-deficient cells CEM-C7 (calnexin) and CEM-NKR (no calnexin) ... transmembrane domain and on the subsequent interaction between HPV16 E5 and calnexin The definitive finding presented here is the existence of a ternary protein complex of HPV16 E5, calnexin, and the heavy ... in the ER/Golgi of the infected cell A direct binding of E5 to the heavy chain of HLA-I seems under the light of our results improbable This is further supported by our findings using calnexin-deficient...
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Báo cáo sinh học: " Human herpesvirus 6A induces apoptosis of primary human fetal astrocytes via both caspase-dependent and -independent pathways" potx
... increased the cytosolic level of AIF in PHFAs, indicating involvement of caspase-independent pathway of apoptosis [29] In addition, NF-κB reportedly induces the expression of c-IAP1, c-IAP2 and ... pro-apoptotic protein Bax during HHV-6A infection, suggesting that Bcl-2 and Bax were involved in the apoptosis of HHV-6A-infected PHFAs Up-regulation of Bax induces the permeabilization of mitochondrial ... staining and flow cytometry Infected and uninfected PHFAs were trypsinized, washed in PBS and incubated with Annexin VFITC and PI solution (Bender MedSystems, Burlingame) in the dark for 15 Samples were...
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Báo cáo sinh học: " Human herpesvirus 8 – A novel human pathogen" potx
... [41] By quantifying the level of viral infection by indirect immunofluorescence assay (IFA), inhibition of infection was determined by comparing the level of infection in cells obtained with HHV-8 ... the low incidence of infection in most populations studied, and because of the lack of known defining features By using a diagnosis of exclusion and the temporal occurrence of symptoms and diagnostic ... greater incidence of CKS but surprisingly, increased cigarette smoking actually lowered the risk [119] The increased prevalence in Sardina of HHV-8 and CKS among family members of KS patients indicates...
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nghiên cứu phát hiện sự gắn chèn gene e6 và e7 của human papilomavirus type 16 vào bộ gene người bằng kỹ thuật multiplex rt-pcr
... base CIN Cervical Intraepithelial Neoplasia: T n thương t bào bi u mô c t cung Cs C ng s CD Ch ng dương cDNA complementary DNA: DNA b sung dATP Deoxyadenosine triphosphate dCTP Deoxycytidine triphosphate ... Ribonucleotide acid NCBI National Center for Biotechnology Information: Trung tâm Qu c gia v thông tin Công ngh Sinh h c ix PCR Polymerase chain reaction: Ph n ng kéo dài chu i PK Phòng khám PK K ... Molecular Biology Labolatories: H i phòng thí nghi m Sinh h c phân t Châu Âu HPV Human Papilomavirus IARC International Agenecy For Research on Cancer IDT Intergrated DNA Technology Kb Kilo base mRNA...
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Nuclear Power Control, Reliability and Human Factors Part 4 docx
... at several industrial companies and Universities in Canada: 1) Engineering Services, Inc (Toronto) (ESI, n.d.), University of Toronto (Department of Mechanical and Industrial Engineering) (RAL, ... viewed as providing a spectrum of capabilities with automated control representing the lowest extreme or baseline of the continuum The incorporation of increasing intelligence and fault tolerance ... Systems 107 system-level information and commands, and spacecraft-level status and demands The effective integration of data and information at each level requires a well-defined functional architecture...
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Báo cáo hóa học: " Purification of infectious human herpesvirus 6A virions and association of host cell proteins" potx
... h incubation, cells were washed and maintained in RPMI containing 10% FCS for 24 h The cells were washed and RPMI containing 2% FCS was added and incubation continued At time points h, 1, 3, and ... labelling The cells were infected for h and maintained in RPMI containing 5% FCS for 21 or 69 h The cells were washed with PBS and incubated for 30 in low-methionine DMEM medium (3.0 µg of methionine/ml) ... silver staining As seen in lanes and in Fig 2A and 2B, a considerable amount of protein containing material from both HHV-6A and mock preparations was detected in the top fractions of the iodixanol...
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