determination of sensitivity of the sequencing method to detect expressing allele of p53
Regulation and functional analysis of the tumor suppressor gene product, p53
... expressed the p53Pro allele However, the expression of p53Pro allele in Chinese healthy heterozygotes (p53Arg/p53Pro) suggests that there is a selection preference on the expression of p53 alleles To ... of p53 codon 72 polymorphism in 46 Singaporean population Determination of sensitivity of sequencing method 48 Expressing allele of p53 in Chinese healthy heterozygotes 49 Expressing allele of ... whereas most of the p53Pro forms carry mutation in the DNA binding domain of p53 So, the data suggest that the expression of p53Arg allele may lead to cancer development in Asians Thus, the expression...
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Báo cáo y học: " Generation and characterization of high affinity human monoclonal antibodies that neutralize staphylococcal enterotoxin B" ppsx
... operator, or the cytokine analyzed, the EC50 of HuMAb154 always remained below ng/mL (Table 2) Crossreactivity of HuMAb-154 to other bacterial toxins To test whether HuMAb-154 crossreacted to other ... and therapeutic countermeasures of SEB exposure Conclusions There are still no readily available therapeutics to counteract the effects of SEB intoxication, whether the exposure is a result of ... models better mimic the progression of the toxicity predicted in humans and may offer a wider window for therapeutic intervention We will therefore explore these models to further test HuMAb154...
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GENERATION AND CHARACTERIZATION OF HUMAN MONOCLONAL ANTIBODIES WITH NEUTRALIZING ACTIVITY FOR DENGUE VIRUS
... of folding back forces the target membrane and viral membrane to bend towards each other for its subsequent fusion The nucleocapsid of the virus particle is then released into the cytoplasm of ... Subsequent fusion of viral and endosomal membranes in acidic conditions lead to the disassembly of the virion and the release of viral RNA into the cytoplasm Viral RNA is then translated into a polyprotein ... antigen They are roughly Y-shaped proteins consisting of identical pairs of heavy (H) and light (L) polypeptide chains forming the two arms of the Y 46 The tips of the two arms of the Y make up the...
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Isolation and characterization of stem cell regulatory genes oct4 and stat3 from the model fish medaka
... factors and roughly half of these genes are expressed in hESCs Thus, the three factors work together, rather than separately, to control whole sets of target genes in ES cells The central factors ... together, these studies demonstrated that expression of 13 Chapter I Introduction oct4 is regulated by the changes of DNA methylation The statue of DNA methylation is related to the changes of ... have the capacity to self renew and they are involved in the generation or regeneration of tissues; on the other hand, they have the potential to differentiate into various types of cells (Blau...
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Tài liệu Báo cáo khoa học: Identification and characterization of the transcription factors involved in T-cell development, t-bet, stat6 and foxp3, within the zebrafish, Danio rerio docx
... unclear whether these cytokines are produced by CD4+CD25+Foxp3+Treg or whether they induce the production of these cytokines from another population of cells [20] To date, our knowledge about the different ... regulator of Th1 differentiation [11] T-bet potentiates the expression of IFN-c, which, in turn, upregulates the inducible chain of the IL-12 receptor (IL-12Rb2) Binding of IL-12 to its receptor ... signalling, together with IL-4 receptor signalling via signal transducer and activator of transcription (STAT6) This, in turn, up-regulates the low-level expression of GATA3, the master regulator of Th2...
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Báo cáo khoa học: Superoxide radical-scavenging effects from polymorphonuclear leukocytes and toxicity in human cell lines of newly synthesized organic selenium compounds potx
... from the cytosol to the cell membrane upon activation To examine the effects of selenoureas and selenoamides on O2– production from PMNs, we investigated the translocation of the molecule to the ... Turuk, Finland) The concentration of the oxidant in the samples was calculated using the standard curve, which was made by adding known concentrations of the authentic H2O2 instead of the samples ... attributed to the activation of a plasma membrane NADPH oxidase The NADPH oxidase multicomponent enzyme system catalyzes the production of O2– A component of NADPH oxidase, p47phox, translocates to the...
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Báo cáo Y học: Purification and characterization of two secreted purple acid phosphatase isozymes from phosphate-starved tomato (Lycopersicon esculentum) cell cultures ppt
... Influence of Pi starvation on growth and secreted AP activity of tomato suspension cells Tomato cells cultured for days in the absence of exogenous Pi had only approximately 40% of the fresh weight of ... shown in parentheses Numbering is relative to the first amino acid of the deduced AP sequence, and colons indicate an amino acid residue identical to that of the respective tomato PAP peptide ... activity The ability of tomato SAP1 and SAP2 to catalyze the peroxidation of luminol was investigated using a chemiluminescence assay In the presence of luminol and H2O2, nM amounts of SAP1 and...
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Báo cáo Y học: Isolation and characterization of MUC15, a novel cell membrane-associated mucin pot
... of MUC15 at the protein level The significance of the potential coexistence of MUC15 splice variants is unclear However, the MUC1/SEC secreted form of MUC1, devoid of the transmembrane and cytoplasmic ... appears to have originated from a common ancestral gene [34] Despite the location of the MUC15 gene close to the cluster of mucin genes, it does not show the characteristics of this group of secreted ... suggested by the staining behaviour of the protein, might explain at least a part of this discrepancy The carbohydrate might thereby constitute up to 67% of the relative molecular mass, although the massive...
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Báo cáo y học: "Genetic characterization of the cell-adapted PanAsia strain of foot-and-mouth disease virus O/Fujian/CHA/5/99 isolated from swine" doc
... Page of 11 countries of the Far East such as Mongolia (2000) and Russia (2000) [3,11,33] The extent to the genetic diversity of these PanAsia virus isolates accumulating over the course of FMD ... which compared to reference strains of PanAsia lineage Downstream of the poly(C) tract, there is a stretch sequence of highly tolerant to changes, containing four PKs in structure of O/Fujian/CHA/5/99 ... outbreaks of FMD caused by FMDV serotype O, occurred in the countries and districts of East Asia (EA) and the Far East [11] The O/ YUN/TAW/97 strain, a member of the Cathay topotype, containing the...
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Báo cáo y học: "Physiological properties of astroglial cell lines derived from mice with high (SAMP8) and low (SAMR1, ICR) levels of endogenous retrovirus" ppsx
... It is, of course, possible that other factors during the development of the cell lines affect their morphology and physiological characteristics The expression of MuLV with regard to the virus ... lines The difference in color of the merge pictures seen for the R1A1 and P8A9 is probably a function of the fact that the concentration of CAgag is higher in the P8 cell lines than in the R1 ... some of which have the capacity to produce intact virions (MuLV)[8] One of the progenitors of the SAM strains, the AKR mouse strain, expresses high levels of the prototype ecotropic endogenous retrovirus,...
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Báo cáo y học: " The establishment and characterization of the first canine hepatocellular carcinoma cell line, which resembles human oncogenic expression patterns" ppsx
... definitive proof of the hepatocytic and neoplastic nature of the cHCC cells Positive staining for hepatocyte marker HepPar1 strongly indicates the hepatocytic origin of the cultured cells [10] The neoplastic ... with the neoplastic nature of the cells [11], and suggests the presence of both fully differentiated hepatocytes and progenitor cells in the cHCC cell culture The hepatocytic nature of the cHCC ... [10] The neoplastic nature of the cells can be deduced from the pleiomorphism of the cells, the number of sometimes bizarre mitotic figures The simultaneous presence of CK7 and HepPar1 positive...
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Báo cáo sinh học: " Establishment of stable Huh-7 cell lines expressing various hepatitis C virus genotype 3a protein: an in-vitro testing system for novel anti-HCV drugs" potx
... genes of the exact sizes These bands were confirmed by sequencing and only the sequence confirmed genes were further used in next experiment leading to the development of expression vectors The ... applied to the transfected cells by growing them in the presence of mg of G418/ml About 80% of cells did not develop resistance to the selecting agent, but in the long run it was possible to identify ... in other regions of the world [7,37] The HCV stable cell lines may be very useful in the study of HCV genomic replication in that part of the world Figure a) Showing the amplified genes of Core...
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Characterization of MP2 cell division and pins function on spindle asymmetry of drosophila central nervous system
... translocates into the nucleus despite the high level of Mira at the cortex (Ikeshima, et al., 1997) Alternatively, the degradation of Mira might be the consequence of release of Pros from the cortex ... mutant, miraRR127, Pros is localized to the basal cortex of the NB and segregated to the GMC normally but then fails to release from the cortex and translocate to the nucleus Molecular analysis showed ... transformation of vMP2 into dMP2 Hence, Numb functions as a determinant to specify the fate of the daughter that inherits the protein The asymmetric segregation of Numb is also required in muscle progenitor...
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Development and characterization of a SARS coronavirus replicon cell line
... remains to be determined 1.5 Life cycle of coronavirus Coronavirus infection starts with the binding of the S protein on the surface of coronavirus binds to the receptor on the surface of human ... cytometry were used to observe the green fluorescence of GFP-BlaR protein expressed from the SARS-CoV replicon They were done at various time points to monitor the presence of the replicon in the ... minutes The contents of the two tubes were then mixed together and left at room temperature for 20 minutes to allow the formation of RNA-Lipofectamine 2000 complex During this time, the original...
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Expression and characterization of FAT1 and atrophin 1 proteins regulating planar cell polarity and MBD1 protein involved in lymphoma
... FAT The answer to the question of how the cells in an epithelium align themselves to the other cells in the tissue lies in the understanding of the interaction of upstream factor Fat with another ... the coordinated organization of cells within the plane of a single layered sheet of cells i.e., the organization of groups of cells in the plane of the epithelium, such that they all orient to ... similar to that with the absence of both This kind of patterning defect was found in the development of R3 photoreceptor in the eye, closure of the last thoracic segment in the abdomen of the Drosophila...
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Isolation and characterization of an extracellular polymer closely related to flocculation of activated sludge
... observed Figure shows the effect of the concentration of the polymer on the flocculation of kaolin clay When the polymer concentration was changed from zero to 12.5 ppm, the extent of flocculation ... measured The activity was expressed as the extent of flocculation calculated by the following formula Extent of flocculation (%) = (A-B)×100/A A: A660 of the total mixed liquor B: A660 of the upper ... acid at 80°C After 16 h, 0.5 ml of distilled water was added and further allowed to stand at 80°C for h The alditol acetates were prepared by the reduction of the hydrolyzed sugars with sodium...
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Preparation and characterization of the PVDF-based composite membrane for direct methanol fuel cells
... adopted to prepare the PVDF composite membrane The structure and the morphology of the PVDF-SPS composite membrane were characterized The proton conductivity and the methanol permeability of the ... the inner part of the membranes As shown in Figure 4e, many big aggregates appear in the middle part of the PVDF-PS membrane The hydrophobic property of PS polymer promoted the formation of these ... and the methanol permeability of the membranes can be calculated from the slopes of the linear curves according to Eq (2) The methanol permeability of the PVDF-SPS membrane was measured to be...
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Design, fabrication, and characterization of a solenoidsystem to generate magnetic field for an ECR proton source
... The use of jacket is to provide a return path for the magnetic field lines and thereby shield the adjacent components The use of electromagnet has wide flexibility of tuning the plasma to get the ... G/A The ampere-turns were determined to produce the maximum possible magnetic field on the axis The size and the power consumption of the solenoid coils were the limiting factors In order to increase ... errors in construction, and the stability of the power supply used to energize it Therefore, after the electromagnets are built, they have to be tested for their performance The magnetic field measurements...
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Tài liệu Báo cáo "Preparation and characterization of nanocomposite TiO2/SnO2 films " pdf
... according to time and temperature of annealing In the other hand, SnO2 is self-doping semiconductor Due to the stoichiometric deviation, some Sn atoms were formed and played a role of the dopant These ... used the photoconductivity measurement The samples were prepared in a shape of photo resistor Contact electrodes were made from SnO2:F The sheet resistance of the contacts is about 10 Ω/□ The ... microscopy (SEM) analyses to identify the structure, and the morphology of the samples Figure shows the XRD result for the TiO2 film prepared from TiCl4 at 400 oC The sharp peaks of the XRD pattern indicate...
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Tài liệu Báo cáo khoa học: Molecular cloning and characterization of soybean protein disulfide isomerase family proteins with nonclassic active center motifs pdf
... neither protein was able to catalyze the oxidation of thiol residues on the synthetic peptide and the oxidative refolding of reduced and denatured RNase A In addition, neither protein reduced the ... in the ER prior to processing in the protein storage vacuoles The synthesis of proglycinin and pro-b-conglycinin was initiated when the seeds achieved a mass of 50 mg (Fig 5A, lanes and 4) The ... the presence of MgCl2 In the presence of EDTA, which releases ribosomes from the rough ER [24], the peaks of GmPDIL-3a and GmPDIL-3b were shifted to the lighter sucrose fractions and therefore had...
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Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam xác định các nguyên tắc biên soạn khảo sát chương trình đào tạo gắn với các giáo trình cụ thể tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra đối với đối tượng giảng viên và đối tượng quản lí điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu mở máy động cơ lồng sóc mở máy động cơ rôto dây quấn các đặc tính của động cơ điện không đồng bộ đặc tuyến hiệu suất h fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến tốc độ rôto n fi p2 đặc tuyến dòng điện stato i1 fi p2 động cơ điện không đồng bộ một pha sự cần thiết phải đầu tư xây dựng nhà máy thông tin liên lạc và các dịch vụ từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008