detector what is it
Báo cáo y học: " GM-CSF and IL-2 as adjuvant enhance the immune effect of protein vaccine against foot-and-mouth disease" pot
... adjuvant It suggested that these cytokines enhanced the cell-mediated immunity, which was consistent with their known biological function Antigen specific delayed-type hypersensitivity response ... GM-CSF with VP1 had a better adjuvant effect than single cytokine It indicated there was synergistic effect between IL-2 and GM-CSF, which was consistent with the previous reports [15,22,23] This ... consistent with the results of ELISA and T cell proliferation It was worth noting that the DTH response level of VP1+CFA group was higher than groups of single cytokine co-expressed or mixed with...
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Báo cáo y học: "Treatment of hypertriglyceridemia and HIV: fenofibrate-induced changes in the expression of chemokine genes in circulating leukocytes" ppsx
... mutated allele (SDF13'A) is associated with a lower incidence of atherosclerosis in HIV-infected patients AIDS 2005, 19:1877-1883 Publish with Bio Med Central and every scientist can read your work ... role in HIV infection because it is the natural ligand for CXCR4 which is used by HIV Ttropic strains to enter into the cells in advanced stages of the disease [10] Additionally, although the interpretation ... P, Piecuch S, Basgoz N, Davis B, Sax P, Stanley T, Wilson PW, D'Agostino RB, Grinspoon S: Metabolic abnormalities and cardiovascular disease risk factors in adults with human immunodeficiency...
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Báo cáo khoa học: Hydrogen independent expression of hupSL genes in Thiocapsa roseopersicina BBS pot
... This This This This work work work work [35] Stratagene Promega This work Stratagene This work [10] [10] [28] [29] [36] GenBank no L05081 This work This work This work This work [37] [15] This ... [NiFe] site in RH [5] The catalytic activity of RH is low, but the activity is insensitive to oxygen [4] It has been purified as a tetramer with an a2b2 structure This tetramer forms a complex with ... activity of HupSL could not be detected in Ni-free conditions; however, the b-galactosidase activities were unchaged (55.6 ± 6.2 Miller units in Ni-free conditions and 57.7 ± 5.6 Miller units...
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expression of recombinant genes in eukaryotic systems
... ensure that it is full length, as oligonucleotide synthesis is performed 3' to 5' and contamination with truncated oligonucleotides will result in poor in vitro transcription yields This method ... been defined, it is advisable to establish several transfection pools in parallel to increase the probability of establishment of a high-producer cell line Primary Selection Selection is usually ... procedure, the MTX concentration is increased in a stepwise fashion, e.g., in fivefold increments During this procedure it is important to monitor production titers at each level of MTX, as these...
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Báo cáo sinh học: "Multicistronic lentiviral vectors containing the FMDV 2A cleavage factor demonstrate robust expression of encoded genes at limiting MOI" potx
... minimizing the risk of insertional mutagenesis In addition to the potential for intracellular mislocalisation of protein, the addition of 2A peptide [[17] additional amino acids in this case) to ... the monocistronic construct It is clear that both bicistronic vectors express eGFP with lower efficiency than the monocistronic one However, whilst MGMT-2A transduced K562 cells exhibited around ... apparent that limiting MOI is desirable in order to minimize the risk of insertional mutagenesis [19-21] Therefore, in order to determine whether the use of multi-cistronic vectors is realistically...
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báo cáo hóa học:" Multicistronic lentiviral vectors containing the FMDV 2A cleavage factor demonstrate robust expression of encoded genes at limiting MOI" ppt
... minimizing the risk of insertional mutagenesis In addition to the potential for intracellular mislocalisation of protein, the addition of 2A peptide [[17] additional amino acids in this case) to ... the monocistronic construct It is clear that both bicistronic vectors express eGFP with lower efficiency than the monocistronic one However, whilst MGMT-2A transduced K562 cells exhibited around ... apparent that limiting MOI is desirable in order to minimize the risk of insertional mutagenesis [19-21] Therefore, in order to determine whether the use of multi-cistronic vectors is realistically...
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Báo cáo y học: " Comparison of the expression of cytokine genes in the bursal tissues of the chickens following challenge with infectious bursal disease viruses of varying virulence" pps
... following infection by the H or Ts strain This is consistent with the fact that IL-10 plays a dual role in infectious diseases [37] and is in agreement with the observation made recently by Abdul-Careem ... expression This is in accordance with the pathology and clinical signs of two distinctly virulent IBDV Infection with vvIBDV results in lymphoid depletion, marked atrophy of the bursal tissues and ... associated with immunization against Marek’s disease in chickens Vaccine 2007, 25:424-432 39 Djeraba A, Bernardet N, Dambrine G, Quere P: Nitric oxide inhibits Marek’s disease virus replication but is...
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Differential expression of cellular genes during a west nile virus infection
... infected individuals develop encephalitis, meningoencephalitis, pan-meningo- polioencephalitis (Omalu et al., 2003) or hepatitis The brainstem, particularly the medulla, is the primary central nervous ... arrays is often of a high dimensionality A 10 array experiment with 15,000 genes will constitute a matrix of 10 x 15,000 To facilitate a visual analysis of the data, a reduction of the dimensionality ... groups from microarray analysis………………… 125 DISCUSSION 5-1 List of differentially regulated genes involved in pathogenesis…… 136 vii List of Figures LIST OF FIGURES 1.0 LITERATURE REVIEW 1-1 The immature...
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Expression of EBV genes in nasopharyngeal carcinoma
... nasopharynx is lined by mucosa that is covered with pseudostratified columnar epithelium and stratified squamous epithelium, and the mucosa is frequently infiltrated by lymphoid tissue It is from this ... associated with EBNA1, it enables the viral persistence of episomes in EBV infected-cells (Kieff & Rickinson, 2001) EBNA1 is the only EBNA-associated with chromosomes during mitosis and is a key mediator ... BRLF1 can disrupt the viral latency in epithelial cells by itself through an epithelial specific mechanism (Zalani et al., 1996) Therefore, if BRLF1 is expressed in NPC tumour cells, it is possible...
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Báo cáo hóa học: "A mutated xylose reductase increases bioethanol production more than a glucose/xylose facilitator in simultaneous fermentation and co-fermentation of wheat straw" docx
... carried out with a WIS content of 7% with a total working weight of 1.4 kg To obtain the initially desired WIS content in the bioreactor, the pretreated, undetoxified slurry was diluted with sterile ... anaerobic growth (Runquist et al 2010a) in synthetic medium In addition, mXR shifted product formation from xylitol to ethanol The current comparison using isogenic S cerevisiae CEN.PK strains ... regeneration (Pitkänen et al 2003) In addition the low glucose concentration in SSCF favors induction of high affinity hexose transporters, which also display high affinity for xylose (Pitkänen et...
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Báo cáo sinh học: "Co-regulation of mouse genes predicts function" pps
... “But what I like about this paper is that it s really rock solid,” says Stuart Kim of the Stanford University Medical Center, USA “This is really believable stuff It is really well grounded in ... their favorite gene(s) and see where it is expressed in 55 tissues It will cost nothing and then you will know where it is expressed strongly You can make sure there are no hidden surprises [in ... of the Hughes dataset is that it has been made openly accessible to the research community [1,7] The additional data with the published article, and the Hughes lab website, provide information...
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Báo cáo lâm nghiệp: "doubling atmospheric CO concentration on 2 matter distribution and CO exchange of 2 yr growth, dry 2 old sweet chestnut trees (Castanea sativa Mill.)" pptx
... -enriched Dry matter partitioning (Table III) Table III shows that the root biomass of -enriched C0 plants was 69% heavier than roots of the control plants The increase was statistically significant ... measurements Discussion and Conclusion Towards the end of the first growth season (4 Sept.), plants grown at 350 ppm were transferred into an airtight assimilation chamber where environmental conditions ... leaf thickness In the C0 chest-enriched nut plants, stomatal density and stomatal index were not significantly different from the control Growth pattern (Table 11)...
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Báo cáo y học: " Genome-wide comparative analysis of the Brassica rapa gene space reveals genome shrinkage and differential loss of duplicated genes after whole genome triplicatio" potx
... density statistics are shown in Table The base composition of Br and At genes is very similar The average length of Br genes (ATG to stop codon) is 73% that of At genes This is consistent with previous ... perform a BLASTP similarity search because it is inherently more sensitive than BLASTN [60] Moreover, the BLASTP hit matrix contains fewer BLAST hits that are due to repetitive nucleotide sequences ... project and supervised its execution Additional data files The following additional data are available with the online version of this paper: Tables S1, S2, S3, S4, S5 and S6 (Additional file 1);...
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báo cáo khoa học: " Molecular characterisation and genetic mapping of candidate genes for qualitative disease resistance in perennial ryegrass (Lolium perenne L.)" doc
... interactions, similar qualitative and quantitative genetic mechanisms are likely to contribute to disease resistance in perennial ryegrass In order to test this hypothesis, a broad survey based ... linkage disequilibrium in 11 expressed resistance genes in Lolium perenne BMC Plant Biol 2007, 7:43 Faris JD, Li WL, Liu DJ, Chen PD, Gill BS: Candidate gene analysis of quantitative disease resistance ... grisea), dollar spot (Sclerotinia homoeocarpa) and brown patch (Rhizoctonia solani) reduce turf quality [9] The development of cultivars resistant to each of these diseases is currently recognised...
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báo cáo khoa học: " An optimized grapevine RNA isolation procedure and statistical determination of reference genes for real-time RT-PCR during berry development" docx
... preveraison pericarp; 3, veraison pericarp; 4, post-veraison pericarp; 5–6, pre-veraison seed; 7, veraison seed; 8, post-veraison seed; 9, root; 10, pre-anthesis flower; 11, post-anthesis flower ... initiative The authors also wish to thank the Province of British Columbia and the BC wine industry for their continued support of research activities in the University of British Columbia Wine Research ... on the lists in each analysis method, but given that each method resulted in different units, we recognized the need to devise a scheme to standardize the units while maintaining the distribution...
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Cloning, characterisation and functional analysis of horseshoe crab c reactive proteins
... aeruginosa is a leading causative agent (Wenzel et al, 1995) P aeruginosa is a ubiquitous GNB noted for its environmental versatility and its resistance to a range of antibiotics The bacterium is capable ... (Clarke, 1990) Despite its versatility, P aeruginosa is an opportunistic pathogen that only causes clinical manifestations of disease in susceptible hosts (Clarke, 1990) Within a clinical environment, ... possibly limit the development of sepsis Several lines of evidence support this hypothesis with regards to human CRPs In vitro, human CRP (hCRP) binds phosphorylcholine (PC) and its associated...
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Báo cáo y học: "otifs and cis-regulatory modules mediating the expression of genes co-expressed in presynaptic neurons" ppsx
... Elnitski L, Hardison RC, Li J, Yang S, Kolbe D, Eswara P, O'Connor MJ, Schwartz S, Miller W, Chiaromonte F: Distinguishing regulatory DNA from neutral sites Genome Res 2003, 13:64-72 Dermitzakis ... extent, a clue as to tissue/cell type specificity Overall, based on 26 tested MCEs, we were able to predict neuronal-specific expression with roughly 75% sensitivity and 63% specificity Although we ... supplemented with μg/ μl proteinase K (Sigma-Aldrich, St Louis, MO, USA) DNA was extracted using standard phenol-chloroform procedures, precipitated with ethanol, and dissolved in 10 mM Tris/10 mM...
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Tài liệu Báo cáo khoa học: Molecular characterization, phylogenetic relationships, and developmental expression patterns of prion genes in zebrafish (Danio rerio) doc
... by the neighborjoining algorithm based on the number of substitutions per site (Poisson correction distance method, pair-wise-deletion option for gap sites) Reliability of the neighbor-joining ... ctg23943 with prp2 transcript indicated that this gene consisted of at least two exons with the coding sequence contained within exon A coding sequence of 1701 bp, from an ATG codon at position 71 ... duplicated copies of this gene were detected in the fish genome [13] Although Sho is highly conserved from fish to mammals, it has little overall similarity to human PrPC [12] In addition, none of the...
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Tài liệu Báo cáo khoa học: Cloning, characterization and expression analysis of interleukin-10 from the common carp, Cyprinus carpio L. docx
... torafugu and human The x-axis denotes the residue position and the y-axis represents hydrophobicity The hydrophobicity analysis was carried out according to the Kyte and Doolittle method [22] using ... polyadenylation signal in bold italics The stop codon is represented with an asterisk Ó FEBS 2003 Cloning and analysis of IL-10 in fish (Eur J Biochem 270) 4649 1640 supplemented with 10% carp serum and ... sequence to its carp counterpart These comparisons suggest that carp IL-10 shares many characteristics of IL-10 with its family members The general IL-10 signature sequence comprises a 21 amino...
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Tài liệu Báo cáo Y học: Purification, characterization, cloning, and expression of the chicken liver ecto-ATP-diphosphohydrolase pot
... ATPase and ADPase activities were inhibited by 10 mM azide, but ADPase activity was more sensitive to azide inhibition than ATP hydrolysis and inhibition in DISCUSSION The existence of a cell surface ... activity of the purified chicken liver ecto-ATPDase was more sensitive to azide inhibition than its ATPase activity at either pH 7.4 or pH 6.4 (Fig 3) At pH 7.4, mM azide inhibited ADP hydrolysis ... cell membrane, is activated by detergents while unaffected by ConA, indicates that its activity is not dependent on its oligomerization status While it is likely that the enzyme is fully active...
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