culture systems low oxygen culture
Báo cáo y học: "Human meniscus cells express hypoxia inducible factor-1α and increased SOX9 in response to low oxygen tension in cell aggregate culture" ppsx
... only 7-fold (P < 0.05; Figure 2d) in aggregate cultures in 5% oxygen The type II collagen response to lowered oxygen was thus greater in the cells cultured from the outer meniscus The cells from ... aggregate cultures of articular chondrocytes (n = 3) in 5% oxygen versus 20% oxygen (b) Collagen type II alpha (COL2A1) gene expression in aggregate cultures of articular chondrocytes (n = 3) in 5% oxygen ... conical culture tube The cell aggregates were cultured for 14 days in a humidified atmosphere under conditions of normoxia (95% air and 5% carbon dioxide [20% oxygen] ) or hypoxia (5% oxygen, ...
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Báo cáo khoa học: "The origin and interpretation of hyperlactataemia during low oxygen delivery states" pps
... lactate:pyruvate ratio in association with acidosis, low oxygen delivery and hyperlactataemia during CPB would be conclusive evidence that inadequate oxygen delivery and anaerobic mechanisms are predominating ... significance of raised serum lactate concentrations It eloquently demonstrates the association of reduced oxygen delivery in the development of hyperlactataemia during CPB and illustrates the association ... Further insight is gained into the significance of monitoring serum lactate as a means of guiding oxygen delivery during CPB Future studies may definitively identify ‘type A’ lactic acidosis during...
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Embryo Culture Systems doc
... page) Embryo Culture Systems 249 Therefore, it is important to consider the ‘ culture environment’’ when attempting to improve embryo culture systems (Fig 5) Oxygen The concentration of oxygen in ... viability of cultured embryos from several species after transfer to recipients Embryo Culture Systems 237 (37,49,102,136,137) as well as increasing embryo development in culture In the mouse, culture ... embryo have demonstrated that culture at a reduced oxygen concentration results in enhanced development in vitro (263) In the mouse, culture at oxygen concentrations as low as Figure (figure on facing...
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Báo cáo sinh học: "Apical polarity in three-dimensional culture systems: where to now" pptx
... vivo state and allow us to manipulate culture conditions and functions In addition, they could provide keys to the information encoded in the tissue architecture Many of the 3D culture studies ... basement membrane (BM) is in contact with myoepithelial cells (d) S1 cultured cells form a single layered acinus-like structure in 3D culture with apico-basal polarity despite the lack of the myoepithelial ... least we still have not found a way of making all the relevant cells survive in culture As an alternative to primary cultures, cell lines have been developed from both normal and tumor tissues Where...
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báo cáo khoa học: " Characterization and isolation of a T-DNA tagged banana promoter active during in vitro culture and low temperature stress" ppt
... sequences appeared lower than that of the positive control maize ubiquitin promoter (Figure 5C) No background activity was detected in untransformed control cultures (Figure 5D) or cultures transformed ... that in cultures transformed with the positive control ubiquitin promoter (Figure 5C) No staining occurred in untransformed control cultures (Figure 5D) or pCAMBIA-1391Z transformed cultures ... after transformation, differentiating cultures were subcultured on REG medium (MS medium, μM benzyladenine and μM indole acetic acid) for plant regeneration All cultures were kept at 26 ± 2°C in...
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Establishment of autologous culture systems for human embryonic stem cells
... that hESCs cultured on H9 ebF expressed higher level of hESCs-specific markers than H9 dF supported hESCs The degree of differentiation in routine hESCs culture is lower in H9 ebF culture system ... 62 3.3.7 Undifferentiated states analysis of H9 hESCs cultured in various systems 64 3.3.7.1 Immunofluorescence staining and flow cytometry analysis for Oct4 and SSEA-3/4 expression ... Characterization and comparison of H9 hESCs cultured on autologous H9-F, MEF and feeder-free MatrigelTM 55 3.3.1 Cultivation of H9 hESCs in various culture systems 55 3.3.2 Cryopreservation assay...
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High density cell culture ofrhodotorula glutinis using oxygen enriched air
... ensure adequate supply of oxygen, oxygen- enriched air (40% oxygen + 60% air) was used in the fed-batch culture (Fig 2) There was no oxygen limitation throughout the culture Final concentration ... continuous culture (60%) The reason for the low lipid content in high density culture is not clear at present It may be, however, caused by high concentration of salts added during the course of culture, ... the specific growth rate was slowed down to zero R_ $1utinis did not produce any fermentative by-products even under oxygen- limiting condition Another fed-batch culture was carried out to produce...
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The influence of organisational culture on performance measurement systems in libyan higher education
... organisational culture types These are: Hierarchy Culture, Clan Culture, Adhocracy Culture and Market Culture 1.6 The Need for the Research The most important justification for a study on organisational culture ... of organisational culture is to give an overview of the concept of organisational culture and its four types (Hierarchy culture, Clan culture, Market culture and Adhocracy culture) as independent ... creating an organisation’s culture Several types of organisational culture can be described, namely bureaucratic culture, clan culture, entrepreneurial culture, and market culture (Hellriegel et...
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creating performance based culture trough performance management systems
... Cash Flow Profits Build momentum Conquer ‘Change’ mgmt Talent Mgmt Top Talent focus Reduce Fat Innovation/R&D Sales, Sales, Sales Continuity Early wins Increase attrition Everyone Happy Slow Down ... Appraisals Mgmt/HR • • • • • • • • • Flexible or Control? MBO or Character? Best Practice or Fit Culture? Backward or Forward? Quarterly Results or Long-term? No $$ to differentiate* Perfect System, ... to Improve Controllable Achievements Summary… What went Wrong? Wrong Business Model – blame PM Systems Having a good PM System cannot correct a bad business model Focusing on the Process (Best...
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Cambridge.University.Press.The.Crisis.of.Literature.in.the.1790s.Print.Culture.and.the.Public.Sphere.Nov.1999.pdf
... and informal associations and practices which helped to extend the privileges of print culture amongst the lower orders The provision made in the Pitt government’s 1789 bill to increase the stamp ... EighteenthCentury Studies Group at the University of York where I wrote this, and in the Politics of Print Culture MA in the Department of English at Simon Fraser University where I revised it for publication ... and audiences.5 Offering a similar argument for a more socially grounded exploration of literary culture, Robert Darnton rejects ‘the great-man, great-book view of literary history’ as a ‘mystification’...
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Cambridge.University.Press.War.Land.on.the.Eastern.Front.Culture.National.Identity.and.German.Occupation.in.World.War.I.May.2000.pdf
... Mellon Fellowships in the Humanities, the William Penn Fellowship of the University of Pennsylvania, the DAAD-German Academic Exchange Fellowship, and the Title VIII Postdoctoral Research Fellowship ... explain this phenomenon, speaking of ‘ culture layers,’’ ‘ culture ground,’’ and ‘ culture earth.’’ The ground’s speciWc nature demonstrated for them a shallowness of modern history Clamorous prehistory ... from East Prussia by shallow, Xat lowland, with marshy woods and crossed by many rivers Rippled lines of hills marked the coast of the Baltic waters and yielded to a slowly rising east of hilly...
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Báo cáo y học: "Parvovirus B19 Genotype Specific Amino Acid Substitution in NS1 Reduces the Protein’s Cytotoxicity in Culture"
... cells were stored at 37°C in 5% CO2 Transduction efficiency was established using flow cytometry as described below Flow Cytometry HepG2 cells were collected by trypsinization (0.5% Trypsin EDTA (Gibco®) ... Transduction of HepG2 Cells HepG2 cells were seeded in culture flasks and grown overnight in supplemented media as described above The following day the media was removed, cells washed once with ... mechanism of B19 NS1-induced liver cytotoxicity MATERIALS AND METHODS Cell Culture Spodoptera frugiperda-derived SF9 cells were cultured in spinner flasks using BioWhittaker® Insect-XPress cell media...
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Corporate Values and Culture
... ticket out of the company Leadership is, to live perfection Corporate Culture and Values c o l o r w o r k s f o r y o u It is our culture to AGREE on common goals rather then DEFINING ways how to ... that sometimes we have to go slow to go fast We know that it is useless to start working on technical matters if emotional conflicts remain unsolved Corporate Culture and Values c o l o r w ... and fair play is the way how we deal with each other c o l o r w o r k s f o r y o u Corporate Culture and Values The team expects from each of us to contribute Entrapreneurial behaviour characterizes...
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Equivalence in the translation of vietnamese cultural words in the book “wandering through vietnamese culture” by huu ngoc
... words In his classification of culture words, Newmark (1998) concluded five major categories of culture words including ecology, material culture (artifacts), social culture – work and leisure, ... categories as follows: (1) Ecology Animals, plants, local winds, mountains, plains, ice, etc (2) Material culture (artifacts) Food, clothes, housing, transport and communications (3) Social culture – ... frequency of material culture, and to be more specific, food is the highest as compared to other types The ratio among them can be illustrated in the chart as follows: Culture- related words Quantity...
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Some aspects of American culture and society in the twentieth and twenty-first centuries through a number of selected short literary works
... distinguished culture With its origin in water-rice agriculture, the culture of Vietnam is often regarded as community-based culture whereas the American tend to develop their individualistic culture ... and etc make up a culture Culture and society are closely related We not have two different societies with exactly the same culture or one society with completely different culture Let consider ... of American culture and society in the twentieth and twenty-first centuries through literature in the next chapter would be more precise II.1.1 Culture and society For many people, culture is...
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The relationship between an organization’s structure and culture and the effects on business performance
... effectively Organizational culture is also remarkable As the same as other concepts, there are many way to define organizational culture One of the definition is described as follow: Culture (in the sense ... identify its culture Thus, culture in each organization is unique For example: if relationship of customers is cared of the culture, this distinction will be adopted by individuals However, following ... four main types of organizational culture: power, role, task, and person culture. 6 Within power culture, power is central by judgment of key individual In role culture, individuals pay attention...
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The culture of the Renaissance
... murdered Hamlet was waiting for a reaction from Claudius that would prove the ghost’s message Following the King’s outburst after watching the performance, Hamlet confronted his mother and began ... hire and salary, not revenge He took my father grossly, full of bread, With all his crimes broad blown, as flush as May; And how his audit stands who knows save heaven? But in our circumstance and ... to drink from the poisoned cup She falters away and the fencing bout continued Laertes struck a blow against Hamlet with the tip of his poisoned sword, but the duel continued and the swords got...
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MEASURING SAFETY CULTURE IN THE AUSTRALIAN REGIONAL AIRLINE INDUSTRY: THE DEVELOPMENT OF THE AIRLINE SAFETY CULTURE INDEX
... Phương Phùng Thị Vân Báo cáo thực tập III CÁC HỌC THUYẾT VỀ TẠO ĐỘNG LỰC Hệ thống nhu cầu Maslow Theo Maslow, nhu cầu chia thành loại thep mức độ từ thấp đến cao sau: Nhu cầu sinh lý Nhu cầu an toàn ... 41 III CÁC HỌC THUYẾT VỀ TẠO ĐỘNG LỰC - 42 Hệ thống nhu cầu Masslow - 42 Hệ thống tăng cường kỳ vọng B.F Skinner 42...
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Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam xác định các nguyên tắc biên soạn khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo gắn với các giáo trình cụ thể xác định thời lượng học về mặt lí thuyết và thực tế tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra đối với đối tượng giảng viên và đối tượng quản lí khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam nội dung cụ thể cho từng kĩ năng ở từng cấp độ phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ rôto dây quấn các đặc tính của động cơ điện không đồng bộ hệ số công suất cosp fi p2 đặc tuyến hiệu suất h fi p2 đặc tuyến tốc độ rôto n fi p2 đặc tuyến dòng điện stato i1 fi p2 sự cần thiết phải đầu tư xây dựng nhà máy phần 3 giới thiệu nguyên liệu chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008 chỉ tiêu chất lượng 9 tr 25