bovine caprine ovine and porcine preimplantation embryos
Báo cáo khoa học: "Sero-survey on Aino, Akabane, Chuzan, bovine ephemeral fever and Japanese encephalitis virus of cattle and swine in Korea" pptx
... 41 631-721 ,21 ,2791 )oykoT( Q htlaeH minA tsnI ltaN stelgip nrobllits fo niarb eht morf suriv AND gnitanitulggameh a dna suriv sitilahpecne esenapaJ fo noitalosI Y ikasijuF ,T iromiguS ,Y aruiM...
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Báo cáo lâm nghiệp: "Micropropagation of hybrid walnut trees (Juglans nigra x Juglans regia) through culture and multiplication of embryos" ppt
... great variability in their bud-cluster development and shoot elongation After and transfers, we obtained a normal distribution of clones (Fig 1A and iB) Ranking of some clones changed during this ... embryonic axis and seedling shoot tip culture of Juglans nigra L Plant Cell Tissue Cult 6, 189-197 Jay-Allemand C & Cornu D (1986) Culture in vitro d’embryons isol6s de noyer com- Jay-Allemand C., Cornu ... Discussion and Conclusion The studies have shown that the micropropagation of juvenile walnut depends upon many factors At a general level, characteristics of the medium are im- cules and variability...
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Báo cáo thú y: "Spatial patterns of Bovine Corona Virus and Bovine Respiratory Syncytial Virus in the Swedish beef cattle population" pdf
... seropositivity to bovine coronavirus and bovine respiratory syncytial virus indairy herds Vet Rec in press 26 Beaudeau F, Ohlson A, Emanuelson U: Associations between Bovine Coronavirus and Bovine Respiratory ... Beaudeau et al., Spatial patterns of Bovine Corona Virus and Bovine Respiratory Syncytial Virus in the Swedish beef cattle population Acta Veterinaria Scandinavica 2010, 52:33 ... Prevalence of antibodies to bovine respiratory viruses in cattle infected with bovine immunodeficiency virus Vet Rec 2008, 162:660-661 18 Anonymous: Surveillance and control programmes Sweden...
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Báo cáo khoa học: Signaling pathways and preimplantation development of mammalian embryos potx
... including c-fos, c-jun, c-haras and c-myc, have been studied in bovine preimplantation blastocysts [79,80] The c-fos, c-jun and c-ha-ras transcripts, as well as c-Fos, c-Jun and c-Myc proteins have been ... -5a, -5b, -6, -7a, -7b, -10b and -11 have been described [7,8,34] Transcripts of Wnt3a, -6, -7b, -9a and -10b have been detected in blastocysts, and Wnt1 and -4, Sfrp1 and Dkk1 are highly expressed ... kinase)-1, -2, -5, and MAPK ⁄ ERK1 are detected in unfertilized eggs and blastocysts Transcripts and the protein localization of p38-regulated and -activated kinase, p38 MAPK, MK2 and hsp25 have...
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Báo cáo khoa học: " Effect of IP3 and ryanodine treatments on the development of bovine parthenogenetic and reconstructed embryos" ppsx
... Morrey, J and Reed, W A Development of nuclear transfer and Effect of IP3 and ryanodine treatments on the development of bovine parthenogenetic and reconstructed embryos parthenogenetic rabbit embryos ... metaphase II bovine oocytes, and 2) to determine whether IP3 and ryanodine microinjection or electroporation followed by 6-DMAP can lead to the development of bovine reconstructed embryos derived ... nuclear transfer Materials and Methods In vitro maturation The bovine oocytes used in this study were obtained from bovine ovaries collected at a local slaughterhouse and transported at room temperature...
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Báo cáo khoa học: "Effects of Protein Source and Energy Substrates on the In Vitro Development of Bovine Embryos in a Two-step Culture System" ppsx
... the in vitro development of bovine embryos, and to examine the effects of glucose, phosphate and citrate upon the developmental frequency of bovine embryos Materials and Methods In vitr o m a tu ... 12 Lee, E S and Fukui Y Synergistic effect of alanine and glycine on bovine embryos cultured in a chemically defined medium and amino acid uptake by in vitro produced bovine morulae and blastocysts ... H J and Barton, A M Pyruvate and glucose uptake by mouse ova and preimplantation embryos J Reprod Fertil 1984, 72, 9-13 14 Liu, Z and Foote, R H Effects of amino acids and α-amanitin on bovine...
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Báo cáo sinh học: " The incidence of polyploidy and mixoploidy in early bovine embryos derived from in vitro fertilization" ppt
... chromosome anomalies found in embryos (Frazer et al, 1976; Maudlin and Frazer, 1977, 1978; Iwasaki and Nakahara, 1990a) The cytogenetic analysis of bovine embryos cultured in vivo and in vitro has revealed ... occasionally in bovine blastocysts, which may suggest that embryos with such cells are eliminated (Iwasaki and Nakahara, 1990a; Kawarsky et al, 1994, 1996) In the present study early bovine embryos (two ... in early bovine embryos derived from in vitro fertilization Gamete Res 22, 83-91 Iwasaki S, Kono T, Fukatsu H, Nakahara T (1989b) Production of bovine tetraploid embryos electrofusion and their...
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Khảo sát một số chỉ tiêu lâm sàng huyết học và khả năng sinh sản của đàn nái sinh sản sau khi mắc hội chứng rối loạn hô hấp và sinh sản (porcine reproductive and respiratory syndrome)
... h c nông nghi p…………… 71 iv DANH M C CÁC CH VI T T T PRRS : Porcine Reproductive and Respiratory Syndrome PRRSV : Porcine Reproductive and Respiratory Syndrome Vi rút RT-PCR : Real tinme - polycine ... p…………… 66 vii M ð U 1.1 Tính c p thi t c a ñ tài H i ch ng r i lo n hô h p sinh s n l n (Porcine Reproductive and Respiratory Syndrome – PRRS) hay g i b nh “Tai xanh”, b nh truy n nhi m c p tính ... huy t h c kh sinh s n c a ñàn nái sinh s n sau m c H i ch ng r i lo n hô h p sinh s n (Porcine Reproductive and Respiratory Syndrome) t i m t s trang tr i vùng ph c n Hà N i” 1.2 M c ñích yêu c...
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Tài liệu TIỂU LUẬN:CHẨN ĐOÁN VIRUS PRRS (Porcine Reproductive and Respiratory Syndrome) BẰNG KỸ THUẬT ELISA docx
... chứng rối loạn sinh sản hô hấp lợn, virus PRRS Hội chứng rối loạn hô hấp sinh sản lợn (Porcine Reproductive and Respiratory Syndrome) bệnh virus, gây thiệt hại lớn kinh tế phát Mỹ vào năm 1987 ... KN chuẩn - Thêm dung dịch protein không tương tác (non-interacting protein) albumin huyết bê (bovine serum albumin) hay casein vào tất mẫu (kể mẫu chuẩn) Bước gọi "blockin" protein huyết có ... (có nồng độ thấp) phải cạnh tranh với protein khác huyết gắn kết với bề mặt đĩa Sandwich ELISA hạn chế nhược điểm Sandwich ELISA: -Phương pháp sử dụng để phát KN mẫu nghiên cứu bao gồm bước sau...
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Tài liệu Báo cáo khoa học: Preliminary molecular characterization and crystallization of mitochondrial respiratory complex II from porcine heart ppt
... sequence of known bovine heart mitochondrial respiratory complex II, we could still identify the signal peptides for porcine CybL and CybS as follows: MAALLLRHVGRHCLRA HLSPQLCIRNAVP (CybL) and MAVLWRLSAACGPRGGGALVLRT ... dyed with Coomassie blue G250, and the four bands corresponding to subunits of porcine complex II were cut out and N-terminal sequenced by the Edman degradation method and amino acid analyzer in ... mature protein sequences of each porcine complex II subunit were obtained, and flavoprotein, iron-sulfur protein, CybL and CybS were shown to contain 622, 252, 140 and 103 residues, respectively...
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Tài liệu Báo cáo Y học: Proteolysis of bovine b-lactoglobulin during thermal treatment in subdenaturing conditions highlights some structural features of the temperature-modified protein and yields fragments with low immunoreactivity pptx
... fragments CP and 2, as well as fragments TP and 2, were further purified by RP-HPLC and SE-HPLC in the same conditions and with the same equipment used for analysis of the whole proteolyzate, and these ... monomeric bovine beta-lactoglobulin FEBS Lett 381, 237–243 Uhrinova, S., Uhrin, D., Denton, H., Smith, M., Sawyer, L & Barlow, P.N (1998) Complete assignment of 1H, 13C and 15N chemical shifts for bovine ... trypsin; C, chymotrypsin) and after their elution order from a Superdex Peptide column (hence, the P in their names), and correspond to the peaks labeled CP1 and CP2 (or TP1 and TP2) in the chromatograms...
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Identification of serum proteomic biomarkers for early porcine reproductive and respiratory syndrome (PRRS) infection pptx
... = pH4, F5 = pH3, and F6 = organic solvent The fractions F1, F4, and F6 were analyzed on the three surfaces CM10, IMAC30, and H50 at both low and high focus masses Fractions F2 and F3 were excluded ... intensities and low coefficient of variation (CV) values (< 30%) in both the discovery and validation phases Exceptions were fraction F1 on IMAC30 (analyzed at high focus mass) and H50 (both low and ... with the highest sensitivities (77% and 64.5%) and specificities (73% and 69.7%) were found on CM10 at low focus mass with the 14 discriminatory peaks of F1 and the discriminatory peaks of F6,...
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Cytoskeleton reorganization mediates alpha beta integrin-associated actions of laminin on proliferation and survival, but not on steroidogenesis of ovine granulosa cells pdf
... with bicarbonate, penicillin/streptomycin, bovine serum albumin (BSA tissue culture grade) used for culture medium, transferrin, selenium, bovine insulin, androstenedione, LN from EHS tumor (mainly ... pg/well) and the intra- and inter-assay coefficients of variation were less than 7% and 9% respectively The progesterone detection limit was 12 pg/tube (60 pg/ well), and the intra- and inter-assay ... conceived the study, participated in its design and coordination, statistical analysis and interpretation of data and drafted the manuscript FLB, SF and DM read and approved the final manuscript CP died...
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Báo cáo khoa học: Recombinant bovine zona pellucida glycoproteins ZP3 and ZP4 coexpressed in Sf9 cells form a sperm-binding active hetero-complex ppt
... heterospecific bovine ⁄ porcine rZP3 ⁄ rZP4 mixtures Both porcine rZP3 ⁄ bovine rZP4 and bovine rZP3 ⁄ porcine rZP4 inhibited bovine sperm–ZP binding at a level similar to that of the bovine ⁄ bovine ... Recombinant bovine zona pellucida glycoproteins S Kanai et al lectin blot analyses indicated that the major N-linked chains of bovine and porcine rZPGs are similar Thus, bovine and porcine rZPGs ... When bovine rZP4 whose N-terminal His-tag was changed to FLAG-tag (rZP4FLAG) and porcine rZP3 were coexpressed and subjected to the immunoprecipitation using anti-FLAG gels, porcine rZP3 and bovine...
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Báo cáo khoa học: Assessment of porcine and human 16-ene-synthase, a third activity of P450c17, in the formation of an androstenol precursor doc
... transfected with P450c17 and cyt b5 3H-Labeled preg, 17a-OHpreg, and DHEA, used as standards, showed elution peaks at 4.70, 2.30 and 2.50 min, respectively (Fig 2) In both porcine and human assays using ... by human and porcine P450c17, with and without cyt b5 Using porcine and human P450c17 expressed in HEK293 cells in culture, we compared the formation of Ó FEBS 2003 Biosynthesis of 5,16-androstadien-3b-ol ... that human and porcine P450c17 have similar catalytic activities and that, in both species, P450c17 is involved in the biosynthesis of androstadienol Effect of cyt b5 on DHEA and androstadienol...
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Báo cáo Y học: Cloning and expression of sterol D14-reductase from bovine liver potx
... of the bovine cDNA encoding sterol D14-reductase BLASTN search of the bovine EST database was performed to identify bovine cDNA clones homologous to human SR-1 cDNA [27] The putative bovine cDNA ... from bovine liver ER with a high degree of identity with both human SR-1 and human LBR A cDNA encoding this protein was identi®ed by bovine expressed sequence tag (EST) analysis, cloned, and expressed ... a polyclonal antibody raised against the bovine liver D14-SR The antibody recognized a single band of 38 kDa both in D14-SR transfected cells and in bovine liver microsomes (Fig 3) No protein...
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Báo cáo Y học: Interaction of bovine coagulation factor X and its glutamic-acid-containing fragments with phospholipid membranes A surface plasmon resonance study pdf
... standard Fmoc chemistry The fragments Gla–EGFN (residues 1–86) Gla– EGFNC (residues 1–140, 154–183) were generated by digestion of bovine factor X with trypsin [22] Bovine factor X, factor Xa and ... and/ or the epidermal growth factor-like domains J Biol Chem 266, 2444–2452 Ó FEBS 2002 23 Nelsestuen, G.L & Suttie, J.W (1972) Mode of action of vitamin K and calcium binding properties of bovine ... titration curves of factor X and Gla– EGFN binding indicate cooperative binding (Fig 1) Halfmaximal binding occurred at a calcium concentration of 1.5 and 1.4 mM for factor X and Gla–EGFN, respectively,...
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Báo cáo khoa học: Differential tissue-specific distribution of transcripts for the duplicated fatty acid-binding protein 10 (fabp10) genes in embryos, larvae and adult zebrafish (Danio rerio) docx
... intestine and testis of adult zebrafish and only in the liver of zebrafish embryos and larvae [6] By contrast, zebrafish fabp10b transcripts were only detected in the olfactory vesicles of embryos and ... in muscle and heart were 6–24 times higher than in brain, eye, gills, testis, skin, kidney and swimbladder, and 320–650 times higher than in liver, intestine and ovary Both RT-PCR and RT-qPCR ... highest sequence identity and similarity (48% and 74%, respectively) with shark FABP10, and the next highest sequence identity and similarity with zebrafish Fabp10a (46% and 75%, respectively) (Fig...
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Báo cáo khoa học: Hydrolysis of acetylthiocoline, o-nitroacetanilide and o-nitrotrifluoroacetanilide by fetal bovine serum acetylcholinesterase doc
... Montenegro et al Fig Esterase and amidase activities of purified fetal bovine serum AChE are due to AChE (A) Effect of AChE inhibitors on esterase and amidase activities of fetal bovine serum AChE Inhibition ... Enzyme Substrate Km (mM) kcat (min)1) kcat ⁄ Km (M)1Æmin)1) Reference Fetal bovine serum AChE Fetal bovine serum AChE Fetal bovine serum AChE Human AChE Human BuChE Human BuChE Human BuChE ATCh F-ONA ... accessibility and binding of F-ONA to the acyl-binding site The calculated Km value for fetal bovine serum AChE with ATCh as substrate (0.23 mm) is similar to those reported for AChE of bovine (0.22 mm) and...
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Tìm thêm: xác định các mục tiêu của chương trình khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản khảo sát chương trình đào tạo gắn với các giáo trình cụ thể xác định thời lượng học về mặt lí thuyết và thực tế tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra đối với đối tượng giảng viên và đối tượng quản lí điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ rôto dây quấn đặc tuyến tốc độ rôto n fi p2 đặc tuyến dòng điện stato i1 fi p2 động cơ điện không đồng bộ một pha thông tin liên lạc và các dịch vụ phần 3 giới thiệu nguyên liệu từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng 9 tr 25