analysis of transcriptional regulation in bone cells
Tài liệu Báo cáo khoa học: Modulation of cyclin D1 and early growth response factor-1 gene expression in interleukin-1b-treated rat smooth muscle cells by n-6 and n-3 polyunsaturated fatty acids pdf
... role in the regulation of cell proliferation, including SMC, by inhibiting signalling molecules [45–47] Caveolin-3 is the main isoform in muscle cells, including SMC [30] Caveolin-1 and caveolin-3 ... for the regulation of egr-1 gene expression in the chromatin context The drastic down -regulation of Egr-1 mRNA levels, which is observed in n-3 treated cells, also indicates that other binding elements ... concentrations of the caveolin-1 and caveolin-3 proteins in the caveolae of cells stimulated with IL-1b in order to determine whether caveolin synthesis is also modulated by PUFA incorporation...
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Báo cáo khoa học: Efficient inhibition of b-secretase gene expression in HEK293 cells by tRNAVal-driven and CTE-helicase associated hammerhead ribozymes doc
... preclearing process of the cell protein extract (100 lg of protein in 500 lL of extract) was carried out as described above Immunoprecipitation and determination of the intracellular level of Ab40 ... level of BACE protein, and in consequence, to reduce the amount of Ab peptide released in neuronal cells We asked the question whether we can inhibit expression of the gene of BACE protein using ... expression of BACE protein was identified in the neuronal cells of the brain, for preliminary experiments we have chosen human embryonal kidney (HEK293) cells as well as HEK293sw cells overexpressing...
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Báo cáo khoa học: Tumour necrosis factor-a attenuates insulin action on phosphoenolpyruvate carboxykinase gene expression and gluconeogenesis by altering the cellular localization of Foxa2 in HepG2 cells pptx
... prevention of the effect of insulin on Foxa2 localization and leading to the abrogation of insulin inhibition of gluconeogenesis and glucose output in HepG2 cells Although TNFa-mediated inhibition of insulin ... decrease in Foxa2 occupancy of the PEPCK promoter This decrease was significantly (P < 0.01) attenuated in cells preincubated in the presence of TNFa prior to insulin incubation In cells incubated in ... to insulin, insulin signalling is prevented, resulting in abrogation of this inhibitory effect on PEPCK expression PEPCK overexpression, in turn, has been shown to attenuate insulin signalling...
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Báo cáo khoa học: Oxygen control of nif gene expression in Klebsiella pneumoniae depends on NifL reduction at the cytoplasmic membrane by electrons derived from the reduced quinone pool doc
... subsequent selection of single colonies onto plates resulted in loss of the integrated plasmid with an integration frequency of the interrupted nuoCD region in 50% of the integrants fdnG mutant ... containing the anaerobic respiratory chain (Figs and 2) Three other lines of evidence derived from in vivo and in vitro studies of nif regulation further supported our model: first, analysis of ... majority of the flavoprotein NifL in the mutant strains was not reduced at the cytoplasmic membrane resulting in high amounts of cytoplasmic NifL and thus in significant inhibition of NifA in the...
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Báo cáo y học: "Differential gene expression of bone anabolic factors and trabecular bone architectural changes in the proximal femoral shaft of primary hip osteoarthritis patients" pptx
... findings [24] This indicated an increased extent of both bone formation and bone resorption with age, which together suggest an increase in the rate of bone turnover with ageing in control individuals ... Why does bone matrix contain non-collagenous proteins? The possible roles of osteocalcin, osteonectin, osteopontin and bone sialoprotein in bone mineralisation and resorption Cell Biol Int 1994, ... expression in OA and a decrease in controls [16] This finding is supportive of an increase or maintenance of bone volume in OA individuals versus the agedependent bone loss in the general population...
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Báo cáo y học: " Profiles of cytokine and chemokine gene expression in human pulmonary epithelial cells induced by human and avian influenza viruses" doc
... hours postinfection At hours post-infection, the level of previously elevated cytokines/chemokines still remained at several folds of induction In contrast to the prominent induction of cytokines/chemokines ... quantitative differences in the profiles of cytokines/chemokines induced by influenza viruses of different pathogenicity H5N1 was a more potent inducer of inflammatory cytokines/chemokines; particularly ... Cytokine/chemokine protein profiles following infection To verify whether changes at the mRNA level were translated to protein level, the protein concentrations of cytokines/chemokines in cell...
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Báo cáo y học: "Common gene expression strategies revealed by genome-wide analysis in yeast" ppt
... of Analysis transcriptional regulation Analysis of transcriptional regulation Sliding window representation of absolute percentile difference between TR and RS variables (x-axis) against fold-change ... enrichment of genes belonging to protein complexes in the profiles with a predominance of TR over RS (Table 2) It is accepted that proteins belonging to the same complex must be present in similar ... Gerstein M: Analysis of mRNA expression and protein abundance data: an approach for the comparison of the enrichment of features in the cellular population of proteins and transcripts Bioinformatics...
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Báo cáo sinh học: "Localization of leucocyte interferon gene in the q2.5 region of pig chromosomel by in situ hybridization" potx
... number of grains pointed in this region is so high, that we could have reduced the number of spreads scored to one third and still maintain a significant signal In the first experiments, only 5% of ... by in situ hybridization Cytogenet Cell Genet 39, 220-223 Lefevre F & Labonnardi6re C (1986) Molecular cloning and sequencing of a gene incoding biologically active porcine interferon alpha J Interferon ... explain the efficiency of in situ hybridization This observation confirms the one given by Lin et al 1985 We have also modified the washings after hybridization They are less stringent and, in...
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Báo cáo y học: "Differential gene expression in HIV/SIV-associated and spontaneous lymphomas"
... of immunoglobulin gene, γIg – the γ-chain of immunoglobulin gene, IFN-ind – the interferone-inducible gene 6-16, IL4R – the interleukin receptor gene, TAP2 – the transport protein gene, hnRNP ... may be involved in malignant transformation of B cells in HIV/SIV-associated DLBCL We have shown that the gene coding for the small subunit of calcium-dependent cysteine proteinase calpain (capn4) ... leukemia cells and might be associated with malignization of these cells [26] Recent studies of unusual effects of calpains in cancer cells [27-29] suggest that calpain participates in cell malignization...
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Báo cáo y học: "Transporter Molecules influence the Gene Expression in HeLa Cells"
... cannot determine the global players involved in such complex phenotypes Integration of the gene expression profiling with specific modulation of gene expression in relevant signalling pathway ... Analysis Software and are described in the following part according the definitions of the Illumina’s (ILL) data as well as of the Illumina’s annotations (table 2) http://www.medsci.org Int J Med ... order to minimize off-target effects and interpretation of results, perhaps these findings could contribute to a better interpretation of gene expression data, a better choice of suitable individual...
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Ambient particulate air pollution induces oxidative stress and alterations of mitochondria and gene expression in brown and white adipose tissues ppt
... considerable interest in “brown-like adipose cells in WAT These so called “brite” cells are present in WAT as evidenced by the presence of UCP1 expressing cells in WAT Studies in cell culture indicate ... long chain fatty acids (Elovl3), type iodothyronine deiondinase (Dio2), homeobox C9 (Hoxc9), insulin-like growth factor binding protein (Igfbp3), dermatopontin (Dpt), and b-actin are showed in Table ... deviation inflammatory response and IR development, were examined in mice As shown in Figure 3, PM 2.5 exposure induced a marked increase in macrophage (F4/80+ cells) infiltration in eWAT Next,...
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Báo cáo khoa học: Diverging regulation of pyruvate dehydrogenase kinase isoform gene expression in cultured human muscle cells pot
... treatments, cells were incubated in serum-free media containing 0.2% (w ⁄ v) bovine serum albumin (BSA) for a minimum of h Molecular cloning Isolation of RNA from muscle cells was performed using TRI ... not involved in transducing the insulin-to-PDK transcriptional signal The ability of FEBS Journal 272 (2005) 3004–3014 ª 2005 FEBS U0126 to inhibit insulin-stimulated phosphorylation of MAPK in ... the mammalian target of rapamycin (mTOR) GSK3 is inactivated in response to insulin via a PtdIns3K ⁄ protein kinase B (PKB)dependent pathway [27,28] Involvement of GSK3 in FEBS Journal 272 (2005)...
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Báo cáo khoa học: Gene expression in response to endoplasmic reticulum stress in Arabidopsis thaliana ppt
... phosphorylating the specific serine residue of eIF2a [51] to protect cells from the stress P58IPK inhibits PKR-mediated translational arrest by inactivating the kinase by binding to the domain of PKR ... ERSE-like Xbp1 binding-like P-UPRE ERSE-like ERSE-like ERSE-like Xbp1 binding-like Xbp1 binding-like ERSE-like – ERSE-like Xbp1 binding-like Xbp1 binding-like ERSE-like – ERSE-like – Xbp1 binding-like ... purification of the recombinant luminal domain of At SEL-1L BL21(DE3) cells were transformed with pET-30/At SEL-1L The expression of the putative luminal domain of At SEL-1L was induced by the addition of...
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Báo cáo khoa học: Wild-type p53 enhances annexin IV gene expression in ovarian clear cell adenocarcinoma docx
... intron of the ANX4 gene in OVTOKO and OVISE cells but not in MCAS cells, indicating that endogenous p53 protein directly binds to the ANX4 gene in CCA cells To verify the involvement of p53 in ... altering activity in MCAS cells, suggesting that this region contains an element involved in CCA-specific expression of ANX4 In this region, the presence of putative transcription factor-binding ... endogenous p53 actually binds to these regions in CCA cells, we performed chromatin immunoprecipitation (ChIP) assays using PCR analysis of the p53 binding domains regulating ANX4 and p21 after...
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Báo cáo khoa học: Differential gene expression in periportal and perivenous mouse hepatocytes potx
... expression in mouse liver A Braeuning et al enzymes of serine ⁄ glycine metabolism: Glycine decarboxylase, a component of the glycine dehydrogenase complex that converts glycine to serine, is predominantly ... surrounding the central veins [4] Previous studies on zonation of metabolic pathways in the liver were mainly focused on analysis of protein expression or measurements of enzyme activities in rat ... hepatocytes in the expression of genes encoding key enzymes of zonated pathways of intermediary metabolism This holds particularly true for genes encoding enzymes playing a role in pathways that...
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Báo cáo khoa học: Effects of juvenile hormone on 20-hydroxyecdysone-inducible EcR, HR3, E75 gene expression in imaginal wing cells of Plodia interpunctella lepidoptera ppt
... ligandbinding domains, but each has its own isoform-specific segment in the N-terminal region of A/B domain which contains a transactivating domain [40] The predicted sequence of A/B domain of PIEcR ... expression of ecdysone receptor B1 isoform in imaginal wing cells of P interpunctella at the last larval instar In the fifth larval instar of M sexta, it has been reported in vitro that 20E induced ... amino acid identities (especially with the B1 isoform) of other insect EcRs and a strong degree of identity was confined to the two ends of N-terminal region of the A/B domain Using a B1 isoform-specific...
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Báo cáo khoa học: Effect of ecdysone receptor gene switch ligands on endogenous gene expression in 293 cells docx
... Panguluri et al logue of D melanogaster DabIP The DabIP participates in a signaling complex containing various proteins involved in brain development as well as other aspects of adult brain function [55] ... Rab proteins constitute a subfamily of Ras-related GTP-binding proteins that are localized in distinct intracellular compartments [24] Mutations in the Rab gene can alter the morphology of entire ... protein gene showed two-fold up -regulation compared to two-fold down -regulation in qRTPCR analysis (Fig 8B,C) The pathways and interactions of this hypothetical protein are not known In 293 cells...
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Báo cáo hóa học: "Prognostic Impact of MiR-155 in Non-Small Cell Lung Cancer Evaluated by in Situ Hybridization" pot
... semiquantitatively for cytoplasmic staining on computer screen The dominant staining intensity in tumor cells was scored as: = negative; = weak; = intermediate; = strong (Figure 1) In case of disagreement (score ... now being exploited in cancer, attempting to modulate their expression, reintroducing microRNAs lost in cancer, or inhibiting oncogenic microRNAs by using anti-micro oligonucleotides [38] In a ... high expression levels in stromal cells but not in tumor cells [36] Using RNA extracts from whole tumors, this finding would easily be missed Strengthening the relevance of our miR-155 expression...
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báo cáo hóa học:" Site-specific analysis of gene expression in early osteoarthritis using the Pond-Nuki model in dogs" pot
... area of the condyle The areas of India ink staining were outlined using a permanent marker Tracings of the India ink-stained tibial and femoral condyles were evaluated without knowledge of dog ... assessments The lamina splendens was not disrupted in any location based on lack of India ink staining, indicat- ing that AC surface integrity was maintained for two weeks in the dogs in this study ... Increases in COX-2 and INOS in regions most affected by joint instability were consistent with signaling events that typically occur in OA joints[50,51] The concurrent and often colocalized increases...
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Báo cáo hóa học: " Altered gene expression in asymptomatic SHIV-infected rhesus macaques (Macacca mulatta)" pdf
... CINP NUFIP1 IQGAP2 nucleosome assembly protein 1-like destrin proline rich utrophin histidine-rich calcium-binding protein anillin GTP-binding protein, member RAS oncogene family alpha tubulin ... down regulation of another molecular binding protein, 15 kDa selenoprotein (SEP15, ID: AA521350) Reduced level of selenoprotein in cells is a known marker of in vitro infection of SHIV [12] Our ... (Telechem Inc., OR) were used for printing the cDNA clones using 12 × pin format, on a Virtek chip writer professional microarrayer in KemTek, Inc, MD The printed slides underwent UV cross-linking,...
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