... the forward primer 5¢-GGAATTCCATATGCAACCCACTCATACGCTAACAAG-3¢(with the NdeI restriction recognition sequence underlined) and the reverse primer 5¢-CGGGATCCTTATTTTAATTTTACCTGATTGAAGGTTGAG-3¢ ... recognitionsequences for cloning into pG58 was: 5 ¢-GCTACGTTTAAAGCGATGATGAGACCAGAACCCCCACATCACG-3¢ (forward primer with DraI site underlined) and 5¢-CGGAATTCTTAGTGACCGAGGCGGCCCCTGCC-3¢(reverse primer ... crystallographic two-fold. The data and refinementstatistics are shown in Table 2. The Ramachandran plothas 96.9% of the residues in the most favored region and 3.1% in the additional allowed...
... behalf of the American Association for the Study of Liver DiseasesORIGINAL ARTICLETuberculosis in Liver Transplant Recipients: A Systematic Review and Meta -Analysis of Individual Patient DataJon-Erik ... the case-fatality rate. For high-risk transplant candidates, isoniazid appears safe and isprobably effective at reducing MTB reactivation. All liver transplant candidates should receive a tuberculin ... Sugawara Y, Nakajima J, Kishi Y, Niiya T,Kaneko J, Makuuchi M. Resection ofa pulmonary lesionafter liver transplantation: report ofa case. Surg Today2005;35:976-978.37. Alothman A, Al Abdulkareem...
... process a practical certainty. But actually they do not speak with a certain voice—especially because prima-facie adequate external fac-tors are always with us and however we may treat them ... i.e., adaptation within the fundamental data of the system. Ad-aptation may, however, consist in altering some of those data, and such creative response belongs to the class of internal change. ... Mar-shallian tradition and to make use of time in order to define another type of imperfection of equlibrium. What was meant above was the case ofa system so circumstanced as never to reach...
... Free ligandswere adsorbed on charcoal, and the absorbance spectrawere recorded. Concentration of appearing IF–CNCbl wascalculated by comparison with the standards IF–H2OCbl and IF–CNCbl according ... and fixation of the ligands by IF. Dissociation of IF–CBC was biphasic, and existence of multiple protein–analogue complexes with normal and partiallycorrupted structure may explain this behaviour. ... adenosylcobalamin inpatients diagnosed with various malignancies. MayoClin Proc 75, 568–580.4 Bagnato JD, Eilers AL, Horton RA & Grissom CB(2004) Synthesis and characterization ofa cobalamin-colchicine...
... of documents and vocabulary size are muchlarger than the size of latent semantic class variables.Thus, latent semantic class variables function as bot-tleneck variables to constrain word occurrences ... 39th Annual Conference on Association of Computational Linguistics (ACL), 124-131.E. Charniak, K. Knight and K. Yamada. 2003. Syntax-based language models for statistical machine transla-tion. ... standard MapReduce paradigm (Dean and Ghemawat, 2004): the corpus is first divided and loaded into a number of clients, and n-gram countsare collected at each client, then the n-gram countsmapped and...
... study are shown in Fig. 2.Asp140, Asp142, Glu144 and Asp215 were mutated indi-vidually to asparagine and alanine, Tyr10 and Tyr214 werereplaced by Phe, and Ser93 was replaced by alanine. Allclones ... ThepK a of Asp140 is much lower than that of Asp142 and Glu144 in all situations where all three residues are present and the one proton shared by Asp140 and Asp142 appearsto remain on Asp142 ... glycosyl-transferase elucidate catalysis in the alpha-amylase family. Nat.Struct. Biol. 6, 432–436.16. Terwisscha van Scheltinga, A. C., Armand, S., Kalk, K.H., Isogai, A. , Henrissat, B. & Dijkstra,...
... engaged in a strategy that places the company as major player in the regional mobile market place. With recent expansion into Nigeria, Rwanda, Swaziland, Cameroon and Uganda, the MTN brand ... (FMO of the Netherlands, Development Bank of Southern Africa and DEG of Germany) and US$120-million of senior debt from commercial banks (Barclays Bank plc and the Standard Bank Group). EAIF has ... technical and financial assistance directed towards national and regional capacity building, technology transfer on mutually agreed terms, cooperation in R&D programmes and exchange of know-how.”...
... (Stratagene, La Jolla, CA,USA). The primers used were: 5¢-TATATCATTCAGGATTATTTGTATCTTTTAGAATACGCTAAGGTG-3¢ (forward, the mutagenesis codon underlined) and 5¢-TTAGCGTATTCTAAAAGATACAAATAATCCTGAATGATATAAAAAC-3¢ ... enzyme assay for transglutaminaseactivity. Anal Biochem 274, 141–144.25 Gasteiger E, Hoogland C, Gattiker A, Duvaud S,Wilkins MR, Appel RD & Bairoch A (2005) ProteinIdentification andAnalysis ... of degradative enzymes,such as the alkaline protease aprE, at the tran-scriptional level [6] and, for that reason, TenA is oftenclassified as ‘putative transcriptional regulator’ (http://au.expasy.org/).A...
... 5¢-CCTGATCGACGCACGAGT-3¢ and GT1-R,5¢-TTTTGCAAGTCATAGTAATCAGTTT-3¢ for GT-cDNA1 (2150 bp); and GT2-F, 5¢-CACCAGCAACTACCTGATCGA-3¢ and GT2-R, 5¢-CACAAAATATGCTTCCAAGTGC-3¢ for GT-cDNA2 (3043 bp).Construction and ... A, 5¢-TGTCAGTCCTGTACAAAGAC-3¢ and Primer B, 5¢-CATCAGGCTTCCCCATA-3¢. Gene-specific nested primersfor 3¢-RACE were: Primer C, 5¢-CCAGTGTCCCCATCATCAG-3¢ and Primer D, 5¢-GGCAATTTTAAAGTCATTATGGCGCAAA-3¢. ... introns, and anopen reading frame (ORF) of 1599 bp encoding a poly-peptide of 533 amino acids, with a predicted molecular mass of 57 kDa anda pI of 8.34.BLASTX analysis showed thatthe ORF shared...
... evidence against a verb-raising analysis for German. Processing considera- tions also speak against this approach: we would have to postulate that German speakers can either use the German center-embedding ... this approach to the extraposition cases, I will recast it in terms ofa closely related automaton, the Bottom-up EPDA (BEPDA) ~. The BEPDA consists ofa finite-state control andofa stack of ... of stacks. There are two types of moves: either an input is read and pushed onto a new stack on top of the stack of stacks, or a fixed num- ber of stacks below and above a designated stack...
... CCT were quantitated by the use ofa phosphori-mager and expressed as a fraction of the maximum amount of boundlabeled tubulin and plotted as a function of the ratio of labeled : un-labeled tubulin.Ó ... probably accumulated in order to allow microtubulespolymerization and stability at the low temperature of theAntarctic sea water ([6], S. Pucciarelli and C. Miceli,unpublished results). An analysis ... determined by Incla`n and Nogales [47]. Arrows and rectangles indicate b-sheets and a- helices, respectively. The labels ÔLÕ and ÔMÕindicate residues involved in longitudinal and lateral contacts between...