4 1863le structural and enzymatic proteins and the ltr
Báo cáo y học: " Human endogenous retrovirus HERV-K(HML-2) encodes a stable signal peptide with biological properties distinct from Rec" pps
... Retrovirology 2009, 6:17 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 ruses with complex mRNA expression and particle formation Proc Natl Acad Sci USA 1993, 90 :44 80 -44 84 Ono M, Yasunaga ... anchor the proteins by transferring it across the ER membrane, leaving the C-terminus of the protein in the cytoplasmic side of the ER Conversely, type II SPs retain the N-terminus of the protein ... activity The release was a timeand temperature-dependent process, and SPRem/p 14 was found in the nucleolar fraction for at least 90 minutes [ 54] It is currently not known whether MMTV p 14/ SPRem and...
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Báo cáo khoa hoc:" Comparison of nanoparticle-mediated transfection methods for DNA expression plasmids: efficiency and cytotoxicity" potx
... 1. 04* Plano®-AuNP 72.99 ± 39.32* 64. 65 ± 14. 19* 1 54. 49 ± 28.71 83.81 ± 8. 34* LAG-AuNP S1 126.19 ± 41 .31 1 74. 86 ± 18. 54 103.00 ± 21. 84* 193 .48 ± 14. 05 LAG-AuNP S2 98.95 ± 25.09* 200.93 ± 7.52 140 .53 ... 15.79 MALipofection 132. 24 ± 21.05 153.30 ± 12.38 153.17 ± 47 .41 179.62 ± 24. 20 MATra-A 165.15 ± 42 .89 145 .88 ± 40 .31 143 .72 ± 22.50 153.77 ± 13.07 MTH53A Cells 191. 84 ± 25.75 188.01 ± 20.11 ... nm and d90 = 43 .4 nm, Feret diameter: 14 ± nm) and size (hydrodynamic sizes: d50 = 52 .4 nm and 90 = 78.6 nm, Feret diameter: 41 ± nm) dynamic light scattering using a Malvern Zetasizer ZS and...
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Báo cáo y học: " Proviral integrations and expression of endogenous Avian leucosis virus during long term selection for high and low body weight in two chicken lines" ppt
... 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 Sacco MA, Flannery DM, Howes K, Venugopal K: Avian endogenous retrovirus EAV-HP shares regions of identity with avian leukosis virus subgroup J and ... as the sequence analysis LB and UL did the integration analysis PBS produced the chicken lines and conceived the project together with LA and FH FH and LA supervised the work and FH wrote the ... 305/27 34 97.7 20.0 12.9 10 .4 11.2 210 207/7612 96.2 18 .4 13.6 14. 6 14. 4 757 757/5 842 99.1 8.3 5.7 6.8 6.2 588 40 9/5 842 100 6.9 4. 9 7.8 6.9 368 153/5 842 100 2.0 1.9 2.0 1.9 2567 2120/77 04 92.3...
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Reagents and instrumentation
... between these aspects Processivity is a measure of the affinity of the enzyme for the template strand The stronger the interaction, the more processive the polymerase should be, and so the more ... denatures 42 PCR resulting in a ‘Y-shaped’ DNA where the stalk of the Y is the GC-clamp and the tails are the single strands of the target region This Y-shaped molecule becomes entangled in the gel ... template strand If the nucleotide is not correctly paired then the 3′→5′ activity will remove the 3′-nucleotide and the 5′→3′ synthesis activity will introduce a new nucleotide The presence of the correct...
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Tài liệu Báo cáo " synthesis, cloning and expression in escherichia coli of a gene coding for Mcoti-ii " ppt
... technique and sequencing PCR product of recombinant plasmid DNA was shown on Fig 8, it corresponded to the expected size (#114bp) and showing correct sequence (Fig 9) 540 520 500 48 0 46 0 44 0 42 0 40 0 ... codon and Xho I restriction site was introduced on the end of the proposed synthetic trypsin inhibitor gene The sequences of the forward and reversed primers are shown on fig and of the synthetic ... 44 0 42 0 40 0 380 360 340 320 300 280 260 240 220 200 180 160 140 120 100 80 60 40 20 bp Maker 500 40 0 300 200 1 14 y = -66613x + 2 946 25x - 506731x + 43 1852x - 186 046 x + 33174x 100 80 R = 0.9998...
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Tài liệu Báo cáo khoa học: Restricted localization of proline-rich membrane anchor (PRiMA) of globular form acetylcholinesterase at the neuromuscular junctions – contribution and expression from motor neurons doc
... regard to the AChE molecular form, the AChE G1 and G4 forms were predominant in embryonic muscles (Fig 2D) In mature muscles, the relative proportion of the G1 and G4 forms was reduced and the asymmetric ... bands of approximately 20 and 16 kDa, respectively: these protein bands corresponded to the predicted size of the recombinant proteins (Fig 1A) The PRiMA antibody, however, recognized only the ... cord and of G1, G4 and A12 forms in the tibialis (Fig 8C) Thus, denervation induced a decrease in PRiMA and G4 AChE in the spinal cord and muscle To investigate the contribution of the motor neuron...
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Tài liệu Báo cáo khoa học: Nuclear receptors in the mosquito Aedes aegypti Annotation, hormonal regulation and expression profiling ppt
... AM77 344 2 NT P P P P AF230281 AY 345 989 AF3052 14 AM77 344 3 AF059026 AF059027 AF059028 AF305213 AF3052 14 P P NR NT NR NR S NR S BN001173 BN0011 74 BN001175 BN001178 AM77 344 4 AM77 344 5 AF3032 24 P NR ... testing FEBS Journal 276 (2009) 1233–12 54 ª 2009 The Authors Journal compilation ª 2009 FEBS 1251 Nuclear receptors in Aedes aegypti 40 41 42 43 44 45 46 47 48 49 50 51 52 J Cruz et al tissue-specific ... AaHNF4A ** 40 30 20 10 12 50 * 10 *** 30 *** 20 10 12 AaHNF4B AaHNF4A 40 AaHNF4B 10 8 6 4 2 0 200 200 AaHNF4C 160 120 80 80 40 * 160 120 AaHNF4C 40 0 CM 0h CM 20E Chx 20E + Chx CM 6h 0h CM 20E 4h...
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Tài liệu Báo cáo khoa học: Cloning, characterization and expression analysis of interleukin-10 from the common carp, Cyprinus carpio L. docx
... CAD6 244 6 CAD67773 NP_000563 AAC 647 05 CAA43090 A 348 53 43 .2 37.6 28.0 28.5 25.2 25.2 45 .7 39.3 26.6 26.6 25.1 25.1 CAA 248 63 27 .4 AAB26 148 28.0 CAA 248 63 27.0 26.9 26.6 25.7 AAG16755 NP_0611 94 AAK6 246 8 ... (AAK6 246 8), IL- 24 (AAG4 140 1), IL-26 (NP_060872) and interferon-a (P01579); from torafugu IL-10 (CAD6 244 6); from spotted green pufferfish IL-10 (CAD 67773), IL-20 (AY2 945 57) and IL- 24 (AY2 945 60); and ... to their positions The arrowheads depict the residues important for the structural core of the IL-10 gene The underlined amino acid residues are the signal sequences of the respective genes The...
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Tài liệu Báo cáo Y học: Purification, characterization, cloning, and expression of the chicken liver ecto-ATP-diphosphohydrolase pot
... protein)1 pH 7 .4 Addition ATPase None 0.1% NP -40 10 mM azide 0.1% NP -40 + 10 mM azide 0.26 0. 84 0.19 0.59 pH 6 .4 ± ± ± ± ADPase 0. 04 0.28 0. 04 0.16 ATPase 0.05 0.28 0.01 0.10 0. 24 0.75 0. 14 0 .40 ± ± ... 6 .4 with mM MgCl2, mM ATP or mM (ADP) in the absence and presence of the indicated concentrations of sodium azide.MgATP hydrolysis at pH 7 .4 (s) and 6 .4 (d); MgADP hydrolysis at pH 7 .4 (h) and ... ecto-ATPDase and the deduced primary sequence Nucleotide numbers are on the left side and amino-acid residue numbers are on the right side of the figure The transmembranous domains of the protein at the...
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Report of the Special Rapporteur on the promotion and protection of the right to freedom of opinion and expression, Frank La Rue* ppt
... Telecommunication Union launched the “Connect the World” project .45 Another initiative to spread the availability of ICTs in developing countries is the “One Laptop Per Child” 41 42 43 44 45 “Key Global Telecom ... 4( f) E/CN .4/ 1998 /40 ; E/CN .4/ 1999/ 64; E/CN .4/ 2000/63; E/CN .4/ 2001/ 64; E/CN .4/ 2002/75; E/CN .4/ 2005/ 64; E/CN .4/ 2006/55; A/HRC /4/ 27; A/HRC/7/ 14 See para for further information International Telecommunication ... Megabit per second (broadband level). 54 The Special Rapporteur also takes note that according to a 46 47 48 49 50 51 52 53 54 18 E/CN .4/ 2006/55, 30 December 2005, para 34; “Map,” One Laptop per...
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The CMU Pose, Illumination, and Expression (PIE) Database potx
... filtered There are therefore no cases of the subjects either blinking or grimacing during the capture sequence, unlike in the Yale database (where the flashes are also much closer.) On the other hand, ... the room lights, the other major differences between these parts of the database are: (1) the subjects wear their glasses in Figure (if they have them) and not in Figure 5, and (2) in Figure ... calibration and other processing: Head, Camera, and Flash Locations: Using a theodolite, we measured the xyz-locations of the head, the 13 cameras, and the 21 flashes See Figure for an illustration The...
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Báo cáo khoa học: Cloning and expression of the first nonmammalian interleukin-11 gene in rainbow trout Oncorhynchus mykiss pdf
... CT-1, Mouse, Q60753 40 3 9 84 9 84 9 84 636 9 84 9 84 840 726 805 CNTF, Chicken, Q02011 CNTF, Pig, O02732 CNTF, Rat, P202 94 CNTF, Mouse, P51 642 CNTF, Human, P2 644 1 CNTF, Rabbit, P1 41 88 Fig A phylogenetic ... exogenous hydrogen peroxide and killing by activated rainbow trout, FEBS Journal 272 (2005) 1136–1 147 ª 2005 FEBS Rainbow trout interleukin-11 38 39 40 41 42 43 44 45 46 47 48 Oncorhynchus mykiss ... N-glycosylation sites and cysteine residues present in the two fish molecules, and the three conserved residues (K42, M59 and K99) in the mammalian mature IL-11 protein, are in bold and underlined The conserved...
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Báo cáo Y học: Cloning and expression of sterol D14-reductase from bovine liver potx
... BE7567 34, BE7 545 56 and AW427392) [ 34] The bovine cDNA was synthesized by RT-PCR using synthetic primers based on the EST sequences, cloned into the pCR2.1 vector, and sequenced on both strands The ... sequences Microbiology 145 , 144 3± 145 1 24 Fieser, L.F & Ourisson, G (1953) Cholesterol and companion IV Oxidation of D7 sterol with selenium dioxide J Am Chem Soc 75, 44 04 44 14 25 Mancini, A., Del ... thaliana sterol D7-reductases, respectively, and 44 % to S cerevisiae sterol D 24( 28)-reductase] The EFGGx(2)G signature of sterol D 24( 28)-reductase and D 14- SR and the LLxSGWWGx(2)RH signature of sterol...
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Báo cáo khoa học: Identification and expression of the first nonmammalian amyloid-b precursor-like protein APLP2 in the amphibian Xenopus laevis ppt
... exclusively in the brain and further displays a number of structural features not present in the other members of the APP superfamily, such as the absence of the exon encoding the KPI domain and the lack ... Koch, C., Sisodia, S., 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 Tremml, P., Lipp, H.P., Wolfer, D.P & Muller, U (2000) Mice with combined gene knock-outs reveal essential and partially redundant ... in murine APLP2 [ 24] Furthermore, the transmembrane domains and the cytoplasmic tails of the Xenopus and mammalian proteins are 91% and 100% identical, respectively, including the GYENPTY sequence...
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Báo cáo khoa học: Identification and expression analysis of an IL-18 homologue and its alternatively spliced form in rainbow trout (Oncorhynchus mykiss) doc
... Horse Human Mouse Pig Rat Chicken Fugu Tetraodon Trout 42 .0 41 .4 45.6 42 .1 44 .3 42 .6 41 .5 42 .9 40 .5 42 .4 100 193 193 193 193 192 192 1 94 198 189 189 199 157 157 157 157 157 157 158 160 158 158 ... 41 46 % similarity with the IL-18 proteins from mammals, 42 .9% with chicken, 40 .5% with Fugu, and 42 .4% with tetraodon (Table 2) It has lower similarity with the other members of the IL-1 family; 30–38% ... to the search for similarities in the amino acid sequence of two proteins J Mol Biol 48 , 44 3 45 3 44 Nielsen, H., Engelbrecht, J., Brunak, S & von Heijne, G (1997) Identification of prokaryotic and...
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Báo cáo khoa học: Cloning and expression of a tomato cDNA encoding a methyl jasmonate cleaving esterase pdf
... 94 °C, at 40 °C and at 72 °C, followed by 30 cycles of 30 s at 94 °C, at 45 °C and at 72 °C Gel electrophoresis, DNA elution and modification was carried out according to standard protocols [ 24] ... Mono Q HR 5/5 Talon resin 2 044 712 67.5 1.9 0.052 3352 1317 652.7 100.6 24. 1 1. 64 1.85 9.67 54. 39 46 4.3 1.13 5.89 33.16 283.11 100 27.7 19 .4 3.0 0.8 Fig SDS/PAGE and Western blot analysis of ... 5 24. 93 Da and the pI ¼ 5.52 The calculated mass of the encoded protein corresponds well with the molecular mass of 28 000 determined by SDS/PAGE for the purified plant protein Comparison of the...
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Báo cáo khoa học: "Sentence and Expression Level Annotation of Opinions in User-Generated Discourse" potx
... fact, then the aim is to mark the target and label the polarity of the evaluation If the sentence is opinionated, then, the aim is to mark the opinion expression span, and label its polarity and ... impression about the school, while the other one treated them as opinions Table 4: Inter-annotator agreement at the expression level We observe that the strength of the opinionexpression and the polar ... intensity), and to link it to the target and the holder Figure presents the expression level scheme At this stage, annotators mark text spans, and are allowed to assign one of the five labels to the marked...
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Báo cáo khoa học: Isolation, characterization and expression analysis of a hypoxia-responsive glucose transporter gene from the grass carp, Ctenopharyngodon idellus potx
... (Q07 647 ); rabbit GLUT3 (Q9XSC2); human GLUT3 (AAB61083); dog GLUT3 (P47 842 ); bovine GLUT3 (AAK70222); sheep GLUT3 (P47 843 ); brown trout btGLUT4 (AAG12191); bovine GLUT4 (Q279 94) ; rat GLUT4 (P19357); ... of 57 kDa and a pI of 8. 34 A database search using BLASTX showed that the ORF shared high sequence identity with the GLUT1 (57–59%), GLUT3 (59–60%) and GLUT4 (55–59%) proteins, and moderate ... AY23 147 6) spans 11 kb of genomic DNA and contains 12 exons and 11 introns The 5¢-UTR (203 bp) is contained in exons and 2; the coding region (1599 bp) is distributed across exons 2–12 and the...
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Báo cáo Y học: Cloning and expression of two novel aldo-keto reductases from Digitalis purpurea leaves potx
... AAC23 646 and CAB88350, respectively) and 67% with the alfalfa aldose/aldehyde reductase (accession number X97606) Furthermore, we found a 45 47 % conservation in amino-acid residues with AKR4 proteins ... DpARs these amino acids are G42, D47, E55, G59, K81, P116, G156, P176, Q180 and S257 Besides these residues, Y52, H1 14 and K256 are almost strictly conserved in the different AKR members Some of these ... (X97606) AR proteins from mammals were used as the outgroup: human (P 145 50), cow (P16116), rat (P07 94) , mouse (P45376) The tree was constructed using the neighbour-joining method [37] and the program...
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