... 2005 The Authors Journal compilation ª 2005 FEBS C Putonti et al 10 11 12 13 14 15 16 17 improve the rate of dengue diagnosis compared to twostep RT-PCR approaches J Clin Virol 30, 297 3 01 Wang ... sequences then would be expected In AF289029, 30ofthe 34 16 -mers that are two changes away fromthe nearest human sequence are unique, and 16 014 of its 21 248 22-mers one change away fromthe nearest ... 17 18 19 20 21 22 20 14 0 0 12 0 0 0 0 0 0 0 0 We also calculated the number of unique sequences for each dengue type (DENV -1) or DENV-2 serotype, as these types comprise the great majority of...
... after the end ofthe breastfeeding period .4 Community mobilization Antiretroviral therapy for infants Early infant diagnosis 18 Option A* Co-trimoxazole for infants 2 011 2 012 2 013 2 014 2 015 Countdown ... HIV, as of May 2 011 Believe it Do it no data 11 The programme framework The implementation framework for theeliminationof new HIV infections among children and keeping their mothers alive will ... prevention of mother-to-child be expressed in terms ofthe number of Countries will conduct a mapping ofthe transmission of HIV programmes andthe new HIV infections in children andthe resources...
... isbn -13 isbn -10 978-0- 511 -18 634-9 eBook (EBL) 0- 511 -18 634-7 eBook (EBL) isbn -13 isbn -10 978-0-5 21- 82087-5 hardback 0-5 21- 82087 -1 hardback Cambridge University Press has no responsibility for the persistence ... Figure 1.1 (cont.) 10 11 12 13 Neanderthals and Modern Humans become isolated from others I have introduced scale here and it is an issue that is central to understanding ecology (Levin, 19 92) and ... understand human evolution, the spread of humankind across the world andthe extinction of archaic populations we must start off from a theoretical evolutionary ecology base and incorporate the...
... concanavalin A [18 ] The 5¢ and 3¢ termini ofthe clone were obtained by anchored PCR This enabled us to clone additional sequences of 14 5 and 518 bp at the 5¢ and 3¢ ends, respectively Thus, the fully ... accession number AB 110 780 Genomic structure of carp IL -10 Using the carp primers IL -10 -Fw2 and IL -10 -Rv2, we amplified a product of1. 4 kbp from carp genomic DNA A 14 03-bp IL -10 genomic sequence ... CAA 4309 0 A34853 43.2 37.6 28.0 28.5 25.2 25.2 45.7 39.3 26.6 26.6 25 .1 25 .1 CAA24863 27.4 AAB2 614 8 28.0 CAA24863 27.0 26.9 26.6 25.7 AAG16755 NP_0 611 94 AAK62468 AAG 414 01 NP_060872 16 .0 19 .0 13 .8...
... incorrect 10 1 (93%) ( 7%) 71 ( 81% ) 17 (19 %) 11 3 (88%) 15 (12 %) 10 6 ( 91% ) 10 ( 9%) 12 8 (76%) 41 (24%) 519 (85%) 91( 15%) √ check mark ‘ ’ indidates that the term passed the test Twenty terms out ofthe ... the filtering step A: the target term existed in the compiled corpus, but was not extracted by automatic term extraction total 10 9 88 12 8 11 6 16 9 610 S 10 18 43 F 0 Evaluation II A C R 14 11 19 ... topics, and persons in Japanese history From these fifty terms, the system collected 610 terms in total; the average number of output terms per input is 12 .2 terms We checked whether each ofthe 610 ...
... task, but with the provision of c o n t e x t s w h i c h are f e l i c i t o u s w i t h one or other ofthe two versions of their examples the with the to NP) In the case ofthe n o n - m i ... between them is the change from "two safes" to "a safe and a j e w e l r y box" This is a c h a n g e which, in theory, a f f e c t s only the cardinality ofthe set of "safes" It was 12 4 found ... a u s e ofthe i n c r e a s e d n u m b e r of t h e s e v i o l a t i o n s It w o u l d appear, then, that much ofthe evidence cited in the literature concerning the resolution of local...
... temperature increases, the native peaks disappear, and concomitantly one can observe the appearance of two peaks at 16 18and 16 83 cm )1, which are typical ofthe formation of an intermolecular antiparallel ... as evidenced by the appearance ofthe spectral bands at 16 83 and 16 18 cm )1 upon decompression (Fig 5C) [25] The changes in absorbance at 16 54 cm )1 have been plotted as a function of pressure at ... b-sheet aggregate [ 21] The thermal stability of b-glucosidase was assessed by plotting the temperature dependence ofthe peak intensity at 16 18 cm )1 (Fig 4B) The melting point ofthe enzyme was estimated...
... lipid-A and two phosphate moieties The ions with m ⁄ z of 15 19.6 and 15 41. 6 are mono- and di-sodiated forms ofthe de-O-acylated LOS andthe ion with m ⁄ z of 14 79.6 is the anhydrous form ofthe ... data of de-O-acylated LOS for the mutant O35Elgt3 showed the presence of ions with m ⁄ z of 14 79.6, 14 97.6, 15 19.6 and 15 41. 6, respectively The ion at m ⁄ z 14 97.6 (M-H)– corresponds to the deprotonated ... moieties The deO-acylated lipid-A unit consists of two GlcNAc and two 3-OH C12:0 groups Among the other ions m ⁄ z of 2654.8, 2694.8 and 2 717 .4 indicates the presence ofthe anhydro form and mono- and...
... Sephacryl S -10 0 Total activity (IU) Protein (mg) Specific activity (IUÆmg )1) Yield (%) Purification fold 2000 50 20 60 50 30 32.6 30. 6 25 11 .9 8.6 25 20 0.7 0.5 1. 3 1. 4 3.6 11 .9 13 17 .2 10 0 94 77 37 30 ... fromthe sequence ofthe extreme 5¢ end of lac1 The full-length cDNA of lac1 was cloned and sequenced as above RT-PCR for semiquantification ofthe lac1 expression levels Total RNA extracted from ... full-length cDNA of lac1 The cDNA contained a predicted ORF of 15 57 bp encoding 519 amino acids (Fig 1) The amino acid sequence from Ala25 to Asp 41 corresponded to the N-terminal sequence ofthe purified...
... 0.85 ± 0 .30 8.2 ± 1. 0 16 ± Av-IDH(28) 6.8 8.3 13 0 0. 013 9 0.39 – 1 930 T Kanao et al (Eur J Biochem 269) Fig Inhibition of Cl-IDH with oxaloacetate (A) Effect of oxaloacetate concentration on the carboxylation ... not shown) and native-PAGE (Fig 1) analyses, andthe specific activity ofthe purified IDH reached 36.0 UÆmg )1 (Table 1) The molecular mass ofthe native enzyme was determined to be 81 kDa by gelfiltration ... ATP-citrate lyase [24] The results demonstrated the heteromeric structure of this enzyme and its role in regulating the direction and flux ofthe RTCA cycle For further understanding ofthe RTCA cycle,...
... (19 94) Role of abnormally phosphorylated tau in the breakdown of FEBS Journal 274 (2007) 5 012 –5020 ª 2007 The Authors Journal compilation ª 2007 FEBS J.-T Du et al 13 14 15 16 17 18 19 20 21 ... pKa1 and pKa2 The pKa value is determined from a fit ofthe phosphorus chemical shifts ofthe without ionic, monoionic and diionic forms ofthe phosphate group as a function of pH [33,34] The ... affects the electrostatic potential and quite often the conformation ofthe modified protein Even in the absence of rearrangement, the change in the electric field and steric hindrance from a phosphate...