... sequencing and determination of theanomeric configurations of the corresponding glycosidiclinkages were performed by degradation with exoglyco-sidases (Fig. 6 and Table 4). All glycans containinggalactosyl ... constituents.Analysis of total KLH glycans In order to take an overview of the complexity of KLHglycosylation, intact glycoprotein was subjected to automa-ted hydrazinolysis. Resulting glycans were pyridylaminatedand ... in the liberation of both N- and O-linkedglycans. In parallel, a total glycan fraction was preparedfrom intact KLH by hydrazinolysis using similar conditions.Pyridylamination of oligosaccharidesChemically...