... presence of competitive inhibitors of O-glycosylation, such as benzyl 2-acetamido-2-deoxy-α-D-galactopyranoside, without adversely affecting cells (9). With the eluci-dation of more sequence data for ... on the blot (e.g., 3,3'-diaminobenzidine, 4-chloronapthol mix forHRP [14]; 5-bromo-4-chloro-3'-indolyphosphate toluidine salt, nitro blue tetrazolium chlo-ride mix for AP). Alternatively, ... nonionicdetergents: 0.05% (v/v) Nonidet P-40 or Tween-20.4. Enzyme substrates: 2,2'-azino-bis(3-ethylbenzathiazaline 6-sulfonic acid) (ABTS) (1 mg/mL,A405nm), O-phenyldiamine (OPD) (1 mg/mL, A492nm),...