... embryos, in which maternal dorsal determinants have been inactivated or removed, have been an effective tool for analyzing From: Methods in Molecular Biology, Vol 127: Molecular Methods in Developmental ... From: Methods in Molecular Biology, Vol 127: Molecular Methods in Developmental Biology: Xenopus and Zebrafish Edited by: M Guille © Humana Press Inc., Totowa, NJ 29 30 Newman and Krieg Fig Developmental ... Green tein factors are to be used in the medium, bovine serum albumin (BSA, Sigma) should be added to 0.1% w/v to block nonspecific protein binding The agarose lining of dissection and incubation...