21 frizell lv ludmila ib isolation purification and cultivation of murine and human keratinocytes methods in molecular biology vol 290 basic cell culture protocols third edition 187 194
KHÓA LUẬN tốt NGHIỆP CHUYÊN NGÀNH CÔNG NGHỆ SINH học KHẢO sát sự ẢNH HƯỞNG của DỊCH CHIẾT từ lá cây SỐNG đời (KALANCHOE PINNATA) lên QUÁ TRÌNH TĂNG SINH tế BÀO CÓ NGUỒN gốc từ DA
... Keratinocyte xây dựng khung tế bào vững chắc nhờ thay đổi biểu của loại vi sợi keratin từ keratin 14 thành keratin 10 Keratinocyte có khả sản xuất protein có vỏ keratin involucrin loricrin ... Gallatin, 1991 + Mối tương tác tế bào giá thể môi trường nuôi cấy được thể qua đoạn dính gắn (integrins) của tế bào, thụ thể của tế bào gắn bám với phân tử chất nền là: fibronectin, laminin, ... (Kalanchoe pinnata) lên trình tăng sinh tế bào nguyên bào sợi da chuột nhắt trắng (Mus muscullus var.albino)” Mục tiêu nghiên cứu Khảo sát khả tách của tế bào hai phương pháp trypsin ấm trypsin lạnh...
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cell cycle control mechanisms and protocols methods in molecular biology - tim humphrey, gavin brooks
... number of posttranslational mechanisms including phosphorylation, inhibition by cyclin-kinase inhibitors, destruction of cyclins, and destruction of the inhibitors at the appropriate time in the cell ... be involved in integrating cell cycle signals and transducing these to regulate the cytokinetic vesicle fusion machinery No homologs of polo-like kinases or aurora kinases, which are all involved ... ubiquitylation of a number of cell cycle proteins including G1 cyclins (Cln1 and Cln2), Cdk inhibitors The Budding and Fission Yeasts 11 (Sic1, Far1, and Rum1), and replication proteins (Cdc6 and Cdc18;...
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methods in molecular biology, v.474. nanostructure design. methods and protocols, 2008, p.268
... Methodology for Creating and Studying Chimeric Proteins Between Fibritin and Triple-Stranded Segments of Fibrous Proteins The methodology for creating and studying chimeric proteins was first developed ... understanding of fibrous proteins In phage T4 fibritin, a 27-amino acid (aa) domain (amino acids 457483 of fibritin) forms a trimeric globular, -propellor-like structure located at the C-terminal ... design and fiber engineering An important source of inspiration for the creation of such proteins are natural fibrous proteins such as collagen, elastin, insect silks, and fibers from phages and viruses...
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Isolation, purification and detection of soyasaponins and their associated bioactivities in cultured hepatocarcinoma cells
... protein kinases B (PKB) plays an important role in mammalian cellular signaling Ara: α-L-arabinopyranosyl B16F10: Murine melanoma cancer cell line BrdU: Bromodeoxyuridine BSA: Bovine serum albumin ... through the induction of apoptosis In Hela cells after four days of treatment, total soyasaponins (0.4 mg/mL) showed an increase in sub-G1 cells (apoptotic cellular fragments) of 10% during cell cycle ... soyasaponins reduced cell growth after 24 and 48 h of treatment Furthermore, treatment increased the percentage of cells in the S phase of the cell cycle while reducing cyclin-dependant kinase-2...
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Báo cáo khoa học: Dual expression of mouse and rat VRL-1 in the dorsal root ganglion derived cell line F-11 and biochemical analysis of VRL-1 after heterologous expression pptx
... brain, F-11 and N18TG2 total RNA Total RNA of rat brain, F-11 and N18TG2 cells was prepared using TRIzol Reagent (Invitrogen) according to the instruction of the manufacturer lg total RNA was incubated ... differentiated DRG cells are present in F-11 cells, e.g release of substance P, the presence of l- and d-opioid receptors, receptors for prostaglandin and bradykinin and L-type calcium channels Recently, ... into the pCRII-TOPO vector (Invitrogen) and sequenced The accuracy of sequences from the open reading frame was checked by DNA sequencing of both strands The resulting sequences of specific bands...
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data analysis in molecular biology and evolution - xuhua xia
... ending at 517, the second coding segment starting from 646 and ending at 1735, and the third coding segment starting from 1933 and ending at 216 5 The complete coding sequence is made by joining ... that the genes in the two sequences each contain three exons, and the final coding mRNA results from the splicing out of the introns and the joining of these three exons The CDS entry in the FEATURES ... protein-coding nucleotide sequences in one format into amino acid sequences in another format A PLETHORA OF COMPUTER PROGRAMS Scientists in the field of molecular biology and evolution use a variety of...
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![progress in nucleic acid research and molecular biology [vol 67] - k. moldave (ap, 2001) ww](https://media.store123doc.com/images/document/14/y/bw/small_h4P4i1s5po.jpg)
progress in nucleic acid research and molecular biology [vol 67] - k. moldave (ap, 2001) ww
... of the sequence of iPLA2fl with the ATP-bindingdomain of some of serine/threonine protein kinases and of tyrosine protein kinases The sequence of iPLA2fl and the consensusof ATP-bindingdolnain ... the ATP-binding domain of protein kinases revealed that the sequence from amino acid residue 431 to 457 of iPLA2fl exhibits similarity to the region of protein kinases involved in ATP binding (Fig ... cahnodulin-binding domains from sequence analyses The amino acid sequences of a number of cahnodulin-binding proteins have been determined, and in many cases the loeations of the binding domains...
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Báo cáo hóa học: "GEOMETRIC AND HOMOTOPY THEORETIC METHODS IN NIELSEN COINCIDENCE THEORY" pdf
... preprint, 2002 [9] U Koschorke, Vector Fields and Other Vector Bundle Morphisms—a Singularity Approach, Lecture Notes in Mathematics, vol 847, Springer, Berlin, 1981 , Linking and coincidence invariants, ... that b and b, when restricted to ∂B, coincide with the corresponding data gi and g i at Ci , i = 0,1 (via vector fields pointing into B along C0 and out of B along C1 ) The resulting set of bordism ... manifolds M1 and M2 have disjoint images) Just as in the case of two disjoint closed curves in R3 the degree of linking can be measured to some extend by the geometry of the overcrossing locus:...
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báo cáo khoa học: " Hypercalcemia and huge splenomegaly presenting in an elderly patient with B-cell non-Hodgkin’s lymphoma: a case report" potx
... writing the manuscript and the collection of data KK undertook some laboratory analysis and endocrine interpretation All authors read and approved the final manuscript Page of Competing interests ... Figure H&E staining (hematoxylin and eosin staining) Ghazi et al Journal of Medical Case Reports 2010, 4:330 http://www.jmedicalcasereports.com/content/4/1/330 Page of Figure A CT scan of the patient ... (doxorubicin) for a total dose of 70 mg, 1.4 mg/m of Oncovin (vincristine) for a total dose of mg per injection and 75 mg of prednisolone daily for five days After the second course of chemotherapy, her...
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Báo cáo y học: "Text-mining and information-retrieval services for molecular biology" pdf
... yeast and on the p53 tumor suppressor and the AP1 transcription factor Some attempts have been made to merge text-mining methods and bioinformatic methods involving sequence analysis into a single ... realize that existing text-mining technologies in biology are focused on identification and linking of functional information of proteins in free text, they are currently not providing automatically ... availability of full-text articles most of the existing text-mining systems for biology are centered on the analysis of abstracts, but changes in publishing policy and increasing access to repositories of...
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COLLEGE STUDENTS PERCEPTIONS OF QUARANTINE AND SOCIAL DISTANCING METHODS IN THE EVENT OF AN INFLUENZA PANDEMIC
... a) isolation of patients and quarantine of contacts, b) social distancing methods, such as closing schools and businesses and canceling public events, and c) increasing personal protection and ... PERCEPTIONS OF QUARANTINE AND SOCIAL DISTANCING METHODS IN THE EVENT OF AN INFLUENZA PANDEMIC In the event of another pandemic influenza, it will be important to understand the public’s perception of ... quarantine, isolation, and social distancing methods will have to be implemented at the beginning of an influenza pandemic (U.S Department of Health and Human Services, 2006c; WHO Writing Group,...
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Excluded volume effects in molecular biology and extracellular matrix biochemistry biophysical considerations and molecular modeling
... drive protein folding In vitro biology: Illustrating the shortcomings of current in vitro cell culture Reverse proteolysis Crowding in the ECM Dependence of the folding rates as a function of concentration ... associations involved in signal transmission, frequently increasing the binding strength by at least an order of magnitude (Wilf & Minton, 1981) 6.4 Macromolecular Crowding on Protein-folding and Stability ... Review of Literature The role of Macromolecular Crowding (MMC) in shaping the cell and extracellular environments and its influence on most biological processes both at the intra- and extra-cellular...
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siRNA, miRNA and their possible roles in molecular therapy of chronic human hepatitis b virus infection and hepatocellular carcinoma
... thereby resulting in the inhibition of the expression of these mRNAs Binding of ASO to its target mRNA generally leads to the blockage of protein translation In some cases, intracellular RNase ... domain initiates the incorporation of a small RNA into RISC by binding the single-stranded 2nt 3’overhang of the siRNA duplexes and the PIWI domain determines the endonuclease cleavage activity of ... intracellular viral proteins were specifically inhibited In the same year, Ying and colleagues published their studies on the effect of transfection of the HBV-inducible cell lines HepAD38 and...
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Application of knowledge discovery and data mining methods in livestock genomics for hypothesis generation and identification of biomarker candidates influencing meat quality traits in pigs
... substrates and enzmes involved in androstenone synthesis is given in Figure 2.6 3α-androstenol and 3β-androstenol are the final metabolites of androstenone in both testis and liver In liver, androstenone ... present, breeding practices in involves a combination of conventional breeding methods and advanced genetic technologies to refine and understand the genetics of favorable characters in livestock ... used in the field of data compression, but index built using BWT allows indexing and searching of large texts in a memory efficient manner FM index is an indexing algorithm utilizing BWT and allows...
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Purification and cloning of PCR products
... preventing DNA damage of the long molecules and enhancing isolation of full-length clones The range of cloning kits and vectors adapted for direct cloning and expression of PCR products is increasing ... PCR Of course one potential disadvantage of the increased number of PCR cycles is the increased possibility of accumulating PCR-mediated mutations in the final PCR products Use of ‘proofreading’ ... visual indicator of the presence and position of an ethanol pellet The Pellet Paint™ NF reagent is compatible with preparation of samples for fluorescent sequencing applications involving dyes...
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Tài liệu Báo cáo khoa học: Purification and sequence identification of anserinase ppt
... contain another peptidase called carnosinase Carnosinase resembles anserinase in hydrolytic ability against carnosine, anserine and homocarnosine, which are unusual dipeptides containing non-a-amino ... trout anserinase Molecular cloning of anserinase and CNDP-like protein Database search Initially we searched for a candidate for anserinase in the GenBank database by blastp using the N-terminal sequence ... distribution of the mRNAs for anserinase and CNDP-like protein in Nile tilapia The possibility of genomic contamination was eliminated by the observation of amplifications spanning the exon–intron...
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Chapter 21 Network Layer: Address Mapping, Error Reporting, and Multicasting pdf
... process the packet immediately 21. 29 Example 21. 5 (continued) 21. 30 21- 3 IGMP The IP protocol can be involved in two types of communication: unicasting and multicasting The Internet Group Management ... messages 21. 18 Figure 21. 11 Redirection concept 21. 19 Figure 21. 12 Query messages 21. 20 Figure 21. 13 Encapsulation of ICMP query messages 21. 21 Example 21. 2 Figure 21. 14 shows an example of checksum ... defines the number of bytes as 64 This is the total number of bytes in the ICMP packet (56 + 8) 21. 24 Example 21. 3 (continued) 21. 25 Figure 21. 15 The traceroute program operation 21. 26 Example 21. 4...
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Báo cáo khoa học: Isolation, characterization and expression analysis of a hypoxia-responsive glucose transporter gene from the grass carp, Ctenopharyngodon idellus potx
... GRR motifs in intracellular loops and 8; glutamate and arginine residues in intracellular loops and 10; the QL motif in transmembrane helix 5; the QLS motif in transmembrane helix 7; and the three ... positions 298 and 299 in loop (Fig 2) that are involved in conformational change of the GLUT protein during transport [25] To date, cDNAs of five GLUT isotypes have been described in fish and sequence ... (1998) Serine-s94 and threonine-295 in the 26 27 28 29 30 exofacial loop domain between helixes and of glucose transporters (GLUT) are involved in the conformational alterations during the transport...
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Water Pollution: Impact of Pollutants and New Promising Techniques in Purification Process doc
... uranium mining and enrichment plants (Rao 2001) The mining and construction industries are major contributors of sediment and acid drainage There are basically four main types of mining impacts ... manufacturing (ii) power-generating (iii) mining and construction, and (iv) food processing industries (Mc Kinney and Schoch 2003) Manufacturing industries like chemical, oil refining, steel etc contribute ... problem in areas where agriculture is becoming increasingly intensified These fertilizers increase the concentration of nitrates in groundwater, leading to high nitrate levels in underground drinking...
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