... classified 14 of the 18 samples (78% accuracy) Secondary validation of the candidate biomarker signature The performance of the candidate biomarkers identified in the training set was further evaluated ... Chantratita N, Wuthiekanun V, Limmathurotsakul D, Thanwisai A, Chantratita W, Day NP, Peacock SJ: Prospective clinical evaluation of the accuracy of 16S rRNA real-time PCR assay for the diagnosis ... 5'-gggaatgagaaagtcctgtcc-3', and reverse primer, 5'-tcaatcttggggatcaggtg-3'; HLA-DRA [GenBank:NM_019111.3] forward primer, 5'-caagggattgcgcaaaag3', and reverse primer, 5'aagcagaagtttcttcagtgatctt-3';...