Transcriptome analysis using RNA seq on response of respiratory cells infected with porcine reproductive and respiratory syndrome virus (PRRSV)

... analysis using RNA- Seq on response of respiratory cells infected with porcine reproductive and respiratory syndrome virus (PRRSV) The porcine reproductive and respiratory syndrome (PRRS) is one ... by RNA- Seq (A) and by real-time PCR (B), gene expression profile of CD86 in infected and non -infected PAMs, detected by realtime PCR (C) and gene expression profile of CD86 in infected and noninfected ... by RNA- Seq (A) and by real-time PCR (B), gene expression profile of CCL4 in infected and non -infected PAMs, detected by real-time PCR (C) and gene expression profile of CCL4 in infected and non-infected...

Báo cáo y học: " Molecular characterization of porcine circovirus 2 isolated from diseased pigs co-infected with porcine reproductive and respiratory syndrome virus" pot

... as: Yi and Liu: Molecular characterization of porcine circovirus isolated from diseased pigs co-infected with porcine reproductive and respiratory syndrome virus Virology Journal 20 10 7 :28 6 ... inoculation of conventional pigs with porcine reproductive and respiratory syndrome virus and porcine circovirus J Virol 20 02, 76: 323 2- 323 9 13 Tian K, Yu Y, Zhao T, et al: Emergence of fatal PRRSV variants: ... 13I 91 22 T 13S 92 134 23 V 9N 12I 36T 151 28 T 17P 169 10R 33S 2G 190 19T 14S 12A 191 5R 13A 27 G 20 6 30I 15K 21 0 13E 14D 21 5 8I 37V 29 34T 11A 40 12 R 10S 88 41Q 4E 100 25 F 20 L 101 9Y 9N 1 02 ORF3...

Báo cáo khoa học: "Comparative analysis of complete nucleotide sequence of porcine reproductive and respiratory syndrome virus (PRRSV) isolates in Thailand (US and EU genotypes)" pot

... losses in the swine industry worldwide including Thailand Porcine reproductive and respiratory syndrome (PRRS) was first evident in the North American countries in 1987 and later in the European ... Figure Sequence alignment of NSP2 of PRRSV viruses (US strains) Sequence alignment of NSP2 of PRRSV viruses (US strains) Deduced amino acids of NSP2 gene of 01NP1 were compared to those of US strains ... European-type porcine reproductive and respiratory virus strains in Spain J Gen Virol 2003, 84:529-534 Stadejek T, Oleksiewicz MB, Potapchuk D, Podgorska K: Porcine reproductive and respiratory syndrome virus...

Báo cáo sinh học: " Role of CD151, A tetraspanin, in porcine reproductive and respiratory syndrome virus infection" pdf

... Figure RNA-binding activity of CD151 in vitro and in vivo RNA-binding activity of CD151 in vitro and in vivo (A) In vito RNA-binding activity of CD151 was demonstrated by Immunoprecipitation/North-Western ... Vanderheijden N, Nauwynck HJ, Pensaert MB: Involvement of the matrix protein in attachment of porcine reproductive and respiratory syndrome virus to a heparinlike receptor on porcine alveolar ... porcine reproductive and respiratory syndrome virus into porcine alveolar macrophages J Virol 2003, 77:8207-8215 Kim JK, Fahad AM, Shanmukhappa K, Kapil S: Defining the cellular target(s) of porcine...

báo cáo khoa học: "Co-existence of acute myeloid leukemia with multilineage dysplasia and Epstein-Barr virus-associated T-cell lymphoproliferative disorder in a patient with rheumatoid arthritis: a case report" docx

... factor, anti-RNP antibody, and anti-SS -A antibody Bilateral hand X-rays showed mild swelling and destruction of the metacarpo-phalangeal (MP) and proximal-inter-phalangeal (PIP) joints He was diagnosed ... Hoshida Y, Xu JX, Fujita S, Nakamichi I, Ikeda J, Tomita Y, Nakatsuka S, Tamaru J, Iizuka A, Takeuchi T, Aozasa K: Lymphoproliferative disorders in rheumatoid arthritis: clinicopathological analysis ... Hematoxylin and eosin (HE) staining and immunohistochemistry analyses of bone marrow (BM), spleen, and lymph nodes Hematoxylin and eosin (HE) staining and immunohistochemistry analyses of bone marrow...

Tài liệu Báo cáo khóa học: Quantitative analysis, using MALDI-TOF mass spectrometry, of the N-terminal hydrolysis and cyclization reactions of the activation 2 process of onconase pdf

... (Met1)-ONC (M23L) by the addition of AAP, as described in the Materials and methods Completion of the hydrolysis was confirmed by the disappearance of the (Met1)-ONC (M23L) signal in the MALDI-TOF mass ... other methods for the production and purification of the enzyme [13] The molecular mass of each purified product, as measured by MALDI-TOF MS, was 11 947.87 2 for (Met1)-ONC (M23L) and 11 838 .21 2 ... by MALDI-TOF MS The addition of the protease is considered the zero time point The relative intensities of the peaks from the mass spectra were used to calculate the fraction of Pyr-ONC (M23L),...

báo cáo hóa học:" Gene and microRNA analysis of neutrophils from patients with polycythemia vera and essential thrombocytosis: down-regulation of micro RNA-1 and -133a" pot

... [32] Figure Gene expression analysis of MPD neutrophils Gene expression analysis of MPD neutrophils Gene expression of neutrophils from MPD patients, healthy subject neutrophils and healthy subjects ... the molecular basis of MPDs, we compared gene and miRNA expression profiles of neutrophils from MPD patients with those from healthy subjects We identified several genes and microRNA whose expression ... http://www.targetscan.org/ and miRBase Targets http:/ /microrna. sanger.ac.uk Gene and MicroRNA Expression Quantitative PCR To validate the microarray analysis, genes and microRNAs were selected for Quantitative PCR Gene...

Báo cáo y học: "Gene ontology analysis for RNA-seq: accounting for selection bias" pdf

... category testing on GO analysis The techniques developed here apply more generally, however, to any analysis that looks for over-representation of some category of interest amongst differentially ... 2005:397-420 Young et al Genome Biology 2010, 11:R14 http://genomebiology.com/2010/11/2/R14 doi: 10.1186/gb-2010-11-2-r14 Cite this article as: Young et al., Gene ontology analysis for RNA-seq: accounting ... precisely estimated for microarray probes at higher intensity levels, so selection bias is likely to exist as a function of intensity Furthermore, most microarray platforms have multiple probes for...

Tài liệu Báo cáo Y học: Differential response of neuronal cells to a fusion protein of ciliary neurotrophic factor/soluble CNTF-receptor and leukemia inhibitory factor pot

... likely mediated by activation of the MAPK pathway and that this response is substantially independent of the JAK/STAT pathway DISCUSSION Fig STAT3 and MAPK activation by Hyper-CNTF in PC12 cells ... was obtained from Amersham International (Aylesbury, UK) X-ray ﬁlms (X-OMAT-AR) were from Eastman Kodak (Rochester, NJ) Cells, cytokines and antibodies PC12 and COS-7 cells (ATCC, Manassas, VA, ... out to be virtually as effective as NGF and Hyper-IL-6 to elicit neuronal differentiation STAT3 and MAPK activation by Hyper-CNTF in PC12 cells We then asked which signal transduction pathways are...

Báo cáo khoa học: A new clan of CBM families based on bioinformatics of starch-binding domains from families CBM20 and CBM21 potx

... 2.4.1.19 AAP31242 AAB65420 CAA55023 CAA48401 AAG31622 BAB91217 CAA33763 AAA22298 P31835 BAA14289 AAA22308 ALBSX1 AAA22310 AAA22309 CAA46901 BAA31539 AAA22239 CAA01436 Z34466 BAA02380 CAA41770 AAD00555 ... a- amylase amyCrysp a- amylase amyStrgr a- amylase amyStrlm a- amylase amyStrli1 a- amylase amyStrli2 a- amylase amyStrvi a- amylase amyThncu a- amylase amy_Aspaw a- amylase CBM2 0 (Purple of Fig.2) atrActsp ... nidulans Caenorhabditis elegans Caenorhabditis elegans Candida glabrata Candida glabrata Candida glabrata Candida glabrata Danio rerio Danio rerio Danio rerio Danio rerio wild-type Debaryomyces hansenii...

Report to the Congress on Practices of the Consumer Credit Industry in Soliciting and Extending Credit and their Effects on Consumer Debt and Insolvency pot

... BOARD OF GOVERNORS OF THE FEDERAL RESERVE SYSTEM Report to the Congress on Practices of the Consumer Credit Industry in Soliciting and Extending Credit and their Effects on Consumer Debt and Insolvency ... (FDIC), Report to the Congress on Practices of the Consumer Credit Industry 23 the National Credit Union Administration, the Office of the Comptroller of the Currency (OCC), and the Office of Thrift ... discussion of marketing and solicitation of credit cards is in Board of Governors, Further Restrictions on Unsolicited Offers Report to the Congress on Practices of the Consumer Credit Industry 21 The...

Báo cáo Y học:Association of the thyrotropin receptor with calnexin, calreticulin and BiP Effects on the maturation of the receptor docx

... enhances the degradation of TSHR (Fig 4) The association with CNX and/ or CRT therefore seems contribute to the stability and/ or the maturation of the TSHR To obtain further insights into the contribution ... amount of receptor was coimmunoprecipitated with CNX and CRT than with BiP Effects of interactions between TSHR and CNX and CRT We then attempted to determine the effects of these interactions on the ... chaperones and TSHR RESULTS Synthesis, maturation and degradation of TSHR at the endoplasmic reticulum level To study the maturation of TSHR after its synthesis, K562 cells stably transfected with...

Báo cáo sinh học: " Determination of suitable housekeeping genes for normalisation of quantitative real time PCR analysis of cells infected with human immunodeficiency virus and herpes viruses" pdf

... Reference gene selection for quantitative real- time PCR analysis in virus infected cells: SARS corona virus, Yellow fever virus, Human Herpesvirus-6, Camelpox virus and Cytomegalovirus infections Virol ... of cell types infected with a variety of strains of HIV-1, HSV-1, VZV and cytomegalovirus (CMV) compared to mock infected cells Results A range of cell lines and primary cells were infected with ... reliable housekeeping genes in cells infected with a variety of viruses [7,12], however to date no such study has been carried out in cells infected with human immunodeficiency virus (HIV) or the herpes...

báo cáo hóa học: " Effect of dexamethasone on quality of life in children with acute lymphoblastic leukaemia: a prospective observational study" pdf

... Health-related quality of life during post-induction chemotherapy in children with acute lymphoblastic leukemia in remission: An influence of corticosteroid therapy International journal of oncology ... dexamethasone on the pain subscale was 0.88 at T1 and 0.91 at T2, indicating more pain during the use of dexamethasone and repre- Table 1: Demographics Total N Males (%) Mean age (yrs) at diagnosis ... drafting of manuscript RL: coordination of the study, gathering and processing of data (questionnaires), performed the statistical analyses, drafting and revising of manuscript JH: participated...

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