Isolation and characterisation of suppressors of conditional histone mutants

... cerevisiae, each of the canonical core histones is encoded by two genes — histone H2A by HTA1 and HTA2; histone H2B by HTB1 and HTB2; histone H3 by HHT1 and HHT2; and histone H4 by HHF1 and HHF2 These ... complementation of histone H3 and H4 genomic deletion of cells expressing combinations of different histone H3 and histone H4 derivatives Observable AT phenotype of cells expressing combinations of different ... Some known sites of PTMs of histones 23 Some proposed functions of PTMs of core histones carried out by 24 different histone modifying enzymes PTMs of histone H4 N-terminal histone tail in different...

Isolation and characterization of an extracellular polymer closely related to flocculation of activated sludge

... natural activated sludge An extracellular polymer was extracted from an activated sludge suspension and was characterized because an extracellular polymer closely related to flocculation of activated ... drawn from the results of the study An extracellular polymer closely related to flocculation of activated sludge was obtained from the sludge suspension by sonication The polymer exhibited flocculating ... Blumenkrantz, N and Asboe-Hansen, G (1973) New method for quantitative determination of uronic acids Anal Biochem., Vol.54, 484-489 Dermlim, W., Prasertsan, P and Doelle, H (1999) Screening and characterization...

Tài liệu Báo cáo khoa học: Isolation and characterization of four type 2 ribosome inactivating pulchellin isoforms from Abrus pulchellus seeds docx

... Puriﬁcation of four pulchellin isoforms from A pulchellus seeds Using a combination of afﬁnity, ion exchange and chromatofocusing chromatography, four pulchellin isoforms were isolated from A pulchellus ... II, III and IV and refers to each pulchellin Characterization of four pulchellin isoforms isoform (P I, P II, P II and P IV) The A-chain of P II was formerly cloned and named recombinant pulchellin ... III and P IV isoforms from the eluate P III ⁄ P IV (Fig 1C) Isoelectric focusing gave pI of 5.8, 5.7, 5.5 and 5 .2 for the four isoforms respectively Secondary structure of the pulchellin isoforms...

Tài liệu Báo cáo khoa học: Phenol hydroxylase from Acinetobacter radioresistens S13 Isolation and characterization of the regulatory component docx

... aminoacids at the N-terminus of PHI (sequence: SKVYLALQDND) were compared with the sequences of the so called ‘intermediate components’ from two other PHs The N-terminal sequence of PHI from A radioresistens ... one PHR and one PHO (abc) protomer The hypothesis of a direct PHI phenol interaction is quite unlikely, because of the fact that the addition of phenol does not alter the emission spectrum of PHI ... oxygenase [28–30], and, in the case of Pseudomonas CF600 phenol hydroxylase, via a direct interaction with the substrate itself [9], in the case of A radioresistens S13 phenol hydroxylase, PHI...

Tài liệu Báo cáo khoa học: The isolation and characterization of cytochrome c nitrite reductase subunits (NrfA and NrfH) from Desulfovibrio desulfuricans ATCC 27774 Re-evaluation of the spectroscopic data and redox properties ppt

... describes the relationship between the heme core description and the spectroscopic and redox properties of each identiﬁed heme from the NrfHA complex Conclusions D desulfuricans ATCC 27774 ccNiR ... this communication, we report for the ﬁrst time the isolation and biochemical characterization of D desulfuricans ATCC 27774 ccNiR subunits The stoichiometry between NrfH and NrfA is discussed The ... (2003) Cytochrome c nitrite reductase ˜ from Desulfovibrio desulfuricans ATCC 27774 The relevance of the two calcium sites in the structure of the catalytic subunit (NrfA) J Biol Chem 278, 17455–17465...

Preparative isolation and purification of six volatile compounds from essential oil of Curcuma wenyujin using high-performance centrifugal partition chromatography doc

... (5) and b-elemene (6) (Fig 1) from the essential oil of C wenyujin GC-MS analysis showed that the six isolated compounds except curcumol (2) were the major components in the essential oil of C wenyujin ... total of mg of curdione (1), mg of curcumol (2), 10 mg of germacrone (3), 18 mg of curzerene (4), mg of 1,8-cineole (5) and 17 mg of b-elemene (6) from the essential oil (300 mg) in 2.4 mL of the ... 60–901C) of analytical grade was purchased from Uni-Chem (Guangzhou, China) Acetonitrile and acetone of chromatographic grade were purchased from Merck (Darmstadt, Germany) The essential oil of Curcuma...

Báo cáo Y học: Isolation and characterization of a thioredoxin-dependent peroxidase from Chlamydomonas reinhardtii doc

... highest identity with the BAS1 protein of Brassica, spinach, barley and A thaliana, PR1 of Phaseolus and MHF of A thaliana These proteins belong to the 2Cys-Prx subfamily All plant 2Cys-Prx proteins, ... very weak when Trx m from Chlamydomonas was used (data not shown), probably because of the weaker af®nity of Arabidopsis NTR for Trx m [21] Therefore, the ability of Trx m from Chlamydomonas ... protein and with BSA (400 lgámL)1) The reactions were stopped by adding 3.3 mM EDTA and analyzed on an agarose gel Thioredoxin-dependent peroxidase activity of Ch-Prx1 Peroxidase activity assays were...

Báo cáo khoa học: Isolation and characterization of a D-cysteine desulfhydrase protein from Arabidopsis thaliana pptx

... Nagasawa T, Ohkishi H, Kawakami B, Yamano H, Hosono H, Tani Y & Yamada H (1982) 3-chloro-d-alanine chloride-lyase (deaminating) of Pseudomonas putida CR 1–1 Puriﬁcation and characterization of ... molecular mass of 41.7 kDa and a pI of 6.34 The YedO protein from E coli and the d-CDes from Arabidopsis showed an overall identity of 36% and a similarity of 50% The blastp program in its default ... oxidases, transaminases, and racemases (epimerases) For example, in pea seedlings the occurrence of d-amino acid aminotransferase was demonstrated [36] For a number of other amino acids racemases...

Báo cáo khoa học: Isolation and characterization of an IgNAR variable domain speciﬁc for the human mitochondrial translocase receptor Tom70 potx

... degree of affinity and specificity for Tom70, and with a KD of nM compares very well with affinities reported for camelid VHH domains (that vary in the range 2–300 nM [36]) and for scFv and disulphide ... generate The human Tom70 receptor is an important example of such refractory targets, and the generation of a high-affinity reagent specific for the cytosolic domain is a potentially valuable tool in the ... one variant, designated 15Z-2, showed changes mapping to either the CDR or Ó FEBS 2003 An IgNAR variable domain specific for human Tom70 (Eur J Biochem 270) 3551 Fig Effect of removal of the 12F-11...

Báo cáo khoa học:The isolation and characterization of temperature-dependent ricin A chain molecules in Saccharomyces cerevisiae docx

... Temperature-dependent ricin A chain mutants Fig Stability of mutant ricin A chains The kinetics of protein degradation of (A) Kar2SP-RTAE177D and (B) Kar2SP-RTAF108L ⁄ L151P at all temperatures, or a cytosolic ... 5¢-ATATTCCCCAAACAATACCC-3¢ and the antisense primer CP133 5¢-TTAAAACTGTGACGATGGT GGA-3¢ with the TAA termination anticodon shown in bold Ampliﬁcation reactions were performed in a ﬁnal volume of ... 5589 Temperature-dependent ricin A chain mutants S C H Allen et al Fig Growth and viabilities of the conditional ricin A chain mutants (A) Transformed yeast cells were grown in liquid media at...

Báo cáo Y học: Isolation and characterization of MUC15, a novel cell membrane-associated mucin pot

... pair: 5¢-AATACCAAAGAAGCCTACAATG-3¢ and 5¢-GTACGAAGTGGAGGTATGTCATC-3¢ b Primer pair: 5¢-GCCATTT TAGGTGCTATTCTGG-3¢ and 5¢-TATTTTCTTTATCTGAGTTTA-3¢ c Primer pair: 5¢-CATCCATAGCAGATAACAGTC-3¢ and ... Ovarya Peripheral blood leukocytea Prostatea Small intestinea Spleena Testisa Thymusa Bone marrowa Fetal livera Lymph nodea Tonsila Breastb Lungb Milk cellsa Bovine Mammary glandc Mammary glandc ... degenerate primers Isolation of total RNA from the mammary gland of a lactating Danish Holstein cow was performed by means of an RNeasy kit (Qiagen, Hilden, Germany) Synthesis of cDNA was performed...

Báo cáo " Synthesis and characterisation of metallic nanoparticles " ppt

... Journal of Science, Mathematics - Physics 25 (2009) 221-230 In this paper, we report on synthesis and characterization of gold NPs as well as CoPt and FePt NPs Experimental Synthesis of gold ... under a mixture of % H2 and 95 % Ar atmosphere The increasing rate of temperature was 6°C/min and cooling with the furnace The sonoelectrodeposition method has been used for synthesis of FePt NPs ... Co and Pt atoms would continously deposited on the particles and at the end we would have a film of CoPt The presence of PVP around NPs created a steric forces that limit the growth of NPs and...

Báo cáo khóa học: Isolation and characterization of the Xenopus HIVEP gene family ppt

... combinations of degenerate and speciﬁc primers and by rescreening the cDNA libraries The partial clones of XHIVEP2 and -3 covered 5.9 and 3.2 kb of the respective 3¢ ends and included 3¢-UTRs and poly(A)-tails ... analyze the function of XHIVEP in the context of Xenopus embryogenesis (data not shown) However, the interpretation of the in vivo role of XHIVEP was not conclusive as the activator and repressor ... constructs by the generation of deletion mutants containing discrete functional domains of XHIVEP1 Thus, the cloning and characterization of the XHIVEP interacting factors would be of interest and should...

synthesis and characterisation of materials based on inorganic

... concentration of sodium to the surface, where it then carbonates A selection of typical powder X-ray diffraction patterns of the starting materials and polymers of different compositions is shown ... carbonate by atmospheric carbonation Polymer compositions which not cure satisfactorily show FTIR evidence of carbonation, by contrast with optimum curing compositions The water content of the ... carbonate and may disrupt the polymerisation process Reasons for the 316 V.F.F Barbosa et al / International Journal of Inorganic Materials (2000) 309 – 317 critical dependence of polymerisation...

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