Analysis of the multisubunit exocyst complex in schizosaccharomyces pombe

... Mechanisms of cytokinesis in S pombe 11 1.2.2.1 Positioning of the actomyosin ring in S pombe 11 1.2.2.2 Assembly of the actomyosin ring in S pombe 11 1.2.2.2.1 The role of myosin, light chains and ... assembly of the actomyosin ring The key components of the ring are actin and the motor protein myosin II (Schroeder, 1990) Type II myosins are important for the assembly of the actomyosin ring and ... of the actomyosin ring in the subsequent round of mitosis, indicating that the Myo2p spot is important for the assembly of the actomyosin ring 1.2.2.3 A SIN pathway regulating septation In S pombe...

Báo cáo khoa học: Analysis of the RNA degradosome complex in Vibrio angustum S14 potx

... degradation, the degradosome is also responsible for the processing of 5S ribosomal RNA and tRNAs, as well as the degradation of tmRNAs [4–8] The principal proteins comprising the degradosome include the ... degradosome complex in Vibrio angustum S14 have expanded on these results and shown that RhlB can interact with the CTH of V angustum S14 RNase E and the predicted RhlB-binding microdomain Interestingly, ... in V angustum S14 can self-associate Based on our observations, we present a model for the RNA degradosome complex in V angustum S14 which shows the positions of the microdomains in the CTH of...

URBAN AIR POLLUTION: AN ANALYSIS OF THE ENVIRONMENTAL KUZNETS CURVE IN ASIAN CITIES docx

... turning points to the use of aggregate air- quality data, which includes readings from both rural and urban areas, rather than the urban data used by Grossman and Krueger The turning-point for environmental ... graphs indicate the emergence of an “L-shape” EKC for air pollutants in Asian cities It is proposed that this is due to lowering of income turning points and the shortening of the stages of economic ... and consequential high levels of urban air pollution This could result in the majority of countries being near the EKC turning points or in the descending curve, creating the appearance of an...

Báo cáo khoa học: Biochemical analysis of the human EVL domains in homologous recombination doc

... contained the EVH2 domain (Fig 1A) EVL( 1–221) and EVL( 222– A B C We ﬁrst tested the ssDNA-binding and dsDNA-binding activities of EVL( 1–221) and EVL( 222–418), because the DNA-binding activity of EVL ... The EVH2 domain is responsible for the DNA-binding activity of EVL Preparation of EVL( 1–221) and EVL( 222–418) To identify the functional domain responsible for the recombination activity of human ... hand, the EVH1 domain binds to proteins containing the consensus sequence D ⁄ E FPPPPXD ⁄ E [21–23] The Pro-rich domain also binds to proﬁlin and proteins containing the SH3 and WW domains [17,24–26]...

Báo cáo khoa học: Experimental and steady-state analysis of the GAL regulatory system in Kluyveromyces lactis docx

... performance of the K lactis GAL switch when the autoregulation of KlGAL4 is eliminated, thereby mimicking the GAL system of S cerevisiae The broken line in Fig 5B shows the response of the KlGAL80 ... absence of autoregulation of Gal4 p in S cerevisiae reduces the burden of maintaining basal Gal4 p Thus, the gene duplication, shuttling of the repressor instead of the inducer and the absence of autoregulation ... of quantiﬁcation for the GAL system of K lactis is absent in the literature The GAL system in K lactis contains two regulatory genes (LAC9 or KlGAL4 and KlGAL80), a bifunctional gene KlGAL1 and...

Báo cáo hóa học: " A comprehensive analysis of the naturally occurring polymorphisms in HIV-1 Vpr: Potential impact on CTL epitopes" doc

... depending on their location, may impact on their binding inhibitors targeting these enzymes The viruses containing alterations may then be able to evade the inhibitory activities of the agents and are ... development of vaccines based on the auxiliary genes of HIV-1 Authors' contributions AS, VA, AK, AB, VS, RC and AC participated in the analysis of the predicted amino acid sequences of Vpr SM, DD and ... only one substitution This domain exhibits a total of 80 variant amino acids and 61 of them are of nonconservative in nature Several laboratories including ours have reported on the importance of...

báo cáo khoa học: " Expression profiling and integrative analysis of the CESA/CSL superfamily in rice" doc

... protein sequence similarity was determined by the MegAlign software 4.0 Genome-wide expression analysis of OsCESA/CSL and OsBC1L in rice and AtCESA/CSL and AtCOBL in Arabidopsis The expression profile ... Figure The overall expression of the family of CSLD genes is highest in the stamen and lowest in the shoot of seedlings with two tillers The total expression of the CSLA genes was highest in plumules ... among the members in a family although most of them exhibit different expression patterns Expression divergence of OsCESA/CSL genes in duplication We further observed the expression profiling of the...

báo cáo khoa học:" Quantitative analysis of the epithelial lining architecture in radicular cysts and odontogenic keratocysts" doc

... odontogenic keratocysts, R: radicular cysts Note the differences in the distribution of layer (the basal cell layer) across the keratocysts and radicular cysts and the tendency of radicular cysts ... differences in the epithelial architecture of OKs and radicular cysts and between the subtypes of OKs Radicular cysts have on average more layers and their number varies more than in OKs Furthermore, the ... discretisation of the cellular elements in the epithelial lining To this end, a method for theoretical cell segmentation in the epithelial compartment was applied, followed by an algorithmic grouping of the...

Biochemical characterization of the bi lobe complex in trypanosoma brucei

... Biochemical characterization of the bi- lobe complex in Trypanosoma brucei LADAN GHEIRATMAND A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY DEPARTMENT OF BIOLOGICAL SCIENCES ... Structural complexity of the bi- lobe 106 4.4 Characterization of bi- lobe associated proteins reveals extensive connection of bi- lobe to other cellular structures 108 4.5 TbSpef1 stabilizes the ... Biochemical characterization of the bi- lobe revealed a continuous structural network linking the bi- lobe to other singlecopied organelles in Trypanosoma brucei J Biol Chem 2013, Feb 1; 288 (5):3489-99...

A legal analysis of the cross border trade in telemedicine the singapore perspective

... trade The GATT was successful in eliminating tariffs because international agencies tracked and analysed trade flows The absence of service trade data creates a negotiating vacuum when the values ... participating nations on an approach to the international conflict of laws In sum, this paper looks at the main issues affecting the trade in telemedicine from both the international and the Singapore ... international trade, greater liberalisation of telemedicine services would allow the advanced health care economies to take advantage of their comparative advantage as well as move towards realising...

Báo cáo khoa học: Structural analysis of the jacalin-related lectin MornigaM from the black mulberry (Morus nigra) in complex with mannose ppt

... and that delineate the carbohydrate-binding cavity of the lectin protomer) A closer examination of the structure of 3728 the binding sites indicates that speciﬁcity of the Moraceae lectins is primarily ... β7 β1 N β10 N C D Fig The carbohydrate-binding site of MornigaM and other jacalin-related lectins (A) Ribbon diagram of the carbohydrate-binding site of MornigaM showing the three loops L1 (grey), ... Structure of a jacalin-related lectin complexed to mannose Fig Sequence and structure comparison of MornigaM to other jacalin-related lectins (A) Sequence alignment of MornigaM with jacalin as a...

Genetic analysis of the role of ARP2 3 complex in border cell migration in drosophila melanogaster

... polyproline sequences and interacts with profilin (Chang et al 1997) Since profilin binds to actin monomer, FH1 domains can bind the profilin-G-actin 13 Arp2/ 3 complex in border cell migration ... actin assembly In Drosophila, the Arp2/ 3 complex functions in actin dynamics together with SCAR/WAVE and WASP proteins The Drosophila Arp2/ 3 complex and SCAR/WAVE 22 Arp2/ 3 complex in border cell ... 1996) 36 Arp2/ 3 complex in border cell migration Lu Ruifeng 2010 The most obvious feature of the Mbm protein is a pair of C2HC zinc fingers, implicating a function of the protein in binding nucleic...

An analysis of the role of the schizosaccharomyces pombe homolog of survivin, bir1p, in mitosis

... bleaching of the GFP-Bir1p signal on the kinetochore results in the absence of fluorescence on the spindle mid-zone in anaphase B, indicating that the kinetochore-localized Bir1p translocates to the ... globular hinge domain in the middle In yeast, Smc1p and Smc3p are composed of an anti-parallel coiled-coil structure with the hinge domain at one end and the N- and C- termini together forming a ... arrangement is important for binding of various kinetochore components The flanking outer heterochromatin region is also important for protein binding In S pombe, elegant experiments depicted the...

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