Isolation and characterization of anticoagulant protein from the venom of hemachatus haemachatus (african ringhals cobra

Tài liệu Báo cáo khoa học: Phenol hydroxylase from Acinetobacter radioresistens S13 Isolation and characterization of the regulatory component docx

... aminoacids at the N-terminus of PHI (sequence: SKVYLALQDND) were compared with the sequences of the so called ‘intermediate components’ from two other PHs The N-terminal sequence of PHI from A radioresistens ... one PHR and one PHO (abc) protomer The hypothesis of a direct PHI phenol interaction is quite unlikely, because of the fact that the addition of phenol does not alter the emission spectrum of PHI ... oxygenase [28–30], and, in the case of Pseudomonas CF600 phenol hydroxylase, via a direct interaction with the substrate itself [9], in the case of A radioresistens S13 phenol hydroxylase, PHI...

Tài liệu Báo cáo khoa học: The isolation and characterization of cytochrome c nitrite reductase subunits (NrfA and NrfH) from Desulfovibrio desulfuricans ATCC 27774 Re-evaluation of the spectroscopic data and redox properties ppt

... describes the relationship between the heme core description and the spectroscopic and redox properties of each identiﬁed heme from the NrfHA complex Conclusions D desulfuricans ATCC 27774 ccNiR ... this communication, we report for the ﬁrst time the isolation and biochemical characterization of D desulfuricans ATCC 27774 ccNiR subunits The stoichiometry between NrfH and NrfA is discussed The ... (2003) Cytochrome c nitrite reductase ˜ from Desulfovibrio desulfuricans ATCC 27774 The relevance of the two calcium sites in the structure of the catalytic subunit (NrfA) J Biol Chem 278, 17455–17465...

Báo cáo khoa học: Isolation and characterization of a D-cysteine desulfhydrase protein from Arabidopsis thaliana pptx

... Nagasawa T, Ohkishi H, Kawakami B, Yamano H, Hosono H, Tani Y & Yamada H (1982) 3-chloro-d-alanine chloride-lyase (deaminating) of Pseudomonas putida CR 1–1 Puriﬁcation and characterization of ... molecular mass of 41.7 kDa and a pI of 6.34 The YedO protein from E coli and the d-CDes from Arabidopsis showed an overall identity of 36% and a similarity of 50% The blastp program in its default ... oxidases, transaminases, and racemases (epimerases) For example, in pea seedlings the occurrence of d-amino acid aminotransferase was demonstrated [36] For a number of other amino acids racemases...

Isolation and characterization of stem cell regulatory genes oct4 and stat3 from the model fish medaka

... Isolation and characterization of stem cell regulatory genes oct4 and stat3 from the model fish medaka LIU RONG (Master of Biological Sciences) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF ... Because of the essential roles of Oct4 and Stat3 in maintenance of stem cell pluripotency in mammals, and the unique features of the medaka, it seems equally important to understand the roles of Oct4 ... by Oct4 and Sox2 97 3.1.9.2.3 OSE alone is sufficient for regulated expression 3.2 Cloning and characterization of two isoforms of the medaka stat3 104 109 3.2.1 Isolation of stat3 cDNAs from the...

Tài liệu Báo cáo khoa học: Isolation and characterization of four type 2 ribosome inactivating pulchellin isoforms from Abrus pulchellus seeds docx

... Puriﬁcation of four pulchellin isoforms from A pulchellus seeds Using a combination of afﬁnity, ion exchange and chromatofocusing chromatography, four pulchellin isoforms were isolated from A pulchellus ... II, III and IV and refers to each pulchellin Characterization of four pulchellin isoforms isoform (P I, P II, P II and P IV) The A-chain of P II was formerly cloned and named recombinant pulchellin ... III and P IV isoforms from the eluate P III ⁄ P IV (Fig 1C) Isoelectric focusing gave pI of 5.8, 5.7, 5.5 and 5 .2 for the four isoforms respectively Secondary structure of the pulchellin isoforms...

Báo cáo Y học: Isolation and characterization of a thioredoxin-dependent peroxidase from Chlamydomonas reinhardtii doc

... highest identity with the BAS1 protein of Brassica, spinach, barley and A thaliana, PR1 of Phaseolus and MHF of A thaliana These proteins belong to the 2Cys-Prx subfamily All plant 2Cys-Prx proteins, ... very weak when Trx m from Chlamydomonas was used (data not shown), probably because of the weaker af®nity of Arabidopsis NTR for Trx m [21] Therefore, the ability of Trx m from Chlamydomonas ... protein and with BSA (400 lgámL)1) The reactions were stopped by adding 3.3 mM EDTA and analyzed on an agarose gel Thioredoxin-dependent peroxidase activity of Ch-Prx1 Peroxidase activity assays were...

Báo cáo khoa học: Isolation and characterization of an IgNAR variable domain speciﬁc for the human mitochondrial translocase receptor Tom70 potx

... degree of affinity and specificity for Tom70, and with a KD of nM compares very well with affinities reported for camelid VHH domains (that vary in the range 2–300 nM [36]) and for scFv and disulphide ... generate The human Tom70 receptor is an important example of such refractory targets, and the generation of a high-affinity reagent specific for the cytosolic domain is a potentially valuable tool in the ... one variant, designated 15Z-2, showed changes mapping to either the CDR or Ó FEBS 2003 An IgNAR variable domain specific for human Tom70 (Eur J Biochem 270) 3551 Fig Effect of removal of the 12F-11...

Báo cáo khoa học:The isolation and characterization of temperature-dependent ricin A chain molecules in Saccharomyces cerevisiae docx

... Temperature-dependent ricin A chain mutants Fig Stability of mutant ricin A chains The kinetics of protein degradation of (A) Kar2SP-RTAE177D and (B) Kar2SP-RTAF108L ⁄ L151P at all temperatures, or a cytosolic ... 5¢-ATATTCCCCAAACAATACCC-3¢ and the antisense primer CP133 5¢-TTAAAACTGTGACGATGGT GGA-3¢ with the TAA termination anticodon shown in bold Ampliﬁcation reactions were performed in a ﬁnal volume of ... 5589 Temperature-dependent ricin A chain mutants S C H Allen et al Fig Growth and viabilities of the conditional ricin A chain mutants (A) Transformed yeast cells were grown in liquid media at...

Báo cáo khóa học: Isolation and characterization of the Xenopus HIVEP gene family ppt

... combinations of degenerate and speciﬁc primers and by rescreening the cDNA libraries The partial clones of XHIVEP2 and -3 covered 5.9 and 3.2 kb of the respective 3¢ ends and included 3¢-UTRs and poly(A)-tails ... analyze the function of XHIVEP in the context of Xenopus embryogenesis (data not shown) However, the interpretation of the in vivo role of XHIVEP was not conclusive as the activator and repressor ... constructs by the generation of deletion mutants containing discrete functional domains of XHIVEP1 Thus, the cloning and characterization of the XHIVEP interacting factors would be of interest and should...

Báo cáo khoa học: " Isolation and characterization of Streptococcus sp.from diseased flounder (Paralichthys olivaceus )in Jeju Island" ppt

... eht fo esahp suoeuqa reppu eht no detcelloc saw AND lairetcaB nim rof 000,6 ta degufirtnec dna nim rof sllec lairetcab gniliob yb detcartxe neht saw AND lairetcab ;retaw dellitsid elbuod dezilirets ... 817( rof evitisop ,9~4 senal ;)pb 001,1( rof evitisop ,3 dna senal ;noitcefni laccocotperts rof evitagen ,1 enal ;lortnoc evitagen ,N enal ;)pb 003 ,M4040 ( lortnoc evitisop ,P senal ;reddal AND ... enilav ,esapil rof snoitcaer evitagen dah setalosi llA nispyrtomyhc -α dna esatahpsohp enilakla rof snoitcaer evitisop dah fo setalosi 81 dna esadinimasoculg-β-lyteca -N dna esaretse rof snoitcaer...

Isolation and characterization of highly pathogenic avian influenza virus subtype H5N1 from donkeys docx

... Cite this article as: Abdel-Moneim et al., Isolation and characterization of highly pathogenic avian influenza virus subtype H5N1 from donkeys Journal of Biomedical Science 2010, 17:25 ... Fatal avian influenza A H5N1 in a dog Emerg Infect Dis 2006, 12:1744-1747 11 Li HY, Yu K, Yang H, Xin X, Chen J, Zhao P, Bi Y: Isolation and characterization of H5N1 and H9N2 influenza viruses from ... Experimental infection of cattle with highly pathogenic avian influenza virus (H5N1) Emerg Infect Dis 2008, 14:1132-1134 17 Cardona CJ, Xing Z, Sandrock CE, Davis CE: Avian influenza in birds and mammals...

báo cáo khoa học: " Involvement of S-adenosylmethionine-dependent halide/thiol methyltransferase (HTMT) in methyl halide emissions from agricultural plants: isolation and characterization of an HTMT-coding gene from Raphanus sativus (daikon radish)" ppt

... Figure analysis of methyl halides, methanethiol, and DMS from R sativus GC-MS GC-MS analysis of methyl halides, methanethiol, and DMS from R sativus (a) GC-MS spectrum of methyl halide standards ... the emission profiles of methyl halides from some plants and the characterization of the enzymatic properties of recombinant R sativus HTMT (RsHTMT) Results and discussion Wuosmaa and Hager [14] ... substrate for HTMT in A thaliana Cloning and sequence analysis of an HTMT coding gene from R sativus To investigate the properties of HTMT in R sativus, a full length HTMT-encoding gene (Rshtmt) was...

Báo cáo khoa học: "Isolation and characterization of a genotype 4 Hepatitis E virus strain from an infant in China" ppt

... Bezanilla JC, Toth TE, Meng XJ: Identification of genotype hepatitis E virus (HEV) in serum and faecal samples from pigs in Thailand and Mexico, where genotype and HEV strains are prevalent in ... genotype HEV strains with complete genome available in GenBank (including both swine and human original isolates) were used as references in the analysis; their GenBank accession numbers and source ... aimed to determined the whether this genotype HEV strain is prevalent in Shanghai swine or not HEV paly an important role in acute sporadic and epidemic viral hepatitis in Asia and Africa Although...

Báo cáo y học: "Isolation and characterization of microparticles in sputum from cystic fibrosis patients" pot

... analysis of inflammatory cells infiltrating the cystic fibrosis airway mucosa Clin Exp Immunol 2001, 124(1):69-76 35 Livraghi A, Randell SH: Cystic fibrosis and other respiratory diseases of impaired ... largely sophisticated and quantifiable, and the search for novel biomarkers of CF airways disease in this biofluid is under way [16,47] The finding of MPs in sputum raises some intriguing questions ... endothelial cells, granulocytes, monocytes, lymphocytes) following chemical (cytokines, thrombin and endotoxin), physical (shear stress and hypoxia) [17] and apoptotic [18] stimuli One of the first described...

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